Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,537	A→G	L78P (CTG→CCG)	recA ←	DNA recombination/repair protein RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,537	0	A	G	100.0%	207.8 / NA	70	L78P (CTG→CCG)	recA	DNA recombination/repair protein RecA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/35);  total (35/35)

GATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCT  >  NC_000913/2823290‑2823779                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   gATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGATTTACCTTCACGCTGCGCTGCGGCGATCACCTgcggcg                                                                                                                                                                                                                                                 >  1:215898/1‑251 (MQ=255)     tcgtcTTCGATTTCCGCCTTCGGCGTCAGGGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGAGTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGcggcgtca                                                                                                                                                                                                                                              <  2:444307/246‑1 (MQ=255)      cGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGCCGATCACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCCCGCTGCGCTGCGGCGATCACCTGcggcgtccgc                                                                                                                                                                                                                                            >  2:335288/1‑248 (MQ=255)                       ttCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCTCAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCTTAGATTGGTTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCTGGGTTTTACCGGaa                                                                                                                                                                                                                             <  2:112551/249‑1 (MQ=255)                              gcAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGt                                                                                                                                                                                                                    <  2:454756/250‑1 (MQ=255)                                aGTGCCGCCTCGGAGTCAACGACTATAACGTCGACTGCGCCAGAACGCGCCAGGGCGTCACAGAGTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTGTACGTGCGTAGATTTGGTCCAGCGCGTGTTCAGCAGCGATAAACGCACAGGTTTTACCTTCACGCTGCGCGGCGGCGAGCACCTGCGGCGTCATCGTGGTTTTACCGGAAGGTTCCGGTcc                                                                                                                                                                                                                  <  2:368921/251‑1 (MQ=255)                                 gTGCCGCCGCGGAGTCACCGACGCTACCGTCTACGACGCCAGAACGCGCCAGGGAGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCGGGGAGCACAGCAGGTTGTCGGTATCTACGCCCAGATTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCTTCAGCGTGGTTTTACCGGAAGATTCCGGTcct                                                                                                                                                                                                                 <  2:11740/251‑2 (MQ=255)                                   gccgccACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCCGCGTGGTTTTACCGGAAGATTCCGGTCCGt                                                                                                                                                                                                                >  2:117822/1‑250 (MQ=255)                                    ccgccACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGt                                                                                                                                                                                                                >  1:38426/1‑249 (MQ=255)                                                cAACGAAGATCAGGTCTAGTGCGCCAGAACGCGCAAGGGCGTCACCGATTTCCAGTGCCTGCGCGCCGGAGTCCGGCTGGAAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGGCCCGCGAGAGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGAGGCGATCACCTGCGGCATCAGCGTGGTTTTACCGGCCGATTCCGGTcaggcgatttctacg                                                                                                                                                                                                     >  2:320258/1‑234 (MQ=255)                                                 aaCGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACTGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATAc                                                                                                                                                                                                 <  2:354837/251‑1 (MQ=255)                                                  acgacgATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATGCCGGTCCGTAGATGTCGACGATACg                                                                                                                                                                                                <  2:288653/251‑1 (MQ=255)                                                       gaTAACGTCTACTGCGCCAGAACTCGCCAGTGCGTCGCAGATTTCCAGGGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATAGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGATTTTACCTGAAGATGCCGGTCCGTAGATTTCGACGATACGGccc                                                                                                                                                                                            >  2:202441/1‑250 (MQ=255)                                                        aTAACGTCTCCTGCGCCAGACAGCGCAAGGCCGTCACAGATTTCCAGTCACTGCTCGCCGGTGTCCGGCTGGGAGCAAAGCAGGTTGTACATATCGACGCCCCGTTTACGTGCGTAGATTGGGTCAAGCGCGCGTTCCGCATCGATAAACGCCCAGGTTTTACCTTCACGCTGCGCTGCGACGATCACATGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGAAGATTTCGACGATCCGGCCCAt                                                                                                                                                                                          >  2:534063/1‑251 (MQ=255)                                                                 aCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAg                                                                                                                                                                                    <  1:386115/248‑1 (MQ=255)                                                                    gcgcCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGacca                                                                                                                                                                                <  1:410606/249‑1 (MQ=255)                                                                     cgcCAGAACGCGCCATGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCt                                                                                                                                                                             <  1:420913/251‑1 (MQ=255)                                                                            aCGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCaa                                                                                                                                                                      <  1:432257/251‑1 (MQ=255)                                                                               cgcCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCTGTGGCCGGCTGGGAGCACAGCAGGTTGTCGATATCTACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGGTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgcg                                                                                                                                                                   <  2:463887/251‑1 (MQ=255)                                                                                     ggCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACACCCAGTTTACGTGCGAAGATTGGGGCCAGCGCGTGTTCAGCATCGATAAACGCCCAGGCTTTACCTTCACGCTGCGCTCCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGCTTTCGACGATACGGCCCATCGGACGACCACCTGCCCCAAGCGCGatat                                                                                                                                                             >  1:521098/1‑251 (MQ=255)                                                                                          cacaGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAg                                                                                                                                                         <  2:38426/250‑1 (MQ=255)                                                                                                       tGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACACCCTGCCCCAAGCGCGATCTCCAGTGAAAGCGacacgg                                                                                                                                           >  2:32582/1‑246 (MQ=255)                                                                                                        gCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAg                                                                                                                                                   <  2:181269/242‑1 (MQ=255)                                                                                                        gCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAg                                                                                                                                                   >  1:181269/1‑242 (MQ=255)                                                                                                        gCCTGCTCGCCGGTGGCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGACCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGt                                                                                                                                          <  1:318728/251‑1 (MQ=255)                                                                                                              tCGCCGGTGTCCGGCTGGCAGCACCGCAGGCTGTCGATATCGACGCCCAGTTTACGTGAGCAGCTTGGGTCCAGCGCGAGCTCCGCCTCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTCCCGCAAGACTCCGGTCCGTAGATTTCGACGCTACGGCCCACCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAACGCGAACCGGTagag                                                                                                                                      >  2:211008/1‑249 (MQ=255)                                                                                                               cGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGAGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGAt                                                                                                                                    <  1:138873/250‑1 (MQ=255)                                                                                                                gCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCAACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATgg                                                                                                                                  <  2:413707/251‑1 (MQ=255)                                                                                                                 ccGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGt                                                                                                                                 >  1:245635/1‑251 (MQ=255)                                                                                                                  cGGTGTCCGGCTGTGAGCACGGCAGGTTTTCGGTATCGACGCCGATTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGGCGATGCGGCCCAGCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGtt                                                                                                                                <  2:507916/251‑1 (MQ=255)                                                                                                                   ggTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGttt                                                                                                                               >  1:555812/1‑251 (MQ=255)                                                                                                                             ggggAGCGCAGCAGGTTGTCGATGTCGACGCCCAGTTGACGTGCGTAGATTGGGTCCAGCGCGGGTTCAGCAGCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGGTCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCGCCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCAt                                                                                                                     <  2:449195/250‑1 (MQ=255)                                                                                                                                gAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGa                                                                                                                  <  2:415014/251‑1 (MQ=255)                                                                                                                                    ccagcagGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACgg                                                                                                              <  1:32582/250‑1 (MQ=255)                                                                                                                                     cagcagGTTGGCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCTATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGt                                                                                                             <  2:48885/251‑1 (MQ=255)                                                                                                                                       gcagGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCCCCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGAGTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTGTCCACATCCATGGAACGGTCt                                                                                                           <  2:234622/251‑1 (MQ=255)                                                                                                                                          ggTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATAGAACGGTCTTcc                                                                                                        >  2:318375/1‑250 (MQ=255)                                                                                                                                                 gATATCGACTCCCAGGTTACGGTCGTAGATTGGGTCCAGAGTGTGTTCATCTGCGATAAACTCACATGTGTTACCGTCACGCTGCGCGGCGGCGATCACCTGCGGCGTCGGCGTGGTTGTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCTCCTGCCCCAAGCGCGATATCCAGTGTTAGCGAACCGGGAGATATGGTTTCCACATCCATGGAACGGTCTTCACCCAGgc                                                                                                 <  2:569443/251‑1 (MQ=255)                                                                                                                                                 gATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGgc                                                                                                 <  2:115663/251‑1 (MQ=255)                                                                                                                                                   tatCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCCGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCCTGGAACGGTCTTCACCCAGgcgc                                                                                               >  2:434184/1‑251 (MQ=255)                                                                                                                                                         cGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCatgatg                                                                                         <  1:335733/251‑1 (MQ=255)                                                                                                                                                          gCCCAGTTTACGTGCGTAGATTGGTTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTGCCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGGCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATgg                                                                                        <  2:215898/251‑1 (MQ=255)                                                                                                                                                            ccAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAg                                                                                      <  2:555812/251‑1 (MQ=255)                                                                                                                                                                     gTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTAcc                                                                              >  2:233275/1‑250 (MQ=255)                                                                                                                                                                      tGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCACGCGCGATATCCAGTGACAGCGAACAGGTAGAGATGGTTTCCACATCCCTGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCa                                                                             >  2:147912/1‑250 (MQ=255)                                                                                                                                                                          tAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTCCCAAATTg                                                                        >  2:479387/1‑251 (MQ=255)                                                                                                                                                                            gATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCCGGCGCATGATGGAGCCTTTACCAAATTGtt                                                                      >  1:490998/1‑251 (MQ=255)                                                                                                                                                                            gATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGtt                                                                      <  1:233275/251‑1 (MQ=255)                                                                                                                                                                                    ccAGCGCGTGTTCAGAATCGATAAACGCACAGGATTTACCTTCACGCTGAGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGACCTCCAGTGAAAACGAACCGGAAGAGCTGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGAATTTACCAAATTGTTTCTCagac                                                              >  1:86915/1‑248 (MQ=255)                                                                                                                                                                                         gcgTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGCTACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACCTCCATGGAACGGTCTTCACCCCGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGcc                                                         >  2:224758/1‑251 (MQ=255)                                                                                                                                                                                           gtgtTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCCATCTGGccc                                                        >  1:105912/1‑250 (MQ=255)                                                                                                                                                                                             gtTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGCTACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGCACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGt                                                     >  1:152984/1‑251 (MQ=255)                                                                                                                                                                                                   cATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTgcc                                               >  2:247028/1‑251 (MQ=255)                                                                                                                                                                                                    aTCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCCCCTGCCCCACGCGCGATATCCAGTGAAAGCGAACCGGCAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACAACATTGTTTCTCAATCTGGCCCAGTGCTgccg                                              >  2:112622/1‑251 (MQ=255)                                                                                                                                                                                                    aTCGATAAACGAACAGGTTTTACCTTCACGCTGCGCTGCGGCGCTCCCCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATACAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTgccc                                              >  2:497627/1‑250 (MQ=255)                                                                                                                                                                                                      cGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGCTCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTgccgcc                                            >  2:31882/1‑251 (MQ=255)                                                                                                                                                                                                       gATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTgccgcc                                            >  2:75704/1‑250 (MQ=255)                                                                                                                                                                                                            aCGCACGGGCTTTACCTTCACGCTGCGCTACGGCAATCACCTGCGGCGTCAGCGAGGTTTTCCCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCCGACCAACTGCCCCAAGCGCGATATCCCGTGAAAGCGAACAGGTAGAGATGGTTTCCACATCAATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGACCAGTGCAGCCGCCAACGCt                                      >  2:378834/1‑251 (MQ=255)                                                                                                                                                                                                              gcccaGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCttt                                    <  2:452441/248‑1 (MQ=255)                                                                                                                                                                                                                 caGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCtg                                 <  1:304299/251‑1 (MQ=255)                                                                                                                                                                                                                  aGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCtgt                                >  1:559044/1‑251 (MQ=255)                                                                                                                                                                                                                       ttACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCtgtttgtt                           >  2:209047/1‑251 (MQ=255)                                                                                                                                                                                                                       ttACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCACATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGCACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCCCCGCTTTCtgtttgtt                           >  1:560296/1‑251 (MQ=255)                                                                                                                                                                                                                         aCCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGtttt                         <  1:75704/251‑1 (MQ=255)                                                                                                                                                                                                                             tCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAAGCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTtcgt                      <  1:335288/250‑1 (MQ=255)                                                                                                                                                                                                                                 gctgcgctgcGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCAATCGGCAGACCACCTGCCCCAACCGCGAAATCCAGTGACAGCGAACCGGTACAGAAGGCTTCCACATCCATGGAACAGTCTTCACCCAGGCGCATGATGGCGCCTTTAACAAATTGTTTCTCAATCTGGCCCAGACCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGAt                  >  1:56888/1‑250 (MQ=255)                                                                                                                                                                                                                                      gctgcgGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCAt            <  1:434184/251‑1 (MQ=255)                                                                                                                                                                                                                                          cggcgATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCAttttt        >  1:378033/1‑251 (MQ=255)                                                                                                                                                                                                                                              gATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTc    >  1:547395/1‑251 (MQ=255)                                                                                                                                                                                                                                               aTCACCTGCGACGTGAGAGTGGTTTTACCGGAAGCATCCGGTCCGCAGATCTCGACGCAACGGCCAATCGGCAGACCCCCTGCCCCAAGCGCGATATACAGTGAAAACGAACCGGAAGAGATGGTTTACACATCCATGGAACGCTATTCCCCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCCGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGCTAGCCCTTTTTAATcc   >  2:90753/1‑251 (MQ=255)                                                                                                                                                                                                                                                tCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGCCCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCt  >  1:529773/1‑251 (MQ=255)                                                                                                                                                                                                                                                 cACCTGCGGCTTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTGCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGGTTCCACATCCATGGAACGGTCTTCTCCCAGTCGCATGATGGAGCCTTTACCAAATGGTGTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCt  <  1:275791/250‑1 (MQ=255)                                                                                                                                                                                                                                                 cACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGGCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATGGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCt  <  1:247099/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   GATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCT  >  NC_000913/2823290‑2823779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑