Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,532	T→C	L119L (TTA→CTA)	creD →	putative inner membrane protein CreD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,532	0	T	C	97.8%	134.1 / ‑5.6	45	L119L (TTA→CTA)	creD	putative inner membrane protein CreD
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (20/24);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

TAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCC  >  NC_000913/4638291‑4638778                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       tAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCCAGATAGGGATTTATACCGGTCAGGTCTGGCACCGTGATTTAACGCTAAAAGCCg                                                                                                                                                                                                                                               >  1:582033/1‑251 (MQ=255)    cGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGAtt                                                                                                                                                                                                                                            >  2:308262/1‑251 (MQ=255)      aTGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGCCTGGCACAGTGATTTAACGCTAAAAGCCGATTTc                                                                                                                                                                                                                                          >  1:135426/1‑251 (MQ=255)           gAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGt                                                                                                                                                                                                                                     <  1:315329/251‑1 (MQ=255)                   aaTTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGCGCTTTATACGGAGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCAGATTTCGATGTTTCGCGTc                                                                                                                                                                                                                             >  2:174587/2‑251 (MQ=255)                     ttCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCtt                                                                                                                                                                                                                           >  1:587707/1‑251 (MQ=255)                             aGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGAGGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATTCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGagct                                                                                                                                                                                                                   <  1:76957/251‑4 (MQ=255)                                               aaaCTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCat                                                                                                                                                                                                 >  1:196988/1‑250 (MQ=255)                                                  cTCGTTGGGCCGCTTATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCtt                                                                                                                                                                                              >  1:177790/1‑251 (MQ=255)                                                              cttaTCGCTATTCCTGTGACCGAACTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGCAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGccctt                                                                                                                                                                                  >  2:227906/4‑248 (MQ=255)                                                                taTCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCttt                                                                                                                                                                                 <  2:161447/249‑1 (MQ=255)                                                                   cGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTAtt                                                                                                                                                                              <  2:177790/250‑1 (MQ=255)                                                                   cGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCACTTTAtt                                                                                                                                                                              <  2:488695/250‑1 (MQ=255)                                                                    gCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGCGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATtgt                                                                                                                                                                            >  2:370931/1‑251 (MQ=255)                                                                     gtATTCCTGTGTCCGAGCTTTATACGGTGCAGGAAGATGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGAGGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATTCCGGTCAGGTCTGGCACAGTGAATTAACGCTAAAATCCGATTTCGGTGTTTCGCGTCTTGGCGAGTTTAACGCGGCAAGTATCatctgagccaatccctgtattgtg                                                                                                                                                                           <  2:37434/250‑24 (MQ=255)                                                                         tCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGAtt                                                                                                                                                                        <  2:18549/250‑1 (MQ=255)                                                                                       tttATACGGTGCAGGACGAGGATAAAACCGTGTAGCGGAACCGCTGTTTTATCCAATTTTGGTTAACTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGACATTTATTGTGATTCGCGTCGGGGATgcg                                                                                                                                                         >  2:213149/1‑251 (MQ=255)                                                                                           taCGGTGCAGGCAGAGGATAAAACCGTGGAGAGGAAACGAAGTTTTCTCCATTTTTGGTTACCTGAGTCATTGCTGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGATAAGGACTGGCAAAGTGATTTAACGCTCAAAGCCGATTTCACTGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCCAGCCCTTTCTTGAGATTAGCGTCGGGAATGCGCGTg                                                                                                                                                     >  2:572509/1‑251 (MQ=255)                                                                                                        gagtaTAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATtggtgtggt                                                                                                                                        <  1:126207/247‑1 (MQ=255)                                                                                                                    gTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGa                                                                                                                            <  1:263800/251‑1 (MQ=255)                                                                                                                       gAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCTATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCTTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGt                                                                                                                         <  2:496634/251‑1 (MQ=255)                                                                                                                               aCGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAgc                                                                                                                                  <  2:506901/234‑1 (MQ=255)                                                                                                                               aCGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAgc                                                                                                                                  >  1:506901/1‑234 (MQ=255)                                                                                                                                 gAAGTTTTATCCATTTTTCGTTACCTGAGCCATTGATGCTTGATGACAATCAGCACGTGGACGAACGCAAGCTAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTCTTGGTGTGGGGAAAGCGCCTGAAGTTaacggaccg                                                                                                               >  2:93339/1‑248 (MQ=255)                                                                                                                                        tATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTg                                                                                                         <  1:468220/250‑1 (MQ=255)                                                                                                                                          tCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGAcc                                                                                                      >  1:130359/1‑251 (MQ=255)                                                                                                                                             aTTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAGTCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCAtt                                                                                                   <  2:113067/251‑1 (MQ=255)                                                                                                                                               ttttGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGAGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGAGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTg                                                                                                  >  1:362060/1‑250 (MQ=255)                                                                                                                                                  tGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAcc                                                                                              >  1:337832/1‑251 (MQ=255)                                                                                                                                                  tGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAAc                                                                                               <  1:107097/250‑1 (MQ=255)                                                                                                                                                    gTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCg                                                                                            <  1:308262/251‑1 (MQ=255)                                                                                                                                                       aCCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTATGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTAtt                                                                                                                                                <  1:383953/196‑1 (MQ=255)                                                                                                                                                       aCCTGAGTAATTGATGGTTGATGGCAATCAGCACGTGGCAGAACGCAAGATAGGGCTTTATACCGGTCAGGTCTGGCACAGAGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCACATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGGAtt                                                                                                                                                >  2:383953/1‑196 (MQ=255)                                                                                                                                                                    atggttgatggCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTTATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGGGGTGAAAGCGACTGAAGTTAACGGAACGGCGCt                                                                                                          <  1:203069/221‑1 (MQ=255)                                                                                                                                                                                 aTCAGAACATGGAAGAACGCAAGATAGGGCTTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAACGCCGCTTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGAAAGGCGGGCAGGGc                                                               >  1:229075/1‑251 (MQ=255)                                                                                                                                                                                        cGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCata                                                        <  2:448762/251‑1 (MQ=255)                                                                                                                                                                                           ggAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTCGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGCTAACGGAACGGCGCGGGCACTTGAACCCGGCACCGGGTTAGAGCACGGCGGACCGGGAGTGCatat                                                       >  2:598920/1‑249 (MQ=255)                                                                                                                                                                                           ggAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTGTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGGCCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCatat                                                       <  1:598920/249‑1 (MQ=255)                                                                                                                                                                                              agaaCGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCtt                                                  <  1:511508/251‑1 (MQ=255)                                                                                                                                                                                                 actcAAGATAGTGACTTGTACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATGTCGATGGTTCGCGTCTTAGCTAGCGCAACGCGCCAAAGATCATCTTAGGCAAGCCCTTTAGTGTGATTGTCGTCGGGGATTCGCGTTGTACTGGTGTTGTGTAAGCGCCTGATGTGAACGGAACGTCGCTGACCATTGAACCCCGCACCTGGTTAGAGCAAGGCTGGCTTGTCGTGCATAGCCCTTTAc                                               <  2:50620/248‑1 (MQ=255)                                                                                                                                                                                                        aTAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGGACCGGGTTAGAGCAAGTCGGGCAGGGCGTGCATATCCCGTTACCCGAAgg                                        <  2:82772/251‑1 (MQ=255)                                                                                                                                                                                                           gggATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGa                                     <  1:302400/251‑1 (MQ=255)                                                                                                                                                                                                                 tataCCGGTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGGATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCGGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGc                                <  1:227906/250‑1 (MQ=255)                                                                                                                                                                                                                       ggTCAGGTCTGGCACAGTGATTTAACGCTAAAAGCCGATTTAGATGTTTCACGTCTTAGCGAGCTAAACGCGCCAAATATCATCTTAGGCCAGCCCTTTATTGTGATTAGCGACGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCAGAAAGGGACTGGAGGaacc                          >  1:290543/1‑248 (MQ=255)                                                                                                                                                                                                                                              aaCGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAGCTCAACGCGCCAAATATCATCTTAGGCAAGCCCTTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCCGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGccc  >  1:431291/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       TAGCGATGTGGAAGATGCGATTCGCCAAAGTACCAGCGGGCCGCAAAAACTCGTTGGGCCGCTCATCGCTATTCCTGTGACCGAGCTTTATACGGTGCAGGAAGAGGATAAAACCGTGGAGCGGAAACGAAGTTTTATCCATTTTTGGTTACCTGAGTCATTGATGGTTGATGGCAATCAGAACGTGGAAGAACGCAAGATAGGGATTTATACCGGTCAGGTCTGGCACAGTGATTTAACGTTAAAAGCCGATTTCGATGTTTCGCGTCTTAGCGAACTCAACGCGCCAAATATCACCTTAGGCAAGCCATTTATTGTGATTAGCGTCGGGGATGCGCGTGGTATTGGTGTGGTGAAAGCGCCTGAAGTTAACGGAACGGCGCTGACCATTGAACCCGGCACCGGGTTAGAGCAAGGCGGGCAGGGCGTGCATATCCCTTTACCTGAAGGGGACTGGCGGAAGCAGAACCTGAAGCTGAATATGGCCC  >  NC_000913/4638291‑4638778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑