Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,352	G→A	A137A (GCG→GCA)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,352	0	G	A	100.0%	182.7 / NA	59	A137A (GCG→GCA)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/35);  total (24/35)

TGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGC  >  NC_000913/4641106‑4641596                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     tGATAATATTAACGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGTTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGAtt                                                                                                                                                                                                                                                  >  2:521635/1‑251 (MQ=255) tGATAATATTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGAt                                                                                                                                                                                                                                                   <  1:141697/250‑1 (MQ=255) tGATAATATTAAAGTTTGCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACGGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGAt                                                                                                                                                                                                                                                   <  2:386632/250‑1 (MQ=255)       tatTAAAGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCtt                                                                                                                                                                                                                                            >  1:565909/1‑251 (MQ=255)             aGTTTTCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCAATGTCGCCACCACTGCGCGCAGTCGGGCGAAATCTCATTACTACGCTAAGCAAATAGAACTGGTACCGCTGTTAGCGGAAAAATCTTCATGGATGAGCCCTGAAGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACAGGTGTGTCGATGGTGGCAGATTATACATCGCTc                                                                                                                                                                                                                                       >  1:371368/1‑250 (MQ=255)                  tCCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCt                                                                                                                                                                                                                                  <  2:551329/250‑1 (MQ=255)                      gACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGc                                                                                                                                                                                                                             <  1:163965/251‑1 (MQ=255)                         actGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAgg                                                                                                                                                                                                                          >  1:10635/3‑251 (MQ=255)                               tGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTg                                                                                                                                                                                                                     >  1:114729/1‑250 (MQ=255)                                  aTCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATgg                                                                                                                                                                                                                 <  2:537279/251‑1 (MQ=255)                                    cGTTACACGATGTCGATTTCACTGTCGCCACCACGTCGCGCAGTCGGGCGAAATATCATTACGACGTTACGCCAGGAGAACTGGTACCGCTGTGATAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATGCCGGGTGGAGTAACGAAGAGTTAGCGTGGGCTGACGTGCTTACTGGGGTGGCGAGGGGGGAAGATTATCCGTCGCTCAATTTGGTGCAGGCAGCGATGGt                                                                                                                                                                                                                <  2:403553/250‑1 (MQ=255)                                     gTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCt                                                                                                                                                                                                              <  1:174797/251‑1 (MQ=255)                                      ttACACGATGCCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCt                                                                                                                                                                                                              >  1:196295/1‑250 (MQ=255)                                       tACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTa                                                                                                                                                                                                             <  2:403144/250‑1 (MQ=255)                                                 cGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCGGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTACCGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAAt                                                                                                                                                                                                  >  2:610400/1‑251 (MQ=255)                                                  gATTTCACGGTCGCCACCAATGCGCGCAGTCGGGTGAAATATCATTACTACGCTACGCCAGTAGAATTGGTACCGCTGTTAGAGGAAAAATCTTCAGGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGTGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATTCTTCGCTCAATCTGGGGCAGGCAATGATGGGCGATGGCTATCAAtg                                                                                                                                                                                                 <  2:390440/251‑2 (MQ=255)                                                       cacggtcgactccacTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGGTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGGCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCTGATGGTGGCAGATTCTCTTTCGCTCAATCTGGGGCAGGCGGTGATGGGCTATTGCGATCAATTAGCaa                                                                                                                                                                                            <  2:110734/240‑1 (MQ=255)                                                                    acTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATacaaca                                                                                                                                                                               <  1:162424/251‑1 (MQ=255)                                                                          cgcAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCgac                                                                                                                                                                         <  1:3815/251‑3 (MQ=255)                                                                          cgaaGTCGGGAGAAATATCATTAATACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGATGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTATGCCGATGGTGGCAGATTATCCTTCGCTCAATCTAGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCACCATTAATACAACAACCgac                                                                                                                                                                         >  2:554643/4‑249 (MQ=255)                                                                                    cGAAATATCATTACTACGCTACGCCAGTAGAACTGGTCCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCAGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAACGTGac                                                                                                                                                               >  2:566946/1‑250 (MQ=255)                                                                                        atatCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGacacaa                                                                                                                                                           <  2:438484/251‑6 (MQ=255)                                                                                          atCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGacacaac                                                                                                                                                          <  1:560898/250‑7 (MQ=255)                                                                                          atCATTAATACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACAGACGAAAAGTGacacaacg                                                                                                                                                         >  2:448577/1‑244 (MQ=255)                                                                                                                 gAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGc                                                                                                                                  <  1:562557/251‑1 (MQ=255)                                                                                                                  aaCTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTg                                                                                                                                                                                                          <  1:410061/178‑1 (MQ=255)                                                                                                                  aaCTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTg                                                                                                                                                                                                          >  2:410061/1‑178 (MQ=255)                                                                                                                            gCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTg                                                                                                                                                                                                                     >  2:482110/1‑157 (MQ=255)                                                                                                                            gCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTg                                                                                                                                                                                                                     <  1:482110/157‑1 (MQ=255)                                                                                                                               gTTAGAGTAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGCCCCAACGGCAGCCCAACATCAACTGCAAGCTTTACGCGAAcc                                                                                                                      >  2:26681/1‑248 (MQ=255)                                                                                                                                   gagGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCAt                                                                                                                <  1:129547/251‑1 (MQ=255)                                                                                                                                         aaaTCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCAtt                                                                                                          <  1:103095/251‑1 (MQ=255)                                                                                                                                               tCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTga                                                                                                     <  1:297865/250‑1 (MQ=255)                                                                                                                                               tCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGGCATGGCATTGc                                                                                                        <  1:130806/247‑1 (MQ=255)                                                                                                                                                   ggatgcaccATGCCGGGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGTCTAACGAAGAGTTAGCGTTGGCTGACGTTCGTACTGGTGTGCCGATTGTGGCAGATTATCCTTCGCACAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCTTTGCTgacgac                                                                                                 <  1:446090/243‑1 (MQ=255)                                                                                                                                                      tGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTAGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAAAAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCAGGGCATTGCTGACGACTCtg                                                                                             >  2:482207/1‑251 (MQ=255)                                                                                                                                                       gAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGCTTCCGGGCTGCCTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGCTTATCCTTCGCTCAATCTGGGGCa                                                                                                                                                                                                                            >  2:228466/1‑123 (MQ=255)                                                                                                                                                       gAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTGCGCTCAATCTGGGGCa                                                                                                                                                                                                                            <  1:228466/123‑1 (MQ=255)                                                                                                                                                         gCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCtggca                                                                                          <  2:335605/251‑1 (MQ=255)                                                                                                                                                                   gcTGGTGTTTGGTCGAGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGATCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATg                                                                                >  1:446625/1‑251 (MQ=255)                                                                                                                                                                      ggTGTTTGGTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGTGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACa                                                                             <  2:148497/251‑1 (MQ=255)                                                                                                                                                                              gTCGCGAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTg                                                                     <  2:613308/251‑1 (MQ=255)                                                                                                                                                                                cgcgAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGt                                                                   >  1:394193/1‑251 (MQ=255)                                                                                                                                                                                   gAAGATTCCGGGCTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCACTAAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTCGCAACATTAATACAACAACAGCCGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTCAGCGAACGAGTCATGGCATTGCTGACGAATCTGGCAGTGGCAGATGACATAAAACTGGTCg                                                                 >  1:605152/1‑250 (MQ=255)                                                                                                                                                                                     aGATTCCGGGCTGACGAACGAAGGGTTGGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACt                                                              <  1:334330/251‑1 (MQ=255)                                                                                                                                                                                                  aCTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACg                                                 <  1:448577/251‑1 (MQ=255)                                                                                                                                                                                                     aaCGAAGAGTTAGCGGTGGCTGACGTTCTTACGGGGGTGCTGATGGTGGCAGATTATACTGCGCTCAATCTGGCGCAGGCAGTGATGGGCTAGTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATGGCTGACGACTCTGGCAGTGGCAGTTGACATAAAACTGGTCGACTGGTTACAGCAACGCCt                                              <  2:105790/251‑1 (MQ=255)                                                                                                                                                                                                      aCGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCCTTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGtt                                                          >  2:540113/1‑238 (MQ=255)                                                                                                                                                                                                      aCGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGtt                                                          <  1:540113/238‑1 (MQ=255)                                                                                                                                                                                                               tAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGAGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGCCATAAAACTGGTCGCCTGGTTACAGCAACGCCTGGGGCtttt                                     >  2:538925/1‑250 (MQ=255)                                                                                                                                                                                                                      ggCTGACGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCaa                              <  2:209162/250‑1 (MQ=255)                                                                                                                                                                                                                            cGTTCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGaca                       <  1:390702/251‑1 (MQ=255)                                                                                                                                                                                                                               tCTTACTGGTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACgg                    >  1:163746/1‑251 (MQ=255)                                                                                                                                                                                                                               tCTTACTGGAGTGCCGATCGAGGCAGATTATCCTTCGCTCCATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGAGACATTAATAAAGCAACCGACGAAAAGTGACCCAACGTCAGACCAACATCAACTGCACGCTTTACGCGAACGAGTCATGGCATTGCTGCCGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACgg                    >  2:278939/1‑251 (MQ=255)                                                                                                                                                                                                                                      ggTGTGCCGATGGTGGCATATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGtt             <  1:610400/251‑1 (MQ=255)                                                                                                                                                                                                                                      ggTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAgtc                                                                                                                  >  1:56096/1‑148 (MQ=255)                                                                                                                                                                                                                                      ggTGTGCCGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAgtc                                                                                                                  <  2:56096/150‑3 (MQ=255)                                                                                                                                                                                                                                            ccGATGGTGGCAGATTATCCTTCGCTCAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCg       <  1:437795/251‑1 (MQ=255)                                                                                                                                                                                                                                                 ggtggCAGATTATCCTTCGCTAAATCTGGGGCAGGCAGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGACGAAAAGTGACACAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGTCATGGCATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAGCAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTtgc  >  1:141822/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     TGATAATATTAAAGTTTTCCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTTGGTCGCGAAGATTCCGGGTTGACTAACGAAGAGTTAGCGTTGGCTGACGTTCTTACTGGTGTGCCGATGGTGGCGGATTATCCTTCGCTCAATCTGGGGCAGGCGGTGATGGTCTATTGCTATCAATTAGCAACATTAATACAACAACCGGCGAAAAGTGATGCAACGGCAGACCAACATCAACTGCAAGCTTTACGCGAACGAGCCATGACATTGCTGACGACTCTGGCAGTGGCAGATGACATAAAACTGGTCGACTGGTTACAACAACGCCTGGGGCTTTTAGAGCAACGAGACACGGCAATGTTGCACCGTTTGC  >  NC_000913/4641106‑4641596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑