Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,323	C→T	V329V (GTC→GTT)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,323	0	C	T	99.0%	310.0 / NA	98	V329V (GTC→GTT)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (56/40);  minor base G (0/1);  total (56/42)
Fisher's exact test for biased strand distribution p-value = 4.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑ACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGG  >  NC_000913/1080‑1568                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       gATGTCCTACCAGGAAGCGATGGCGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATgg                                                                                                                                                                                                                                                 >  1:149596/1‑251 (MQ=255)        tACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGgcgcg                                                                                                                                                                                                                                          >  2:243403/1‑251 (MQ=255)         aCCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGgcgcgc                                                                                                                                                                                                                                         >  2:121099/1‑251 (MQ=255)            aGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGtg                                                                                                                                                                                                                                      >  1:519283/1‑250 (MQ=255)                aGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTg                                                                                                                                                                                                                                  >  1:231809/1‑251 (MQ=255)                    attgAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTGCCAGATCCCTTGCCTGATTAAAAAGTCCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGTTGCCAGCCGTTATGAAGACGAATTACCGGTCAAGGGCAGTTCCAATCTGAATAATATGGCAAGGTTCTGCGTTTCCTGCCCGGGTATTAAAGGGATGGGTGGCATGGCGgcgcgctggtttgcagc                                                                                                                                                                                                                              <  2:412174/248‑12 (MQ=255)                    aTGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCCTTCACCCC‑ATCGCCCAGTTCCAGATCACTTGACTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACACTCATTGGTGCCAGCCGTGATGAAGACGACTTACCGGCCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGGGTGTTTGCAGc                                                                                                                                                                                                                              >  1:296005/1‑251 (MQ=255)                     tGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TACAGGTACGATCATTGGTGCCAGCCGTGATGAAGACGAATTACAGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCg                                                                                                                                                                                                                             >  1:75706/1‑251 (MQ=255)                      ggAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATCACACCC‑ATCGCCCAGTTCCAAACCCCTTGCCTGATTAAAAATAACGCCAATCCTCAAGCTACC‑GGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAACGGCATTTCCAATCTGAAATATATGGCAATGTTCAGCGTTTCCGGCCCGGGGCTGAAAGGGATGGTTGGCATGGCGTCGCGCGGCTTTGCAGCg                                                                                                                                                                                                                             >  1:581603/1‑250 (MQ=255)                           tttCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGgtgta                                                                                                                                                                                                                        >  2:483405/1‑249 (MQ=255)                           tttCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTc                                                                                                                                                                                                                        >  2:357042/1‑250 (MQ=255)                            ttCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCa                                                                                                                                                                                                                       <  1:357042/250‑1 (MQ=255)                                  cTTCGGCGCTAAAGTTATTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGACAGGGATGGTTGGCATGGCGGCGCGGGTGTTTGCAGCGATGTCACGCGccc                                                                                                                                                                                                                >  1:515480/1‑251 (MQ=255)                                    tCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTTCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGccc                                                                                                                                                                                                                <  1:582031/250‑1 (MQ=255)                                       gcgcTAAAGTTCTTCACCCCCGCACCATTACCCCCCATCGCCCAGTTCCAGATCCCTTGCGTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTTGTGCCAGCCGTGATCAAGTCGAATTACCGGTCAAGGGCATTTCCTATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGTTGATGAAATGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCTCGt                                                                                                                                                                                                              <  1:402833/249‑1 (MQ=255)                                         gcTAAAGTTCTTCCCCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTc                                                                                                                                                                                                         <  1:591227/251‑1 (MQ=255)                                               gTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCggggg                                                                                                                                                                                                   >  1:172979/1‑247 (MQ=255)                                                    tcACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATGCCGGAAATCCTCAAGC‑TCCAGTTACGCTCATTGTTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGGATCCGGCCCGGGGATGAGAGGGATGGTTGGCATGTCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCt                                                                                                                                                                                               <  1:359262/249‑1 (MQ=255)                                                    tcACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGAGCGCGTGTTTGCAGCGCAGTCACAAGCCCGTATTTCCG‑GGGTGCttg                                                                                                                                                                                             >  1:53861/1‑249 (MQ=255)                                                    tcACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTAAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCt                                                                                                                                                                                               <  1:352360/249‑1 (MQ=255)                                                     cACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGCTTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTGTGCGGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGAt                                                                                                                                                                                            >  2:189294/1‑251 (MQ=255)                                                      ccccccGCACCATTACCCCC‑ATCGCCCAGTGCCAGATCCCGTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATGACCGGTCAAGGGCATTTCCAATCTGAATAATATGTCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGTTTGTCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGAtt                                                                                                                                                                                           <  2:123938/250‑1 (MQ=255)                                                           cGCACCATTACCCCC‑ATCGCCCCGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGGATTTCCG‑TGGTGCTGATTACGc                                                                                                                                                                                       >  1:161638/1‑250 (MQ=255)                                                              aCCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGGTTACGCAatc                                                                                                                                                                                   >  1:384469/1‑251 (MQ=255)                                                              aCCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatc                                                                                                                                                                                   <  1:243403/251‑1 (MQ=255)                                                               ccATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGTTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatca                                                                                                                                                                                  <  1:23007/251‑1 (MQ=255)                                                                cATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatcat                                                                                                                                                                                 >  1:65395/1‑251 (MQ=255)                                                                 aTTACCCCC‑AGCGCCCAGTTCCAGATCCATTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatcatc                                                                                                                                                                                <  1:608666/251‑1 (MQ=255)                                                                   tACCCCC‑CTCGCCCAGTTCCAGATCCCTTGCATGATTACAAATACCGGAAATCCTCAAGC‑TCCAGGTCCGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGGTGTCCCGCGCCCGCATTTCCG‑TGGTGCTGATTTCGCaagc                                                                                                                                                                                   >  2:102503/1‑244 (MQ=255)                                                                   tACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAatc                                                                                                                                                                                   <  1:102503/246‑1 (MQ=255)                                                                     tcccacagcGCCCAGTTCCATGTCCCTCGCCTGACTACAAAGTCCGGACAACCTCAAGC‑TCCAGCTGCGCTCTTTGGTGCCAGCCGTGATTCAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATTATATAGGAATGTACAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCTGAGCGCGTGTTTGCAGCGATGTGACGCGCCCGTATGTCCG‑TGGTGCGGATTCCGCAATCATCtt                                                                                                                                                                              <  2:337812/242‑1 (MQ=255)                                                                      cccccacagCCCAGTTCCAGATCCCTTGCCGGATTAAAAATCCCTGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTTCCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGTATGAAAGGGATGGTTGGTATGGCGGCGCACGGGGTTGCAGCGATGTCTCGCGCCCGTTTTTGCGATGG‑GCTGATTACGctgtcagcttcc                                                                                                                                                                            <  2:603549/243‑12 (MQ=255)                                                                                ccAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCGTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCTGTCAAGGTCATTTCCAATCTGAATAATATGGCAATGTGCAGCTTTTCCGGCCCGGGGATTAAAGGGATGGTAGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGGATTCATCTTCCTAATACatta                                                                                                                                                                  <  2:35527/251‑4 (MQ=255)                                                                                ccAGTACCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCGCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTGCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCTCGCGCCCGTATATCCG‑TGGGGCTGATTACGCAATCATCTTCCGAATACAgta                                                                                                                                                                  <  2:60219/251‑3 (MQ=255)                                                                                       cAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAACACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGttt                                                                                                                                                           >  2:414465/1‑251 (MQ=255)                                                                                        aGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCCGCGCTGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTc                                                                                                                                                          >  1:38696/1‑251 (MQ=255)                                                                                         gATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCt                                                                                                                                                         <  2:161638/251‑1 (MQ=255)                                                                                               ttGCCTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGGGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTc                                                                                                                                                   >  1:561776/1‑251 (MQ=255)                                                                                                 gCCTGATTAAAAATACCGGAACTCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGGATACAGTATCAGTTTCTGCGTTccg                                                                                                                                                 >  2:110636/1‑250 (MQ=255)                                                                                                   cTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGCTGTCACGCGCCCGGATTTCCG‑TGGTGCTGGTTACGCAATCATCTTCCGCATACAGTATCAGTTTCTGCGTTccgc                                                                                                                                                >  2:472822/1‑248 (MQ=255)                                                                                                   cTGATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGGTTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTccgc                                                                                                                                                >  2:175262/1‑248 (MQ=255)                                                                                                     gATTAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGTCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCaaa                                                                                                                                             <  1:323895/251‑1 (MQ=255)                                                                                                        tAAAAATACCGGAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGc                                                                                                                                           <  1:110636/250‑1 (MQ=255)                                                                                                         aaaaaTACCGGAAATCCCCAAGC‑TCCATGTTCGCGCATTGGTGCCAGCCGGGATGAAGACGAAGTACCGGGCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCGGATTACGCAATCATCTTCCGAATACAGTAGCAGTTTCTGCGTTCCGCAAAGCg                                                                                                                                          <  2:381568/250‑1 (MQ=255)                                                                                                               aCCGGAAATTCTCAAGC‑TCCAGGTACGCGCATTGGTGCCAGCCGTGAAGAAGACGCATTGCCGGTCAAGGGCATTTCCAATCTGAACACTAGGGCAATGTTCAGCGTTTCCGGCCCGGGGAAGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTTCG‑TGGTGCTGATTACGCAATCATCTTCCGAATCCAGTATCAGTTTCTGCGTCCCGCAAAGCGACtgtgt                                                                                                                                   <  2:533448/251‑1 (MQ=255)                                                                                                                 cgtaaaaccTCAAGC‑TCCAGGTACGCGCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAAGACAGTATCATTTTCTGCGTTCAGCAAAGCGACTGTGTGc                                                                                                                                 <  2:87690/244‑1 (MQ=255)                                                                                                                  ggAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCg                                                                                                                                >  1:434441/1‑251 (MQ=255)                                                                                                                   gAAATCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCACTCTGAATAATATGGCACTGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGAGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGCATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCgg                                                                                                                               >  1:377384/1‑250 (MQ=255)                                                                                                                    aaaTCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAg                                                                                                                              <  1:414465/251‑1 (MQ=255)                                                                                                                     aaTCCTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGTTGAAAGGGATGGTTGGCATGGCGCCGCGCGTGTTTGCAGAGATGTCACGCGCCCGTATTTCAG‑TGGTGCTGATTACGCAATCATCTTCCGAATCCAGTATCAGTTTCTGCGTTCCGCAAAGCGTCTGTGTGCGAGc                                                                                                                             >  1:382787/1‑251 (MQ=255)                                                                                                                        ccTCAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTTATGAAGACGAAGTACCGGTCAAGGTCATTGCCAATCTGAATAAGAGGGCAATGTTCAGCTTTTCCGGCCCGGGGATGAAAGGGGTGGTTGGCATGGCGGCTCGCGTGTTTGCAGCGATGTCACGCGCCTGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACATTATCAGTTGCTGCGTTCCGCAAAGCGACTGTGTTCGAGCTGa                                                                                                                          <  2:296005/251‑1 (MQ=255)                                                                                                                          ccAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGGGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAAc                                                                                                                        <  2:129594/250‑1 (MQ=255)                                                                                                                           cAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCCGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACg                                                                                                                       >  2:132323/1‑251 (MQ=255)                                                                                                                           cAAGC‑TCCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACg                                                                                                                       >  1:557567/1‑251 (MQ=255)                                                                                                                                  ccAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCaa                                                                                                                  <  1:253078/250‑1 (MQ=255)                                                                                                                                  ccAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGAGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAAt                                                                                                                 >  1:396849/1‑251 (MQ=255)                                                                                                                                   cAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATg                                                                                                                >  1:592189/1‑251 (MQ=255)                                                                                                                                      gTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCACGGGCATTTCCAATCTGAATAATATGGCAATGTTCCGCGTTTCCGGCCTGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCCCGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGGATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGc                                                                                                               >  1:619097/1‑249 (MQ=255)                                                                                                                                       gaCGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGGCAAGGGCATGTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAgg                                                                                                            <  2:515480/250‑1 (MQ=255)                                                                                                                                         cGCTCATTGGAGCCAGCCGTGATGAAGACGAATTACCGGACAAGGGCATTTCCAATCTGGATAATATGGAAATGTTCAGCGTTTCCGGCACGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGAGTGTTTGCCGCGCTGTCACGGGCCCGTATTTCCG‑GGGGGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCACAACGACTTGGCGCGAGCGGAACGGGCAATGCAggg                                                                                                           >  2:621644/1‑249 (MQ=255)                                                                                                                                              aTTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGtt                                                                                                     <  1:132323/251‑1 (MQ=255)                                                                                                                                                tGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTc                                                                                                    <  1:542522/250‑1 (MQ=255)                                                                                                                                                 ggTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCt                                                                                                   <  1:189294/250‑1 (MQ=255)                                                                                                                                                    gacAGCCGTGATGAAGACTAATTACCTGTCAAGGGCAGTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACGGTGTGCGAGCTGAACGGGCAATGCAGGAATAGTTCTAcc                                                                                                <  2:377384/248‑1 (MQ=255)                                                                                                                                                     ccAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATAGGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAAGGCAGGAAGAGTTCTACCTg                                                                                              <  2:400791/251‑1 (MQ=255)                                                                                                                                                       aGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGAGGTTTTGTAGCGATGGCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTACGAATACCGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGCAGAGTTCTACCttgg                                                                                            >  1:573853/1‑248 (MQ=255)                                                                                                                                                            tgatgaAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGGGTTTGCAGCGCTGTCACGCGCCCGTATTTCCG‑TGGGGCTGATTACGCAATCCTCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGATCTACCTGGAACTg                                                                                        >  2:185186/1‑250 (MQ=255)                                                                                                                                                                 aaGACGAATTACCGGTCAAGGGCAGTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTACGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAaagaa                                                                                  <  2:401480/251‑1 (MQ=255)                                                                                                                                                                      gAATTACCGGTCAAGGGCATTTCAAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCCGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGACCTGGAAGAAGGCtt                                                                             >  2:35643/1‑251 (MQ=255)                                                                                                                                                                       aaTTACCGGTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCCATGCAGGAAGAGTTCTACCTGGGACTGAAAGAAGGCtt                                                                             >  2:215259/1‑250 (MQ=255)                                                                                                                                                                         ttACCGGTCAAGGGCATTTCCACTCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACt                                                                          >  1:496727/1‑251 (MQ=255)                                                                                                                                                                              ggTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGc                                                                     <  2:519283/251‑1 (MQ=255)                                                                                                                                                                              ggTCAAGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGc                                                                     >  1:311910/1‑251 (MQ=255)                                                                                                                                                                                  aaGGGCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCt                                                                 >  2:234876/1‑251 (MQ=255)                                                                                                                                                                                    gggCATTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGGGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTGACTGGAGCCGCTgg                                                               >  2:482308/1‑251 (MQ=255)                                                                                                                                                                                        aTTTCCAATCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGcggg                                                           >  1:271458/1‑248 (MQ=255)                                                                                                                                                                                              aaTCTGAATAATATGGCAATGTTCAGCGTTTCTGGCCCGGGGCTGACAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGCTGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCCATCCTCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATg                                                                                                                >  2:112717/1‑192 (MQ=255)                                                                                                                                                                                              aaTCTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATg                                                                                                                <  1:112717/192‑1 (MQ=255)                                                                                                                                                                                                 cTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGTCTTACTGGAGCCGCTGGCGGTGACGGGACg                                                  >  2:224192/1‑251 (MQ=255)                                                                                                                                                                                                     ataataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGGGACGGAACGGCTg                                              >  1:267049/1‑251 (MQ=255)                                                                                                                                                                                                               aaTGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGGTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGCGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGGCGGAAAGGCGGGCCCTTAtct                                    >  2:294235/1‑251 (MQ=255)                                                                                                                                                                                                                 tGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCg                                  >  1:19880/1‑251 (MQ=255)                                                                                                                                                                                                                     cAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGAATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGCAACCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGCAAGAAGGCTTAATGGAGACGCTGGCGGCGACCGGACGGCTGGcccatttctcggggg                              >  1:572932/1‑238 (MQ=255)                                                                                                                                                                                                                        cGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGAGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCggtggt                             >  1:576449/1‑249 (MQ=255)                                                                                                                                                                                                                        cGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGAGCGCGTGTTTGCAGCGATGTCACGCGCCCGTCTTTCCG‑TGGCGCTGATTACGCCATCATCTTCCGAATCCAGTATCCGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTCCTGGAGCCGCTGGCGGGGACGGAACGGCTGGCCATTCTCTCgggggt                             >  1:562241/1‑249 (MQ=255)                                                                                                                                                                                                                           ttccggcccggtggtGAAAGGGATGGTTGGCATGGCGGCGCGCCTGGTTGCAGCGATGTCACGCGCCCGTCTTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGATTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCGGAACGGGCAATGCTGGGAGAGTTCTACATGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGAAGGAAAGGCTGCCCAATATATCgggtggagggg                        >  2:54758/15‑242 (MQ=255)                                                                                                                                                                                                                               ggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGAGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCACTCATCTTCCGAATACAGTATCCGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCCGGAAGAGTTCTACCTGGAACTGAAATAAGGCTTACTGGAGCCGCTGGCGGTGACCGCACTGCTGGCCACTATCTCGGTGGCAGGGGATgg                    >  1:12759/4‑251 (MQ=255)                                                                                                                                                                                                                                gcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGGGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCCTCTTCCGAATACAGGATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCACTGCCGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCTGTGGCGGAACGGCCAGCCCATATCTCggtgggtggggaaggg                   >  2:60404/3‑240 (MQ=255)                                                                                                                                                                                                                                 cccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTa                  <  1:218988/250‑1 (MQ=255)                                                                                                                                                                                                                                 cccGGGGATGAAAGGGATGGTTGGCAGGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTGCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTAGCTCGGTGGTAGGGGATGGTa                  <  2:592189/250‑1 (MQ=255)                                                                                                                                                                                                                                     gggATGAAAGGGATGGTGGGCATGGCGGCGCGCGTGTTTGCAGCGAGGTCACGCGCTCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTGCTGCCAGGAACTGAAAGAAGGCGTACTGGAGGGGCTGGCGGTGACGGAACGGCGGGCCATTAGCTCGGTGGTAGGGGATGGTATgcg              <  2:34342/251‑1 (MQ=255)                                                                                                                                                                                                                                      ggATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCCTTATCTCGGTGGTAGGTGGTGGTATgcgc             >  1:334771/1‑251 (MQ=255)                                                                                                                                                                                                                                        aTGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTGGCAGCGATGTCACGTGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCGGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCAc           <  1:596459/251‑1 (MQ=255)                                                                                                                                                                                                                                          gAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCAcc          <  1:56547/250‑1 (MQ=255)                                                                                                                                                                                                                                            aaGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCCTTAtc                                     >  1:221702/1‑221 (MQ=255)                                                                                                                                                                                                                                            aaGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTAtc                                     <  2:221702/221‑1 (MQ=255)                                                                                                                                                                                                                                            aaGGGATGGTTGGCATGGCGGCCCCCGTGTTTGCAGCGCTGTCACGCGCCCGTCTTTCCG‑TGGGGCTGATTACGCAATCATCTTCCGACAACAGTATAAGTTTCTGCGTTCCGCAAAGCGACTGTGCGCAAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGCACTGAAAGAAGGCTTACTGGAGCCGCGGGCGGTGAAGGAACGGGTGGGCATTATTTCGGTGGGAgggggagggatgtgcaccctt       >  2:115366/1‑232 (MQ=255)                                                                                                                                                                                                                                                 aTGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCGGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTgg  <  1:340212/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       GATGTCCTACCAGGAAGCGATGGAGCTTTCCTACTTCGGCGCTAAAGTTCTTCACCCCCGCACCATTACCCCC‑ATCGCCCAGTTCCAGATCCCTTGCCTGATTAAAAATACCGGAAATCCTCAAGC‑ACCAGGTACGCTCATTGGTGCCAGCCGTGATGAAGACGAATTACCGGTCAAGGGCATTTCCAATCTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCG‑TGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGCTGGCAGTGACGGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGG  >  NC_000913/1080‑1568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑