Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	7,627	A→G	S111S (TCT→TCC)	yaaJ ←	putative transporter YaaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	7,627	0	A	G	100.0%	175.6 / NA	56	S111S (TCT→TCC)	yaaJ	putative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/23);  total (33/23)

ATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGA  >  NC_000913/7382‑7849                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  atcgCCAGCAGAGCCAAGACAGCGAGAATAATACCTGTCAACAGCGGGGGAAAATCAAAAGAAAAACTCAGAGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCCTCCCCAGCCCGCGCGCCATATACCATGCAGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTCTAAAGCTGTGCGAGGGAAc                                                                                                                                                                                                                               >  2:249570/4‑250 (MQ=255)          agagCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCCAACAGAACGCCCATCCAGCGCATCCCCATCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGTCATCACGTCCTTTATAAAGCTGTGCTAGGGATCATTCGGCaaa                                                                                                                                                                                                                     <  1:484529/251‑1 (MQ=255)           gagcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAAc                                                                                                                                                                                                                    >  1:592607/5‑251 (MQ=255)            agcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGATCGCGAAACGGAGTTCGCCTGAATTCCGCTGATAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGACCGCGCGCCAAATACCAGGCCGGACCGCCACGAAACTGCCCATTGGCATCACGTTGTTTATAAAGCTGTGCGCGGGAACATTCAGCAAACg                                                                                                                                                                                                                   >  1:277259/4‑251 (MQ=255)            agcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACGCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACg                                                                                                                                                                                                                   <  1:105825/248‑1 (MQ=255)                 aGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTc                                                                                                                                                                                                              <  2:200336/251‑1 (MQ=255)                    cAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGcc                                                                                                                                                                                                           >  1:137501/1‑251 (MQ=255)                             aaataccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATa                                                                                                                                                                                                  <  1:370898/246‑1 (MQ=255)                              aataccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCAGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGCCCGCCACGAAACTGCCCCTTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATa                                                                                                                                                                                                  >  2:555721/5‑250 (MQ=255)                                  ccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACGCAGGGCGCGCGCAACGGCGTGCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAcgc                                                                                                                                                                                             <  1:319605/251‑1 (MQ=255)                                   cTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCACCGGCGTTAGCCTGAATTCCGCTGCAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGAATCCCCAGCCCGCGCGCCATATACCATGCCGGTACGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAcgcg                                                                                                                                                                                            >  2:607514/1‑251 (MQ=255)                                    tGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGACGCCATGCCGATAAACGCgg                                                                                                                                                                                           >  1:451862/1‑251 (MQ=255)                                                  gAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGACCATCCAGCGAATCCCCAGCCCGCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATcc                                                                                                                                                                               >  1:583644/1‑249 (MQ=255)                                                   aaaaTAAAAAGAAAAACCCAGGGCTCGCGCAACGGCGTTCACCTGAATTCCGCGGAAAATTAGGCCGTAGGCGATGGGCAAAAAGACGGCGAACAGAACGCCCATCCAGCCCATCCCCAGCCCGCGCGCCTTATACCATGCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGCCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCaga                                                                                                                                                                            <  2:519143/251‑3 (MQ=255)                                                        cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg                                                                                                                                                                       <  1:232223/251‑1 (MQ=255)                                                         aaaaGTAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACgg                                                                                                                                                                      <  1:620114/251‑1 (MQ=255)                                                             aaaaaaCCCAGGGCTCGGCCAACGGCTTTCGCCTGAATGCCGCTGAAAATGATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCTCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGTTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAATACGGCTcc                                                                                                                                                                  <  2:288932/250‑1 (MQ=255)                                                                aaaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAgg                                                                                                                                                               >  1:483979/1‑251 (MQ=255)                                                                aaaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCAAgg                                                                                                                                                               >  2:260682/1‑251 (MQ=255)                                                                 aaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGt                                                                                                                                                              >  2:548345/1‑251 (MQ=255)                                                                  aCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTc                                                                                                                                                             >  2:86900/1‑251 (MQ=255)                                                                     cAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTcc                                                                                                                                                            <  1:18816/249‑1 (MQ=255)                                                                            ggcgcagcGGCGTTCGCCTGAATCCCGCTAAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCATCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAATCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcg                                                                                                                                                     <  2:195630/242‑1 (MQ=255)                                                                                          cgcctGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCAGCGCac                                                                                                                                     >  2:279800/5‑250 (MQ=255)                                                                                                     cGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGAAATTGACAGCGCAACGCCGGCCAgg                                                                                                                          >  1:463767/1‑251 (MQ=255)                                                                                                       cTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACACCGGCCAGGtt                                                                                                                        >  1:87187/1‑251 (MQ=255)                                                                                                         gAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                                                        <  1:531352/249‑1 (MQ=255)                                                                                                                   ccgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGTTCCCGcgccccac                                                                                                            >  2:350145/4‑244 (MQ=255)                                                                                                                        ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCACGCGCGCCATATACCATGCCGGTCCGCCGCGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGCGGGAACATTCGGCAAACGAGGTCGCCATGCCGCTAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCAGCGGAACGCCGGCCCGGTTGACGGTACCCAcgcgac                                                                                                       >  1:362641/1‑249 (MQ=255)                                                                                                                         gCGATGAGCAAAACGACGGCGAACAGAACGCCAATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGGTGCCGCCACCCACccgcgc                                                                                                      >  1:499886/1‑251 (MQ=255)                                                                                                                             tGAGCAAAACGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGACGGCCAGGGTTCCGCCACCCACGGGcgacgc                                                                                                   >  1:418753/1‑250 (MQ=255)                                                                                                                                       gACGGCGAACATACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTac                                                                                       <  1:249570/251‑1 (MQ=255)                                                                                                                                        aCGGCGAACAGCACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACACCGGCCAGGGTGCCGCTACCCCCGCGcgccgccacacagggacc                                                                                       >  2:450849/1‑239 (MQ=255)                                                                                                                                         cGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTaca‑c                                                                                    <  1:440603/251‑1 (MQ=255)                                                                                                                                           gCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑Caa                                                                                  <  1:607514/251‑1 (MQ=255)                                                                                                                                             gAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAtt                                                                                                                                               <  1:172086/190‑2 (MQ=255)                                                                                                                                                            ccAGCGCATCCCCAGCCCGCGCGCCATATCCCTGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTa                                                                 <  2:159119/251‑1 (MQ=255)                                                                                                                                                             cAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGACATGAgggta                                                                 >  1:79965/1‑247 (MQ=255)                                                                                                                                                                cgcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAAcc                                                             <  1:623283/251‑1 (MQ=255)                                                                                                                                                                 gcATCCCCAGCCCGCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCg                                                            <  1:383276/251‑1 (MQ=255)                                                                                                                                                                 gcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGTTGCCGCTACCCCCGCGCGCCTCA‑AGACTGGGACA‑CACAGACTGAAATGAGGTTAAAccc                                                            >  1:114212/1‑250 (MQ=255)                                                                                                                                                                     ccccAGCCCCCGCGCCATAGCCCATGCCGGTCCGCCACGAAACTGCCCATTTGCACCCCTTTCTTTATAAAGCTGGGCGAGGGAACATTCGGCAAACGAGGTCGCCAGGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTg                                                        <  2:463767/251‑1 (MQ=255)                                                                                                                                                                                cccccaaaacccATGCCGGCCCGCCCCGAAACGGCCCATTGGCATCACTTTCTTGATAAAGCTGTGCGAGGGAACATTCGGAAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGa                                            <  2:548305/241‑1 (MQ=255)                                                                                                                                                                                      tataCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCCGCGCCACGACGGCCCGGTTGCCGCTCCCCACGCCCGCCCCC‑CGCGTGGGACA‑CAATGACTTGAATGAGGGTAAAcccgccgggtgggggggcatgatt                                       >  2:288852/1‑229 (MQ=255)                                                                                                                                                                                        aaCCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACGGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCGGTG‑GAGGAATGCTAtt                                    <  2:116635/250‑1 (MQ=255)                                                                                                                                                                                         ccctggCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTATAAAGCTTTGCGAGGGAACATGCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTAttt                                   <  1:538903/246‑1 (MQ=255)                                                                                                                                                                                          ccATGGCGGTCCGACACGAAAATGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCACACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAAAGCCGGCCAGGTTGCCCCCACCCACGCGCGCCGCA‑AGACTGGGACA‑CAAAGACTGAAATGTGGGTTAACCGCCCGGATGTG‑GGTGAATGCTAtttt                                  >  2:619826/1‑251 (MQ=255)                                                                                                                                                                                            aTGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GGTGAATGCTAttttt                                 >  1:43479/1‑250 (MQ=255)                                                                                                                                                                                            aTGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATTATAtttttt                                >  1:94733/1‑250 (MQ=255)                                                                                                                                                                                               ccGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCACGCAACGCCGGCCAGGGTGCCGCCACCCACGCGCGCCGCA‑AGACTGGTACA‑CAACGAATGAACTGAGGGTAAACCGCCTGGCT‑TGTGATGAATGCTATTTTTAAgc                             >  2:520448/1‑250 (MQ=255)                                                                                                                                                                                                  gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTGATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACAACAA‑GAATTAAATGACGGTAAACCGCCTGGCTGGG‑GATGGATGCTATTTTTAAGACtt                          >  1:125506/1‑251 (MQ=255)                                                                                                                                                                                                    acGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAAAATTCGGCAAACGAGGACGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCCGCGCCACGGCGGCAAGGATGCCCCTACCCACCCGCGCCGCACGGCCTGG‑ACA‑CCATGAATGCAATGAGGTTAAACCGCCTGTCTGTG‑GGTGAATACTATTTTTAAGACtttt                        >  2:390620/2‑251 (MQ=255)                                                                                                                                                                                                           gAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGGTAAACCGCCTGGCTGTG‑GATGAATTCTATTTTTAAGACTTTTGCCAAAc                 >  1:191775/1‑251 (MQ=255)                                                                                                                                                                                                                        ggcatCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAACGCCGGCCAGGGTGCCGCTAACCCCGCGCCCCGCA‑AGACTGGTACA‑CAAAGACTGAAATGAGGGTAAACCGCCTGGCGGTG‑GGTGAAAGCTATTTTTAAGAATTTTGACAAACTGGCggggtgt      >  2:331492/5‑244 (MQ=255)                                                                                                                                                                                                                          catCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGGACA‑CAATGACTGAAATGAGGGTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGAATTTTGCCAAACTGGCGGATGTAGCg   >  1:217588/3‑250 (MQ=255)                                                                                                                                                                                                                           atCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCC‑AGCCTGGGACA‑CCAAGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAAGGCTATTTTTATGACTTTTTCCAAACAGGCGGGTGTAgagg  >  2:445052/2‑247 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  ATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGA  >  NC_000913/7382‑7849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑