Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3630C.88.2% 112.2 / 7.7 34intergenic (‑490/+919)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base . (18/12);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009132,173,3640C.88.2% 110.5 / 9.5 34intergenic (‑491/+918)gatD/gatBgalactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base . (18/12);  total (20/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

CCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGC  >  NC_000913/2173130‑2173614
                                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                                          
ccgccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCagcagc                                                                                                                                                                                                                                          >  1:509341/1‑251 (MQ=255)
 cgccagttgggtgggccccacactggTTTGCGTCGCGTTCCACAGGGTGATGCTCATACTGATCACACCCGAGCTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGCAGCCGATGGCGGCAAGATCGCCAAACGGCAGCCCCTGCTTACCCGGCACCTAAACAGCACTTACAATGG‑TGAGTGGCATAACAATAAGGCTTGCCGA‑ACC‑CACCGCCGTATGTCCCagcagc                                                                                                                                                                                                                                          >  2:84254/26‑250 (MQ=255)
 cgccAGTTGGGTGTGCAACCCAAGAGTTTGCGCCGCGATCCACAGGGGGATGCTCATAATGATGACACCCGAGATAAAGGTGCGGAACAGATTTCCACGATGCCCGGCGACCGCCATCGCAGCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCGGCACCTGATTACCCGGCACACAAACAGCAATAAAAATGG‑TGAGTGGGATAAAAATCAGGTTTGCCGATAC‑‑CTCCGCCGTATGTCCCAgcagca                                                                                                                                                                                                                                         <  1:457977/251‑1 (MQ=255)
  gccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAgcagca                                                                                                                                                                                                                                         >  1:353262/1‑250 (MQ=255)
        tGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCgg                                                                                                                                                                                                                                  >  1:426222/1‑251 (MQ=255)
         gggtggccaacccaataggtgccttCGCAATCCAAAGGGTGATGCTCAAAATGAGGCCACCCGATATTAAGGTTCGGAACAGATTTCCACAATACGCGGCGACCGCCATCGCCACAAAGAAGCGGTTGGTGGCAAGATCGCCAAACGGCCGCACCTAATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGa                                                                                                                                                                                                                                 <  2:497727/227‑1 (MQ=255)
                    ccAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAt                                                                                                                                                                                                                      >  1:30975/1‑251 (MQ=255)
                                  cgcgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGAATCAAGGCCAATCAGGAACTCCTGAc                                                                                                                                                                                                        >  2:127248/1‑251 (MQ=255)
                                     gATCCACAGGGTGATGCTCATAAGGATGACACCCGAGATTAAGGGGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGAAAGACCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTGGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc                                                                                                                                                                                                     <  2:87383/251‑1 (MQ=255)
                                           caGGGTGATGCTCATAATGATGACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACt                                                                                                                                                                                               <  1:556531/251‑1 (MQ=255)
                                            ggggTGATGCTCATAATGAGGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCTATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                                                                                                                                              <  1:58751/250‑1 (MQ=255)
                                                     cTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑GGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTa                                                                                                                                                                                      >  2:451679/1‑250 (MQ=255)
                                                           atgatgACCCCCGAGATTAAGGTGCGGAACAGATTTCCACGAGGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt                                                                                                                                                                               <  1:35227/251‑1 (MQ=255)
                                                            gaatgaCCCCCGAGATTAAGGTGCGGAACAGATTCCCACGATGCACGGCGACCGCCATCGCCACAAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAAGGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACGCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                                                                                                                                              <  1:509308/249‑1 (MQ=255)
                                                               tgaCACCCGAGCTTAAGGTGCGGAACCGATTTCCACGTTGCAAGGCGACCGGCATCGCCACGAAGACGCCGATGGTGGCCAGATCGCCAAACGACAGCCCATGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAGAATCAGGCTTGCAGATAC‑‑CCCCGCCGTATGGCACAGCAGTAACTCCGGCTCCAGGCCAATCAGGAACTCCGAACCGCCGAACTTCGCCTGTAAACTACTACg                                                                                                                                                                            >  2:359763/1‑250 (MQ=255)
                                                                    ccggagaTGAAGGTGCGGAAAAGATTTCCACGATGCACGGGGGCCGCCATCGCCACGAAGAAGCGGATGGTGGCAAGACCGCAAAAGGGCAGCACCTGATTACCCGGCACACAAACAGAAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcttg                                                                                                                                                                      <  1:120319/248‑4 (MQ=255)
                                                                             aaGGTGCGGAACAGATTTCCACGATGCGCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAACATGG‑TGAGTGGGATAAAAATCGGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGAAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTCGCGAt                                                                                                                                                             >  2:330835/1‑251 (MQ=255)
                                                                                gTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATggg                                                                                                                                                          >  1:384637/1‑251 (MQ=255)
                                                                                   cGGAACAAATTTCCCCGAGCCACGGCGACCGCCTTCCCCCCGAAGAAGCCGTGGGGGGCAAGATCGCCAAACGTCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAACCGGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGATCTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGt                                                                                                                                                       <  1:359763/251‑1 (MQ=255)
                                                                                         aGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAAcc                                                                                                                                                 >  1:234841/1‑251 (MQ=255)
                                                                                                                             aagaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAGCACCCGTGGCATTagcag                                                                                                             <  1:508706/251‑1 (MQ=255)
                                                                                                                               gaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGAACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCAGGATGGGTTTAATCACCCGTGGCATTagcagc                                                                                                            >  2:22263/1‑250 (MQ=255)
                                                                                                                                  gCCGATGGTGGAAAGATCGCCAAACGGCAGCATCTGCTTACCCGGCACACACACCGCAATTAAACTGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGCCTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGCCATTAGCAGCATc                                                                                                         >  2:74074/1‑250 (MQ=255)
                                                                                                                                   ccGAGGGTGGCAAGAACGCAAAACGGCAGCACCTGATTACCCGGCACAAAAACAGCAATTAAAATGG‑GGAGGGGGATAAAAATCAGGCTTGCAGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCAc                                                                                                       <  2:426222/251‑1 (MQ=255)
                                                                                                                                               aGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGtttt                                                                                           >  1:459074/1‑251 (MQ=255)
                                                                                                                                                  tCGCCAAACGGCAGACCCTGATTACCCGGCACACAAACAGCACTTAAAATGG‑TGAGTGGGATAAAAATCATGCTTGCCGATACCACC‑‑GCCGAATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCAGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGAATTAGCAGCATCACTGCTGCAGTTTTTAc                                                                                        >  2:85672/1‑251 (MQ=255)
                                                                                                                                                        aaCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                    >  1:515405/1‑249 (MQ=255)
                                                                                                                                                           ggCAGGACCTGATTACAAGGAACAAAAACAGCAATTAAAATGG‑CGAGCGGGATAAAACTCAGGCTTGCCGATACCACC‑‑GACGTATGTCCAAGCAGCAACGCCGGATCAAGGCCCATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTACTCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTg                                                                               >  2:326371/1‑251 (MQ=255)
                                                                                                                                                                 ccctaatcccccGCCACAAAAACAGCAAT‑AAAATGGGTGATGGGGAAAAAAATGAGGCTGGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAACCAGGAACTCCTGCCCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATac                                                                         <  2:353262/242‑1 (MQ=255)
                                                                                                                                                                         ccccGTCACCCAAACAGCAATAAAAAGGG‑TGAGTGGGATAAAAACCAGCCTTGCCGAAAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGa                                                                 <  1:456117/250‑1 (MQ=255)
                                                                                                                                                                          cccGGCACACAAACAGAAATTAAGATGT‑AGCGTGGGATAAAAATCAGGCTTGCCGATACAACC‑‑GCCGTATGTACCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACAGCCGCAGTTTTTACAGCCAGCTGCAATACACCttttac                                                                >  2:541762/1‑248 (MQ=255)
                                                                                                                                                                                             ttAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAggctg                                             >  2:538388/1‑248 (MQ=255)
                                                                                                                                                                                                         aGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc                                    >  1:576672/1‑249 (MQ=255)
                                                                                                                                                                                                         aGTGGGATAAAAACCAGCCTTCCCAATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAg                                  <  1:127248/251‑1 (MQ=255)
                                                                                                                                                                                                                                           cgccgTCTGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGc  >  2:559170/1‑251 (MQ=255)
                                                                                                                                                                                                                                           cgccgTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCCCAAAACCCACGGTGACAGGCTCGc  >  2:540796/1‑251 (MQ=255)
                                                                                                                                                                                                                                          ||                                                                                                                                                                                                                                                          
CCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGG‑TGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGC  >  NC_000913/2173130‑2173614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑