Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,189,980	A→G	T156T (ACT→ACC)	phoP ←	DNA‑binding transcriptional dual regulator PhoP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,189,980	0	A	G	97.8%	158.4 / NA	46	T156T (ACT→ACC)	phoP	DNA‑binding transcriptional dual regulator PhoP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (25/19);  minor base T (1/0);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTCACGGGCGG  >  NC_000913/1189734‑1190212                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          cccGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCCGCTCCGCATCCGGATAGAGTTGGAGCATTAAAGAATCTTTGCTGACCACTTTGCCATTATTGGGTAGCAACGCTTCCATAATTgtgt                                                                                                                                                                                                                                       >  2:400838/1‑251 (MQ=255)   cGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑At                                                                                                                                                                                                                                    <  2:171899/251‑1 (MQ=255)     cAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCCGCGCAATTCGAACAGATAGACCTGGCCGCGAAAGGTGGTAATCACTTATTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTAAATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTc                                                                                                                                                                                                                                  >  2:552726/1‑251 (MQ=255)           gagCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTGTGCCATTATTGCGTATCAACGTTTCCAGAATGGTGT‑ATTCGAAcgc                                                                                                                                                                                                                            <  1:383431/251‑1 (MQ=255)                                       ttCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAAt                                                                                                                                                                                                 >  1:467128/1‑250 (MQ=255)                                         catcaGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATACGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAAt                                                                                                                                                                                                 >  2:385164/1‑248 (MQ=255)                                            caGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTTGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCTGTCAGTTTGATCACTTCGTCATTAATAGATa                                                                                                                                                                                            <  2:316822/250‑1 (MQ=255)                                                 cAATTCGAACAGATAGCCCTTGCCTCGAACGGTGGTAATCACTTCGTGGAGATATTGTGTCGGAATTTTTTTGCGCAGACGTCCCATCAGTACAGCAATGGTATGGCTTTCCCGCAGCGCGGCATTCGGATATAGTTAGAGCATTAACGAATCGTTGCTGATCACTTGGCCATGAGTTCTTATCAACGTTTCCTTAAT‑GTGTTATTCGAACGCGGTCAGGATGATCATTTCGTCacaaagatacaattca                                                                                                                                                                                       <  2:525704/250‑16 (MQ=255)                                                  aaTTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACg                                                                                                                                                                                     >  2:17859/1‑251 (MQ=255)                                                                                      aTCACTTCTTGGGGATAATGTGCCTGAATTTTTTTGCGCAGCCGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGATGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAAt                                                                                                                                                 >  1:125332/1‑251 (MQ=255)                                                                                               tGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCtgtg                                                                                                                                         >  2:150300/1‑250 (MQ=255)                                                                                                  ggATATTGTGCCTGAAGTTTTTTGCGCAGACGTCCCAGCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCAGTAACGAAGCGTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGAGCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGc                                                                                                                                     <  1:68705/251‑1 (MQ=255)                                                                                                        tgtgCCTGAATTTTATTGCGCATACGTCCCATCAGTACATCAGTTGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCAATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTACTAGATAATTCACGGCGAGAGAGATCAACCTGAAACGTGGGGAGCGCAATTACCTGTGAAGCCCGAcc                                                                                                                               >  2:534132/1‑251 (MQ=255)                                                                                                            ccTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGCATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCt                                                                                                                            >  1:542593/1‑250 (MQ=255)                                                                                                                       ttGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTAcgccgcct                                                                                                                >  2:555194/1‑249 (MQ=255)                                                                                                                             aGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATGACGCCGCAGTAATGc                                                                                                          <  2:347830/251‑1 (MQ=255)                                                                                                                              gACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCCGCACCGCATCCGGATAGAGTTGTAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGACCGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGcc                                                                                                         >  2:96509/1‑251 (MQ=255)                                                                                                                                 gTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCGCTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTAGTACGCCGCATTAATGCCTGc                                                                                                      <  2:294625/251‑1 (MQ=255)                                                                                                                                     cATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTc                                                                                                  <  2:467128/251‑1 (MQ=255)                                                                                                                                        cAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTcgcg                                                                                               >  2:330047/1‑251 (MQ=255)                                                                                                                                              aTCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATc                                                                                          >  1:430493/1‑250 (MQ=255)                                                                                                                                                cAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCAcc                                                                                       >  2:429540/1‑251 (MQ=255)                                                                                                                                                   tGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACctct                                                                                    >  1:444898/1‑251 (MQ=255)                                                                                                                                                      tATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTc                                                                                  >  2:341509/1‑250 (MQ=255)                                                                                                                                                       aTGGCCTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCa                                                                                 >  1:222053/1‑250 (MQ=255)                                                                                                                                                               cccGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCCTAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGTCGAGAGCGATCAACCTGAAACGGGGGGAGCGAAATGACCTTTAAAGCCAGACCGCTATAACGCCGCATAAATGCCTGCGTTCGCGCCATCACCTCTTCAATATGCAAc                                                                        >  1:383652/1‑251 (MQ=255)                                                                                                                                                                ccGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACg                                                                       <  1:353877/251‑1 (MQ=255)                                                                                                                                                                 cGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGACTCTTTGCTGACCACTTTGCCCTTATTGAGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGCGAGAGATCACCCTGCAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCTCCATCCCCTCTTCAATATGAAACgg                                                                      >  2:505828/1‑251 (MQ=255)                                                                                                                                                                   cAGCTCCGCATACTGATAGAGTTTGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCTCCTCTTCAATATGAAACGGtt                                                                    <  2:76759/251‑1 (MQ=255)                                                                                                                                                                          gCATCCGGATAGAGTTGGCGCATTAACGAATCTTTGCTGATCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATAACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCATACCGCTCTTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAggccc                                                             >  2:486852/1‑247 (MQ=255)                                                                                                                                                                           cATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTcaca                                                            <  1:13543/251‑1 (MQ=255)                                                                                                                                                                           cATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTcac                                                             >  1:192974/1‑250 (MQ=255)                                                                                                                                                                            aTCCGGATAGATTTGGAGAATTAACGCATCTTTGCTGCCCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCCTCACCTCTTCAATATGACACGGTTTAGTCACAt                                                           >  2:72626/1‑251 (MQ=255)                                                                                                                                                                                   tAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAatcatc                                                    >  1:6994/1‑251 (MQ=255)                                                                                                                                                                                    agagTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAatcatc                                                    >  1:92318/1‑250 (MQ=255)                                                                                                                                                                                     gagTTGGAGCATGAACGAATCTTTGTTGTCCAATTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑AGTAGAGCGAGGGGAGTGTGATCACTTCGTCATTAAGAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAAGGACCTGTGAAGCCAGAACGCTATTACGACGCATTAATGCCTGCATTCGCGCCATCAACTCTTCAATATGAAACGGTTGAGTCATATAATCATCAg                                                  <  2:383652/251‑1 (MQ=255)                                                                                                                                                                                       gTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACAGAATCATCAGCa                                                <  1:416756/251‑1 (MQ=255)                                                                                                                                                                                            aGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATg                                                                            <  2:182466/218‑1 (MQ=255)                                                                                                                                                                                            aGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATg                                                                            >  1:182466/1‑218 (MQ=255)                                                                                                                                                                                                           ttGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACtt                                <  1:57706/247‑1 (MQ=255)                                                                                                                                                                                                           ttGCTGACCACTGTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCATCACCGGCACTTAATACTTc                               <  1:199797/248‑1 (MQ=255)                                                                                                                                                                                                              cTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACttt                         >  2:430633/1‑251 (MQ=255)                                                                                                                                                                                                                gACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCAGGCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGt                       <  1:255325/251‑1 (MQ=255)                                                                                                                                                                                                                gACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATGCGAACGCGGTCAGGTTGATCACTTCGTCATTAATAGATAATTCACGGCTAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGCCCTGTGAAGCCAGACCGCTATTACTCCGCATTAATGCCTGCATTCGCGCCAGCACCTCGTCAATATGAAACGGTTTAGTCACATAATCATCAGCTCCGGCCATTAATACTTCGACttt                         <  2:377914/249‑1 (MQ=255)                                                                                                                                                                                                                       ttGCCATTATTGCGTATCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGc                  <  2:6994/249‑1 (MQ=255)                                                                                                                                                                                                                                      aTCAACGTTTCCATAATGGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAAGTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCCCCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTCACGGGCgg  <  2:461022/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          CCCGCAGCGAGAGCGGGAAAAAAAGACGCAGTAATTTTTTCATCAGCGCAATTCGAACAGATAGCCCTGGCCGCGAACGGTGGTAATCACTTCTTGGGGATATTGTGCCTGAATTTTTTTGCGCAGACGTCCCATCAGTACATCAATGGTATGGCTTTCCCGCAGCTCCGCATCCGGATAGAGTTGGAGCATTAACGAATCTTTGCTGACCACTTTGCCATTATTGCGTATCAACGTTTCCATAATAGTGT‑ATTCGAACGCGGTCAGTTTGATCACTTCGTCATTAATAGATAATTCACGGCGAGAGAGATCAACCTGAAACGGGGGGAGCGAAATGACCTGTGAAGCCAGACCGCTATTACGCCGCATTAATGCCTGCATTCGCGCCATCACCTCTTCAATATGAAACGGTTTAGTCACATAATCATCAGCACCGGCACTTAATACTTCGACTTTGTCCTGCCAGCTTTCACGGGCGG  >  NC_000913/1189734‑1190212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑