Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,641,022	G→T	G27G (GGG→GGT)	yjtD →	putative tRNA/rRNA methyltransferase YjtD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,641,022	0	G	T	95.8%	137.8 / NA	48	G27G (GGG→GGT)	yjtD	putative tRNA/rRNA methyltransferase YjtD
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (30/16);  total (30/18)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTA‑TTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCC  >  NC_000913/4640778‑4641260                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ttAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGCAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGc                                                                                                                                                                                                                                           >  1:530579/1‑251 (MQ=255)  tAACAATTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGc                                                                                                                                                                                                                                           <  1:303010/250‑1 (MQ=255)      acttCAACCGTTAGTACCATCAGGAAATAGTTTCGCCTTTTTTACGCTAAGTACAGGGATTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACAGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCAGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGAGGGGTTTTAGCGaact                                                                                                                                                                                                                                      >  2:437795/4‑248 (MQ=255)        gtCAACCGTTAGTACATTCAGTAAAGAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGctaactg                                                                                                                                                                                                                                     <  2:28716/249‑7 (MQ=255)           aaCCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCgg                                                                                                                                                                                                                                  >  1:16731/1‑250 (MQ=255)                    tACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGAt                                                                                                                                                                                                                         >  1:50148/1‑250 (MQ=255)                                           ttttttAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGagccagcc                                                                                                                                                                                                 >  1:349534/1‑251 (MQ=255)                                            tttttAAGCAAAGTAAAGGGATTTTTCTGCGACTTACTTTAAGGATTTGTAAATTCGCGCCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑GTTGGTCAAGAAGAGTGGCAATATGCTTATAACGATTATTCTGTTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGATAACGATGGGTTTTAGCGAACTGCGGATTGTCGAGTGGCAGGCACACCTGGaaccagc                                                                                                                                                                                                  <  2:563152/249‑1 (MQ=255)                                                tAAGCTAAGTAAAGGGCGTTTTCTGCGCCTTACGTTAAGGATTTGTAAATTCGAACCGCGTAATAAGTTGACAGTGACCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAAGATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCAccc                                                                                                                                                                                             <  2:143946/250‑1 (MQ=255)                                                  aGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAACTATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGAGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCt                                                                                                                                                                                          >  2:3815/1‑251 (MQ=255)                                                    cTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTgg                                                                                                                                                                                        >  2:141697/1‑251 (MQ=255)                                                      aaGTAAAGGGATTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCATAGCAGAAACTATTGGGGCAGCGGCGCGGGCAATGAACACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCAGGAGCCAGCCACCCGCTgggg                                                                                                                                                                                      >  1:110734/1‑250 (MQ=255)                                                           aaGGGCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGcac                                                                                                                                                                                 >  1:403144/1‑251 (MQ=255)                                                              ggCTTTTTCTGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATg                                                                                                                                                                              <  1:135122/251‑1 (MQ=255)                                                              ggCTTTTTATGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATg                                                                                                                                                                              <  2:213796/251‑1 (MQ=255)                                                                   tttCGGCGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGGTCGCGGTTA‑TTGGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACTCCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATc                                                                                                                                                                          <  2:530579/250‑1 (MQ=255)                                                                    ttCTGCGACTTACGTTAAGGATTTGTAAGTTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTg                                                                                                                                                                        >  2:162424/1‑251 (MQ=255)                                                                     actggtaCTTACGTTAAGTATTTGTAAATTCGCACCGTGTAATAAGTTGACATTGATCCCCCTTTTCGCGGTTA‑TTTGTTCAAGAAGAGTGGTAATATGGTTAGAACGATTATTCGGGGCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAAGGAAAACGATGGGGTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGTAAGCCACCCGCTGGGTCGcacaatgaactg                                                                                                                                                                        <  2:262232/244‑9 (MQ=255)                                                                         cGACTTACGTTAAGGATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGCGCCAGCCACCCGCTGGCTCGCACATGGATCTGGTgct                                                                                                                                                                   >  2:148228/1‑249 (MQ=255)                                                                                  ttAAGGATTTGTAAATTAGCACCGCGTAATACGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGATGGGTCGCACATGGATCTGGTGATATTATTGat                                                                                                                                                          >  1:458450/1‑251 (MQ=255)                                                                                          gtGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGTTTCGCGGTTA‑TTTTGTCAAGAAGAGTGGAAATATGCGTATAACGATTATTCTGGGCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACACGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTa                                                                                                                                                   <  2:148156/249‑1 (MQ=255)                                                                                            gTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTaa                                                                                                                                                  >  1:537279/1‑249 (MQ=255)                                                                                               aaTTCGCACCGCGTAATAAGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGttt                                                                                                                                             >  2:130806/1‑251 (MQ=255)                                                                                                        cggttaataaGTGGACAGTGATTACCCGGTGCGCGGTTAGTTTG‑TAAAGAAGAGTGTCAATATGGGTATAACGATTATTCTGGTGGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCTGGCAATGAAAACGATGGGTGTTAGCGAACTGCGGAGTGTCGATAGGCAGGCACACCTTGAGCCAGTCTCCCGCTGGGTCGCACATGGAGCTGGTGATATTATTGATAATATTAAAGTTTG‑TCCGACac                                                                                                                                   <  2:389553/247‑2 (MQ=255)                                                                                                              ataaGTTGACAGTGATCACCCTGTTCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTg                                                                                                                             >  1:148497/1‑251 (MQ=255)                                                                                                                              cACCCTGTTCGCGGTTA‑TTTGGTCAAAAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATg                                                                                                              <  2:16731/250‑1 (MQ=255)                                                                                                                                   tgttcaccgTTA‑ATTGGTCAAGAAGAGAGGCAATATGCGTAGAACGATGATTCTGGTCGCACCCGCCAGAGCAGGAACTATTGGGGCAGCGGCGCGGGCACTGAACACGCTGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCAGGCCACCCGCGGGCGCGAACCTGGATCTGGGGATATTATTGAGAAACTTCCAGTTTT‑CCCGCCACTGTCGGCATCGTTACAAGATGTCgctt                                                                                                        >  2:358617/9‑248 (MQ=255)                                                                                                                                     ttCGCGGTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTc                                                                                                      >  1:253462/1‑251 (MQ=255)                                                                                                                                       cgcgGGTA‑TTGGGTCAAGATGAGTGGCAATTGGCGTAAAGCGATTATTCTGGTCGCACCCGGCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGTAAGTCAGGCACACCTTGAGCGAGCCGCCCGCGTTGTCGCATATGGATCTGGTGATATTATGGAGAGTAGTAA‑GTTTGGCCCGACACTGACTGAATCGTTACGCGAGGGCGATTTCa                                                                                                     <  2:541866/250‑1 (MQ=255)                                                                                                                                          ggTTA‑TTTGGTCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGt                                                                                                 >  1:438484/1‑251 (MQ=255)                                                                                                                                                       aagaagAGTGTCAATATGCGTATAACGATGATTCTGGTCTCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTgc                                                                                     <  2:528951/251‑1 (MQ=255)                                                                                                                                                                       tGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGaa                                                                      >  2:174797/1‑250 (MQ=255)                                                                                                                                                                                cGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATtac                                                            >  1:613308/1‑251 (MQ=255)                                                                                                                                                                                cGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATtac                                                            >  1:175839/1‑251 (MQ=255)                                                                                                                                                                                 gATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATtact                                                           >  1:403666/1‑251 (MQ=255)                                                                                                                                                                                      ttCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTacgct                                                      >  1:335605/1‑250 (MQ=255)                                                                                                                                                                                       tctggcggCACCCGACAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACTTTGGCTGAATCGTTACTCGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTacgct                                                      <  1:408441/243‑2 (MQ=255)                                                                                                                                                                                       tCTGGTCGCACCCGCCAGAGCCGACAATATTGGAGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGTTAGACAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTA‑CCCGACCCTGGCTGAATCGTTACACGCTGTCGCTTTCACTGTCGCCACCACAGCGCGCAGTCGGGCGCACTATCATtgctaagct                                                      >  1:105790/1‑246 (MQ=255)                                                                                                                                                                                             cGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTacgctacgcc                                                 >  1:61588/1‑249 (MQ=255)                                                                                                                                                                                             cGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTacgctacgcc                                                 >  2:602121/1‑249 (MQ=255)                                                                                                                                                                                                ccccGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATTAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGtag                                            <  1:552193/250‑3 (MQ=255)                                                                                                                                                                                                aCCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGACAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGt                                              >  2:542980/1‑249 (MQ=255)                                                                                                                                                                                                          gCAGAAAATATTGGGTCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCCCATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGtacc                                  <  1:204285/251‑4 (MQ=255)                                                                                                                                                                                                                   atTGGGGCCGCGGCACGACCAATGAAAACGATGGCTTTTAGCGAACTGCGGATTGTCGATAGTCCGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGCCACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAACTATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGtt                            >  2:297865/1‑248 (MQ=255)                                                                                                                                                                                                                   atTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTaga                         <  2:175839/251‑1 (MQ=255)                                                                                                                                                                                                                          cAGCGGCGCGGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCCCACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGaaaa                   >  2:562557/1‑250 (MQ=255)                                                                                                                                                                                                                                cgcgGGCAATGAAAACGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCa            <  2:61588/251‑1 (MQ=255)                                                                                                                                                                                                                                           aaaaCGATGGGTTTTAGCGAACTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACACTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCTACGCCAGTAGAACTGGTACCGCTGTTAGAGGAAAAATCTTCATGGATGAGcc  >  1:551329/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TTAACAACTCAACCGTTAGTACAGTCAGGAAATAGTTTAGCCTTTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAATTTGTAAATTCGCACCGCGTAATAAGTTGACAGTGATCACCCGGTTCGCGGTTA‑TTTGATCAAGAAGAGTGGCAATATGCGTATAACGATTATTCTGGTCGCACCCGCCAGAGCAGAAAATATTGGGGCAGCGGCGCGGGCAATGAAAACGATGGGGTTTAGCGATCTGCGGATTGTCGATAGTCAGGCACACCTGGAGCCAGCCACCCGCTGGGTCGCACATGGATCTGGTGATATTATTGATAATATTAAAGTTTT‑CCCGACATTGGCTGAATCGTTACACGATGTCGATTTCACTGTCGCCACCACTGCGCGCAGTCGGGCGAAATATCATTACTACGCCACGCCAGTTGAACTGGTGCCGCTGTTAGAGGAAAAATCTTCATGGATGAGCC  >  NC_000913/4640778‑4641260

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑