| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,358,859 | T→C | Y110C (TAT→TGT) | puuP ← | putrescine:H(+) symporter PuuP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,358,859 | 0 | T | C | 97.1% | 106.9 / ‑6.1 | 35 | Y110C (TAT→TGT) | puuP | putrescine:H(+) symporter PuuP |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (16/18); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCAT > NC_000913/1358612‑1359103 | ccAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAAt < 1:4078/251‑1 (MQ=255) tctcCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAAGATACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGcc > 1:289620/1‑251 (MQ=255) gAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGt < 1:405404/249‑1 (MQ=255) gATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCCACCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGGGGGAAAGATTTTCGCCCACAACACGTTAATCATCGGCCAAAAAAGACAATCCAACCGCGCAT‑ACCAGCCGACCCTAAAACCGACGGACTGGTTAATCTACt > 1:15419/1‑251 (MQ=255) gatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGGCGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGGCCATAAATCCGACGTGCTGGTTAATCGGCTTTTGCGCGGAGGta < 2:266213/250‑1 (MQ=255) ggAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACAGCCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAg < 1:182042/250‑1 (MQ=255) tGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAAc < 1:514262/250‑1 (MQ=255) cACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTTAAATGGGGACGAAGGGTACCACCCCCACCCACCGCGGCACTTTCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGCCCATAAATCCGACGTGCTGGTTAATCGCCTTTTTGGCGTGGGAATAGGCCGGACCgg > 2:468126/1‑251 (MQ=255) gTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGtt < 1:80143/251‑1 (MQ=255) caaGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCCCCCACACCCACGGCGGCTCTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCACCAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAAGCCGACGTGCTGGTTAAGCGCCTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAg < 2:450365/249‑1 (MQ=255) ttGACGCTCTTCAGATTCGCGGCGGTTAAAATTGCGACGAAGGGTACCCCCCACAACCACGGCGGGACTTCCGGGAAGAGGGCGGAGAGAAAGATTTTCGCCAACAAGACGGTAATCAACTGGAAAAAAAGACAATCCAACAGCG‑ATGACCAGACGACCATAAATCCGACGTGCTGGTTAAACGACTTTTGCGCGTTGGTAAAAGCCGAACCGGCCTCCGGGAACTGGCGAACCCGATTGCCGTAGGTGa > 2:312569/1‑250 (MQ=255) aGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAg < 1:531599/251‑1 (MQ=255) cgcgGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCTTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCacac < 1:240707/251‑1 (MQ=255) ggcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACAcccc > 1:92756/1‑250 (MQ=255) aaTGGCGACGAAGGTTAACACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcgcc > 1:170767/1‑250 (MQ=255) acgaAGGTTACCACCCACACCAACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGCTTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTTGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGCCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAAt > 2:289458/1‑250 (MQ=255) cgaAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGCTTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAAt > 2:163180/1‑249 (MQ=255) cgaAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAAt > 1:360264/1‑249 (MQ=255) ccacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCAAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACACGCATCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGCTAGCGGTAAAACGCAAACCCGCCAGCGCCAGCAAATAGGACGCCGGAACgg > 1:371335/1‑250 (MQ=255) ccgccccgcggccatcccGGGAAGAGGGCGGAGAGATAGATTTCCGCCAAAAATACGTGAATCCTCGGAAAAAAGGTACATTCCAGCTGCG‑AGGCCCAGCCGACCAAAAAGCCGACGCGCTGGTAACTCGAGTTGGGCGGGTAGGTATCGGCCGACCCGGCCTCGGGAAACTGGCGAACAATTTGGCCGGAGCTGAGAGCGGAAACCACCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGAcc < 2:459664/235‑1 (MQ=255) gcggcACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTAAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTa < 1:43381/251‑1 (MQ=255) ccGGGAAGAGGGGGGAGAGATAGATTTTCCCCAACAAGACGTAAATCATCGGAAAAAAGAGACAATCCAGCAGCT‑ATGCCCAGCCGACCATAAATCCGGCGGGCTGGTTAATCGACTTTTGCGCGGAGGTATAGGCCGAACCGGCCTCCGGAAACTGTCGAACCAGTTTGCCGGGGCTGATAGCGGTAAACAGCACACCCGCCAGCTCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGgag < 1:280632/251‑1 (MQ=255) gAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCg < 2:89190/146‑1 (MQ=255) gAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCg > 1:89190/1‑146 (MQ=255) gagagagaGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGCCCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAACCTGGCGAACCAGTTTGTCGTAGTTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGt < 1:163180/251‑1 (MQ=255) ggagaAAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGTTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGACCCGGCCTCCGGAAACGGGCGAACCATTTTGCCGGAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTa < 1:417691/250‑1 (MQ=255) atatattttctcaaaaaaGACGTTAACCATCGGCAAAAAGATATAACCCAGCAGCG‑ATGACCAGCCGACAATAAATCCGAGGGGTTGGTTAATCGACTTTTGCGCGTAGGTTAAGGCCGAACCGGCCTCCGGAATCTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGGTAATGCTGGAGACAATACCAAAAGTATCaa < 1:161728/235‑1 (MQ=255) ttttCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACCATACCAAAAGTATCAAATACg > 2:549444/1‑251 (MQ=255) gCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAACCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCAt < 1:218748/251‑1 (MQ=255) ccAACAAGACGTTAATCATCGGCGAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGCCGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCACAAGTATCAAATACGGCCATc > 2:74964/1‑251 (MQ=255) aCGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGCCGCCGGAACGTGACCGTCGCTAATGCCGGAGAc > 2:381869/1‑215 (MQ=255) aCGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACAATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCCCACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGAc < 1:381869/215‑1 (MQ=255) atcatcGGCAAAAAGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGCACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGc > 1:92451/1‑251 (MQ=255) ggaaaaaaGAGACAATCCAGCAGCG‑AGGACCAGCCGCCAAAAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGAcc < 1:53917/248‑1 (MQ=255) aaGAGACAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcatcat > 1:191498/1‑251 (MQ=255) | CCAGACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCG‑ATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATCAT > NC_000913/1358612‑1359103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |