Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 7,624 C→G L112L (CTG→CTC)  yaaJ ← putative transporter YaaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009137,6240CG98.2% 174.8 / ‑5.4 56L112L (CTG→CTC) yaaJputative transporter YaaJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (33/22);  minor base T (0/1);  total (33/23)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

ATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGA  >  NC_000913/7382‑7849
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    
atcgCCAGCAGAGCCAAGACAGCGAGAATAATACCTGTCAACAGCGGGGGAAAATCAAAAGAAAAACTCAGAGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGCTGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCCTCCCCAGCCCGCGCGCCATATACCATGCAGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTCTAAAGCTGTGCGAGGGAAc                                                                                                                                                                                                                               >  2:249570/4‑250 (MQ=255)
         agagCAAAGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCCAACAGAACGCCCATCCAGCGCATCCCCATCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGTCATCACGTCCTTTATAAAGCTGTGCTAGGGATCATTCGGCaaa                                                                                                                                                                                                                     <  1:484529/251‑1 (MQ=255)
          gagcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAAc                                                                                                                                                                                                                    >  1:592607/5‑251 (MQ=255)
           agcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGATCGCGAAACGGAGTTCGCCTGAATTCCGCTGATAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGACCGCGCGCCAAATACCAGGCCGGACCGCCACGAAACTGCCCATTGGCATCACGTTGTTTATAAAGCTGTGCGCGGGAACATTCAGCAAACg                                                                                                                                                                                                                   >  1:277259/4‑251 (MQ=255)
           agcaaaGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACGCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACg                                                                                                                                                                                                                   <  1:105825/248‑1 (MQ=255)
                aGACAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTc                                                                                                                                                                                                              <  2:200336/251‑1 (MQ=255)
                   cAGCGAGAATAATACCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGcc                                                                                                                                                                                                           >  1:137501/1‑251 (MQ=255)
                            aaataccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATa                                                                                                                                                                                                  <  1:370898/246‑1 (MQ=255)
                             aataccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCAGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGCCCGCCACGAAACTGCCCCTTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATa                                                                                                                                                                                                  >  2:555721/5‑250 (MQ=255)
                                 ccTGTCACCAGCGGGGGAAAATCAAAAGAAAAACGCAGGGCGCGCGCAACGGCGTGCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAcgc                                                                                                                                                                                             <  1:319605/251‑1 (MQ=255)
                                  cTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCACCGGCGTTAGCCTGAATTCCGCTGCAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGAATCCCCAGCCCGCGCGCCATATACCATGCCGGTACGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAAcgcg                                                                                                                                                                                            >  2:607514/1‑251 (MQ=255)
                                   tGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGACGCCATGCCGATAAACGCgg                                                                                                                                                                                           >  1:451862/1‑251 (MQ=255)
                                                 gAAAATCAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGACCATCCAGCGAATCCCCAGCCCGCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATcc                                                                                                                                                                               >  1:583644/1‑249 (MQ=255)
                                                  aaaaTAAAAAGAAAAACCCAGGGCTCGCGCAACGGCGTTCACCTGAATTCCGCGGAAAATTAGGCCGTAGGCGATGGGCAAAAAGACGGCGAACAGAACGCCCATCCAGCCCATCCCCAGCCCGCGCGCCTTATACCATGCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGCCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCaga                                                                                                                                                                            <  2:519143/251‑3 (MQ=255)
                                                       cAAAAGAAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACg                                                                                                                                                                       <  1:232223/251‑1 (MQ=255)
                                                        aaaaGTAAAACTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACgg                                                                                                                                                                      <  1:620114/251‑1 (MQ=255)
                                                            aaaaaaCCCAGGGCTCGGCCAACGGCTTTCGCCTGAATGCCGCTGAAAATGATGCCGGAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCTCGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGTTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAATACGGCTcc                                                                                                                                                                  <  2:288932/250‑1 (MQ=255)
                                                               aaaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAgg                                                                                                                                                               >  1:483979/1‑251 (MQ=255)
                                                               aaaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCAAgg                                                                                                                                                               >  2:260682/1‑251 (MQ=255)
                                                                aaCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGt                                                                                                                                                              >  2:548345/1‑251 (MQ=255)
                                                                 aCTCAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTc                                                                                                                                                             >  2:86900/1‑251 (MQ=255)
                                                                    cAGGGCTCGCGCAACGGCGTTCGCCTGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTcc                                                                                                                                                            <  1:18816/249‑1 (MQ=255)
                                                                           ggcgcagcGGCGTTCGCCTGAATCCCGCTAAAAATTAGGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCATCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAATCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACcggcg                                                                                                                                                     <  2:195630/242‑1 (MQ=255)
                                                                                         cgcctGAATTCCGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCAGCGCac                                                                                                                                     >  2:279800/5‑250 (MQ=255)
                                                                                                    cGCTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGGCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGAAATTGACAGCGCAACGCCGGCCAgg                                                                                                                          >  1:463767/1‑251 (MQ=255)
                                                                                                      cTGAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACACCGGCCAGGtt                                                                                                                        >  1:87187/1‑251 (MQ=255)
                                                                                                        gAAAATTATGCCGTAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGtt                                                                                                                        <  1:531352/249‑1 (MQ=255)
                                                                                                                  ccgtAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGTTCCCGcgccccac                                                                                                            >  2:350145/4‑244 (MQ=255)
                                                                                                                       ggCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCACGCGCGCCATATACCATGCCGGTCCGCCGCGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGCGGGAACATTCGGCAAACGAGGTCGCCATGCCGCTAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCAGCGGAACGCCGGCCCGGTTGACGGTACCCAcgcgac                                                                                                       >  1:362641/1‑249 (MQ=255)
                                                                                                                        gCGATGAGCAAAACGACGGCGAACAGAACGCCAATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGGTGCCGCCACCCACccgcgc                                                                                                      >  1:499886/1‑251 (MQ=255)
                                                                                                                            tGAGCAAAACGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGACGGCCAGGGTTCCGCCACCCACGGGcgacgc                                                                                                   >  1:418753/1‑250 (MQ=255)
                                                                                                                                      gACGGCGAACATACCGCCCATCCAGCGCATCCCCAGCCCGCGCGCAATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTac                                                                                       <  1:249570/251‑1 (MQ=255)
                                                                                                                                       aCGGCGAACAGCACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACACCGGCCAGGGTGCCGCTACCCCCGCGcgccgccacacagggacc                                                                                       >  2:450849/1‑239 (MQ=255)
                                                                                                                                        cGGCGAACAGACCGCCCACCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTaca‑c                                                                                    <  1:440603/251‑1 (MQ=255)
                                                                                                                                          gCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑Caa                                                                                  <  1:607514/251‑1 (MQ=255)
                                                                                                                                            gAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAtt                                                                                                                                               <  1:172086/190‑2 (MQ=255)
                                                                                                                                                           ccAGCGCATCCCCAGCCCGCGCGCCATATCCCTGGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTa                                                                 <  2:159119/251‑1 (MQ=255)
                                                                                                                                                            cAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGGTGCCGCTACCCCCGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGACATGAgggta                                                                 >  1:79965/1‑247 (MQ=255)
                                                                                                                                                               cgcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAAcc                                                             <  1:623283/251‑1 (MQ=255)
                                                                                                                                                                gcATCCCCAGCCCGCGCGCCATATCCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCg                                                            <  1:383276/251‑1 (MQ=255)
                                                                                                                                                                gcATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCCGGTTGCCGCTACCCCCGCGCGCCTCA‑AGACTGGGACA‑CACAGACTGAAATGAGGTTAAAccc                                                            >  1:114212/1‑250 (MQ=255)
                                                                                                                                                                    ccccAGCCCCCGCGCCATAGCCCATGCCGGTCCGCCACGAAACTGCCCATTTGCACCCCTTTCTTTATAAAGCTGGGCGAGGGAACATTCGGCAAACGAGGTCGCCAGGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTg                                                        <  2:463767/251‑1 (MQ=255)
                                                                                                                                                                               cccccaaaacccATGCCGGCCCGCCCCGAAACGGCCCATTGGCATCACTTTCTTGATAAAGCTGTGCGAGGGAACATTCGGAAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGa                                            <  2:548305/241‑1 (MQ=255)
                                                                                                                                                                                     tataCCATGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGGAATTGCCCGCGCCACGACGGCCCGGTTGCCGCTCCCCACGCCCGCCCCC‑CGCGTGGGACA‑CAATGACTTGAATGAGGGTAAAcccgccgggtgggggggcatgatt                                       >  2:288852/1‑229 (MQ=255)
                                                                                                                                                                                       aaCCAGGCCGGTCCGCCACGAAACTGCCCATTGGCATCCCGTTCTTTATAAAGCTGTGCGAGGGAACATCCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACGGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCGGTG‑GAGGAATGCTAtt                                    <  2:116635/250‑1 (MQ=255)
                                                                                                                                                                                        ccctggCCGGTCCGCCACGAAACGGCCCATTGGCATCACGTTCTTTATAAAGCTTTGCGAGGGAACATGCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTAttt                                   <  1:538903/246‑1 (MQ=255)
                                                                                                                                                                                         ccATGGCGGTCCGACACGAAAATGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCACACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAAAGCCGGCCAGGTTGCCCCCACCCACGCGCGCCGCA‑AGACTGGGACA‑CAAAGACTGAAATGTGGGTTAACCGCCCGGATGTG‑GGTGAATGCTAtttt                                  >  2:619826/1‑251 (MQ=255)
                                                                                                                                                                                           aTGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GGTGAATGCTAttttt                                 >  1:43479/1‑250 (MQ=255)
                                                                                                                                                                                           aTGCCGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATTATAtttttt                                >  1:94733/1‑250 (MQ=255)
                                                                                                                                                                                              ccGGTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCACGCAACGCCGGCCAGGGTGCCGCCACCCACGCGCGCCGCA‑AGACTGGTACA‑CAACGAATGAACTGAGGGTAAACCGCCTGGCT‑TGTGATGAATGCTATTTTTAAgc                             >  2:520448/1‑250 (MQ=255)
                                                                                                                                                                                                 gTCCGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTGATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACAACAA‑GAATTAAATGACGGTAAACCGCCTGGCTGGG‑GATGGATGCTATTTTTAAGACtt                          >  1:125506/1‑251 (MQ=255)
                                                                                                                                                                                                   acGCCACGAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAAAATTCGGCAAACGAGGACGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAAATGCCCGCGCCACGGCGGCAAGGATGCCCCTACCCACCCGCGCCGCACGGCCTGG‑ACA‑CCATGAATGCAATGAGGTTAAACCGCCTGTCTGTG‑GGTGAATACTATTTTTAAGACtttt                        >  2:390620/2‑251 (MQ=255)
                                                                                                                                                                                                          gAAACTGCCCATTGGCATCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCCCGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGGTAAACCGCCTGGCTGTG‑GATGAATTCTATTTTTAAGACTTTTGCCAAAc                 >  1:191775/1‑251 (MQ=255)
                                                                                                                                                                                                                       ggcatCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCCGCGCAACGCCGGCCAGGGTGCCGCTAACCCCGCGCCCCGCA‑AGACTGGTACA‑CAAAGACTGAAATGAGGGTAAACCGCCTGGCGGTG‑GGTGAAAGCTATTTTTAAGAATTTTGACAAACTGGCggggtgt      >  2:331492/5‑244 (MQ=255)
                                                                                                                                                                                                                         catCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGGACA‑CAATGACTGAAATGAGGGTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGAATTTTGCCAAACTGGCGGATGTAGCg   >  1:217588/3‑250 (MQ=255)
                                                                                                                                                                                                                          atCACGTTCTTTATAAAGCTGTGCGAGGGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAGAAGACGGCTCCAGGTCCACCGGCGGTAATTGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCC‑AGCCTGGGACA‑CCAAGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAAGGCTATTTTTATGACTTTTTCCAAACAGGCGGGTGTAgagg  >  2:445052/2‑247 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                    
ATTGCCAGCAGAGTAAAGACAGCGAGAATAATGCCTGTCACCAGCGGGGGAAAATCAAAAGAAAAACTCAGGGCGCGGGCAACGGCGTTCGCTTGAACTCCGCTGAAAATTATGCCATAGGCGATGAGCAAAAAGACGGCGAACAGAACGCCCATCCAGCGCATCCCCAGCCCGCGCGCCATATACCATGCCGGTCCGCCACGAAACTGCCCATTGACGTCACGTTCTTTATAAAGTTGTGCCAGAGAACATTCGGCAAACGAGGTCGCCATGCCGATAAACGCGGCAACCCACATCCAAAAGACGGCTCCAGGTCCACCGGCGGTAATAGCCAGCGCAACGCCGGCCAGGTTGCCGCTACCCACGCGCGCCGCA‑AGACTGGTACA‑CAATGACTGAAATGAGGTTAAACCGCCTGGCTGTG‑GATGAATGCTATTTTTAAGACTTTTGCCAAACTGGCGGATGTAGCGA  >  NC_000913/7382‑7849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑