Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,238,267	IS1 (+) +9 bp	coding (1015‑1023/1521 nt)	mglA ←	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1978503 =	NA (NA)	65 (1.040)	58/490	0.2	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	2238267 =	0 (0.000)					coding (1023/1521 nt)	mglA	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
*	?	NC_000913	= 1979270	NA (NA)	43 (0.690)	36/490	0.6	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 2238275	0 (0.000)					coding (1015/1521 nt)	mglA	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

CACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGA  >  NC_000913/1978480‑1978751                        |                                                                                                                                                                                                                                                         acaacgacgatatcatcatgctGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATGCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATcct                       <  2:81322/230‑2 (MQ=11)      gactatatcaacatgctGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGCCTTTGTCATGCAGCTCCACCGATTTTGAGCACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCATTTTCAGGTTATGCCGCTCAATTCGCTGCGTCTATCGCTTGCTGATTCCGTTCAGCTTTCCCTTCTGGCGGTATTCCTACAGCGGCCATCCATCCGTCATCCctatac                  >  2:249102/17‑249 (MQ=11)            atcaacatgctGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGt            >  1:62112/11‑251 (MQ=11)             tcaacatgctGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTc           >  2:550499/10‑251 (MQ=11)                    gctGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATACTGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAgggg    >  1:230319/3‑250 (MQ=11)                      tGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTg   >  1:103652/1‑250 (MQ=11)                      agggTAATGACTCAAACTTATTGATAGCGTTTTATATTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCACTTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTACCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATTTCACCACGTCAAAggggg   >  2:442588/2‑248 (MQ=11)                       gggTAATGACTCCAACTTATTGATAGGGTTTTATGTTCAGATAATGTCCGATGACTTTGTCATGCAGCTCCAACGCTTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGAGCCAGGTTATGCCGCTCAATTAGCTGCGTATATCGCTTGCTGATTACGTGCGGCTTTCCCTTCAGGCGGGGTTCGTACAGCGTCCAGAAATCCGTGATCCATATCGCCACGTCAAAGGGTGa  >  1:341482/1‑250 (MQ=11)                        |                                                                                                                                                                                                                                                         CACTCAATTACTTAACATAAATGGGTAATGACTCCAACTTATTGATAGTGTTTTATGTTCAGATAATGCCCGATGACTTTGTCATGCAGCTCCACCGATTTTGAGAACGACAGCGACTTCCGTCCCAGCCGTGCCAGGTGCTGCCTCAGATTCAGGTTATGCCGCTCAATTCGCTGCGTATATCGCTTGCTGATTACGTGCAGCTTTCCCTTCAGGCGGGATTCATACAGCGGCCAGCCATCCGTCATCCATATCACCACGTCAAAGGGTGA  >  NC_000913/1978480‑1978751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.