Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,985,729	C→T	L93L (CTG→CTA)	aslA ←	putative sulfatase AslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,985,729	0	C	T	100.0%	212.2 / NA	62	L93L (CTG→CTA)	aslA	putative sulfatase AslA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/35);  total (27/35)

GCAGCGTGGTTAACCCTTGCAGCCCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCCAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGACGGGGTATCTGCTGCGCTGGCCATGAGAGGAAGAGCGGCGGCGACAGCAA  >  NC_000913/3985491‑3985973                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     gCAGCGTGGTTAACCCTTGCAGCCCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCCCATCGTCTAGCAAGAAaac                                                                                                                                                                                                                                           >  2:210081/1‑250 (MQ=255)  cAGCGTGGTTAACCCTTGCAGCCCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGGCTAGCAAGAAaacaa                                                                                                                                                                                                                                         <  1:473999/251‑1 (MQ=255)    gCGTGGTTAACCCTTGCAGCCCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAAcc                                                                                                                                                                                                                                       >  2:155082/1‑251 (MQ=255)           aaaCCCTTCAAGCCCGCCGGGTTGCCCGACCATGGGCGGCAGCAGAAGGCCTTGGTGA‑GTGGAGTAGTGTACGGTGAGAATGGTGGGGCGGGTTGGGGAAGAGCTTGGTTGGGAATACGCCGAATGTAAAATCAGCCCCTGGCTGGGAACGGCGGTGATATCTGGTG‑GAGGGTTACCCCCCGCCTCGCCGCCACCGTTAAAACCGACGTCCATCCAGACCACAACGTTTAACAAGAAAACAACCACATTc                                                                                                                                                                                                                                 <  2:247434/249‑1 (MQ=255)            aaCCCTTGCAGCCCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCg                                                                                                                                                                                                                                <  2:15225/250‑1 (MQ=255)            aaCCCTTGCAGCCCGCCCGGTGGCCCGTACATGGGCGGCATCAGAATGCCGTGGTGG‑AGGGAGTATTTTCCGGTGAGAAGGGTGGCGCGGGTGGGGGAAGAGCTTGGTTGAGAATACGCCGAATTTAAAATCAGCCCCGGGCTGGCAACGGCGTCGATATCGGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCg                                                                                                                                                                                                                                <  2:544139/250‑1 (MQ=255)             aCCCTTGCAGCCCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGt                                                                                                                                                                                                                              >  1:104454/1‑251 (MQ=255)                cTTGCAGACCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGCCTTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGGGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGAGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGCCGTCCATCCAGCCCACATCGTCTAGAAAGACAACAACCACATTCGGTTTc                                                                                                                                                                                                                           >  2:66738/1‑251 (MQ=255)                  tGCAGCCCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTTAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTc                                                                                                                                                                                                                           <  1:66738/249‑1 (MQ=255)                       cccgcccgGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTGGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGTTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATGCGGTTTCGTACCgg                                                                                                                                                                                                                    <  2:55020/251‑1 (MQ=255)                              ggtggCCCGTACATTGGGGGCATCAGAAGGCCGTGGGGG‑ATGGAGTATTGTCCGGTGGGAATGGTGGAGCGTGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCCCCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGttttttt                                                                                                                                                                                                             <  2:316849/247‑1 (MQ=255)                                        aCATTGGCGGCAGCAGATGGCCGTGGTGG‑AGGGGGTTGTGTCTGGTGAGAATGGTGGCGCGGGTGGGGGAAGAGCTTGGTTGAGAATACGCCAAAGTTAAAATCACCCCCTGTCTGGCAACGGCGTGGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTc                                                                                                                                                                                                    <  2:99555/250‑1 (MQ=255)                                             gggggtataggAATGCC‑TGGTGGGATGGGGTATGGCCCGGGGTGAATGGGGGCCCGGGTGGGGGAAGCGCTTGGTTGAAAATACGCCGAGTTTAAAACCACCCCCGGGCTGGCAACTCCGTCGAAATC‑GGTGGTAGTTTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACGTCGTCTAGCAAGAAAACAACCGCATTCGGTTGCTTACCGGTTTTGTTCTCAAGTTATGCCAg                                                                                                                                                                                              <  2:252825/241‑1 (MQ=255)                                                 gcATCAGAATGCCTTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TCGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTc                                                                                                                                                                                          >  2:107231/1‑251 (MQ=255)                                                 gcATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGGGGCGCGGGTTGGGGAGGGACTTGGTTGAGAATACGCCGACGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTCCCGGTTTTTTTCTCCAGTTCAGCCAGCTTc                                                                                                                                                                                          >  2:329136/1‑251 (MQ=255)                                                         aTGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCGGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGt                                                                                                                                                                                  <  1:556547/251‑1 (MQ=255)                                                          tGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAACTCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGtt                                                                                                                                                                                 >  2:550822/1‑251 (MQ=255)                                                              gtggtgG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCtt                                                                                                                                                                             >  1:264167/1‑251 (MQ=255)                                                                             aTTGTCCGGGGAGAATGGTGGTGTGGGTGGGGGAAGAGCTGGGGTGAGAATACGCCGAAGTTAAAATCAGCCCCTTGCTGGCAACGGCGTAGATAGCTGTTG‑TAGGGTTTCCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCTCACATCGTCTAGCAAGAAAACAACCACAGTCGGTTTCTTACCGGTTTTTTTCGCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGa                                                                                                                                                               <  1:364371/251‑1 (MQ=255)                                                                               tGTCCGGTGAGAAGGGTGGGGCGGGTTGGGGAAGAGTTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCCCCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGTTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGAt                                                                                                                                                              <  1:478750/250‑1 (MQ=255)                                                                                 tCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGAtgct                                                                                                                                                           >  1:455045/1‑251 (MQ=255)                                                                                    ggTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGAtgctgc                                                                                                                                                         >  1:487521/1‑250 (MQ=255)                                                                                      ggagaAGGGTGGCGCTGGTTGGGGAAGAGCTTGTTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGAGATCGGGTG‑TAGGGTTCCCCACCGCCACTCCGCCAGCGTTAAAACCGACGTCCATCCAGCCCACTTCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTGTTCCCAAGTTCTGGCAGCTTCTGCTGGGTTTCTTGATCCTGCGCCTGATGCTGCAtg                                                                                                                                                      <  2:236226/250‑2 (MQ=255)                                                                                             ggtggCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTAAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGc                                                                                                                                                >  1:431340/1‑250 (MQ=255)                                                                                                    cgGGGTGGGGAAGAGCTGGGTTGAGAAGACGCCGAAGGTAAAATCAGCCCCTAGCTGGCAACGGCGTCGATATCTTGGG‑TGGGGTTACCCACCGCCTCGCCGCCACCGTTAAAACCGACGTCCATCCAGTTCACATCGTTTAGCAAGAAAACAACCACATTCGTTGTCTTACTGGTTTTTTTCGCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATAtt                                                                                                                                        <  1:375575/251‑1 (MQ=255)                                                                                                     gggTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTg                                                                                                                                       >  1:223142/1‑251 (MQ=255)                                                                                                     gggTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTg                                                                                                                                       >  1:564876/1‑251 (MQ=255)                                                                                                       gTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTc                                                                                                                                     >  1:57888/1‑251 (MQ=255)                                                                                                            ggAAGAGCTTGGCTGAGACTACCCCGAAGTTAAACTCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGCCGTCCATCCAGCCCACATCGTCTAGCAAGACAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCa                                                                                                                                 >  2:109622/1‑250 (MQ=255)                                                                                                             gAAGAGCTTGGTTGAGAATACGCCGAAGGTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCTACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTTCCGGTTTTTTTCTCAAGTTCTGCTAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAAt                                                                                                                               <  1:511404/251‑1 (MQ=255)                                                                                                                        ttGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCg                                                                                                                    >  1:275271/1‑251 (MQ=255)                                                                                                                         tGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCgg                                                                                                                   >  2:142326/1‑251 (MQ=255)                                                                                                                          gagaATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTCCCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCgg                                                                                                                   <  1:549591/250‑1 (MQ=255)                                                                                                                             aaTACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGcc                                                                                                                     <  1:179781/245‑1 (MQ=255)                                                                                                                             aaTACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGcc                                                                                                                     >  2:179781/1‑245 (MQ=255)                                                                                                                                aCGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAAcc                                                                                                            <  1:431266/251‑1 (MQ=255)                                                                                                                                  gCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCGGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTGCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAg                                                                                                          <  2:223142/251‑1 (MQ=255)                                                                                                                                     gatgTTAAAAGCTGCCCCTGGCTGGCAACGGCGCCTATACCTGGTG‑TAGGGTTACCACCCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCTGTTTAACCAGATa                                                                                                       <  1:112509/248‑1 (MQ=255)                                                                                                                                     gaagggaaaaGCAGCCTCGGGCTGGCAACGTCGGCGATATGGGGTG‑TAGTTTGACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCGCACATCGTCTAGCAAGAAAACAGCCACAGTCGGTTTCTTACCTGTTTGTTTCTCATGTTCTGGCAGCGTCTGCTGGGTTTTTTGATCCTGCGGCGGATGCTGCATTGCTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATa                                                                                                       <  1:534832/245‑1 (MQ=255)                                                                                                                                     gAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTGAAcc                                                                                                            >  2:16985/1‑246 (MQ=255)                                                                                                                                             aaTCCGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTCCCCCCCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTg                                                                                               <  2:178275/251‑1 (MQ=255)                                                                                                                                             aaTCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTg                                                                                               <  1:155082/251‑1 (MQ=255)                                                                                                                                                cAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGCCGTCCATCCAGCCCACATCGTCTAGCAAGAAAACACCCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTgggg                                                                                            >  1:232333/1‑250 (MQ=255)                                                                                                                                                 aGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGt                                                                                                                            >  1:152689/1‑218 (MQ=255)                                                                                                                                                 aGCCCCGGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGt                                                                                                                            <  2:152689/218‑1 (MQ=255)                                                                                                                                                   ccccTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTCAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGAt                                                                                         <  1:376867/251‑1 (MQ=255)                                                                                                                                                     ccTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCg                                                                                       >  1:551247/1‑251 (MQ=255)                                                                                                                                                                    cGTCGATATCTGGTG‑TAGGGTTCCCCTCCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCt                                                                        <  2:148997/251‑1 (MQ=255)                                                                                                                                                                    cGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCATGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCt                                                                        >  1:379078/1‑251 (MQ=255)                                                                                                                                                                     gtcaatatCTGGGG‑TAGGGTTCCCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCTGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCtt                                                                       <  2:363988/247‑1 (MQ=255)                                                                                                                                                                        gATATCTGGTG‑TAGGGTTAACCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCACGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTtgc                                                                    >  2:203811/1‑251 (MQ=255)                                                                                                                                                                                      ggggtcccccccGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTGTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGGCGGCAATAGTGGTCGCCGGTTTACCCAGATACTGGTTTGGGTGATCGTAGCCGGCAAAGCCTTGGCGTGCGGTGGCAGt                                                       <  2:551247/241‑1 (MQ=255)                                                                                                                                                                                          tACCCACCGCCACGCCGTCTCCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACAGTCTGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTTTTTATCCTGCGCCGTATGCTGCATTACTGGCATCATATTGTCTGCAATAGTGGTCGCCGGTTTATCCAGATACTGGTTTGGGTGATCGGATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGa                                                    <  2:203339/250‑1 (MQ=255)                                                                                                                                                                                                   ccACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGACGGGGTATct                                          <  1:109622/251‑1 (MQ=255)                                                                                                                                                                                                   ccACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGACGGGGTATct                                          >  1:460479/1‑251 (MQ=255)                                                                                                                                                                                                   ccACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGCTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGTCAGTTGACGGGGTATct                                          <  1:437821/251‑1 (MQ=255)                                                                                                                                                                                                        ccgccACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGACGGGGTATCtgctgc                                     >  1:239928/1‑251 (MQ=255)                                                                                                                                                                                                               cGTTAAAACCGACGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGACGGGGTATCTGCTGCGCTGGcc                              <  1:142326/251‑1 (MQ=255)                                                                                                                                                                                                                          aCGTCCATCCAGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGACGGGGTATCTGCTGCGCTGGCCATGAGAGGAag                   <  1:157481/251‑1 (MQ=255)                                                                                                                                                                                                                                 tccaGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATc                                                                                        >  1:400976/1‑175 (MQ=255)                                                                                                                                                                                                                                 tccaGCCCACATCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTGTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATc                                                                                        <  2:400976/175‑1 (MQ=255)                                                                                                                                                                                                                                           aTCGTCTAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGACGGGGTATCTGCTGCGCTGGCCATGAGAGGAAGAGCGGCGGCGACAGcaa  <  1:215551/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                     GCAGCGTGGTTAACCCTTGCAGCCCGCCCGGTTGCCCGTACATTGGCGGCATCAGAATGCCGTGGTGG‑ATGGAGTATTGTCCGGTGAGAATGGTGGCGCGGGTTGGGGAAGAGCTTGGTTGAGAATACGCCGAAGTTAAAATCAGCCCCTGGCTGGCAACGGCGTCGATATCTGGTG‑TAGGGTTACCCACCGCCACGCCGCCACCGTTAAAACCGACGTCCATCCAGCCCACATCGTCCAGCAAGAAAACAACCACATTCGGTTTCTTACCGGTTTTTTTCTCAAGTTCTGCCAGCTTCTGCTGGGTTTCTTTATCCTGCGCCGGATGCTGCATTACTGGCATCATATTGTCGGCAATAGTGGTCGCCGGTTTAACCAGATACTGGTTTGGGTGATCGTATCCGGCAAAGCCTTTGCGTGCGGTGGCAGTTGACGGGGTATCTGCTGCGCTGGCCATGAGAGGAAGAGCGGCGGCGACAGCAA  >  NC_000913/3985491‑3985973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑