Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,544	A→G	Q201Q (CAA→CAG)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,544	0	A	G	100.0%	106.1 / NA	33	Q201Q (CAA→CAG)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/18);  total (15/18)

ACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4641299‑4641652                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     aCTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACg                                                                                                                                                                                                                                                 <  1:448577/251‑1 (MQ=255)    aaCGAAGAGTTAGCGGTGGCTGACGTTCTTACGGGGGTGCTGATGGTGGCAGATTATACTGCGCTCAATCTGGCGCAGGCAGTGATGGGCTAGTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATGGCTGACGACTCTGGCAGTGGCAGTTGACATAAAACTGGTCGACTGGTTACAGCAACGCCt                                                                                                                                                                                                                                              <  2:105790/251‑1 (MQ=255)              tAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGAGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGCCATAAAACTGGTCGCCTGGTTACAGCAACGCCTGGGGCtttt                                                                                                                                                                                                                                     >  2:538925/1‑250 (MQ=255)                     ggCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCaa                                                                                                                                                                                                                              <  2:209162/250‑1 (MQ=255)                           cGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGaca                                                                                                                                                                                                                       <  1:390702/251‑1 (MQ=255)                              tCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACgg                                                                                                                                                                                                                    >  1:163746/1‑251 (MQ=255)                              tCTTACTGGAGTGCCGATCGAGGCAGATTATCCTTCGCTCCATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGAGACATTAATAAAGCAACCGACGAAAAGTGACCCAACGTCAGACCAACATCAACTGCACGCTTTACGCGAACGAGTCATGGCATTGCTGCCGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACgg                                                                                                                                                                                                                    >  2:278939/1‑251 (MQ=255)                                     ggTGTGCCGATGGTGGCATATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGtt                                                                                                                                                                                                             <  1:610400/251‑1 (MQ=255)                                           ccGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCg                                                                                                                                                                                                       <  1:437795/251‑1 (MQ=255)                                                ggtggCAGATTATCCTTCGCTAAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTtgc                                                                                                                                                                                                  >  1:141822/1‑251 (MQ=255)                                                                 cGCTCAAGTTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGGCATGGCAGTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGaaaa                                                                                                                                                                                  <  2:458450/250‑1 (MQ=255)                                                                     cAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAata                                                                                                                                                                             <  1:521635/251‑1 (MQ=255)                                                                          tGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCAcc                                                                                                                                                                        >  2:485060/1‑251 (MQ=255)                                                                           ggggCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATc                                                                                                                                                                           <  1:602121/247‑1 (MQ=255)                                                                                 ggcagTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATaaa                                                                                                                                                                 <  2:565909/247‑1 (MQ=255)                                                                                     gTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATAACCAAATAAAAAAc                                                                                                                                                             >  2:262852/1‑251 (MQ=255)                                                                                            tCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCtt                                                                                                                                                        >  2:329358/1‑249 (MQ=255)                                                                                                            aGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagct                                                                                                                                      >  1:566946‑M2/1‑247 (MQ=255)                                                                                                                      aTACAACAACCGCCGAAAAGTGACACAACGGCAGACCAGCATCAAATGAAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTCGTAAGTATTTTTCagcttttcattctg                                                                                                                            <  2:66583‑M2/251‑15 (MQ=255)                                                                                                                      aTACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctg                                                                                                                            <  1:470514‑M2/251‑15 (MQ=255)                                                                                                                             aacCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAACCGCCTTAGTAAGTATTTTTCagcttttcattctgactgca                                                                                                                      <  1:146728‑M2/250‑21 (MQ=255)                                                                                                                                cgacgaaAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgg                                                                                                                  <  1:414523‑M2/248‑25 (MQ=255)                                                                                                                                      aaaGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaata                                                                                                            >  2:163746‑M2/1‑221 (MQ=255)                                                                                                                                           gacacaacgGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGCTGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGAAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtct                                                                                                       <  2:469334‑M2/247‑36 (MQ=255)                                                                                                                                                   ggCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtgg                                                                                               >  1:329358‑M2/1‑208 (MQ=255)                                                                                                                                                     cAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggat                                                                                             <  1:328776‑M2/251‑46 (MQ=255)                                                                                                                                                      aGACCAACATCAACTGCAAGCGTTACGCTAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACAGAAAACTGGTCGACTGGTTACAGCAACGCCTTGGTCTTGTAGAGCAACGAGACACGGCAATGTTGCACTGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTATTAAGTATTTTTCagcttttcagtcggactgcaacgggcaatatgtctcggtgaggatt                                                                                            >  2:605152‑M2/1‑205 (MQ=255)                                                                                                                                                                       aaGCTTTACGCGAACGAGTCATGGCATTGATGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGACAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctg                                                                            <  1:207376‑M2/250‑63 (MQ=255)                                                                                                                                                                                            tggcattGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggtt                                                       <  1:142671‑M2/247‑84 (MQ=255)                                                                                                                                                                                             ggcatTGCTGACGAGTCTGGCAGTGGCAGATGACATAAAACTGGTCGGCTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCAACGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcatttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttac                                                     >  2:196295‑M2/3‑166 (MQ=255)                                                                                                                                                                                                aTTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctg                                                  >  2:142671‑M2/1‑163 (MQ=255)                                                                                                                                                                                                                     ggcagATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtgagtaaattaaaatttt                             >  1:542980‑M2/1‑142 (MQ=255)                                                                                                                                                                                                                                                gTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTCagcttttcattctgactgcaacgggcaatatgtctctgtgtggattaaaaaaagagtgtctgatagcagcttctgaactggttacctgccgtgagtaaattaaaattttattgacttaggtcactaaatactttaa  >  2:414523‑M2/1‑115 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGCTGCATGATATTGAAAAAAATATCACCAAATAAAAAACGCCTTAGTAAGTATTTTTC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4641299‑4641652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑