Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,567	A→G	E130E (GAA→GAG)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,567	0	A	G	100.0%	132.9 / NA	45	E130E (GAA→GAG)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/24);  total (21/24)

AAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGT  >  NC_000913/4638337‑4638809                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    aaaCTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCat                                                                                                                                                                                                                                 >  1:196988/1‑250 (MQ=255)    cTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCtt                                                                                                                                                                                                                              >  1:177790/1‑251 (MQ=255)                cttaTCGCTATTCCTGTGACCGAACTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGCAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGccctt                                                                                                                                                                                                                  >  2:227906/4‑248 (MQ=255)                  taTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCttt                                                                                                                                                                                                                 <  2:161447/249‑1 (MQ=255)                     cGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTAtt                                                                                                                                                                                                              <  2:177790/250‑1 (MQ=255)                     cGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCACTTTAtt                                                                                                                                                                                                              <  2:488695/250‑1 (MQ=255)                      gCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGCGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATtgt                                                                                                                                                                                                            >  2:370931/1‑251 (MQ=255)                       gtATTCCTGTGTCCGAGCTTTATACGGTGCAGGAAGATGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGAGGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATTCCGGTCAGGTCTGGCACAGTGAATTAACGCTAAAATCCGATTTCGGTGTTTCGCGTCTTGGCGAGTTTAACGCGGCAAGTATCatctgagccaatccctgtattgtg                                                                                                                                                                                                           <  2:37434/250‑24 (MQ=255)                           tCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGAtt                                                                                                                                                                                                        <  2:18549/250‑1 (MQ=255)                                         tttATACGGTGCAGGACGAGGATAAAACCGTGTAGCGGAACCGCTGTTTTATCCAATTTTGGTTAACTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGACATTTATTGTGATTCGCGTCGGGGATgcg                                                                                                                                                                                         >  2:213149/1‑251 (MQ=255)                                             taCGGTGCAGGCAGAGGATAAAACCGTGGAGAGGAAACGAAGTTTTCTCCATTTTTGGTTACCTGAGTCATTGCTGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGATAAGGACTGGCAAAGTGATTTAACGCTCAAAGCCGATTTCACTGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCCAGCCCTTTCTTGAGATTAGCGTCGGGAATGCGCGTg                                                                                                                                                                                     >  2:572509/1‑251 (MQ=255)                                                          gagtaTAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑tggtgtggt                                                                                                                                                                       <  1:126207/247‑1 (MQ=255)                                                                      gTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGa                                                                                                                                                           <  1:263800/251‑1 (MQ=255)                                                                         gAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCTATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCTTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGt                                                                                                                                                        <  2:496634/251‑1 (MQ=255)                                                                                 aCGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAgc                                                                                                                                                                 <  2:506901/234‑1 (MQ=255)                                                                                 aCGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAgc                                                                                                                                                                 >  1:506901/1‑234 (MQ=255)                                                                                   gAAGTTTTATCCATTTTTCGTTACCTGAGCCATTGATGCTTGATGACAATCAGCACGTGGACGAACGCAAGCTAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTCT‑TGGTGTGGGGAAAGCGCCTGAAGTTaacggaccg                                                                                                                                              >  2:93339/1‑248 (MQ=255)                                                                                          tATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTg                                                                                                                                        <  1:468220/250‑1 (MQ=255)                                                                                            tCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGAcc                                                                                                                                     >  1:130359/1‑251 (MQ=255)                                                                                               aTTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAGTCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCAtt                                                                                                                                  <  2:113067/251‑1 (MQ=255)                                                                                                 ttttGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGAGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGAGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTg                                                                                                                                 >  1:362060/1‑250 (MQ=255)                                                                                                    tGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAcc                                                                                                                             >  1:337832/1‑251 (MQ=255)                                                                                                    tGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAc                                                                                                                              <  1:107097/250‑1 (MQ=255)                                                                                                      gTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCg                                                                                                                           <  1:308262/251‑1 (MQ=255)                                                                                                         aCCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTATGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAt‑t                                                                                                                                                                               <  1:383953/196‑1 (MQ=255)                                                                                                         aCCTGAGTAATTGATGGTTGATGGCAATCAGCACGTGGCAGAACGCAAGATAGGGCTTTATACCGGTCAGGTCTGGCACAGAGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCACATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGGAt‑t                                                                                                                                                                               >  2:383953/1‑196 (MQ=255)                                                                                                                      atggttgatggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTTATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGGGGTGAAAGCGACTGAAGTTAACGGAACGGCGCt                                                                                                                                         <  1:203069/221‑1 (MQ=255)                                                                                                                                   aTCAGAACATGGAAGAACGCAAGATAGGGCTTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAACGCCGCTTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGAAAGGCGGGCAGGGc                                                                                              >  1:229075/1‑251 (MQ=255)                                                                                                                                          cGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCata                                                                                       <  2:448762/251‑1 (MQ=255)                                                                                                                                             ggAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTCGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGCTAACGGAACGGCGCGGGCACTTGAACCCGGCACCGGGTTAGAGCACGGCGGACCGGGAGTGCatat                                                                                      >  2:598920/1‑249 (MQ=255)                                                                                                                                             ggAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTGTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGGCCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCatat                                                                                      <  1:598920/249‑1 (MQ=255)                                                                                                                                                agaaCGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCtt                                                                                 <  1:511508/251‑1 (MQ=255)                                                                                                                                                   actcAAGATAGTGACTTGTACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATGTCGATGGTTCGCGTCTTAGCTAGCGCAACGCGCCAAAGATCATCTTAGGCAAGCCCTTTAGTGTGATTGTCGTCGGGGATTCGCGTTGTAC‑TGGTGTTGTGTAAGCGCCTGATGTGAACGGAACGTCGCTGACCATTGAACCCCGCACCTGGTTAGAGCAAGGCTGGCTTGTCGTGCATAGCCCTTTAc                                                                              <  2:50620/248‑1 (MQ=255)                                                                                                                                                          aTAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGGACCGGGTTAGAGCAAGTCGGGCAGGGCGTGCATATCCCGTTACCCGAAgg                                                                       <  2:82772/251‑1 (MQ=255)                                                                                                                                                             gggATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGa                                                                    <  1:302400/251‑1 (MQ=255)                                                                                                                                                                   tataCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGGAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCGGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGc                                                               <  1:227906/250‑1 (MQ=255)                                                                                                                                                                         ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTAGATGTTTCACGTCTTAGCGAGCTAAACGCGCCAAATATCATCTTAGGCCAGCCCTTTATTGTGATTAGCGACGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCAGAAAGGGACTGGAGGaacc                                                         >  1:290543/1‑248 (MQ=255)                                                                                                                                                                                      acagtgagttcacgctatacgCCGATTTCGATGTTTCGCGTCTTACCGAGCTCAACGCCCCAAATATCATATTAGTCACGCCCTTTATTGTGATTAGCGTCGGGGCTGCGCGTGGTAT‑TGGCGTGGTGATAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAACAGACGCTGAAGCTg                                           >  2:95029/21‑251 (MQ=255)                                                                                                                                                                                                aaCGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGccc                                 >  1:431291/1‑251 (MQ=255)                                                                                                                                                                                                        aaGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTCT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGCACGGCGCTGACCCTTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGAGTGCATATACCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTAAAGCTTAATATGGCCCTGAAtttt                         >  2:557990/1‑250 (MQ=255)                                                                                                                                                                                                         aGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGCACGGCGCTGACCATTGAACCCGGCACCGGGGTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAGGGGGACTGGCGGAAGCAGAACCTGGAGccggataatgccctgtatata                         >  2:110076/1‑230 (MQ=255)                                                                                                                                                                                                                  cGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTAcc                <  2:345456/250‑1 (MQ=255)                                                                                                                                                                                                                   gATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCACGCCCTTTATTGTGATTAGCGTCAGGGATGCGCGTGGCAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCGGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCCGGGCGTGCATATCCCTTTACCCGAAGGGGCCTGGCGGAAGCAGAAACTGAAGCTGGATATGGCCCTGAATTTAAGCGGTGCCgg              >  1:234160/1‑251 (MQ=255)                                                                                                                                                                                                                             ggctgaGCGAGCTCAACGCGGCAAATATCATCTTAGGCAAGCCCTTTAGTGTGATTAGCGTCGGGGATGCGCGTGTTATGTGG‑GTGGTGAAAGCGCCAGAAGTTAACGGAACGGCGCTGACCATTGAGCCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGGATATCCCGTGATCCGAAGGGGTCTGTCGGAGGCAGAGCCTGAAGCTCAATATGGGCCTGAATGTGAGCGGTCCGGGCGCTCTTTCt    <  2:538537/246‑1 (MQ=255)                                                                                                                                                                                                                               cTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCCTTGAACCCGGCACCGGGGTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGGAGCAGAACCTGAAACTGAATAATGCCCTGAATTTAAAGGGTACACGCGATCCTTCtgg  >  2:354458/1‑250 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    AAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAT‑TGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCCTGAATTTAAGCGGTACCGGCGATCTTTCTGT  >  NC_000913/4638337‑4638809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑