Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	11,695	G→A	A31V (GCC→GTC)	yaaI ←	DUF2541 domain‑containing protein YaaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	11,695	0	G	A	100.0%	197.5 / NA	64	A31V (GCC→GTC)	yaaI	DUF2541 domain‑containing protein YaaI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/38);  total (26/38)

GAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGA‑TTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTG  >  NC_000913/11455‑11940                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       gagaCGCAGCGTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGCAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCgagg                                                                                                                                                                                                                                                >  1:250128/1‑249 (MQ=255)  agaCGCAGCGTTTATTGTCGTTAACACGGTTAATGATGATCCAGTCAGTGGTTTGCACTTCTTTTATTTCTGAAGGAATATTCAGACGATGACTGGCGATACGGGCGGAATTGAAATAAACCGATGCACAGCTTAACTGAAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTGTCAGCGCCAGAGCGTTGGTTTCGTAACGCGGCATTGTAATGACGCCGagtagt                                                                                                                                                                                                                                             <  2:157769/251‑1 (MQ=255)        atcGTTTATTGTCGTTATCACTGTTAATGTTGATCCATGCAGTTGTTTGGTCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGTGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAAGCGCCATGTTCGTCAGTTAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCTAGATCGTTGTTATCGGTACGCGGCATTGCAATGACGCCGAGGATTATATgg                                                                                                                                                                                                                                       <  2:628826/249‑1 (MQ=255)           gTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCg                                                                                                                                                                                                                                    >  1:431747/1‑251 (MQ=255)           gTTTATTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCg                                                                                                                                                                                                                                    >  1:418975/1‑251 (MQ=255)               aTTGTCGTTATCACTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTg                                                                                                                                                                                                                                >  1:110387/1‑251 (MQ=255)                 ggTCGTTATCCCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGcc                                                                                                                                                                                                                              <  2:340306/250‑1 (MQ=255)                          cacTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTg                                                                                                                                                                                                                     <  1:29547/249‑1 (MQ=255)                             tGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTg                                                                                                                                                                                                                            >  1:502534/1‑241 (MQ=255)                             tGTTAATGGTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTg                                                                                                                                                                                                                            <  2:502534/241‑1 (MQ=255)                                                   ggTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCa                                                                                                                                                                                            <  2:130614/251‑1 (MQ=255)                                                                 ttATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATTACGCCGAGGAGTTTATGGTCGTTTTCCTGCGCCGGGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAAt                                                                                                                                                                               <  2:250912/250‑1 (MQ=255)                                                                  tATTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATc                                                                                                                                                                              <  1:478068/250‑1 (MQ=255)                                                                   aTTTCTGAAGGAATATTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGTAAATCg                                                                                                                                                                             <  2:418975/250‑1 (MQ=255)                                                                         gAAGGAATATTCAGACGCTGACTGGCGCTACGGGCGGCTTGGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGTTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGGTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTaaaa                                                                                                                                                                        <  1:345837/249‑1 (MQ=255)                                                                            ggAATGTTCAGACTCTGACTTGCGCGACTGGCGGCTTTGAAATAAACCGATGTACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGGTTCACAATGCGACAAACAGGAAGTTTCAGCGCCATATCGTTGGTTTCGTTACGCCGCATTGCAATGACGCCGAGTAGTGTATGGTCGTTTGCCTGCGCCGTGCATCACAGCATCATGCTAATCGCCAGGCTGGCGGAAATCGTAACAACGGa                                                                                                                                                                   <  2:525166/251‑1 (MQ=255)                                                                               atatTCAGACTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGAttt                                                                                                                                                                >  2:160355/1‑251 (MQ=255)                                                                                     agactctGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATa                                                                                                                                                            >  1:20580/4‑249 (MQ=255)                                                                                      gactctGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTACAAACGGCTTTCCTAAgg                                                                                                                                                         >  2:56414/3‑251 (MQ=255)                                                                                               tGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGTCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCtt                                                                                                                                                 >  1:133714/1‑250 (MQ=255)                                                                                                    cTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGGAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTggg                                                                                                                                           >  2:83257/1‑251 (MQ=255)                                                                                                      aCGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACCAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGAATCTCTTAGTGGGa                                                                                                                                          >  2:443082/1‑250 (MQ=255)                                                                                                                 tGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGGATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAgagg                                                                                                                               <  1:22100/250‑4 (MQ=255)                                                                                                                     aTAAACCGATGCACCGCTTAACTGTAAGTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATg                                                                                                                           >  1:160527/1‑250 (MQ=255)                                                                                                                        aaCCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATa                                                                                                                       <  2:224829/251‑1 (MQ=255)                                                                                                                             aTGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAACTCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAagcgg                                                                                                                                     >  2:336749/1‑229 (MQ=255)                                                                                                                             aTGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAgg                                                                                                                                     <  1:336749/232‑1 (MQ=255)                                                                                                                              tGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGCGTTGTATGCGTTTCACAATGCGACAAACAGGCAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCa                                                                                                                                                              >  2:610147/1‑206 (MQ=255)                                                                                                                              tGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAAGGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCa                                                                                                                                                              <  1:610147/206‑1 (MQ=255)                                                                                                                                cACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACt                                                                                                                 >  1:121689/1‑249 (MQ=255)                                                                                                                                   cGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTATTGGGAAGAGGTAGAGGGATGCATACCCAcacgttt                                                                                                            >  1:185846/1‑245 (MQ=255)                                                                                                                                    gCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTa                                                                                                           <  1:83257/251‑1 (MQ=255)                                                                                                                                         aCTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTActgct                                                                                                      <  2:82085/251‑1 (MQ=255)                                                                                                                                             gaaaTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATTAATACCCACTACTTTACTGCTGATa                                                                                                  <  2:83925/250‑1 (MQ=255)                                                                                                                                              aaaTCGCCATGGTCGGCAGAGAGTGGTATGCGTTTCACAATGAGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATa                                                                                                  <  1:480836/250‑1 (MQ=255)                                                                                                                                                aTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAag                                                                                               <  1:69254/251‑1 (MQ=255)                                                                                                                                                aTCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTGCAGCGCCAGATCGTTGGGTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAag                                                                                               <  2:250128/251‑1 (MQ=255)                                                                                                                                                 tCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATaa                                                                                                 >  1:522636/1‑248 (MQ=255)                                                                                                                                                      aTGGTCGGCAGAGAGTTGTATGCGTTTCTCAATGCGACAACCAGGAAGTTTCAGCTCCTGATCGTTTGGTTCGTTACTCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAATGATTCGCTTAGTGGGAAGAGGTAGAGGGATTAAGGCCCACTATTTTACTGCTGATAAagaggagg                                                                                         <  2:382304/251‑5 (MQ=255)                                                                                                                                                      aTGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGagaagg                                                                                         <  1:56414/251‑2 (MQ=255)                                                                                                                                                        ggTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTGTACTTCTGATAAAGagaaggt                                                                                        <  2:377409/250‑3 (MQ=255)                                                                                                                                                         gTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGCGGCGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAACTCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTCGAGGGATGAATACCCACTAGTTTACTGCTGATAAaga                                                                                              >  2:279530/1‑243 (MQ=255)                                                                                                                                                         gTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAaga                                                                                              <  1:279530/243‑1 (MQ=255)                                                                                                                                                           cGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGaa                                                                                                                                         <  2:189140/198‑1 (MQ=255)                                                                                                                                                           cGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGaa                                                                                                                                         >  1:189140/1‑198 (MQ=255)                                                                                                                                                            ggCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAgg                                                                                  <  1:430755/251‑1 (MQ=255)                                                                                                                                                                gagagTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACg                                                                              >  1:590683/1‑251 (MQ=255)                                                                                                                                                                   agTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTaa                                                                           <  1:582369/251‑1 (MQ=255)                                                                                                                                                                         aTGCGTTTCTCAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTGCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATTCCCGCTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAAtctttt                                                                     <  1:601987/251‑1 (MQ=255)                                                                                                                                                                          tGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGCGTTTTTGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAACCGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTAATGCTGATAAAGAGA‑GAGTTCAGGTACGTAAtcttttt                                                                    >  1:35444/1‑250 (MQ=255)                                                                                                                                                                              tttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATcttttctttt                                                                >  2:137067/1‑251 (MQ=255)                                                                                                                                                                               ttCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATCTTTTCttttt                                                               >  2:298707/1‑251 (MQ=255)                                                                                                                                                                                tCACAATGCGCCAAACAGGAAGTTTGTGCACCAAATCGATGGCTTCGTTACGCAGCCATGCAATAATGACGAGGAGTTTATGGTCGGTTGCCTGCGCCGTGCAGAACAGCATCAGGCTAATCGCACGGCTGGCGGAACTCGTAACAACGGATTTACTAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAACGAGAAGG‑TTCAGGATCGTACTCTTTTCttttt                                                               >  2:587811/1‑250 (MQ=255)                                                                                                                                                                                   caATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGATAAGG‑TTCAGGTACGTAATCTTTTCTTTttatta                                                           <  2:110387/251‑1 (MQ=255)                                                                                                                                                                                       gCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATCTTTTCTTTTTATTACAAt                                                       <  2:19042/251‑1 (MQ=255)                                                                                                                                                                                             aaCAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTtg                                                 <  2:431747/251‑1 (MQ=255)                                                                                                                                                                                               cagtaaGTTTCAGCGCTATATCGTGTGTTACGTTACGCGGCATTGCAATGACACCTATGAGTTTATGGGCGTTTGCCTTCGCCGTGCAGCGCAGCATCAGGCTAATCGCCAGGCTGGCGGAAATAGTAAAAACGGATTTCATAAGGATTCTCTAAGTGGGAAGAGGTAGAGGGATGATTTCCCACTAGTTTACTGCTGATAAATAGAAGG‑TGCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTtgat                                               <  2:454584/247‑1 (MQ=255)                                                                                                                                                                                                             gcCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTg                                 >  1:591364/1‑251 (MQ=255)                                                                                                                                                                                                                 gATCGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGat                             <  2:177710/251‑1 (MQ=255)                                                                                                                                                                                                                    cGTTGGTTTCGTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGattca                          <  2:591364/251‑1 (MQ=255)                                                                                                                                                                                                                            tCGTTGCGCGGCAGTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCATAGCATCAGGCTAATCGCCAGTCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATGTTTTCTTTTTATTACGATTGTTTGATGAATGCCTTGGCTGCGAGTCATTCTTta                   <  2:390326/250‑1 (MQ=255)                                                                                                                                                                                                                              gTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTCATCTTTTCTTTTTCTTACAATTTTTTGATGAATGCCTTGGCTGCGattcatatatcgga                 >  2:78159/1‑242 (MQ=255)                                                                                                                                                                                                                              gTTACGCGGCATTGCAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGattcat                         <  1:78159/242‑1 (MQ=255)                                                                                                                                                                                                                                            cAATGACGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGAGGGATGAATACCCATTATTTTACTGCTGATAAAGAGAAGG‑TTCAGGTACGTAATCTTTTCTTTTTATTACAATTTTTTTATGAATGCCTTGGCTGCGATTCATTCTTTATCTGAATAAAATTGCTg  <  1:326101/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       GAGACGCAGCGTTTATTGTCGTTATCGCTGTTAATGTTGATCCAGTCAGTGGTTTGCCCTTCTTTTATTTCTGAAGGAATATTCAGGCTCTGACTGGCGCTACGGGCGGCTTTGAAATAAACCGATGCACCGCTTAACTGTAAATCGCCATGGTCGGCAGAGAGTTGTATGCGTTTCACAATGCGACAAACAGGAAGTTTCAGCGCCAGATCGTTGGTTTCGTTACGCGGCATTGCAATGGCGCCGAGGAGTTTATGGTCGTTTGCCTGCGCCGTGCAGCACAGCATCAGGCTAATCGCCAGGCTGGCGGAAATCGTAAAAACGGATTTCATAAGGATTCTCTTAGTGGGAAGAGGTAGGGGGATGAATACCCACTAGTTTACTGCTGATAAAGAGAAGA‑TTCAGGCACGTAATCTTTTCTTTTTATTACAATTTTTTGATGAATGCCTTGGCTGCGATTCATTCTTTATATGAATAAAATTGCTG  >  NC_000913/11455‑11940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑