Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,714	C→T	G184G (GGC→GGT)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,714	0	C	T	95.6%	192.3 / NA	70	G184G (GGC→GGT)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (34/31);  minor base A (3/0);  total (38/32)
Fisher's exact test for biased strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCA  >  NC_000913/12472‑12960                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       gAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCa                                                                                                                                                                                                                                                 >  1:102943/1‑250 (MQ=255)  aagtaaaagcgaaaaaaaGGGCCCCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAATCCGCTGAAGATTACCTGGGGGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGGCAGGCCACCAAAGACGCAGGCCGTATCTCTGGTCTGGAAGTAAAACGTATCATCAACGATCCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGTCAAc                                                                                                                                                                                                                                               <  2:445829/237‑1 (MQ=255)  aaGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAAc                                                                                                                                                                                                                                               >  1:557460/1‑251 (MQ=255)     ttAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGt                                                                                                                                                                                                                                            >  1:55209/1‑251 (MQ=255)      aaaaGGCAAAAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTa                                                                                                                                                                                                                                           <  2:263312/250‑1 (MQ=255)         aGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACt                                                                                                                                                                                                                                         >  1:5110/1‑250 (MQ=255)            ccAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATcg                                                                                                                                                                                                                                     <  1:204849/251‑1 (MQ=255)                    aggCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGAACTGGCAAACGTACTATCGCGGtttct                                                                                                                                                                                                                             >  2:166672/2‑249 (MQ=255)                     ggCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATg                                                                                                                                                                                                                            >  1:349468/1‑251 (MQ=255)                        aCCGCCGCAGATCTCTGCTGAAGTGCTGCAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAATCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGACTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACCAAGGTACTGGCAACCGTACTATCGCGGTTTATGAcc                                                                                                                                                                                                                         >  2:482719/1‑251 (MQ=255)                                      atgctgAAGTGCTGAAAAAAATGAAGAAAACCGCGGAAGATTACCTGGGTGAACCGGTAACTGAAGTTGTTATCACCGTACCGGCATACTTTAACGATGCTCATCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTAc                                                                                                                                                                                                            <  1:363655/249‑1 (MQ=255)                                       tgctgAAGGGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACtt                                                                                                                                                                                                          >  1:249344/1‑251 (MQ=255)                                          tgAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGCACCGGTAACTGAAGCTGTTATCACCGTACCGGAATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCg                                                                                                                                                                                                       >  1:62050/1‑251 (MQ=255)                                           gAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCg                                                                                                                                                                                                       >  1:459329/1‑250 (MQ=255)                                           gAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTAACTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTCTATGACCTGGGTGGTGGTACTTTCg                                                                                                                                                                                                       <  2:459329/250‑1 (MQ=255)                                            aaGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGat                                                                                                                                                                                                     >  2:198382/1‑251 (MQ=255)                                            aaGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGat                                                                                                                                                                                                     <  1:572300/251‑1 (MQ=255)                                              gTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCTACCGCAGCTGCGCTGGCTTACGGGCTGGACAAAGGGACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGatat                                                                                                                                                                                                   <  1:36059/251‑1 (MQ=255)                                                gCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTTCCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCTCAGCTGCTCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGTGTGGTGGGACTTTCTATAtt                                                                                                                                                                                                  <  2:438960/250‑1 (MQ=255)                                                   gAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTGAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCta                                                                                                                                                                                              <  2:5110/251‑1 (MQ=255)                                                       aaaaTGAAGAAAACAGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGCTATTTCtattat                                                                                                                                                                                          >  2:116228/1‑251 (MQ=255)                                                          aTGAAGAAAACCGCTCAAGATTACCGGGGTGAACCGGTAGCTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAATAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACTGTCTGGACAAAGGTTCTGGCAACCGTACTTGCGCGGTTTATGACCTGGGTGGTTTTACTTTCGATATCTCTATTATCGa                                                                                                                                                                                       <  2:312717/251‑1 (MQ=255)                                                                  aaCCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGTTCTTTCTATTATCGAAATcga                                                                                                                                                                                 >  2:352166/1‑249 (MQ=255)                                                                        tGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTg                                                                                                                                                                         >  1:53247/1‑251 (MQ=255)                                                                        tGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTg                                                                                                                                                                         >  2:170743/1‑251 (MQ=255)                                                                                     ggTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAAc                                                                                                                                                            <  1:548879/251‑1 (MQ=255)                                                                                      gTGAACCGGTAACTGAAGCTGTTAGCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTAGCGAAATCGACGAAGTTGACGGCGAAAAAAcc                                                                                                                                                           <  2:417011/251‑1 (MQ=255)                                                                                       tGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAACGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGACAAAACCt                                                                                                                                                          >  1:563709/1‑251 (MQ=255)                                                                                           ccGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCg                                                                                                                                                       >  1:300814/1‑250 (MQ=255)                                                                                              gTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGt                                                                                                                                                   <  1:331672/251‑1 (MQ=255)                                                                                                      aGCTGTTATAACCGAACCGGCATACTTTAACGATAAGCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGACTGGAAGAAAAACGTATCATCAACGTACAGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACAGTAATATCGCGGTTTATGACCTGGGGGGTGGTACTTTAGATATTTATATTATCGAAATCGACGAAGTTGGCGGCAAACAAACATTCGAAGTTCTGGcac                                                                                                                                           >  2:412604/1‑250 (MQ=255)                                                                                                          gTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTACCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGTACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATAGTTCTATTATCGAAATCGACGAAGTTGACGGCTAATAAGCCTTCGAAGGTCTTGcaacc                                                                                                                                         <  2:243176/249‑1 (MQ=255)                                                                                                                 ccGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGAt                                                                                                                                >  1:80329/1‑251 (MQ=255)                                                                                                                     aCCGGCATACTTTACCGATGCTCAGCGTCCGGCGACCACCGACGCCGGCCGTATCGCTGGTCTGGAAGTAAAACGAATCATCAACGAACTGACCGCGGCTGGGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACATGGGTCGGGGAACTTTCGATATTTCTATTATCGCAATCGCCGAAGTGGACGGCGTAAAATAATTCGAAGGTCTGGaaacgagaggtgttaccc                                                                                                                            >  2:86622/1‑237 (MQ=255)                                                                                                                     aCCGGCATACTTTAACGAGGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATAccc                                                                                                                            <  2:487127/251‑1 (MQ=255)                                                                                                                          cATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTg                                                                                                                       >  1:57527/1‑251 (MQ=255)                                                                                                                          cATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTg                                                                                                                       <  1:231090/251‑1 (MQ=255)                                                                                                                             aCTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTGGACGGCGAACACACCTTCGAAGTTCTGGCAACCAACGGTGCTACCCACCTgggt                                                                                                                    >  2:535891/1‑250 (MQ=255)                                                                                                                                  aaCGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGgtggtg                                                                                                                >  2:482590/1‑250 (MQ=255)                                                                                                                                      gtgcacAGCGTCAGGCCACCAAAGATGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACTGTTTGGACAAAGGTACTGTCAACCGTACTAGCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGAc                                                                                                           <  2:23396/246‑1 (MQ=255)                                                                                                                                        gCTCAGAGTCAGGCAACCAAAGACGCAGACCGTATCGCTGGTCTGGAAGTAAAACGTATCATAAACGAACCGACCGCAGATGCGCTGGCTTACGGTCTGGACAAAGGAACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGGACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATCCCCACCTGGGTGGGGAAGACtt                                                                                                         >  2:550981/1‑251 (MQ=255)                                                                                                                                           cAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCg                                                                                                       >  2:543804/1‑250 (MQ=255)                                                                                                                                               gTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGc                                                                                                  >  2:41494/1‑251 (MQ=255)                                                                                                                                                      aaCCAAAGACGCAGGCCGTATCGCTGGTCTTGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACATAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATCAGCGAAATCGACGAAGTTGACGGCGAAAAAACCTTc                                                                                                                                                        <  2:445118/190‑1 (MQ=255)                                                                                                                                                      aaCCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGGACTGGCACCCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGAGGGCGAAAAAACCTTc                                                                                                                                                        >  1:445118/1‑190 (MQ=255)                                                                                                                                                       aCCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGAt                                                                                          >  1:390946/1‑251 (MQ=255)                                                                                                                                                       aCCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGAAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGAACGCCGTCTGAt                                                                                          >  1:250862/1‑251 (MQ=255)                                                                                                                                                          aaaGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGggt                                                                                                                                                                                                                    >  2:74263/1‑126 (MQ=255)                                                                                                                                                          aaaGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGggt                                                                                                                                                                                                                    <  1:74263/126‑1 (MQ=255)                                                                                                                                                            aGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACt                                                                                     >  1:265496/1‑251 (MQ=255)                                                                                                                                                                   ggCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGt                                                                              <  1:543804/251‑1 (MQ=255)                                                                                                                                                                       gTATCGCTGGTCTGGAAGTTAAACGTATCATCAACGAACCGACAGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTga                                                                           >  1:485689/1‑250 (MQ=255)                                                                                                                                                                                gTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCTCCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTCCCTGGTTGAAGAATTCaag                                                                 <  2:18440/251‑1 (MQ=255)                                                                                                                                                                                    ggAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTTCTGTCTGGACAAAGGTTCTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGACATCGACGAAGTTGACGGCGAAAAAACCGTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGTCTTCGACAGCCCTCTTATCAACTACCTGGTTGAACAATTCaagaa                                                               <  1:378525/249‑1 (MQ=255)                                                                                                                                                                                     gAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTTAAGACTGCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCaagaaaga                                                            <  1:47119/251‑1 (MQ=255)                                                                                                                                                                                       aGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATc                                                          <  1:198382/251‑1 (MQ=255)                                                                                                                                                                                        gTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATc                                                          >  1:625801/1‑250 (MQ=255)                                                                                                                                                                                          aaaaCGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTGCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAgg                                                       <  2:55209/251‑1 (MQ=255)                                                                                                                                                                                                   catcaACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGAc                                               <  2:390946/250‑1 (MQ=255)                                                                                                                                                                                                      caACGAACCGACCGCAGCTGCGCTGGGTTACGGTCTTGACAAAGGTACTGGCAACCGTACTATCGCGGTTGATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTGCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTg                                            <  1:84621/250‑1 (MQ=255)                                                                                                                                                                                                       aaCGACCCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCCACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATAGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATAACCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTgcg                                          >  2:206573/1‑251 (MQ=255)                                                                                                                                                                                                          gAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGAACTGGCACCCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGCGAAGACTTCGACAGCCGTCCGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCa                                        >  2:443930/1‑250 (MQ=255)                                                                                                                                                                                                               gaccgCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCCACGGTGATa                                                                                                                               >  1:4162/1‑158 (MQ=255)                                                                                                                                                                                                               gaccgCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACGATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGAGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATa                                                                                                                               <  2:4162/158‑1 (MQ=255)                                                                                                                                                                                                                   gCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCt                              <  1:612287/251‑1 (MQ=255)                                                                                                                                                                                                                   gCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGGATTGACCTGCGCAACGATCCGCt                              <  2:102943/251‑1 (MQ=255)                                                                                                                                                                                                                                cTTACGGTCTCGACAAAGGTACTGGCAACCGTACTATCGCGGTTTGTGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGCCGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGGTACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGCTGAAGAATTCAAGAAAGCTCAGGGGATTGACCTGCGCAACGCTCCGCTGGCAATGCAgcgc                 >  2:274912/1‑251 (MQ=255)                                                                                                                                                                                                                                       tCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGCCGAAGTTGACGGCGAACAAACCTTCGAAGTTCTGGCCACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGCATTCAAGAAAGATCAGGGTATTGACCTGAGCAACGATCCGCTGGCAATGCAGCGCCTgca            >  2:543454/1‑247 (MQ=255)                                                                                                                                                                                                                                           gACAAAGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGc      <  2:567615/251‑1 (MQ=255)                                                                                                                                                                                                                                               aaGGTACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGGTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAGACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCa  <  2:557460/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACCTGGGTGGTGGTACTTTCGATATTTCTATTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAGACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCA  >  NC_000913/12472‑12960

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑