Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,719	C→T	P196P (CCG→CCA)	arcA ←	DNA‑binding transcriptional dual regulator ArcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,719	0	C	T	100.0%	194.3 / NA	64	P196P (CCG→CCA)	arcA	DNA‑binding transcriptional dual regulator ArcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/19);  total (45/19)

CCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGA  >  NC_000913/4639484‑4639952                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           ccAGTAAAGACGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTAGTGTGGTTTCCGCTCACgg                                                                                                                                                                                                                             >  2:257849/1‑251 (MQ=255)     tAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCCCGTCTACAGTAAGGTCGTGTGGTTTCAACTCACAGCCgg                                                                                                                                                                                                                         >  2:354842/1‑251 (MQ=255)      aaaGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGt                                                                                                                                                                                                                        >  1:302947/1‑251 (MQ=255)       aagaagTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTc                                                                                                                                                                                                                       <  1:591048/251‑1 (MQ=255)                   tggACGATCACTTACGTATCTGGGAATAAGGTTGAAAAATACAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGCTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTAACGTACGGTAGAGTGGTTTCCGCTCACGGCCGGACAtttttattagc                                                                                                                                                                                                           >  2:360348/2‑244 (MQ=255)                      acgatcaattACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTcagcagg                                                                                                                                                                                                        >  2:217217/9‑250 (MQ=255)                       cgatcaattACGTATCTGGAAATAAGGTTTAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGAGCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGtt                                                                                                                                                                                                       <  1:362985/244‑1 (MQ=255)                       cgatcaattACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGtt                                                                                                                                                                                                       >  1:367522/8‑251 (MQ=255)                            aattACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCTGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGc                                                                                                                                                                                                   >  1:282906/3‑250 (MQ=255)                             attACGTATCTGGAAGTAAGGTTGAAATATAAAAACGGCGCTCAAAAGCGCCGTTTTTTTTGACGGAGGTAAAGACGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCATCACGTCTACAGTACGGTAGTGTGGTTTCAGCTCACGGACGGTCAtttcattccgtcgttcagcac                                                                                                                                                                                                 >  2:424203/2‑233 (MQ=255)                                           aaTAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTg                                                                                                                                                                                   <  1:471639/251‑1 (MQ=255)                                           aaTAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGGTTTTTTTGACGGGGGTAAAACCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGGCTACAGTACGGGCGTGTGGTTTCAGCTCACTGCCGGTCATTTTCTTGCGCAGTTCCGCACAGGCCTGCATTTTg                                                                                                                                                                                   >  2:160288/1‑251 (MQ=255)                                                     aaaaaTAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGAAGGAGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCCGCTCACGGCCGGGCATTTTCTTCAGCAGTTCAGCACGAGACTGCATTTTGCCTGGGtttt                                                                                                                                                                         >  2:558927/1‑251 (MQ=255)                                                       aaaTAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGAGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCCACAGTACGGTCGTGTGGGTTCAGCTCACGGCCGGGCACTTTCTTCCACCGTTCCGCGAGCGACTGAATTTTGCCTGGGttttt                                                                                                                                                                        >  2:40207/1‑249 (MQ=255)                                                       aaaTAAAAACGGCGCTAAAAAACGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGAGGCGATGATTTCCGGCGAATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATAGTCCCGTCTACAGTACAGTCGGGTGGTTTCAGCTCACGGCCGGTCATTTTCTGCAGCAGTTAAGCACGAGACTGCATTTTGCCTGGGTTTTc                                                                                                                                                                        >  1:355895/1‑250 (MQ=255)                                                              aaCGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAgg                                                                                                                                                                >  2:452962/1‑250 (MQ=255)                                                                cGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCATCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACCGAAGTg                                                                                                                                                               >  2:479992/1‑250 (MQ=255)                                                                      tAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCCCAGAAGTGAAGCATg                                                                                                                                                        >  2:159006/1‑251 (MQ=255)                                                                        aaaaGCGCCGTCTTTCTTGACGATGGTAAAGCCGATTAAACTTCCAGATCACCACAGAAGCGATACCATTCAACGTGGATGGTGGCGATGATTTTCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATCCGGCGGATCGTCACGGCTACAGTAAGGTTGAGTGGTTTAAGCGAACGGCCGGGCATTTTCTTCCGCCGTTCAGCCCGAGACAGAATTTTGCGTGGGATTTCACAGAAGGGAAGCAGGgc                                                                                                                                                      >  2:520479/1‑251 (MQ=255)                                                                         aaaGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGgcc                                                                                                                                                     >  2:188763/1‑250 (MQ=255)                                                                                         tGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTAAGGTCGTGTGGTTTCAGCTCACGGCCGGGCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCCCTGcgcg                                                                                                                                     >  2:182313/1‑251 (MQ=255)                                                                                                   aaaGCCGATTAATCTTCCAGATCACCACCGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTa                                                                                                                           <  2:332873/251‑1 (MQ=255)                                                                                                    aaGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACCGAAGTGAAGCCTGGCGCGGAACTCGCTGCGCGGCCGCTTGTa                                                                                                                           >  2:522579/1‑250 (MQ=255)                                                                                                     aGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACt                                                                                                                         <  1:479992/251‑1 (MQ=255)                                                                                                            tAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGcc                                                                                                                  >  1:362214/1‑251 (MQ=255)                                                                                                             aaTCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTAAGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGAATGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAAATCGCTGCGCGGCAGCTTGTACTGCTCGcc                                                                                                                  >  2:12451/1‑250 (MQ=255)                                                                                                                   ccAGAGCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGATTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAgg                                                                                                            <  1:341032/250‑1 (MQ=255)                                                                                                                   acAGAGCACCCCAGAAGCGATAACCTTCACCGTGGCTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGAAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTAATGCTCGCCATCAggg                                                                                                           >  2:418165/2‑251 (MQ=255)                                                                                                                    cAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGGTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGc                                                                                                          >  1:369327/1‑251 (MQ=255)                                                                                                                       aTCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCAGGCGTATCCGGCGTAGCTTCGAAATGTTTACGAATACGGCGGATCGTCCCGTCTACAGTACGGTTGTGTGGATTCAGCTCCAGGCCGGTCATTTTCTTCAGCTGTTAAGAACGAGAATGAATTTTGCCTGGGTTTTAACAGGAGTGAAGCATGGCGCGGCACTCGCTGGCCGGCAGGTTGTACTGCTCGCCATCAGGGCCg                                                                                                        >  2:426478/1‑250 (MQ=255)                                                                                                                             acAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCaacg                                                                                                 <  2:287072/250‑1 (MQ=255)                                                                                                                                   gCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGc                                                                                           >  2:214736/1‑251 (MQ=255)                                                                                                                                     gATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGGTTTCACAGAAGTGAAGCATGGTGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTg                                                                                         >  1:205060/1‑251 (MQ=255)                                                                                                                                        aaCCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCCTTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTTCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑g                                                                                     >  1:87287/1‑251 (MQ=255)                                                                                                                                                ccGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAg                                                                             <  1:419970/251‑1 (MQ=255)                                                                                                                                                ccGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAg                                                                             <  2:52521/251‑1 (MQ=255)                                                                                                                                                  gtggaTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGtt                                                                           >  1:498334/5‑251 (MQ=255)                                                                                                                                                  gtggaTGGTGGCGATGATTTCCGGCGGATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCGGATCAACGAACGGCTGTT‑GATGTCCAGtt                                                                           <  1:532058/247‑1 (MQ=255)                                                                                                                                                    ggaTGGTGGCGATGATTTCCGGCGTATCCGGCGTCGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGGACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCCCGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAAATCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GTTGTCCAGTTcc                                                                         >  2:55307/3‑251 (MQ=255)                                                                                                                                                          tggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCaccc                                                                    >  1:349400/1‑247 (MQ=255)                                                                                                                                                           ggCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCAt                                                                  >  1:302601/1‑251 (MQ=255)                                                                                                                                                              gatgatTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTg                                                                >  1:517530/1‑250 (MQ=255)                                                                                                                                                               atgatTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCCGTTCCCAACCATTGa                                                               >  1:411082/1‑250 (MQ=255)                                                                                                                                                                      ccggcgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGCAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCCGTTCCCAACCATTGAACTTGt                                                         >  2:240876/1‑249 (MQ=255)                                                                                                                                                                        ggcgtatccggcgtaGATTCGAAATGTTTACGAATACGGCGGATTGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCCTCAGGGCCGATCAACGACCGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCt                                                     >  1:346894/1‑251 (MQ=255)                                                                                                                                                                          cgtatccggcgtaGATTCGAAAGTTTTACGAATGCGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCTCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTTTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCttt                                                   <  1:582499/251‑1 (MQ=255)                                                                                                                                                                          cgtatacggcgtaGATTCGAAATGTTTACGAATACGGCGGCTCGTCACGTCTACAGTCCGGTCGAGTGGGTTGGGCCCACGGCCGGTCATTTTCTTCAACAGTTCAGCAAGAGACTGCATTTTGCCTGGGTTTTCCCCGAAGCGAAGCATTGCGCGGGCCTCGCTGGGCGGCACATTGTAACGCTTGCAATCAGGGCCGATCAACGAACGGCCGTT‑GCTGTCCAGTTCCCAACCATTGAACTTGTAGCttt                                                   >  2:278394/1‑251 (MQ=255)                                                                                                                                                                            tatccggcgtaGATTAGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTAAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGCATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAAATCGCTGCGCGGCAGCTTGTACTCCTCGCCATCAGGGCCGATCAAAGAACGGCTGTTTGTTG‑CCAGTTCCCCACCATTGAACCTGTAGCTTTc                                                  >  2:62681/1‑250 (MQ=255)                                                                                                                                                                                           cGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCCCGGCCGGTCATTTTCTTCAGCAGTTCCGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCTGCAGCTTGTCCTGCTCGCCATCCGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGCACTTTTAGCTTTCAACGCTACGACGttct                                  >  2:515106/1‑251 (MQ=255)                                                                                                                                                                                            gAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGttctt                                 <  2:175502/251‑1 (MQ=255)                                                                                                                                                                                              aaTGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCg                               <  1:240876/251‑1 (MQ=255)                                                                                                                                                                                                   ttACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGa                           <  1:233316/250‑1 (MQ=255)                                                                                                                                                                                                         aTACGGCGGATCGTCACGTCTCCAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCCTTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAcc                    >  2:407376/1‑251 (MQ=255)                                                                                                                                                                                                         aTACGGCGGATCGTAACGTCTACAGTACGGTCGCGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTTCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACAGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAcc                    >  1:458478/1‑251 (MQ=255)                                                                                                                                                                                                           aCGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTAccc                   <  2:265431/250‑1 (MQ=255)                                                                                                                                                                                                            cggcggATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCAGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGACGTGAAGCATGGCGCGGAACTCGATGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGCCAGTAccc                   >  1:292572/1‑249 (MQ=255)                                                                                                                                                                                                              gcggATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGTACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGTCAGTACCCAGAt               <  2:45093/251‑1 (MQ=255)                                                                                                                                                                                                                ggATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTc             >  1:252580/1‑251 (MQ=255)                                                                                                                                                                                                                     gTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTTCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGt        >  1:437348/1‑251 (MQ=255)                                                                                                                                                                                                                     gTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGt        >  1:223454/1‑251 (MQ=255)                                                                                                                                                                                                                       cACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCGTCGCTGACAGTACCCAGATTCATGGTAc      <  2:539101/251‑1 (MQ=255)                                                                                                                                                                                                                           tCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACggg   >  2:35344/1‑250 (MQ=255)                                                                                                                                                                                                                              aCAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCAGTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGa  <  2:282906/248‑1 (MQ=255)                                                                                                                                                                                                                              aCAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGAGACTGAATTTTGCCTGGGGTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGa  <  1:424203/248‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           CCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGCAGTTCAGCACGGGACTGAATTTTGCCTGGGTTTTCACAGAAGTGAAGCATGGCGCGGAACTCGCTGCGCGGCAGCTTGTACTGCTCGCCATCAGGGCCGATCAACGAACGGCTGTT‑GATGTCCAGTTCCCAACCATTGAACTTGTAGCTTTCAACGCTACGACGTTCTTCGCTGACAGTACCCAGATTCATGGTACGGGA  >  NC_000913/4639484‑4639952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑