Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,349,111	IS2 (+) +5 bp	intergenic (‑367/+200)	dcuB ← / ← dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	36 (0.640)	36/490	0.5	100%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	= 4349115	0 (0.000)					intergenic (‑371/+200)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
*	?	NC_000913	4349111 =	2 (0.040)	55 (0.980)	48/492	0.2	96.4%	intergenic (‑367/+204)	dcuB/dcuR	anaerobic C4‑dicarboxylate transporter DcuB/DNA‑binding transcriptional activator DcuR
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

CGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCAT  >  NC_000913/4499267‑4499739                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     cGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgt                                                                                                                                                                                                                                <  1:267601/251‑1 (MQ=31) cGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACAA‑GCCGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGGCTGGAAATATAGGGGCAAATCCACCTtgt                                                                                                                                                                                                                                >  1:380814/1‑251 (MQ=14)  ggAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTtgtg                                                                                                                                                                                                                               >  1:327532/1‑251 (MQ=32)     gTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTg                                                                                                                                                                                                                            <  1:101291/251‑1 (MQ=33)           agaggatcGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCTTGCCTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGGTATAGCATTATAACGAATGGCAGCCGCAGAGTGCGCTGGGGTATCGCTCGCCACGGGAATATCTGCGGCAGCTGGCTTGTAATGGGTTAAGTTAGAACAGATGTCTGGAAATAAAGTTGCAAATCCACTCTGTGCTTATATGa                                                                                                                                                                                                                      <  2:504844/244‑1 (MQ=11)             aggatcGGAATAGCAGAGAGCGCCTTTCAAACGAGAAAGCGTGACTACATCAGTATCTAGCCCAAACCA‑GACGGGTGAACGGCAGCAAAGACGCGTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCGGTGGTATCGCACGCCACGGTAATATCTGCGGCAGCTGGCTTGTAATGGGTTAATTTATAACAGATGTCTGGACATATAGGGGCAAATCAACCTTGTTTTGATATGAAt                                                                                                                                                                                                                    <  2:364167/246‑1 (MQ=2)               tAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCAGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAGTAc                                                                                                                                                                                                                  >  1:176957/1‑251 (MQ=25)                   ggAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGat                                                                                                                                                                                                              <  1:41835/251‑1 (MQ=40)                        aGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCCTTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTAGAAATATAGGGGCAAATCCACCTTGTTCTGATATGAATACGGATAttt                                                                                                                                                                                                          <  2:120969/250‑1 (MQ=255)                            gagagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATAc                                                                                                                                                                                                     <  1:300521/251‑1 (MQ=255)                            gagagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGG‑TTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATAc                                                                                                                                                                                                     >  1:198210/1‑250 (MQ=255)                            gagagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCAGGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCTCCTTGTGCTGATATGAATACGGATATTTTATAc                                                                                                                                                                                                     <  1:174616/251‑1 (MQ=255)                             agagCTTCGTGAAAACGATAAAGCGTGACTACATCAGCATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGGTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACa                                                                                                                                                                                                    <  2:442342/251‑1 (MQ=255)                              gagCTTCGTGAAAACGAAACAGCCTGACTCCATCAGTATCATGCTCACACCA‑GACGGGTTAACGGCAGCAACGAACCTTGCAGAGGCGTTCGAGCATTATCACGAATGGCATACGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGCTGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATAc                                                                                                                                                                                                     >  2:559356/1‑249 (MQ=14)                                gCTTCGTGAAAACGATACAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACCGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGa                                                                                                                                                                                                  >  2:193694/1‑250 (MQ=255)                                   tCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATa                                                                                                                                                                                               <  2:288797/250‑1 (MQ=255)                                    cGTGAAAACGATAAAGCGTGACTACATCAGTGTCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACt                                                                                                                                                                                             <  1:174003/251‑1 (MQ=255)                                       gAAAACGATAAAGCGTGACTACAGCAGTATCATGCCCAAACCA‑GACTGGTGAACGGCAGAAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATTTCTGGAAATATAGGGTCAAATCCACCTTGTGCTGATATGGATACGGATATGTTATACAGAATACtttt                                                                                                                                                                                          <  2:465555/251‑1 (MQ=14)                                          aaCGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAg                                                                                                                                                                                       <  2:403562/251‑1 (MQ=255)                                           aCGATAAAGCGTGAATACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGCACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATATGCGGCAGCGGGCTTGTAATGGGTTAAGTGCTAACAGATGTCTGGAAATATAGGGGAAAATCAACCTTGTGCTGATATGAATACGGATATTTTATCAAGAATACTTTTCAgc                                                                                                                                                                                      >  2:463897/1‑250 (MQ=14)                                              aTAAAGCGTGACTGCATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACa                                                                                                                                                                                   <  1:369058/251‑1 (MQ=255)                                                   gCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTAGCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGa                                                                                                                                                                              <  1:121596/251‑1 (MQ=255)                                                     gTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACt                                                                                                                                                                            >  2:122324/1‑251 (MQ=255)                                                       gACTACAGCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGt                                                                                                                                                                          <  2:327532/251‑1 (MQ=255)                                                           aCATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTAt                                                                                                                                                                        <  1:525010/249‑1 (MQ=255)                                                                     cATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTCTAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGcc                                                                                                                                                             >  2:149556/1‑250 (MQ=255)                                                                      aTGCTCAAACCATGAGGGGT‑AACGGCAGCAAAGAACCTTGCAGAGGCGGTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTTGGTTATCGCTCGCCACGGGAATATCTGCGTCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTTCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAATCGACTATATTACGCTCTGCCat                                                                                                                                                           <  2:249670/251‑1 (MQ=17)                                                                      aTGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCAGCCGCATAGTGCGCCTGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCat                                                                                                                                                           <  1:297470/251‑1 (MQ=255)                                                                            aaaCCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCTGTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATTTCTGGAAATATAGTGTCAAATCCCCCTTGTGCTTATATGTATACGGATATTTTTTACAGAATACTTTTCAGTACAAGCGACTGTATTACGTTCTGTCatatctgg                                                                                                                                                     <  2:323347/251‑5 (MQ=17)                                                                                 cagtcaggTTAACGGCAGCAAAGAACCTAGCAGAGGGGGTAGAGCATTATAACGAATGGCATCCGCATGGTGCGCTGGGATATCACTCGCCACGCGCATATCTGCGGCAGCGGGCTTGTAATGGGATAAGTGATAACTGAAGTCTGGCAGTCTAGGGGCTCATCCACCTTGTGCTGATATGGATCCGGCTATTTGATACAGAATACTTTTCAGTACACGCGACTGTATTACAGTCTGCCATATATGATTAc                                                                                                                                                 >  2:119195/7‑251 (MQ=255)                                                                                        gTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGAt                                                                                                                                          >  2:101844/1‑251 (MQ=255)                                                                                           aaCGGCAGCAACGAACCTTGCAGGGGCGTTCGAGCATTATAACGACTGGCATACGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTGATGGGGTAAGTGATAACAGATGTCTGGAAATATCGGGGCAAATCCACCTTGTGATGATAAGCATACGGATATTTtata                                                                                                                                                                                                      >  2:45228/1‑188 (MQ=14)                                                                                           aaCGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTtata                                                                                                                                                                                                      <  1:45228/188‑1 (MQ=255)                                                                                             cGGCAGCAAAGATCCTTGTAGAGGAGTTTGAGCATTATAACGAATGGCATCCTCATAGTGCGCTGGGTTATCGCTCGCAACGGGAATATCTGTGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTGTTCAGTACAAGCGACTGTATTACAGTATGCCATATATGATTCCCTGTGATCAGga                                                                                                                                     <  1:384509/251‑1 (MQ=21)                                                                                                   caAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTACTGGGCTAAGTGATAACAGATGTCTGGCAATATAGGGGCAAATCCACCTTGTGATGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGTAGAAt                                                                                                                                >  2:237520/1‑250 (MQ=255)                                                                                                    aaaGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGCGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATa                                                                                                                               >  1:207177/1‑250 (MQ=255)                                                                                                        aaCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTgg                                                                                                                          >  1:163673/1‑251 (MQ=255)                                                                                                                 gagGCGTTCGAGCATTATAACTAATGACATCCGCATAGTGCGCTGGGTTAGCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTGCCTGTGATCAGGAAGAATAAGTGGCTGAGGttt                                                                                                                 <  2:387279/251‑1 (MQ=255)                                                                                                                   ggCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGTGTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCa                                                                                                               <  2:183090/251‑1 (MQ=255)                                                                                                                          gAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGtt                                                                                                        >  2:18819/1‑250 (MQ=255)                                                                                                                                 ataACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATTCTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCgg                                                                                                  <  1:490285/250‑1 (MQ=255)                                                                                                                                    aCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGtata                                                                                              <  1:193694/251‑1 (MQ=255)                                                                                                                                                  cATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCtt                                                                                >  1:26966/1‑251 (MQ=255)                                                                                                                                                   aTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTa                                                                               <  1:131925/251‑1 (MQ=255)                                                                                                                                                      gTGCGCTGGGGTATCGCTCGCCACGGGAATTTCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGTGGCAAATCCACCTTGTGCTGATATGAATACTGATATTTTATACAGAATACTTTTCAGTACAAGCGACTTTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCGGAGGTTTCAAACTGTCTGCCTGTATATTCCTCTCTCCCTTaaaa                                                                            <  2:49736/251‑1 (MQ=38)                                                                                                                                                          gcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAAcc                                                                         >  1:170670/1‑250 (MQ=255)                                                                                                                                                          gcTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCa                                                                        <  2:492584/251‑1 (MQ=255)                                                                                                                                                                  aTCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAgg                                                                 <  1:76124/250‑1 (MQ=255)                                                                                                                                                                    cgctcgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTCAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgtt                                                               >  2:495920/1‑250 (MQ=255)                                                                                                                                                                        cgcCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGgttagtt                                                           <  2:426454/250‑1 (MQ=255)                                                                                                                                                                         gcCACGGGAATATCTGCGGCAGCGGGCTTGTACTGGGTTAAGTGATAACAGATGTCTGGAACTATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGACTGCCATATATGATTACCTGTGATAAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACAATAACAGGTTAGTTAt                                                         >  2:274142/1‑251 (MQ=255)                                                                                                                                                                                  atatCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTg                                                <  2:424991/251‑1 (MQ=255)                                                                                                                                                                                          ggCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCgg                                         >  2:159410/1‑250 (MQ=255)                                                                                                                                                                                                  gCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCGGAGGTTTCAATCTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATgg                                >  2:193998/1‑251 (MQ=255)                                                                                                                                                                                                             gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTg                                                                                                                                                                           >  2:245303/1‑101 (MQ=255)                                                                                                                                                                                                             gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTg                                                                                                                                                                           <  1:245303/101‑1 (MQ=255)                                                                                                                                                                                                                   tGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTAc               >  2:501160/1‑251 (MQ=255)                                                                                                                                                                                                                               tCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTc    >  1:401271/1‑250 (MQ=255)                                                                                                                                                                                                                                cTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCATTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCAt  >  1:398987/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                     CGGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCA‑GACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCAT  >  NC_000913/4499267‑4499739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.