Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,542,692 +A intergenic (+59/‑423) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,542,6921.A85.3% 86.8 / 11.2 34intergenic (+59/‑423)fimE/fimAregulator for fimA/type 1 fimbriae major subunit
Reads supporting (aligned to +/- strand):  ref base . (2/3);  new base A (11/18);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

CATATGTTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGC‑CAAA‑C‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATT  >  NC_000913/4542442‑4542929
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              
catagtttaaggcaggtgtGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑Caaa                                                                                                                                                                                                                                                 <  2:463934/232‑1 (MQ=255)
        aaGGCATGCTGGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCat                                                                                                                                                                                                                                       <  1:460815/251‑1 (MQ=255)
                  tGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTCTC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAACTAATCTCATAAACGACAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCataaat                                                                                                                                                                                                                              >  2:624223/1‑250 (MQ=255)
                      gTTAGGAAGTGGCGGAGCATGGTGCAGATACTCGTTTAATTCAGGATTATC‑TCGGGCAGCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGTGC‑CAAAAC‑TGTCCATATCATAAATAAGtt                                                                                                                                                                                                                         <  2:481344/251‑1 (MQ=255)
                             aTTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAAAAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTAtt                                                                                                                                                                                                                  >  1:193174/1‑251 (MQ=255)
                                      gCGTGGTGCAGATACTCGTTTAATTCAGGATTATC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCaa                                                                                                                                                                                                         <  1:299611/251‑1 (MQ=255)
                                                     tCGTTTGATTCAGGAAT‑TCATCGGGCTTCGAAATATTCGCCATACGGTGCGTTATACGGCCAGTAATGCTGCTCGTTTTCCCGGAATCTGGGAAAGAAATAGTCCAATAAACGAGAAGTTAAAAAGAGACGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGAATTTTTTCTCAAGCATAAAAATATTa                                                                                                                                                                                           >  2:408391/1‑250 (MQ=255)
                                                     tCGTTTAATTCAGGATTATC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATACGTTACGTATTTTTTCTCAAGCATAAAAATATTa                                                                                                                                                                                           >  1:124280/1‑250 (MQ=255)
                                                           aaTTCAGGATTATC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTTCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACg                                                                                                                                                                                    <  1:308368/251‑1 (MQ=255)
                                                             ttCAGGATCATC‑CCGGGCATCGAAATATTCGCCAGACTGTGCGTTAAACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGa                                                                                                                                                                                   <  2:464366/250‑1 (MQ=255)
                                                                   aTTATC‑TCGGGCATCGAAATAGTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAg                                                                                                                                                                            <  2:263114/251‑1 (MQ=255)
                                                                       tc‑tcGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATc                                                                                                                                                                        >  1:459102/1‑251 (MQ=255)
                                                                               cagcaaatgttaccccATACTGTGGGTGATTCCGCCAGTAATGCTGCTCGTGTTGCCGGAGTATGGGAAAGAAATAATCTCAAAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGCCAAT‑GTGGC‑CAAA‑CCTTTCCAGATCATAAATAATTGACGTATTTTGTCTCAAGCATAAAAATAT‑GAAAAACTACAAAAATGATCTAACTGtt                                                                                                                                                                <  2:398086/237‑1 (MQ=255)
                                                                                                    gtgCGTTATACCGCCAGTAATGCTGCTCGGTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGCTAAAA‑C‑TCTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCt                                                                                                                                             <  1:560299/250‑1 (MQ=255)
                                                                                                    gtgCGTTAAACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTc                                                                                                                                              <  2:118981/249‑1 (MQ=255)
                                                                                                     tgCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAACAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTCTTTCTAt                                                                                                                                           >  1:80475/1‑251 (MQ=255)
                                                                                                                              tcgatttGCCGGATTATGGGACAGACATAATCTCATAAAAGAAAAATTATCAAGAGAAGAGGTTTGATTTAACTTATTGCTAATAAAGTTAAAAACACACATAAAAACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCAAAAATCAGTTACGTATTTTTTCTCAAGCCTAAAAATATTAACAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTATATTGTAAATTATATTCCCATCACCTcc                                                                                                                  >  2:275971/5‑251 (MQ=255)
                                                                                                                               cGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTCACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTc                                                                                                                   <  2:426879/249‑1 (MQ=255)
                                                                                                                                           ttATGGGAAAGAAATAATCTCATAAACGCAAAATTAAAAAGAGAAGAGGATTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATAATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTaa                                                                                                       >  2:267063/1‑249 (MQ=255)
                                                                                                                                                                    aCGAAAAATTAAAAAGAGAAGATGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAAGTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCAt                                                                            <  1:586479/251‑1 (MQ=255)
                                                                                                                                                                           aTTAAAAAGAGAAGAAGTTAGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATCAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATAATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATAACCTCCGCTATATGTAAAGCTAACGTTTCTTTGGCTCAACGCATCTTACTc                                                                     >  2:101131/1‑251 (MQ=255)
                                                                                                                                                                              aaaaaGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATGAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCAtt                                                                  <  1:119901/251‑1 (MQ=255)
                                                                                                                                                                                    agaagagGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctc                                                            <  1:51352/251‑1 (MQ=255)
                                                                                                                                                                                      aagagGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATGAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTAAATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctctc                                                          <  2:516163/251‑1 (MQ=255)
                                                                                                                                                                                            tttGATTTAACTTATTGATAATCCAGTTAAAATAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TATCCATATAATAAATAAGTTAAGTATTTTTTCTCAAGCATAACAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGATAACGTTTCTGTGTCTCGACGCATCTTCCTCATTCTTCTCTCCcaaac                                                    >  2:72653/1‑250 (MQ=255)
                                                                                                                                                                                                      cTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATAGGGCC‑AAAA‑C‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTAAAGCATAAAACTATTAAAAAAAGACAAAAAGCATCTAACTGTTTGCTATTTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTATTTGGCTCGACGCATCTTCCTCCTTCTTCTCTCCAAAAACCACCTCAtt                                          >  1:584533/1‑250 (MQ=255)
                                                                                                                                                                                                        tagtGATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAACCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCTCCTCATg                                          <  1:76719/246‑1 (MQ=255)
                                                                                                                                                                                                        tATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATGGGGAC‑AAAA‑C‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTGCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAAACACCTCATg                                          <  1:10493/249‑1 (MQ=255)
                                                                                                                                                                                                            gATAATAAAGTTAAAAAAACAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAata                                     <  1:589730/250‑1 (MQ=255)
                                                                                                                                                                                                                          aaaaaCAAATAAAAACAAGACAATGGGGAC‑AAAA‑C‑TGTCCATATCATAAATAAGTTACGTCTTTTTTCTCAAGCATAAAAATATTAACAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTataaat                      >  2:377766/1‑251 (MQ=255)
                                                                                                                                                                                                                               cAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATGCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGa                 <  1:323936/251‑1 (MQ=255)
                                                                                                                                                                                                                               cAAATAAATACAAGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCAGAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGGTATGTAAATTATTTCGATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGa                 <  1:580619/251‑1 (MQ=255)
                                                                                                                                                                                                                                           aGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAat     <  2:193174/251‑1 (MQ=255)
                                                                                                                                                                                                                                           aGACAAT‑GGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAat     <  1:33837/251‑1 (MQ=255)
                                                                                                                                                                                                                                                aatGGGGC‑CAAAAC‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATAtt  >  2:121206/3‑250 (MQ=255)
                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                              
CATATGTTAAGGCATGCTTGCGGTTATGAATTGGCGGAGCGTGGTGCAGATACTCGTTTAATTCAGGATTATC‑TCGGGCATCGAAATATTCGCCATACTGTGCGTTATACCGCCAGTAATGCTGCTCGTTTTGCCGGATTATGGGAAAGAAATAATCTCATAAACGAAAAATTAAAAAGAGAAGAGGTTTGATTTAACTTATTGATAATAAAGTTAAAAAAACAAATAAATACAAGACAATTGGGGC‑CAAA‑C‑TGTCCATATCATAAATAAGTTACGTATTTTTTCTCAAGCATAAAAATATTAAAAAACGACAAAAAGCATCTAACTGTTTGATATGTAAATTATTTCTATTGTAAATTAATTTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATT  >  NC_000913/4542442‑4542929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑