Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,235,323	IS2 (+) +5 bp	coding (386‑390/720 nt)	fadR →	DNA‑binding transcriptional dual regulator FadR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1235323 =	0 (0.000)	40 (0.640)	40/490	0.5	100%	coding (386/720 nt)	fadR	DNA‑binding transcriptional dual regulator FadR
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region
*	?	NC_000913	= 1235327	0 (0.000)	27 (0.430)	26/490	1.0	100%	coding (390/720 nt)	fadR	DNA‑binding transcriptional dual regulator FadR
	?	NC_000913	= 1469240	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

GAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1235085‑1235327 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTAT  <  NC_000913/1469240‑1469011                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            GAAGTGTTACAGCGTCTGGGACGAAATGGCTGGTTGACCATTCAACATGGCAAGACGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTGTCGTCAGCATCCCTATAAAGCGCAGGAAGTGCTGGGTACCTAGACTGG                                                                                                                                                                                                                                 <  1:477360/251‑1               TCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCC                                                                                                                                                                                                                   >  2:8494/1‑251                   GCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACCTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTAGTCGCACCGCGTGGCGTCAGCTTCCCGATAAAGCGCAGGAAGTGCTTGCGACCTAGACTGGCCCACGGAATCTCCAGAC                                                                                                                                                                                                               <  2:602502/251‑1                     ACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA                                                                                                                                                                                                              >  2:192484/1‑250                        AGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATATTTTCTGGGAAACTTCCGGTTTAAATATCCTTGACACAATGGAGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACATAGACTGGCCCCCTGAATCTCCAGACA‑ACC                                                                                                                                                                                                          >  1:44342/1‑250                            GGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTTCCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATAT                                                                                                                                                                                                     <  2:337657/251‑1                                                 GGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTT‑ATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTT                                                                                                                                                                               <  2:376404/251‑1                                                  GCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACGGGCCCCCTGAATCTCCAGACG‑ACCAATATCACTTAAATAAGTGATAGTCTT                                                                                                                                                                               <  1:262028/251‑1                                                     AGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAAT                                                                                                                                                                            >  1:95971/1‑251                                                                      AATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA                                                                                                                                                            <  1:541506/250‑1                                                                                     gCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTAGTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATAGCACTTAAAGAAGTGATAGTCTTAAGACTAGTTTTTAGAATAGTCATTGGATAACAG                                                                                                                                             <  2:44342/249‑1                                                                                                     TTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAT                                                                                                                                           >  1:244493/1‑236                                                                                                     TTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACGAGTTTTTAGACTAGTCATTGGAGAACAGAT                                                                                                                                           <  2:244493/236‑1                                                                                                      TGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAG                                                                                                                                                           <  1:457450/219‑1                                                                                                      TGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCAAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAG                                                                                                                                                           >  2:457450/1‑219                                                                                                            ACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCGCCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑AC                                                                                                                                                                                                           <  1:406079/165‑1                                                                                                            ACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑AC                                                                                                                                                                                                           >  2:406079/1‑165                                                                                                                 CGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGAGGTCTTAGGGCCGGAGAAACG                                                                                                                <  1:476581/251‑1                                                                                                                   CGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGC                                                                                                               >  1:499800/1‑250                                                                                                                                                       AATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTCAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTC                                                                          >  1:335884/1‑251                                                                                                                                                       AATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTC                                                                          <  2:137233/251‑1                                                                                                                                                               GTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAG                                                                   <  1:8494/250‑1                                                                                                                                                                     GCGTACCACTATTTCCACTATTTTTATTCGCACCGAGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGA                                                             >  1:140756/1‑250                                                                                                                                                                         ACCAATATTTCCACTCTTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAACGAGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACAGACCA‑TATCACTTAACTAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAAGCCCGGAGCACCGCAGACGGCTTACAACACAGGAAAAGCTCGCAATTGTTCAGCAGAGCTTTGAACCGG                                                        >  2:80974/1‑251                                                                                                                                                                                            TTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTTCCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATCTCACTTCAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGT                                     >  1:28272/1‑251                                                                                                                                                                                                       GCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑CCAAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAAC                          >  1:588785/1‑251                                                                                                                                                                                                                               GCGCAGGAAGTGCTGGCTACCTAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTAT  <  1:277895/251‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            GAAGTGTTACAGCGTCTGGCACGAGATGGCTGGTTGACCATTCAACATGGCAAGCCGACGAAGGTGAATAATTTCTGGGAAACTTCCGGTTTAAATATCCTTGAAACACTGGCGCGACTGGATCACGAAAGTGTGCCGCAGCTTATTGATAATTTGCTGTCGGTGCGTACCAATATTTCCACTATTTTTATTCGCACCGCGTTTCGTCAGCATCCCGATAAAGCGCAGGAAGTGCTGGCTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1235085‑1235327 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACA‑ACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTAT  <  NC_000913/1469240‑1469011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.