| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,725,176 | T→G | E48A (GAG→GCG) | glyQ ← | glycine‑‑tRNA ligase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,725,176 | 0 | T | G | 98.6% | 208.7 / NA | 69 | E48A (GAG→GCG) | glyQ | glycine‑‑tRNA ligase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base G (28/40); minor base A (1/0); total (29/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATG > NC_000913/3724932‑3725422 | ccAGGCACCCAGCGGCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGGCGTGAATAGGCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCa < 2:279806/251‑1 (MQ=255) aGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTTTATACTGTTTGGGTTTTCGCCGTAGCT‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGa < 1:238038/251‑1 (MQ=255) tttCCCAGTGATCTTCCACGAAACGGAGGTCGTGAATAGCCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAACCGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTgaga < 1:221220/250‑1 (MQ=255) ccAGTTATCTTCCACGAAACGGCTGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGCGAGAGGTTc > 2:435995/1‑251 (MQ=255) ccAGTTAACTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCGGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTTGGGCGACGAGAAGGCTGAACATAAGGGGCCGCCAGCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTc < 2:490619/251‑1 (MQ=255) gTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCGTTCAGAGAACCGAGGTACAGCGCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCcg < 2:464540/251‑1 (MQ=255) tCCACGAAACGGATGTCGGGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATAGTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGGTTTCGCCGGAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCTGTTCTGTCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGcc < 1:283754/248‑1 (MQ=255) ccACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACt > 1:426898/1‑251 (MQ=255) ccACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACt < 1:94986/251‑1 (MQ=255) cACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACtt < 1:269449/251‑1 (MQ=255) aCGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGAAGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATgg > 1:83351/1‑250 (MQ=255) gTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTGCCCGCGCCGACTTCCATGTCCAATGGtt < 2:215513/251‑1 (MQ=255) gAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCATGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTAGCCGTAGCG‑ACCATCGGTCGGGAGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCACGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTg > 1:547570/1‑250 (MQ=255) gTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACaag < 2:193746/251‑2 (MQ=255) tctggTCCATACCAAGCTCTTTCAGAGGCCCGAAGTACAGCTCCTGAATAATGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTCGTGCTGTAAACGGTTGGGGATTTCGCCGTAGCG‑AACATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAACCTCCATAGGTTCTGGCCCCAGCGCGCGCAGCCAGGCAATTGGGTGAGAGGTTCCCGCGCCCACTTCCATGTCCAATGGTTGAACAAtt > 2:382749/4‑250 (MQ=255) gTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGc > 1:64169/1‑251 (MQ=255) ccATGCCCAGCACTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGGAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTACCATGTCCAATGGTTGAACAATGGTGCAg < 2:138360/251‑1 (MQ=255) cATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGc < 1:114944/251‑1 (MQ=255) aTGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGcc > 2:555558/1‑251 (MQ=255) cccAGCTCGTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTg < 1:49212/251‑1 (MQ=255) gttCATAGAACCGAGGGACGTCTCCGTAATATTGTCCTGCTATGGCTTAATGACCACCTGGAACTGATAGTTGTGCTGTAATCGGTTGGGGGTTTCGCCGTAGCT‑ACCATCGGTCAGGCGACGAGAATGCTGAACATTAGCATCCGCTAGCGGTTCGGGCGCCAGCGCGCGCATACCGGTCATTGGGTGAGAGGTTCCCGCGCCGTCGTCCAAGGCCAATGTTCGAACAATGGTGTAGCCCGGTCGAGccc < 2:509483/250‑1 (MQ=255) ttCAGAGAACCGAGGTACAGCTCCTGAAGATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGc < 1:112499/248‑1 (MQ=255) tCAGAGAACCGAGGTTCAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTTATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCGGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGt < 1:326593/193‑1 (MQ=255) tCAGAGAACCGAGGTACAACTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAAAGGTTGTGGTTTTCGCCGTCGCG‑ACCATCGGTCGAGCGACTAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGATAGGt > 2:326593/1‑193 (MQ=255) aagaACCGAGGTGCAGCTCCTGAATATTGTCCGGCGATGGCATAATGACCACCGGGAACTGATAGTATTACTGTAAACGGTTGTGGTTGTCCCCGTAGCG‑ACCAACGGTCGGGTGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCGGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCTCGCCGACTTCCATGGCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTaa < 2:506437/250‑1 (MQ=255) agaACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAAt > 1:255652/1‑251 (MQ=255) aGGTTCAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTaa < 1:69500/251‑1 (MQ=255) gTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑gg < 1:190446/251‑1 (MQ=255) cAGCTGCTGAATATTGTCCGGCGATGGCTTAATGACCCCCGGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTACGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCTCAGTAATCCTGTAA‑GGTCa < 2:101415/251‑1 (MQ=255) atagtGTCCGGCGATGGCTTAATGACCCCCTGGAACTGATAGTAGTGCTGGAAACGGGTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GTTCAGGATCAAGcc < 1:450463/247‑1 (MQ=255) atTGGCCGGCGAGGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGccc < 2:292263/250‑1 (MQ=255) ttGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATg < 2:427696/190‑1 (MQ=255) ttGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATg > 1:427696/1‑190 (MQ=255) ggCGATGGCTTAATGACCACCTGGAACTGCTAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGt > 1:402524/1‑251 (MQ=255) gCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTc > 1:33640/1‑251 (MQ=255) ggCTTAATGACCTCCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCT‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GTTCATGATCAAGCCCTGGAAGGTCCTgg < 2:469949/250‑1 (MQ=255) aaTGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCaa < 2:100812/251‑1 (MQ=255) aaTGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGGCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCGGGAAGGTCCTGGTATCa < 2:113472/250‑1 (MQ=255) ccaccTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACttt < 1:555558/251‑1 (MQ=255) ccTGGAACTGATAGTAGTGATGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACAATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGCCTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCAAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAACCCTGGAAGGGCCTGGTTTCAAACTTTTGc > 2:87970/1‑251 (MQ=255) ggAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCTGAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCat < 2:426898/250‑1 (MQ=255) ggAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCata < 2:64169/251‑1 (MQ=255) ggAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCat > 1:189254/1‑250 (MQ=255) cTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCAtattat > 1:409293/1‑251 (MQ=255) tGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATattatt > 1:515883/1‑251 (MQ=255) gATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATattatt < 1:477305/250‑1 (MQ=255) gATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGACAGGATCACGCACTGGAAGGTCCTGGTATCAAACTTTTGCATATTAttt > 1:541662/1‑251 (MQ=255) gcaaaCGGTCGGTGTTTCCGCCCTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACATTTCATTGGGTGAGAGGTTCCCGCGCCTACTGCTATGTCTAATGGTTGAACAATGGTGCAGCCCTGGTGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGGAGGTCCTGGTACCAAACTTTTTCATATTATTTCGGGCTTGATAc < 1:450837/249‑1 (MQ=255) gTAAAGGGTGGGGGTTTTCGCC‑TAGGGGACCAGCGGTCGGGCGACGAAAAGGCGGAACATAGGCGGCGGCCACCGGTTCGGGCCCAGGGGCGGGCAGACAGGTCATTGGGTGAGAGGTTCCGGCGCCGACTTCCATGTCCAATGGTGGAACAATGGTGCAGCCCGGGCGAGCCCAGTAA‑CCCGTAAAGTTCAG‑ATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATAc < 1:171724/250‑1 (MQ=255) aaaCGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACgg > 1:14853/1‑250 (MQ=255) ggTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTgg > 1:506848/1‑251 (MQ=255) gTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGa < 1:374236/251‑1 (MQ=255) ggtgtCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCGGAACAAAAGCAGCCGCAATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTTA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTGGCATATTATTTCGTGCTGGATTCGTGTGGATTTaaa < 2:111087/247‑1 (MQ=255) cccGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGTTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTTGAAGGTCCTGGTATCAAACTTTTGCATATTATTGCGTGCTGGATACGTGTGGATTTAaaggaagg < 1:383461/250‑1 (MQ=255) gTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGAt > 2:127972/1‑251 (MQ=255) aGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATc < 1:23839/250‑1 (MQ=255) aCCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGCTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCAAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTCTTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGtat > 1:290448/1‑251 (MQ=255) cggtcggGGGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATAGTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCg < 2:56210/251‑1 (MQ=255) gcggggggcgaGGAGGGTGAACATAAGCAGCCGGCATCGGGTTTGGCCCCCGCGGGCGCAGACAGGTCATTGGGTGAGAGGGTCCCGCGCCGGCTTCCATGTCCAATGGTTGAACAATGGTGCAGGCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCCAGCCCTGGAAGGTCCTGGTATTAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtg < 2:29145/243‑1 (MQ=255) gggCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtgga < 2:33640/251‑1 (MQ=255) cgaAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCACCAGCGCGCGCAGCCAGGTCATTGCGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCACACTTTTGCATATTATTTCGTGCTGGCTACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCtggattga > 2:508025/2‑244 (MQ=255) aaCATAAGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGCGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTAc > 2:30385/1‑251 (MQ=255) aaGCAGCCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAAc > 1:117105/1‑251 (MQ=255) gcagcCGCCATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGCACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACgg > 1:363727/1‑251 (MQ=255) agcCGGCATAGGTTCTGCCCACAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCGGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGGCCTGGTATCAAACTTATGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGAACAGTATCCCCGCtggatggagtagattcagtacgagagggg > 2:339947/1‑229 (MQ=255) gccgccATCGGTTCTGGCCCCAGCGCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGa > 1:496334/1‑250 (MQ=255) gccgcaATCGGTTCTGGCCCCAGGGCGCGCAGACAGGTCATTGGGGGAGAGGTGCCCGCGACGACTTCCATGTCCAATGGTTGACCAATGGTGCAGCCCTGGCGAGCCCAGTAATCATGTAA‑TGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACGTTAGAATATTAGTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGaa < 2:165330/251‑1 (MQ=255) gccATCGGTCCTGGCCACAGCGCGCGCAGCCAGGTCATTGGGTGCGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGCGCAGCCATGGCGAGCCCAGTCATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATCCGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATAAAGTACGAAACGGGaaaa > 2:43497/1‑250 (MQ=255) cAGCGCGCGAAGACCGGTCATTGGGGGAGAGGTTCCCGCGCCGACTTCCATGTCCCATGGCTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGGCAGGATCAAGCCCTGGAAGGTCCTGGTATCAACCTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAGGGAATCAGTATACCAGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGAGCTTAACGCATg > 2:528432/1‑251 (MQ=255) | CCAGGCACCCAGCGTCGGGTTTTCCCAGTTATCTTCCACGAAACGGATGTCGTGAATAGTCGGGTCCATGCCCAGCTCTTTCAGAGAACCGAGGTACAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCG‑ACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAA‑GGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTATTTCGTGCTGGATACGTGTGGATTTAAAGGAAGGGATCAGTATACCCGCTGGATGGAAGATATACAGTACGAAACGGGAAAAAGCAGGGCTTAACGCATG > NC_000913/3724932‑3725422 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |