Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,939	G→A	F7F (TTC→TTT)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,939	0	G	A	100.0%	197.5 / NA	60	F7F (TTC→TTT)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/38);  total (22/38)

GTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCA  >  NC_000913/7695‑8183                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       gTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGgag                                                                                                                                                                                                                                                  <  1:450849/251‑1 (MQ=255)        ggcggTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGCAATGAGGTTAAACCGCCTGGCTGTGGATGCATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGCAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGACGAGCAGGTAAATCATTACCGCTCCCCAAAGGACGCTGTTAATAAAGGAGACAAAAt                                                                                                                                                                                                                                           >  1:564457/1‑251 (MQ=255)               attgCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGgcat                                                                                                                                                                                                                                    <  1:260682/248‑1 (MQ=255)               attgCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGgcat                                                                                                                                                                                                                                    <  1:53236/248‑1 (MQ=255)               attgCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGgcat                                                                                                                                                                                                                                    <  1:350145/248‑1 (MQ=255)                     aGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGTTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCatat                                                                                                                                                                                                                              <  1:340817/251‑1 (MQ=255)                         cAACGCCGGCCAGGTTGCCGCTAACCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGAGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCct                                                                                                                                                                                                                          >  2:456211/1‑251 (MQ=255)                                   cAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTAAACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCg                                                                                                                                                                                                                <  2:43479/251‑1 (MQ=255)                                               aCCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACt                                                                                                                                                                                                    >  2:83412/1‑251 (MQ=255)                                                        cgccgcAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGGGAAAAAATTTGGCATGCATATCCCTCTTATTGCAGGTCGAGATGGCTTTCCTgtgt                                                                                                                                                                                           >  2:233560/1‑251 (MQ=255)                                                               aGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGtt                                                                                                                                                                                    <  1:484345/251‑1 (MQ=255)                                                                    ggTACACAATGACTGAAATGAGGTTAACCCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCa                                                                                                                                                                                <  1:495155/250‑1 (MQ=255)                                                                    ggTACACAATGACTGAAATGAGGTTAAAGCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTGTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTTCGCCGAAGAGCAGGTAAATCATTCCCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGtttcc                                                                                                                                                                                 <  2:342256/249‑4 (MQ=255)                                                                    ggTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTAcc                                                                                                                                                                                 <  1:555721/249‑1 (MQ=255)                                                                    ggTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTAcc                                                                                                                                                                                 <  1:279800/249‑1 (MQ=255)                                                                          caATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGGGCACCAACAACCTGCGCCGAAGAGCAGGTAAAGCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGAATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTCAACGTTACCAATTGttt                                                                                                                                                                         >  2:226000/1‑251 (MQ=255)                                                                            aTGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTa                                                                                                                                                                        >  1:594094/1‑250 (MQ=255)                                                                              gACTGAAAGGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTTTTTaag                                                                                                                                                                      <  2:596751/250‑1 (MQ=255)                                                                                    aaTGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGTAGAAAAAATCTGGCATGCATATCCCTCTTTTGGCCGGTCGCGATGACTTACCTTTGTAAACGTTACCAATTTTTTAAGAAGtata                                                                                                                                                               <  2:418753/251‑1 (MQ=255)                                                                                        aGGTTAAACCGCCTGGCTGCGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGCAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGAATTTCCTGTGTAAACGTTAACAATTGTTTACGAAGTATATAcc                                                                                                                                                           >  2:59903/1‑250 (MQ=255)                                                                                             aaaCCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg                                                                                                                                                      <  1:35645/251‑1 (MQ=255)                                                                                               aCCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACg                                                                                                                                                      >  1:463880/1‑249 (MQ=255)                                                                                                 cGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGt                                                                                                                                                  <  2:191775/251‑1 (MQ=255)                                                                                                  gCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAGACTGGAGGATGTAGCGAAACTGCACAAATCCGGTGCGGAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCACAGGACGCTGTTAATAAAGGGGAAAAATTCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAACCGTTACCAATTGTTTAAGAAGAAAATACGCTACGAgga                                                                                                                                                  >  2:227326/1‑249 (MQ=255)                                                                                                   ccTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTAc                                                                                                                                                <  1:83412/251‑1 (MQ=255)                                                                                                        cTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGAt                                                                                                                                           >  1:379499/1‑251 (MQ=255)                                                                                                              aTGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTc                                                                                                                                     <  1:65616/251‑1 (MQ=255)                                                                                                                  aTGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑g                                                                                                                                <  2:379499/251‑1 (MQ=255)                                                                                                                  aTGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑g                                                                                                                                >  1:120031/1‑251 (MQ=255)                                                                                                                  aTGCTATCTTTAAGACTTTGGCCAAAATAGCGGATGTAGCAAAAATGCACAAATCCGGTGAGAAAAGTGAACCAAAAAACTGCGCCGAAGAGCAGGTCCATAATTACAGATACCCAAAGGAGGCTATTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGCCGCGATGACTTTACTGTGTAAAAGTTAACAATTGTTTAAGAAGTATATACCCCACGCGGTACTTGATCACTTCTGC‑g                                                                                                                                >  2:604180/1‑251 (MQ=255)                                                                                                                   tGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑Gt                                                                                                                               >  1:516892/1‑251 (MQ=255)                                                                                                                       aTTTTTAAGACGTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCGGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTGTCCTGTGTAAACGTTACCAATTGTGTAAGAAGTATATACGCGACGAGGTACTTTATAACGTCTGC‑GTAg                                                                                                                             <  2:365742/249‑1 (MQ=255)                                                                                                                         ttttAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCAt                                                                                                                          <  1:341578/250‑1 (MQ=255)                                                                                                                           ttAAGACGTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACGTGATAACTTCTGC‑GTAGCATAc                                                                                                                        <  2:79965/250‑1 (MQ=255)                                                                                                                            tAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACa                                                                                                                       <  1:110028/250‑1 (MQ=255)                                                                                                                             aaGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACGTCTGC‑GTAGCATACAt                                                                                                                      <  2:217588/250‑1 (MQ=255)                                                                                                                                      gCCAAACTGGCGGATGTAGAGAAACTGCACAAATCCGGTGGGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCACAACGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAAAGGTACCAATTGTTTAAGAAGAATATACGCTACGAGGTACTGGCAAACTTATGC‑GTAAACTACATG‑AGGTTTTGt                                                                                                           >  2:464481/1‑251 (MQ=255)                                                                                                                                            cTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGAATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATaaa                                                                                                      >  2:47165/1‑250 (MQ=255)                                                                                                                                            cTGGCGGATGTAGCGAAACCGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTACTAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGATACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATaaa                                                                                                      >  2:455854/1‑250 (MQ=255)                                                                                                                                              ggCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATaaaaa                                                                                                    <  1:520448/250‑1 (MQ=255)                                                                                                                                              ggCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATaaaaa                                                                                                    <  1:324198/250‑1 (MQ=255)                                                                                                                                                 ggATGTAGCGCAACTGCCCAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACg                                                                                                                                                                                      >  2:424209/1‑167 (MQ=255)                                                                                                                                                 ggATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACg                                                                                                                                                                                      <  1:424209/167‑1 (MQ=255)                                                                                                                                                   aTGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCGACGAGGTACGGGATAACGTCTGC‑GTAGCATACATG‑AGGTTTTGGAGAAAAATggcgg                                                                                              <  2:564457/251‑1 (MQ=255)                                                                                                                                                     gTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATggcggg                                                                                             <  1:445052/250‑1 (MQ=255)                                                                                                                                                         cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATggcgggcggt                                                                                         >  2:201852/1‑248 (MQ=255)                                                                                                                                                         cGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGata                                                                                        <  1:390620/251‑1 (MQ=255)                                                                                                                                                                gCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAATAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCGCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTTCGAGGTACTTGATAACTTCGGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACg                                                                                  <  1:601230/250‑1 (MQ=255)                                                                                                                                                                gCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑ATGTTTTGTAGAAAAATGGCGGGCGATATCAACGc                                                                                 <  2:451862/251‑1 (MQ=255)                                                                                                                                                                    aaaTCCGGTGCGAAAAGTGAACCCACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGCTCCCCAACGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGAATAAAAATGGCGGGCGAGATCAACGCaacg                                                                             >  2:375194/1‑248 (MQ=255)                                                                                                                                                                     aaTCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCAGACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAgtgt                                                                            <  1:281148/251‑1 (MQ=255)                                                                                                                                                                             ccGAAAAGTGAACCAACAACCTGCTCCGAAGAGCAGGTAAATCATTACCGATCCCCAAACGACGCTGTTAATAAAGGAGAAAAAAACTTGCATGCATATCCCTCTTATTGCCTGTCTCGATGACTTTCCGGTGTAAACGTGTCCAATGGTTTAATAAGTATTTACGCTACGATGTTCTCGATAACTTCTGCTGT‑GCTTAC‑TGTACGTTTTGTATAAAAATGGCGTGCGAGATCTAATCAGTGTCTGAAATc                                                                    <  2:277259/250‑1 (MQ=255)                                                                                                                                                                                      gAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCATTGTCAGAAATCCGAAACAGt                                                           <  2:552189/251‑1 (MQ=255)                                                                                                                                                                                              aacCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAA‑TCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGCTATCAACGCAGTGTAAGAAATCCGAAACAGTCTCGCCTgg                                                  >  2:431236/1‑251 (MQ=255)                                                                                                                                                                                                       cGAAGAGCAGGTAACTCATTACAGCTCCCCAACGGACGCTGTTAATAAAGGAGAAACAATATGGCATGCATATCCCTATTCTTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGACGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATaaac                                          >  2:298106/1‑249 (MQ=255)                                                                                                                                                                                                            agCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCtt                                     <  1:332006/251‑1 (MQ=255)                                                                                                                                                                                                               aGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTc                                  <  1:346000/251‑1 (MQ=255)                                                                                                                                                                                                                                               gTTAATAAAGGAGAAAAACTCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAAAGCAGGGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAAACGTCTTGGCGGCGGTTGCGCAGGCGCTGcgtcggcataatc  >  2:110290/1‑243 (MQ=255)                                                                                                                                                                                                                                               gTTAATAAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATatc   <  2:177502/250‑1 (MQ=255)                                                                                                                                                                                                                                               gTTAATAAAGGAGAAAAAATCTGGCATGCAGATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATatca  <  1:619826/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       GTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCAAGACTGGTACACAATGACTGAAATGAGGTTAAACCGCCTGGCTGTGGATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAACTGCACAAATCCGGTGCGAAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAAAGGACGCTGTTAATGAAGGAGAAAAAATCTGGCATGCATATCCCTCTTATTGCCGGTCGCGATGACTTTCCTGTGTAAACGTTACCAATTGTTTAAGAAGTATATACGCTACGAGGTACTTGATAACTTCTGC‑GTAGCATACATG‑AGGTTTTGTATAAAAATGGCGGGCGATATCAACGCAGTGTCAGAAATCCGAAACAGTCTCGCCTGGCGATAACCGTCTTGTCGGCGGTTGCGCTGACGTTGCGTCGTGATATCA  >  NC_000913/7695‑8183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑