Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,681	A→G	F93F (TTT→TTC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,681	0	A	G	100.0%	190.2 / NA	57	F93F (TTT→TTC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/24);  total (33/24)

CAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAA  >  NC_000913/7437‑7924                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg                                                                                                                                                                                                                                                    <  1:232223/251‑1 (MQ=255)  aaaaGTAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACgg                                                                                                                                                                                                                                                   <  1:620114/251‑1 (MQ=255)      aaaaaaCCCAGGGCTCGGCCAACGGCTTTCGCCTGAATGCCGCTGAAAATGATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCTCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGTTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAATACGGCTcc                                                                                                                                                                                                                                               <  2:288932/250‑1 (MQ=255)         aaaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAgg                                                                                                                                                                                                                                            >  1:483979/1‑251 (MQ=255)         aaaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCAAgg                                                                                                                                                                                                                                            >  2:260682/1‑251 (MQ=255)          aaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGt                                                                                                                                                                                                                                           >  2:548345/1‑251 (MQ=255)           aCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTc                                                                                                                                                                                                                                          >  2:86900/1‑251 (MQ=255)              cAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTcc                                                                                                                                                                                                                                         <  1:18816/249‑1 (MQ=255)                     ggcgcagcGGCGTTCGCCTGAATCCCGCTAAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCATCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAATCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcg                                                                                                                                                                                                                                  <  2:195630/242‑1 (MQ=255)                                   cgcctGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCAGCGCac                                                                                                                                                                                                                  >  2:279800/5‑250 (MQ=255)                                              cGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGAAATTGACAGCGCAACGCCGGCCAgg                                                                                                                                                                                                       >  1:463767/1‑251 (MQ=255)                                                cTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACACCGGCCAGGtt                                                                                                                                                                                                     >  1:87187/1‑251 (MQ=255)                                                  gAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                                                                                                                                     <  1:531352/249‑1 (MQ=255)                                                            ccgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGTTCCCGcgccccac                                                                                                                                                                                         >  2:350145/4‑244 (MQ=255)                                                                 ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCACGCGCGCCATATACCATGCCGGTCCGCCGCGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGCGGGAACATTCGGCAAACGAGGTCGCCATGCCGCTAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCAGCGGAACGCCGGCCCGGTTGACGGTACCCAcgcgac                                                                                                                                                                                    >  1:362641/1‑249 (MQ=255)                                                                  gCGATGAGCAAAACGACGGCGAACAGAACGCCAATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGGTGCCGCCACCCACccgcgc                                                                                                                                                                                   >  1:499886/1‑251 (MQ=255)                                                                      tGAGCAAAACGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGACGGCCAGGGTTCCGCCACCCACGGGcgacgc                                                                                                                                                                                >  1:418753/1‑250 (MQ=255)                                                                                gACGGCGAACATACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTac                                                                                                                                                                    <  1:249570/251‑1 (MQ=255)                                                                                 aCGGCGAACAGCACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACACCGGCCAGGGTGCCGCTACCCCCGCGcgccgccacacagggacc                                                                                                                                                                    >  2:450849/1‑239 (MQ=255)                                                                                  cGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTaca‑c                                                                                                                                                                 <  1:440603/251‑1 (MQ=255)                                                                                    gCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑Caa                                                                                                                                                               <  1:607514/251‑1 (MQ=255)                                                                                      gAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAtt                                                                                                                                                                                                                            <  1:172086/190‑2 (MQ=255)                                                                                                     ccAGCGCATCCCCAGCCCGCGCGCCATATCCCTGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTa                                                                                                                                              <  2:159119/251‑1 (MQ=255)                                                                                                      cAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGACATGAgggta                                                                                                                                              >  1:79965/1‑247 (MQ=255)                                                                                                         cgcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAAcc                                                                                                                                          <  1:623283/251‑1 (MQ=255)                                                                                                          gcATCCCCAGCCCGCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCg                                                                                                                                         <  1:383276/251‑1 (MQ=255)                                                                                                          gcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGTTGCCGCTACCCCCGCGCGCCTCA‑AGACTGGGACA‑CACAGACTGAAATGAGGTTAAAccc                                                                                                                                         >  1:114212/1‑250 (MQ=255)                                                                                                              ccccAGCCCCCGCGCCATAGCCCATGCCGGTCCGCCACGAAACTGCCCATTTGCACCCCTTTCTTTATAAAGCTGGGCGAGGGAACATTCGGCAAACGAGGTCGCCAGGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTg                                                                                                                                     <  2:463767/251‑1 (MQ=255)                                                                                                                         cccccaaaacccATGCCGGCCCGCCCCGAAACGGCCCATTGGCATCACTTTCTTGATAAAGCTGTGCGAGGGAACATTCGGAAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGa                                                                                                                         <  2:548305/241‑1 (MQ=255)                                                                                                                               tataCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCCGCGCCACGACGGCCCGGTTGCCGCTCCCCACGCCCGCCCCC‑CGCGTGGGACA‑CAATGACTTGAATGAGGGTAAAcccgccgggtgggggggcatgatt                                                                                                                    >  2:288852/1‑229 (MQ=255)                                                                                                                                 aaCCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACGGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCGGTG‑GAGGAATGCTAtt                                                                                                                 <  2:116635/250‑1 (MQ=255)                                                                                                                                  ccctggCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTATAAAGCTTTGCGAGGGAACATGCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTAttt                                                                                                                <  1:538903/246‑1 (MQ=255)                                                                                                                                   ccATGGCGGTCCGACACGAAAATGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCACACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAAAGCCGGCCAGGTTGCCCCCACCCACGCGCGCCGCA‑AGACTGGGACA‑CAAAGACTGAAATGTGGGTTAACCGCCCGGATGTG‑GGTGAATGCTAtttt                                                                                                               >  2:619826/1‑251 (MQ=255)                                                                                                                                     aTGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GGTGAATGCTAttttt                                                                                                              >  1:43479/1‑250 (MQ=255)                                                                                                                                     aTGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATTATAtttttt                                                                                                             >  1:94733/1‑250 (MQ=255)                                                                                                                                        ccGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCACGCAACGCCGGCCAGGGTGCCGCCACCCACGCGCGCCGCA‑AGACTGGTACA‑CAACGAATGAACTGAGGGTAAACCGCCTGGCT‑TGTGATGAATGCTATTTTTAAgc                                                                                                          >  2:520448/1‑250 (MQ=255)                                                                                                                                           gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTGATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACAACAA‑GAATTAAATGACGGTAAACCGCCTGGCTGGG‑GATGGATGCTATTTTTAAGACtt                                                                                                       >  1:125506/1‑251 (MQ=255)                                                                                                                                             acGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAAAATTCGGCAAACGAGGACGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCCGCGCCACGGCGGCAAGGATGCCCCTACCCACCCGCGCCGCACGGCCTGG‑ACA‑CCATGAATGCAATGAGGTTAAACCGCCTGTCTGTG‑GGTGAATACTATTTTTAAGACtttt                                                                                                     >  2:390620/2‑251 (MQ=255)                                                                                                                                                    gAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGGTAAACCGCCTGGCTGTG‑GATGAATTCTATTTTTAAGACTTTTGCCAAAc                                                                                              >  1:191775/1‑251 (MQ=255)                                                                                                                                                                 ggcatCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAACGCCGGCCAGGGTGCCGCTAACCCCGCGCCCCGCA‑AGACTGGTACA‑CAAAGACTGAAATGAGGGTAAACCGCCTGGCGGTG‑GGTGAAAGCTATTTTTAAGAATTTTGACAAACTGGCggggtgt                                                                                   >  2:331492/5‑244 (MQ=255)                                                                                                                                                                   catCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGGACA‑CAATGACTGAAATGAGGGTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGAATTTTGCCAAACTGGCGGATGTAGCg                                                                                >  1:217588/3‑250 (MQ=255)                                                                                                                                                                    atCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCC‑AGCCTGGGACA‑CCAAGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAAGGCTATTTTTATGACTTTTTCCAAACAGGCGGGTGTAgagg                                                                               >  2:445052/2‑247 (MQ=255)                                                                                                                                                                                 aaaagctttgctagggaACATTCGGCAAGCGAGGTCTCCATGCCGATAAACGCGGCAACCCACATCCAGAAGCCGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAAGGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATcc                                                                <  1:68935/236‑1 (MQ=255)                                                                                                                                                                                            gagggaACATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCTACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATCCGGTGCG‑AAAAg                                                    <  2:173366/247‑1 (MQ=255)                                                                                                                                                                                                 aaCATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCCCCACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCA‑CGACTGGTACA‑CCATGCCTGAAATGAGGTTAAACCGCCTGGCTGTG‑GGTGAATGCTATTTTTTAGACTTTTGCCCAACTGGCGGGTGGAGCGAAA‑ACGCACAAATTCGGTGCG‑AAAAGTGAAc                                               >  2:110028/1‑251 (MQ=255)                                                                                                                                                                                                     ttCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCC‑AGACTGGGACA‑CAATGGCCGAAATGAGGTTAAACCGCCCGGCTGTG‑GGTGAATGCTATTTTTTAGACTTTTGCCAAACCTGCGGATGGAGCGAAAACT‑CACAAATCCGGGGCG‑GAAAGTGGACcaa                                            >  2:484345/1‑250 (MQ=255)                                                                                                                                                                                                     ttCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGACAGCCCAACGCCGGCCAGGGAGCCGCTACCCCAGCGCGCCGCC‑AGGATGGGACA‑CAATGAATGAAATGAGGATAACCCGCCCGGCTGTG‑GGTGAATGCTCTTTTTTAGACTTTTGCCAAACTGGCGGAAGTAACGAAA‑CTGCCCAAATCCGGTGCG‑AAAAGAGAACcaac                                           >  1:365742/1‑251 (MQ=255)                                                                                                                                                                                                      tCGGAAAACGAGGCCGCCAGGCCGATAAACGCGGCAACCCACACCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAGTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTT‑GATGAATGCTATTTGTAAGACTTGTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATCCGGTGCG‑AAAAGTGAACcaac                                           <  2:592607/250‑1 (MQ=255)                                                                                                                                                                                                             aCGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCCCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTgcg                                   <  2:483979/251‑1 (MQ=255)                                                                                                                                                                                                              cGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTgcgc                                  <  1:182650/251‑1 (MQ=255)                                                                                                                                                                                                                     gCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGGTGCCGCTACCCACGCGCGCCCCC‑AGACTGGTACA‑CAAAGACTGAAATGAGGGTAAACCGCCTGGCTGTG‑GGTGAATGCTATTTTTAAGACTTTTTCCCAACTTGCGGGTGTAACGGAA‑CTTCCCAAAACCGGTGCG‑aaaaagtaacccacaacccggcccgcaca                            >  2:341578/1‑225 (MQ=255)                                                                                                                                                                                                                                aaaCGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATc                 <  1:68324/250‑1 (MQ=255)                                                                                                                                                                                                                                      ggCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGGCGCTACCCCCGCGCGCCGCA‑AGAACGGCACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGGTGTGGCGAAA‑ATGCAAAAATTCGGGGCG‑GAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATTATTACCg          >  1:552189/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaCCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAACGCCCGCCCGGGTGCCGCTACCCACGCGCGCCGCA‑AGACTGGGACA‑CAATGACTGAAATGAGGTTAAACCGCCCGGCTGTG‑GGTGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑ATGCACAAATCCGGTGCG‑AAAAGTGAACCCACAAACTGCGCCGCAGAGCAGGGAAATTATTAACGATc       >  2:346000/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaCCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCAGCCCAACGCCGGCCCGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GGTGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGGTGTAGCGAAA‑CTGCACAAATCCGGTGCGCAAAAG‑GAACCAAACACCTGCGCCGGAAAGCAGGGAAATCATTACCGATc       >  2:35645/1‑251 (MQ=255)                                                                                                                                                                                                                                             cacaTCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCa   <  1:86900/251‑1 (MQ=255)                                                                                                                                                                                                                                                aTCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGGCTTTTGCCAAACTGGCGGATGTAGCGAAA‑ATGCACAAATCCGGGGCG‑AAAAGGGAACCAACAACCTGCGCCGAAGAGCAGGGAAAACATTAACGATCCCCaa  >  1:177502/1‑249 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                         CAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGAAA‑CTGCACAAATCCGGTGCG‑AAAAGTGAACCAACAACCTGCGCCGAAGAGCAGGTAAATCATTACCGATCCCCAA  >  NC_000913/7437‑7924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑