Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,639,510	G→C	E445Q (GAG→CAA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,639,510	0	G	C	100.0%	133.8 / NA	41	E445Q (GAG→CAA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (22/19);  total (22/19)

CGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGC  >  NC_000913/4639269‑4639752                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   cGGTATTTATTTGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTa                                                                                                                                                                                                                                           <  1:17349/251‑1 (MQ=255)     atttatttGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTg                                                                                                                                                                                                                                       <  2:459231/251‑1 (MQ=255)        tatttGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTGCCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGa                                                                                                                                                                                                                                     <  1:379892/250‑1 (MQ=255)            tGCAGGCGGTATTGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATa                                                                                                                                                                                                                                 <  1:564946/250‑1 (MQ=255)                   ggTATTCAACGGCTGGCGCAACAGCATGCTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGCCTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAAACAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGCCTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGa                                                                                                                                                                                                                          >  2:299397/1‑250 (MQ=255)                       ttGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAAt                                                                                                                                                                                                                     >  2:591048/1‑251 (MQ=255)                        tGAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAAt                                                                                                                                                                                                                     >  1:6857/1‑250 (MQ=255)                         gAAAGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaa                                                                                                                                                                                                                   <  2:283715/251‑1 (MQ=255)                          aaaGGCTGGCGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATaa                                                                                                                                                                                                                   >  1:435471/1‑250 (MQ=255)                            cggctggcgCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGCTGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCCTGCTGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCACTTACGTATCTGGAAATAAGGTTGAAAAATaaaa                                                                                                                                                                                                                 >  2:341032/9‑250 (MQ=255)                                tggcgCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGgc                                                                                                                                                                                                            >  1:265431/5‑251 (MQ=255)                                         aGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAg                                                                                                                                                                                                   <  1:44797/251‑1 (MQ=255)                                              gttgttTACCCTCGCGCTGTTGGTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTTCTGGTGGTGGCGCTGGCCGGCATTATGTTTGTGACTCGTAATAACGACTAGTATGCGTTTTCACTGCCGAAAAGGAAAGCCAGTAAAGAATTTACAAGGGACGATCAATTACGTATCTGGAAAGAAGGTTGAAAAATAAAAACGGCTCTAAAAAGCGCCg                                                                                                                                                                                              <  2:598626/251‑1 (MQ=255)                                                       ccTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTg                                                                                                                                                                                     >  1:175502/1‑251 (MQ=255)                                                            ggcTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCCGTGTGCTGGTGGTGGCGCTGGCCTGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGTCAGTAAAGAAGTTTCAATGGACGATCAATTACGTATCTGGAAATAAGGGGGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACggt                                                                                                                                                                                <  1:299397/250‑1 (MQ=255)                                                                      tcTGGATGGGGTTATTTGTGGACTGCTCAACTATGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGTCATGATGTTTGTGACTCGGAATATCGACTGGTAGGCGTGTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACTATCAATTACGTATCTTGAAATAAGGGTGAAAAATAAAAACTGCGCTAAAAAGCGCCGTTTGTTGTGTCTGTTGTAAAGcc                                                                                                                                                                       <  2:209217/249‑1 (MQ=255)                                                                       cgggATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAGGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTGGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGa                                                                                                                                                                     <  2:219120/249‑1 (MQ=255)                                                                       cTGGATGATCTGGTGTGGGGACTGCTCAACTCTGCGGATAGCGCGCTGTTGATAGGACCCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGAATGGTATGCGGTTTCACTGCCGAAAATGAAAGACAGTAAAGAAGTTACAATGGACGATCAATTACGTCTCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTCAAAAGCGCCGTTTTTTTTGACGGTGGTAAAgacg                                                                                                                                                                      >  2:615922/1‑247 (MQ=255)                                                                                          gACTGCTAAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGGTGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCAcc                                                                                                                                                   >  2:419970/1‑250 (MQ=255)                                                                                                 cAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGaa                                                                                                                                             <  2:458393/249‑1 (MQ=255)                                                                                                 cAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAg                                                                                                                                            <  1:123981/250‑1 (MQ=255)                                                                                                         ccGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTACTCTTCCAGATCACCACAGAAGCGATAACCt                                                                                                                                   >  1:518856/1‑251 (MQ=255)                                                                                                                  cgcTGTTGTTGGGTACCACTGACCTGGTGGTGGCGATCGCCGCAATGATGTATGAGACCCGAAATATCGTCTGGTATGCGTTTTCAATGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGCCGCTCACTTACGTATCTGGAAATCAGGTTGAAAACTAAAAACGGCGCTAACAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTACTCTTCCGGATCACACCAGAAGCGATacactccacagtgg                                                                                                                          >  2:567781/1‑238 (MQ=255)                                                                                                                               aaCCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGCAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGTTGGTGGCgatg                                                                                                              >  2:179229/1‑250 (MQ=255)                                                                                                                               aaCCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCgatg                                                                                                              >  1:539101/1‑250 (MQ=255)                                                                                                                                 ccAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGAtt                                                                                                           <  1:527758/251‑1 (MQ=255)                                                                                                                                     ggtgCTGGTGGTGGGGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTtccg                                                                                                       <  2:426933/250‑1 (MQ=255)                                                                                                                                       tgCTGGTGGGGGCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGCCTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGGGGCGATGATTtccggc                                                                                                     >  2:532058/1‑251 (MQ=255)                                                                                                                                                 ggCGCTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTtccggcgtatccggc                                                                                            <  2:85577/250‑1 (MQ=255)                                                                                                                                                    gcTGGCCGGCATGATGTTTGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCCCAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTtccggcgtatccggcgt                                                                                          >  1:52521/1‑249 (MQ=255)                                                                                                                                                       ggatggCATGATGGTTGTGAGTCGTAATATCGACTGGTATGGGTGGTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAAGTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGTGCGAAAAAGCGCCGGTTTTTTTGTCGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGTGATAACCGTGAGCGTGGATGGTGGCGAAGGTGTCCGGCCTAGCCGGCGTAGAtt                                                                                     <  2:242157/247‑1 (MQ=255)                                                                                                                                                                    tttGTGACTCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACg                                                                        >  1:287072/1‑251 (MQ=255)                                                                                                                                                                         gactcGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAc                                                                   <  2:281787/247‑1 (MQ=255)                                                                                                                                                                          actcGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATAcg                                                                  <  2:266957/248‑1 (MQ=255)                                                                                                                                                                                                  ttttCACTGCCGAAAATGAAATCCAGTAAAGAAGTTACAATGGACGCTCAAGTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTATAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAACGATAAACTTCACCGTGGATGTTGGAGATGATTTCCGGCGTATCCGGCGTAGATTCGAACTGTTTACGAATACTGCGGATCGTCACGTGTACAGtgcg                                          >  2:620052/1‑248 (MQ=255)                                                                                                                                                                                                   tttCACTGCCGAACCTGAAAGCCAGTAAAGAAGTTGCCATGGACGATCAGTTACGTATCTGGACATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACgg                                         >  2:90315/1‑251 (MQ=255)                                                                                                                                                                                                                        ccAGTAAAGACGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTAGTGTGGTTTCCGCTCACgg                    >  2:257849/1‑251 (MQ=255)                                                                                                                                                                                                                            tAAAGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCCCGTCTACAGTAAGGTCGTGTGGTTTCAACTCACAGCCgg                >  2:354842/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaGAAGTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGt               >  1:302947/1‑251 (MQ=255)                                                                                                                                                                                                                              aagaagTTACAATGGACGATCAATTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGTGGTTTCAGCTCACGGCCGGTc              <  1:591048/251‑1 (MQ=255)                                                                                                                                                                                                                                          tggACGATCACTTACGTATCTGGGAATAAGGTTGAAAAATACAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCACAGAAGCGATAACCTTCACCGTGGCTGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTAACGTACGGTAGAGTGGTTTCCGCTCACGGCCGGACAtttttattagc  >  2:360348/2‑244 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   CGGTATTTATTTGCAAGCGGTATTGAAAGGTTGGTGCAACAGCATGTTGTTTACCCTCGCGCTGTTGTTGCTGGATGGTGTGATGTGGGGACTGCTCAACTCTGCCGATAGCGCGCTGTTGTTGGGAACCAGTGTGCTGGTGGTGGCGCTGGCCGGCATGATGTTTGTGACCCGTAATATCGACTGGTATGCGTTTTCACTGCCGAAAATGAAAGCCAGTAAAGAAGTTACAACGGACGATGAGTTACGTATCTGGAAATAAGGTTGAAAAATAAAAACGGCGCTAAAAAGCGCCGTTTTTTTTGACGGTGGTAAAGCCGATTAATCTTCCAGATCACCGCAGAAGCGATAACCTTCACCGTGAATGGTGGCGATGATTTCCGGCGTATCCGGCGTAGATTCGAAATGTTTACGAATACGGCGGATCGTCACGTCTACAGTACGGTCGTGCGGTTTCAGCTCACGGCCGGTCATTTTCTTCAGC  >  NC_000913/4639269‑4639752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑