| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,429,128 | 0 | A | G | 54.4% | 23.6 / 81.7 | 69 | K27R (AAA→AGA) | stfR | putative prophage side tail fiber protein StfR |
| Reads supporting (aligned to +/- strand): ref base A (15/16); new base G (24/13); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCGACCGAATCCGTGGCCATTGATATTGCTTATGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGG > NC_000913/1428885‑1429376 | tccggcagcgtccgtcgttgttGATATTGCTTATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAAc > 2:110908/21‑251 (MQ=255) ccgaccgaATCCGTGGCCATTGATATTGCTTATGAAGGCCCCGGCAGTGGCGCCTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACAAAGTGTTATGACAGCCCGACGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCCGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCCAAACGTAAc > 1:564329/1‑250 (MQ=255) aTCCGGGGCCAGTGATATTGCTTATGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCGCAATCCAGCTGAAAGCAAAACGTAACAGCACCACg < 2:465789/251‑1 (MQ=255) gtcgttgttGATATTGCTTATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATTCAGCTGAAAGCCAGACGTCACAGCACCACggagg > 2:447235/8‑248 (MQ=255) cgttgttGATCTTGATTATGACGGCTCCGGCAGTGGCAACTGGCGTACTGTAGGATTACTCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCACGACGGACCCGGAGGGCTTTTTTGTGGGGGGAATATGGAAGTAAAGATTTCAGGCGACCTGACAGACGGCACAGGAAAACCGGCACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACCGCAACACggtg‑g‑tg > 2:381028/6‑251 (MQ=255) cgttgttGATATTGCTTATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACggtg‑g‑tg > 1:187014/6‑251 (MQ=255) tATGAAGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTggg < 1:334552/249‑2 (MQ=255) aTGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTc < 2:234528/251‑1 (MQ=255) aaGGCTCCGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCTCCACGGTG‑G‑TGGGGAACACGGTGGGCTCAGa < 1:160798/251‑1 (MQ=255) tccGGCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTgggctacgagaatc > 2:177645/2‑239 (MQ=255) cGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCg > 1:317236/1‑251 (MQ=255) ggCAGTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCgg > 1:346426/1‑251 (MQ=255) agttgCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTAGAAATTCTGATTAGCCAGGTAACCCAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATAGGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATg < 2:185104/247‑1 (MQ=255) gTGGCGACTGGCGTACTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCAACACGGTG‑GATGG‑GAACACGGTGGGCTCAGAGAATCCGGATgc > 1:495757/1‑250 (MQ=255) cggaCTGACGGATGCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAATCCGGGCGtt < 2:460696/248‑1 (MQ=255) gtaCTGACGGATTCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGtt > 1:175382/3‑250 (MQ=255) gACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTGCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGGAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTTAAAGCAAAACGTAACAGCACCACTGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATTAATCCGGTCGTTACAGc < 2:378134/250‑1 (MQ=255) ggattCATCGTTGGGGTCGGTTATAAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATgg > 1:64105/4‑251 (MQ=255) cGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGAGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGa < 1:139373/250‑1 (MQ=255) cGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAg < 2:171667/251‑1 (MQ=255) tataAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTAc < 1:110908/250‑1 (MQ=255) ttCTGATTAGCCAGGGAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGCCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGCACGGCCGGTACAgtgtcc > 2:315031/1‑246 (MQ=255) gATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTACCAGCACCACGGAG‑G‑TGGTGAACACGGTGGGCTAAGAGAATCCGGATGAAGACGGGCGTTACAGCATGGATGGGGAGGACGGTCAGTACAgtggcaccct > 2:349461/1‑242 (MQ=255) ttAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGt > 2:125431/1‑251 (MQ=255) ccgggTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCGTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAAGCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTTAGTACGGTCAGTACAGCGCTATTCTGTtggt < 2:481989/248‑1 (MQ=255) cAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAgtgtcatcctgcaggtt > 2:163313/1‑235 (MQ=255) aGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAgtgtcatcctgcaggttg < 1:163313/251‑18 (MQ=255) aaCACAGTGTGATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGGACAgtgtcatcctgcaggttgacg < 2:62126/250‑21 (MQ=255) acaGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAgtgtcatcctgcaggttgacggttt > 1:448740/1‑227 (MQ=255) gccgGTTCAGGCGGGTTTTTTTGGGGGGGGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCAAAGGAAAACCGGGACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGGGAACACGCTGGCCGCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGATTACGGTCATTACAGCGTTATTCTTTTGGTGGAAGGATTCCCGCCGTCACATGCCgg < 2:447809/251‑1 (MQ=255) gtccAGGCGGGCTTTTTTGGGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCGCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCGGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGAcc < 1:537475/248‑1 (MQ=255) gcgggtgggCTTTTGTGTGGGGGGAATAGGTCAGGAAAGATTTCAGTTGTACTTAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCGGCTGAAAGAAAAACGAAACAGCCCCA‑GGTGTG‑GGGTGAACGCGCTGGCTTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTAGGGTCATTTCAGCGGGATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCAt < 1:34094/245‑1 (MQ=255) ggCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTAc > 1:320049/1‑251 (MQ=255) gggCTTTTTTGTGGGGTGAAGATGGCAGTAAAGATTGCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGAAAAACGTAACAACACCACGGTG‑G‑TGGTTAACACGCTGGCCTCGGAAAATCCGGATGAACCCGGGCGTGACAGCATGGACGTTGAGTAGGGTAAGTACAGCGTTATTCTGTTGGTGGAAGTATTCCCGCCGTCACATGCCGGGACCATTACCgt < 1:369497/251‑1 (MQ=255) ggCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGCCCATTACCgtg > 2:744/1‑251 (MQ=255) gCTTTTTTGTGGGGTGAATATGGCAGTAAAGCTTTCAGGTGTACTGAAAGACGGCCCAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑AGGCGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTACCATGCCGGGACCATTCCCgtgt > 2:284960/1‑251 (MQ=255) tttGTGGGGTGAATATGGCAGTAAATATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCTCCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGTGCTCATAGAATCCGGATGAAGCCGGGCGGTACAGCATGGATGTGTTGTACGGTCAGTACAGTGTCATCCTGCGGGGTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGa < 1:43405/250‑1 (MQ=255) ttGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGaa < 1:366256/250‑1 (MQ=255) tgGGGTGAATATGGCAGTAAAGATTTCAGGTGTCCTGAAAGCCGGAACAGGAACACAGGCACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCAAGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACGGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCAAATGCCGGGACCATTACCGTGTATGAAGAtt > 2:561195/1‑251 (MQ=255) gggggTGAATAGGGCAGTAAAGATTTCAGGTGGACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAAACCAGATGAAAGCAAAACGTAACAGCACCGCGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAAACCGGTTGAAGCCGGGCGTTACAGCATGGAGGTGGAGTACGTTCAGTACAGCGTTATTTTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGGATGAAGAtg < 2:182601/250‑2 (MQ=255) gggTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACCGGAAAACCGGTAAAGAACTGCACCATTCAGCTGCAAGCCAGACGTAACAGCCCCACGGTG‑A‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGCGTACGGCAAGTACAGTGTCATCCTGCCGGCTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGCTtc > 2:493375/1‑250 (MQ=255) gggTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCAACATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGCTGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCAACGTGTATGAAGATtcc > 2:502073/1‑250 (MQ=255) gTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAACACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGCAGATtctc > 1:26086/1‑250 (MQ=255) tGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGACAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtcaca > 1:502116/1‑247 (MQ=255) aatGGCTGTAAAGATTGCAGGTTAACTGAAAGCCGGCACAGGAAAACCGGTACAGAACTGCAATACCCAGCGGAAAGCAAAACTGAAAAGAACCACGGTG‑G‑GGGTGAACACGCGGGCCTCAGAAAATCCGGAGGAAGCCGGGCGTTACAGCATTGACGTGGAGTCGGGTCAGTCCAGCGTTATTCTTTTGGTGAAATTATTCCCGCCGGCACATGCCGGGACCATTACCGTGTATGAAGATGCTCAACCCg < 2:510030/250‑1 (MQ=255) cAGTAAAGATTTCAGGTGTCCTGAAAGACGGCACAGGAAATCCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCCCCACGGTG‑G‑TGGTGACCCCGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTTTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACg < 1:137197/251‑1 (MQ=255) cAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccggggacg < 2:198451/251‑8 (MQ=255) tAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTg > 2:156236/1‑251 (MQ=255) aaaGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCAGCGCACGCCGGGACCATCACCGGGTATGAAGATTCACAAccgtggacgctga < 2:88738/251‑12 (MQ=255) gATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATg > 1:180217/1‑251 (MQ=255) tCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAttt > 1:517741/1‑251 (MQ=255) caggagtccTGAAAGACGGCACAGACAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCCGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGAtttt > 2:258665/9‑251 (MQ=255) ccTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCtgtg > 1:138193/2‑251 (MQ=255) gAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGc < 1:175182/249‑1 (MQ=255) gAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCa < 2:446098/251‑1 (MQ=255) gAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGTTGAACACGCTGGCCTCATAAAATCCGGAGGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGTTCAGTACGGCGTTATTCTGTTGGGGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGcc < 2:341157/250‑1 (MQ=255) aGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATg > 2:161128/1‑250 (MQ=255) cGGCACAGGCAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGCTTTTCTCGGTGCCATGACgg > 1:454630/1‑251 (MQ=255) cGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGCCgg > 1:220903/1‑251 (MQ=255) ggCAAAGGAAAACCGGTACAGAACTGCACAATCCAGATGAAAGAAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTAAAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACAGGCAGGGACCATTAACGTGTATGAAGATTCTCAACCCGGTAAGCTGAATGATTTTAACGGTGCCATGACgg > 1:279228/1‑250 (MQ=255) cacaGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACggagg > 1:19581/1‑251 (MQ=255) cGGTAAAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCCCCACGGTG‑G‑TGGTGAACACTTGGGCCTCAGAAAAGCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCGGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGt < 2:320049/251‑1 (MQ=255) ggTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCggc > 2:166983/1‑249 (MQ=255) cggccaaaTCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGTGCTTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCAc < 1:200182/245‑1 (MQ=255) cTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTG‑G‑TGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgc > 1:56678/1‑248 (MQ=255) cacaATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGCTACGCTGAATGATTTTATCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTgc > 2:4505/1‑251 (MQ=255) tCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGt > 1:191609/1‑251 (MQ=255) ccAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGtt > 1:188442/1‑251 (MQ=255) aaGCAAAACGTAACAGCACCACGGTG‑G‑GGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCTCTGCGCCGTTTTGCACTg > 2:456390/1‑251 (MQ=255) aaaaCGTAACAGCACCACGGTG‑G‑TGGTGCACACGCTGGCCTAAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGAtgg > 2:470524/1‑251 (MQ=255) | CCCGACCGAATCCGTGGCCATTGATATTGCTTATGAAGGCCCCGGCAGTGGCGACTGGCGCACTGACGGTTTCATCGTGGGTGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTG‑G‑TGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGG > NC_000913/1428885‑1429376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |