Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,601	(T)5→4	coding (1251/1401 nt)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,601	0	T	.	100.0%	152.0 / NA	33	coding (1251/1401 nt)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (18/15);  total (18/15)

ACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAG  >  NC_000913/3025358‑3025846                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       acggtaggTCGGGCGAACCAGCGCGGTAATGCTTGTTATCCACGGCTTATTTCCGATGATGGGCGGCTTCTGCACGACCATTCCCTCGTCAGTTCTGGGCGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCTCATCACCATCACCAACTGTTTAAAGCGCCGTGAAACCCTTATTGTCGCCACTTCTTTAAGTTTAGGGCTTGGCGTCTCCTCCGATCCCGAAATTTT‑AAAATAtt                                                                                                                                                                                                                                                <  2:463105/244‑1 (MQ=255) aCGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATAtt                                                                                                                                                                                                                                                <  1:599388/251‑1 (MQ=255)      aTGTCGGGCGAACCATCGAGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGcc                                                                                                                                                                                                                                             >  2:400385/1‑249 (MQ=255)             gCGAACCATCGGGTTAAGGCTGGTTATCCTCGGCTTATTGCCGATGATTGGCGGCTTCTGCACTATCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTACCATGAGTGCCATCTCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTATCCTACGATCCCGAAATTAT‑AAAATATTGCTAGCCTCTAt                                                                                                                                                                                                                                    <  2:572499/251‑1 (MQ=255)                  ccATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTTCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCTAGCCTCTATTTATg                                                                                                                                                                                                                               <  2:573825/251‑1 (MQ=255)                         ggTAATGCTGGTTATCCTCGGCTTATTTGCGATGATTGGCGGCTTCTTCATGGACATTCACTCGGCAGGTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAAGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTGTAGGTCTTGGCGTCTCCTAAGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGt                                                                                                                                                                                                                        <  2:390531/251‑1 (MQ=255)                                   gTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCt                                                                                                                                                                                                              <  1:400385/251‑1 (MQ=255)                                      aTCCTCGGCTTATTTCCGATGAAGGGGGGCTTCTTAACGACCATTCACTCGGCAGTTCACGGAGGCGCAATGACTTTGATGTTTTCCATGATTGCCATCGCAGGGAATCGCATCCTCATCACCAACGGTTTCAAGCGCCGAGCAACCTTTATTGTCGCCTCTTCTTTAGGTTTAGGGCTTGGCGTCTCCTCCGATCCCGCAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTAtt                                                                                                                                                                                                           >  2:61734/1‑251 (MQ=255)                                         cTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCCATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGACAACCCTATTtgt                                                                                                                                                                                                        >  2:564130/1‑251 (MQ=255)                                                                cggcTTCTTCACTACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAATCACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTAGTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGAtttt                                                                                                                                                                                <  1:139484/251‑1 (MQ=255)                                                                     tcttcACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAGTATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTttatt                                                                                                                                                                             <  2:174213/249‑1 (MQ=255)                                                                     tcttcACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAAATGCGATTttattt                                                                                                                                                                            >  2:50296/1‑249 (MQ=255)                                                                       ttcACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAat                                                                                                                                                                         >  1:396881/1‑251 (MQ=255)                                                                         cACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGCTTTTATTTAatat                                                                                                                                                                       >  1:586844/1‑251 (MQ=255)                                                                                        ggCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AACATATTGCCAGCCTCTATTTCTGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTTACTGCGATTTTATTAAATCTTTTCCTCCCtggtgg                                                                                                                                                        >  1:338037/1‑251 (MQ=255)                                                                                        ggCAGTTCGGGGAGGCGCAATGACGTTGATGTTTGCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAACATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTTGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCtggtgg                                                                                                                                                        <  1:464974/251‑1 (MQ=255)                                                                                         gCAGGTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGc                                                                                                                                                       <  2:345928/251‑1 (MQ=255)                                                                                                  ggAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑GTAACTGCGATTTTATTAAATATTTTCCTCCCTGGGGGCTAcccac                                                                                                                                                >  1:65276/1‑246 (MQ=255)                                                                                                   gAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGGTTTTTTTAAATATTATCCTCCCTGGTGGCTACCGACAgg                                                                                                                                             >  1:85950/1‑251 (MQ=255)                                                                                                     ggCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGGGTTAA‑TTCGATTTTTTTAAAATTTATCCTCCCtggtgggtaacgccaggg                                                                                                                                            >  2:516768/1‑238 (MQ=255)                                                                                                       cgcAATGACGTTGATGTTTTCCATGATTGCCATAGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTCTTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTTAATGCGATTTTTTTTAATATTTTTCTCCCTTGTGGCTAcctgcatgcaa                                                                                                                                          >  1:493321/1‑241 (MQ=255)                                                                                                           aTGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGACACACTTATTGACGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTTAATGCGCTTTTATTTAATATTCTCTTCCCTGGTGGCTACCGACAGGTAAAAGtt                                                                                                                                     >  1:258413/1‑251 (MQ=255)                                                                                                                     tGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGCAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑GTAACTGCGATTTTATTAAATATTATCCTCCCTGGAGGGTACCGACAGGAAAATGTTCtcgccgggg                                                                                                                           >  1:50904/1‑243 (MQ=255)                                                                                                                                            gggATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGAt                                                                                                    <  1:189409/251‑1 (MQ=255)                                                                                                                                              gATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTa                                                                                                  <  2:189257/251‑1 (MQ=255)                                                                                                                                                                 aaCGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGGTTTTTTTAAATATTATCCTCCCTGGTGGCTAACGACAGGAAAAAGTTCTGCCTGGTATTACCTCACCGGAAGAGGTGGATTAACAGTAAAGGGGTCaaatt                                                                               >  2:344499/1‑248 (MQ=255)                                                                                                                                                                 aaCGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAatga                                                                               <  1:71132/251‑1 (MQ=255)                                                                                                                                                                                                            taggtttaggGCTTGGCGTCTCCTACGTTCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTTACCGCGATTTTTTTAAATATTATTCACCCTTGTGGCTACCGACAGGAAAACGGTCTGCCTGGTATTACCTCCACGGGAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGGGAAAACCCCTTAAAAGCTGTACGAGGAAGTTTTAt                                    >  1:179052/1‑251 (MQ=255)                                                                                                                                                                                                                tttaggGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTc                                                                                                                    <  2:153748/167‑1 (MQ=255)                                                                                                                                                                                                                tttaggGCTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCCGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTc                                                                                                                    >  1:153748/1‑167 (MQ=255)                                                                                                                                                                                                                       cTTGGCGTCTCCTACGATCCCGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGCTTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAAAGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGGTTAACAGTAAAGGAGTCAATGATGTCAGGGGAACACACGTTAAAAGCGGTACGAGGCAGGTTTATTGATGTCAccc                         >  1:174788/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTTACTGCGGTTTTTTTAAATATTATCCTCCCTGGTGGCTACCGCCAGGAAAACGTTCTGc                                                                                                                                 >  1:209251/1‑128 (MQ=255)                                                                                                                                                                                                                                          ccGAAATTTT‑AAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGc                                                                                                                                 <  2:209251/128‑1 (MQ=255)                                                                                                                                                                                                                                                attttAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTTACTTCGTTTTTTTTAAATATTATCCTCCCTGGTGGCTACCGCCAGGGAAACGTTCTGCCTGGTATTACCTCAGCGGGAGAGACGGGTTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTTTTGATGTCACCCGTACGATCGATAACCCGGAagcg  >  2:208711/2‑248 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       ACGTTATGTCGGGCGAACCATCGCGGTAATGCTGGTTATCCTCGGCTTATTTCCGATGATTGGCGGCTTCTTCACGACCATTCCCTCGGCAGTTCTGGGAGGCGCAATGACGTTGATGTTTTCCATGATTGCCATCGCAGGGATTCGCATCATCATCACCAACGGTTTAAAGCGCCGTGAAACACTTATTGTCGCCACTTCTTTAGGTTTAGGGCTTGGCGTCTCCTACGATCCCGAAATTTTTAAAATATTGCCAGCCTCTATTTATGTATTAGTTGAAAACCCTATTTGTGCTGGCGGG‑TTAACTGCGATTTTATTAAATATTATCCTCCCTGGTGGCTACCGACAGGAAAACGTTCTGCCTGGTATTACCTCAGCGGAAGAGATGGATTAACAGTAAAGGAGTCAATGATGTCAGGAGAACACACGTTAAAAGCGGTACGAGGCAGTTTTATTGATGTCACCCGTACGATCGATAACCCGGAAGAG  >  NC_000913/3025358‑3025846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑