Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	12,507	T→C	I115I (ATT→ATC)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	12,507	0	T	C	100.0%	148.4 / NA	47	I115I (ATT→ATC)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/19);  total (28/19)

ATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACC  >  NC_000913/12260‑12745                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               aTCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATctct                                                                                                                                                                                                                                             >  1:336696/1‑251 (MQ=255)  tCGCACCACGCCTACTATCATTGCCTATACCCCGGCTGGTGAAACTCTGGTTGGTCAGCCGGCTAAAAGTCAGGCAGTGAAGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGCCGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCCAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCAAGAAAATGGCACCGCCGCAGATCTctg                                                                                                                                                                                                                                            >  2:36059/1‑251 (MQ=255)   cgcccaacGCCTTCTACTATTGCCTATACCCAGGATGGTGAAACTCTGGTTGGGCAGCCGGCTAAACGTCAGGCAGTGACGACCCCGCAAACCACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGGGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATACCGGCGCCGCAGGGTTCGAAGTAAAAGGCCAGAAAAGGTCTCCGCCGCAGATCTctgc                                                                                                                                                                                                                                           <  2:402940/245‑1 (MQ=255)                        gCCTATACCCCGGGTGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCGGGACGACGAAGTACAGCGTGATGTTTCCATCATGCCGTTCACAATTATTGCTGCTGATAACGGCGACGCATGGGTCGGAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATga                                                                                                                                                                                                                       >  2:84621/1‑250 (MQ=255)                                ccAGGATGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGAGACGCACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAACTTATTGCTGCTGATACCGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCg                                                                                                                                                                                                              >  1:312717/1‑251 (MQ=255)                                   ggATGGTGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGCAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGACGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTg                                                                                                                                                                                                           >  2:231090/1‑251 (MQ=255)                                         tGAAACTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGAtt                                                                                                                                                                                                     >  1:501997/1‑251 (MQ=255)                                           aaaCTCTGGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACCCCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTAc                                                                                                                                                                                                   <  1:525592/251‑1 (MQ=255)                                                    ttGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTACTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGa                                                                                                                                                                                           >  1:417011/1‑250 (MQ=255)                                                     tGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAAc                                                                                                                                                                                         >  1:263312/1‑251 (MQ=255)                                                      ggccAGCCGGCTAAACGTCAGGATGTGACGAACCCCAAAAACCCCCTGTTTGCGATAAAACTCCTGATGGGCCGCCGCTTCCAGGACAAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGCCGCATGGGTCGAAGTTAAAGGCCAAAAAATGGCACCGCCGCAGAGCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAAc                                                                                                                                                                                         <  2:395852/247‑1 (MQ=255)                                                      ggTCATCCGGCTAACCGTAAGGCAGTGACGACCCCGCAAAACACCCGGTTGGCGATTAACCGCCTGATTGTCCGCCGCTCCCAGGACGAAAAAGTACAGCGTGATGTTTCAATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAAcc                                                                                                                                                                                        <  1:616001/251‑1 (MQ=255)                                                      ggTCAGCCGGCTAAACGTCAGACAGTGACGGAACCGCAAAACCCCATGTTTGCGCTTAAACGACTGATTGGCCGCCGCTTCCAGGACGAAGAAGAACCGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGACAAAAATGAAGAAAAACGCTGAAGATTACATGGGTGAAcc                                                                                                                                                                                        >  2:591604/1‑251 (MQ=255)                                                         cAGCCGGCTAAACGCCAGGCAGTGCCGAACCCGCAAAACCCCCTGTTTGCGATTAAACGCCGGATTGGCCGCCGTTTCCAGGACGAAGAAGTACAGCGGGATGTTTCCATCATGCCGTTCAAAATTATTGCGGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCAAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGt                                                                                                                                                                                     <  2:501997/251‑1 (MQ=255)                                                                     cGTCAGGAAGTGACGAACCCGCAAAACACCCTGTTTGCGATTAAACGACTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGAACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGt                                                                                                                                                                         >  2:393122/1‑251 (MQ=255)                                                                                   gAACCCGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAACATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCgg                                                                                                                                                           >  1:281324/1‑251 (MQ=255)                                                                                     ccccGCAAACCCCCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTGCCAGGACAAAGAAGTACAGCGTGATGTTTCCATCATGCCTTTCAAAATTATTGCGGCTGATACCGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCa                                                                                                                                                         <  1:581148/250‑1 (MQ=255)                                                                                       ccGCAAAACACCCTGTTTGCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCGAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCAt                                                                                                                                                        >  1:487127/1‑250 (MQ=255)                                                                                                       tggCGATTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGTTGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCa                                                                                                                                        <  1:470419/248‑1 (MQ=255)                                                                                                            aTTAAACGCCTGATTGGCCGCCGCTTCCAGGACGAAGAAGTACAGGGTGATGTTCCCACCAGGCCTTTCAAAATTATTGCGGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCa                                                                                                                                  <  1:266906/251‑1 (MQ=255)                                                                                                                         ttGGCCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCTTGCCGTTCCAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGCAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGAc                                                                                                                     >  2:47119/1‑251 (MQ=255)                                                                                                                           ggccgccgcTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGc                                                                                                                   >  2:204849/4‑251 (MQ=255)                                                                                                                                ccgcTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGAAAACGGCGACGCATGGGTCGAAGTTAAAGGCAAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGcc                                                                                                              <  2:146957/251‑1 (MQ=255)                                                                                                                                      ccAGGAGGAAGAAGAACAGGTTAATGTTTCAATCATGCCGTGCAAAATTTGTGCGGCTAATAACGTCGCCGAAGGGGTGAAAGTTAAAGGCCAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCTCCGTACCGGCATACTTTAACGATGCGCAGCGTCAGGCAACCAAAGACGCAGGCCTTATCg                                                                                                        <  2:585014/251‑1 (MQ=255)                                                                                                                                      ccAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCCTGGGTCGAAGTTAAAGGCCCGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAAATGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACACAGGCCGTATCg                                                                                                        >  1:536695/1‑251 (MQ=255)                                                                                                                                      ccAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCg                                                                                                        >  1:490508/1‑251 (MQ=255)                                                                                                                                                  agTACAGCGTGATTTTCCCATCATGCCTTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAATTTAAAGCCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAg                                                                                            <  1:260070/251‑1 (MQ=255)                                                                                                                                                       aGCGTGATGTTTACATCATGCCGTTCAAAATTATTGCTGCAGATAACGACGACCCATGGGTCGACGTTAAAGACCAGACAATGGCACCGCCGCAGGTCTCTGCTGAAGAGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGAACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGAAGGCCGGATCGCTGGTCTGGAAGTaaa                                                                                        >  2:294944/1‑250 (MQ=255)                                                                                                                                                                  tCCATCATGCTGTCAAAAATGAATGCTGTGGATAACGGCGACGCGTGGGTCGAAGTGAAAGACCAAAAAAGGGCACCTCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGTAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTAGCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTAtcatca                                                                             <  1:618043/250‑1 (MQ=255)                                                                                                                                                                      gcatGCCTTTAAAAATTGTGGCTGCTGATACCGGCGACCAATGGGTGAAATTTAAGGGCAAAAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTTCCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCGTACTTTAACGATGCTCAGGTTCATGCATCCAAAGACGCGGGCCGTATCGCTGGTCTGGTAGTAAAACGTAGCATCAACg                                                                          <  2:242924/248‑1 (MQ=255)                                                                                                                                                                       catGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAac                                                                       >  2:456812/1‑251 (MQ=255)                                                                                                                                                                         ggCCGTTAAAAATTATGGCTGCTGATAAGGGGGACGCATGGGTCGAAGTTAAAGGCAAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAaccg                                                                     <  2:627541/250‑1 (MQ=255)                                                                                                                                                                                 aaaaTTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGATCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGAACGCAGc                                                             >  1:222136/1‑251 (MQ=255)                                                                                                                                                                                             gctgATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTa                                                 <  1:73563/251‑1 (MQ=255)                                                                                                                                                                                                         gACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACa                                      >  1:567615/1‑250 (MQ=255)                                                                                                                                                                                                            gCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGAACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAgg                                  >  1:299716/1‑251 (MQ=255)                                                                                                                                                                                                                     gAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCa                          >  1:102943/1‑250 (MQ=255)                                                                                                                                                                                                                      aagtaaaagcgaaaaaaaGGGCCCCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAATCCGCTGAAGATTACCTGGGGGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGGCAGGCCACCAAAGACGCAGGCCGTATCTCTGGTCTGGAAGTAAAACGTATCATCAACGATCCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGTCAAc                        <  2:445829/237‑1 (MQ=255)                                                                                                                                                                                                                      aaGTTAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAAc                        >  1:557460/1‑251 (MQ=255)                                                                                                                                                                                                                         ttAAAGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGt                     >  1:55209/1‑251 (MQ=255)                                                                                                                                                                                                                          aaaaGGCAAAAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTa                    <  2:263312/250‑1 (MQ=255)                                                                                                                                                                                                                             aGGCCAGAAAATGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACt                  >  1:5110/1‑250 (MQ=255)                                                                                                                                                                                                                                ccAGAAAAGGGCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATcg              <  1:204849/251‑1 (MQ=255)                                                                                                                                                                                                                                        aggCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGAACTGGCAAACGTACTATCGCGGtttct      >  2:166672/2‑249 (MQ=255)                                                                                                                                                                                                                                         ggCACCGCCGCAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGTACTGGCAACCGTACTATCGCGGTTTATg     >  1:349468/1‑251 (MQ=255)                                                                                                                                                                                                                                            aCCGCCGCAGATCTCTGCTGAAGTGCTGCAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAATCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGACTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACCAAGGTACTGGCAACCGTACTATCGCGGTTTATGAcc  >  2:482719/1‑251 (MQ=255)                                                                                                                                                                                                                                              cgccgcAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAg                                                                                                                 <  1:208524/138‑1 (MQ=255)                                                                                                                                                                                                                                              cgccgcAGATCTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAg                                                                                                                 >  2:208524/1‑138 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               ATCGCACCACGCCTTCTATCATTGCCTATACCCAGGATGGTGAAACTCTAGTTGGTCAGCCGGCTAAACGTCAGGCAGTGACGAACCCGCAAAACACTCTGTTTGCGATTAAACGCCTGATTGGTCGCCGCTTCCAGGACGAAGAAGTACAGCGTGATGTTTCCATCATGCCGTTCAAAATTATTGCTGCTGATAACGGCGACGCATGGGTCGAAGTTAAAGGCCAGAAAATGGCACCGCCGCAGATTTCTGCTGAAGTGCTGAAAAAAATGAAGAAAACCGCTGAAGATTACCTGGGTGAACCGGTAACTGAAGCTGTTATCACCGTACCGGCATACTTTAACGATGCTCAGCGTCAGGCAACCAAAGACGCAGGCCGTATCGCTGGTCTGGAAGTAAAACGTATCATCAACGAACCGACCGCAGCTGCGCTGGCTTACGGTCTGGACAAAGGCACTGGCAACCGTACTATCGCGGTTTATGACC  >  NC_000913/12260‑12745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑