Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,031	T→A	D30E (GAT→GAA)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,031	0	T	A	100.0%	144.2 / NA	46	D30E (GAT→GAA)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (30/16);  total (30/16)

AACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTA‑TTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTT  >  NC_000913/4640788‑4641277                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        aaCCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCgg                                                                                                                                                                                                                                                   >  1:16731/1‑250 (MQ=255)          tACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGAt                                                                                                                                                                                                                                          >  1:50148/1‑250 (MQ=255)                                 ttttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGagccagcc                                                                                                                                                                                                                  >  1:349534/1‑251 (MQ=255)                                  tttttAAGCAAAGTAAAGGGATTTTTCTGCGACTTACTTTAAGGATTTGTAAATTCGCGCCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑GTTGGTCAAGAAGAGTGGCAATATGCTTATAACGATTATTCTGTTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGATAACGATGGGTTTTAGCGAACTGCGGATTGTCGAGTGGCAGGCACACCTGGaaccagc                                                                                                                                                                                                                   <  2:563152/249‑1 (MQ=255)                                      tAAGCTAAGTAAAGGGCGTTTTCTGCGCCTTACGTTAAGGATTTGTAAATTCGAACCGCGTAATAAGTTGACAGTGACCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAAGATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAccc                                                                                                                                                                                                              <  2:143946/250‑1 (MQ=255)                                        aGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAACTATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGAGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCt                                                                                                                                                                                                           >  2:3815/1‑251 (MQ=255)                                          cTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTgg                                                                                                                                                                                                         >  2:141697/1‑251 (MQ=255)                                            aaGTAAAGGGATTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCATAGCAGAAACTATTGGGGCAGCGGCGCGGGCAATGAACACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCAGGAGCCAGCCACCCGCTgggg                                                                                                                                                                                                       >  1:110734/1‑250 (MQ=255)                                                 aaGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGcac                                                                                                                                                                                                  >  1:403144/1‑251 (MQ=255)                                                    ggCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATg                                                                                                                                                                                               <  1:135122/251‑1 (MQ=255)                                                    ggCTTTTTATGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATg                                                                                                                                                                                               <  2:213796/251‑1 (MQ=255)                                                         tttCGGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGGTCGCGGTTA‑TTGGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACTCCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATc                                                                                                                                                                                           <  2:530579/250‑1 (MQ=255)                                                          ttCTGCGACTTACGTTAAGGATTTGTAAGTTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTg                                                                                                                                                                                         >  2:162424/1‑251 (MQ=255)                                                           actggtaCTTACGTTAAGTATTTGTAAATTCGCACCGTGTAATAAGTTGACATTGATCCCCCTTTTCGCGGTTA‑TTTGTTCAAGAAGAGTGGTAATATGGTTAGAACGATTATTCGGGGCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAAGGAAAACGATGGGGTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGTAAGCCACCCGCTGGGTCGcacaatgaactg                                                                                                                                                                                         <  2:262232/244‑9 (MQ=255)                                                               cGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGCGCCAGCCACCCGCTGGCTCGCACATGGATCTGGTgct                                                                                                                                                                                    >  2:148228/1‑249 (MQ=255)                                                                        ttAAGGATTTGTAAATTAGCACCGCGTAATACGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGATGGGTCGCACATGGATCTGGTGATATTATTGat                                                                                                                                                                           >  1:458450/1‑251 (MQ=255)                                                                                gtGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGTTTCGCGGTTA‑TTTTGTCAAGAAGAGTGGAAATATGCGTATAACGATTATTCTGGGCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACACGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTa                                                                                                                                                                    <  2:148156/249‑1 (MQ=255)                                                                                  gTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTaa                                                                                                                                                                   >  1:537279/1‑249 (MQ=255)                                                                                     aaTTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGttt                                                                                                                                                              >  2:130806/1‑251 (MQ=255)                                                                                              cggttaataaGTGGACAGTGATTACCCGGTGCGCGGTTAGTTTG‑TAAAGAAGAGTGTCAATATGGGTATAACGATTATTCTGGTGGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCTGGCAATGAAAACGATGGGTGTTAGCGAACTGCGGAGTGTCGATAGGCAGGCACACCTTGAGCCAGTCTCCCGCTGGGTCGCACATGGAGCTGGTGATATTATTGATAATATTAAAGTTTG‑TCCGACac                                                                                                                                                    <  2:389553/247‑2 (MQ=255)                                                                                                    ataaGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTg                                                                                                                                              >  1:148497/1‑251 (MQ=255)                                                                                                                    cACCCTGTTCGCGGTTA‑TTTGGTCAAAAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATg                                                                                                                               <  2:16731/250‑1 (MQ=255)                                                                                                                         tgttcaccgTTA‑ATTGGTCAAGAAGAGAGGCAATATGCGTAGAACGATGATTCTGGTCGCACCCGCCAGAGCAGGAACTATTGGGGCAGCGGCGCGGGCACTGAACACGCTGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCAGGCCACCCGCGGGCGCGAACCTGGATCTGGGGATATTATTGAGAAACTTCCAGTTTT‑CCCGCCACTGTCGGCATCGTTACAAGATGTCgctt                                                                                                                         >  2:358617/9‑248 (MQ=255)                                                                                                                           ttCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTc                                                                                                                       >  1:253462/1‑251 (MQ=255)                                                                                                                             cgcgGGTA‑TTGGGTCAAGATGAGTGGCAATTGGCGTAAAGCGATTATTCTGGTCGCACCCGGCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGTAAGTCAGGCACACCTTGAGCGAGCCGCCCGCGTTGTCGCATATGGATCTGGTGATATTATGGAGAGTAGTAA‑GTTTGGCCCGACACTGACTGAATCGTTACGCGAGGGCGATTTCa                                                                                                                      <  2:541866/250‑1 (MQ=255)                                                                                                                                ggTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGt                                                                                                                  >  1:438484/1‑251 (MQ=255)                                                                                                                                             aagaagAGTGTCAATATGCGTATAACGATGATTCTGGTCTCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTgc                                                                                                      <  2:528951/251‑1 (MQ=255)                                                                                                                                                             tGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGaa                                                                                       >  2:174797/1‑250 (MQ=255)                                                                                                                                                                      cGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATtac                                                                             >  1:613308/1‑251 (MQ=255)                                                                                                                                                                      cGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATtac                                                                             >  1:175839/1‑251 (MQ=255)                                                                                                                                                                       gATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATtact                                                                            >  1:403666/1‑251 (MQ=255)                                                                                                                                                                            ttCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTacgct                                                                       >  1:335605/1‑250 (MQ=255)                                                                                                                                                                             tctggcggCACCCGACAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACTTTGGCTGAATCGTTACTCGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTacgct                                                                       <  1:408441/243‑2 (MQ=255)                                                                                                                                                                             tCTGGTCGCACCCGCCAGAGCCGACAATATTGGAGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGTTAGACAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTA‑CCCGACCCTGGCTGAATCGTTACACGCTGTCGCTTTCACTGTCGCCACCACAGCGCGCAGTCGGGCGCACTATCATtgctaagct                                                                       >  1:105790/1‑246 (MQ=255)                                                                                                                                                                                   cGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTacgctacgcc                                                                  >  1:61588/1‑249 (MQ=255)                                                                                                                                                                                   cGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTacgctacgcc                                                                  >  2:602121/1‑249 (MQ=255)                                                                                                                                                                                      ccccGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATTAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGtag                                                             <  1:552193/250‑3 (MQ=255)                                                                                                                                                                                      aCCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGACAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGt                                                               >  2:542980/1‑249 (MQ=255)                                                                                                                                                                                                gCAGAAAATATTGGGTCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCCCATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGtacc                                                   <  1:204285/251‑4 (MQ=255)                                                                                                                                                                                                         atTGGGGCCGCGGCACGACCAATGAAAACGATGGCTTTTAGCGAACTGCGGATTGTCGATAGTCCGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGCCACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAACTATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGtt                                             >  2:297865/1‑248 (MQ=255)                                                                                                                                                                                                         atTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTaga                                          <  2:175839/251‑1 (MQ=255)                                                                                                                                                                                                                cAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCCCACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGaaaa                                    >  2:562557/1‑250 (MQ=255)                                                                                                                                                                                                                      cgcgGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCa                             <  2:61588/251‑1 (MQ=255)                                                                                                                                                                                                                                 aaaaCGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGcc                   >  1:551329/1‑250 (MQ=255)                                                                                                                                                                                                                                         gggttttAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCgcgc          >  1:209162/5‑251 (MQ=255)                                                                                                                                                                                                                                                 gCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGttt  >  2:129547/1‑251 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                        AACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTA‑TTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCCATGCCGCGCTGGTGTTT  >  NC_000913/4640788‑4641277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑