Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,344,081 IS2 (–) +5 bp coding (713‑717/1422 nt) melB → melibiose:H(+)/Na(+)/Li(+) symporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 3187426NA (NA)62 (1.100) 51/492 0.2 100% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 = 4344085 0 (0.000)coding (717/1422 nt) melB melibiose:H(+)/Na(+)/Li(+) symporter
* ? NC_000913 4344081 =0 (0.000)65 (1.160) 55/492 0.2 100% coding (713/1422 nt) melB melibiose:H(+)/Na(+)/Li(+) symporter
?NC_000913 4498181 = NA (NA)noncoding (1/1331 nt) IS2 repeat region

AAACAGTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGT  >  NC_000913/4497932‑4498421
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                
aaaCAGTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCCATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGt                                                                                                                                                                                                                                                  >  1:295997/1‑250 (MQ=255)
     gTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTg                                                                                                                                                                                                                                            >  1:358034/1‑251 (MQ=255)
       aaTCATCCCGGGCAAATGCCCGGGAATTATTCTCGGATTATTTTCTTAGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTCTTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGAACTCACAGGGTGATGCGGAATAACTGACGTAAGGAGTGCTGCAGGGCATTGATGGTCCCTTGTAGACTGGc                                                                                                                                                                                                                                          >  1:523787/1‑251 (MQ=255)
        atcatcCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGAATTAACTGATGTATTTACTATTTACCGATCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGcc                                                                                                                                                                                                                                         >  1:79890/1‑251 (MQ=255)
             cccGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCACTGCAGGGGGGCTTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGCCGTAAGGAGTGCTGCAGGGATTTGTTGGTCCCTTGTAGACAGGCCCCCTg                                                                                                                                                                                                                                    >  2:470177/1‑251 (MQ=255)
                                  tattatAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACAGCTACCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACCTTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGTGATTTGATGGTCCCTTGAAGACTGGACCCCTGAATCTCCAGACAACCAATATc                                                                                                                                                                                                               >  2:48128/1‑251 (MQ=255)
                                   attCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATc                                                                                                                                                                                                               >  1:315305/1‑250 (MQ=255)
                                           attattTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCCGACAACCAATATCACTTAAAt                                                                                                                                                                                                       >  1:539771/1‑250 (MQ=255)
                                              attTTCGTTGTTAAAACAGACAAACGGTATTAACTGATGTATTTACTGTTTACCGCACCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAgg                                                                                                                                                                                                   >  2:185718/1‑250 (MQ=255)
                                                           aaaaaGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAACAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAAt                                                                                                                                                                                       >  1:514365/1‑250 (MQ=255)
                                                             caaGACAACCGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAACAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTAATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCATGAATCTCCAGACAACCCATATCACTTAAATAAGTGATAGTCTTAATACt                                                                                                                                                                                    >  2:344120/2‑251 (MQ=255)
                                                                            tAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCACTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGt                                                                                                                                                                     >  2:467705/1‑251 (MQ=255)
                                                                                                    gCTCCCTGCAGGGGGGATTATCAGAATTCGCGGTTATTCATGATTTATATACCTTCAACAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATCACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGCGATAGTCTTAATACTAGTTTTTATACTAGTCATTGGAGAACAGATGATTGCTGt                                                                                                                                             >  1:12574/1‑251 (MQ=37)
                                                                                                                      tATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGAAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCACCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGTGCCGGAGAAACg                                                                                                                           >  2:396094/1‑251 (MQ=255)
                                                                                                                       aTCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGACACCTCATAAACAATCCTGTACTCACAGGGTAATGCGGGATAACTCACGAAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCCCCCGACACCCAATATAACTTCAATAAGAGATAGAATTAATACTAGCTTTTAACCTAGTCATTGGAGAACAGATGATTGCTGTCTTAGGGCCGGGGACACGc                                                                                                                          >  1:38455/1‑251 (MQ=255)
                                                                                                                              tCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACggg                                                                                                                   >  1:7544/1‑250 (MQ=255)
                                                                                                                                   ggTTATACATTCTCTATATACATCCGATAAAAAACAAAAATCCTAAAAAATACTGTACTCACAGGGAGATGAGGGTTAAATGACGTAAGGCGTGCTGCCGGGACTTGATGGTCCATTGCAGACTGGCACAATGAGTCTCCAGACACCAAATATCACTTAAATAAGTGATCGTCTTAATCATAGATCTTAGACTAGTACTTGTAGAACAGCTGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGAGTAcc                                                                                                              >  2:505780/1‑251 (MQ=9)
                                                                                                                                      tATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACcac                                                                                                            >  1:378370/1‑250 (MQ=255)
                                                                                                                                                       ccttCAATAAAAAAGTAACCTCAGAAAAAATCCTGTACTCCCATGGTTATGCGGGATAACTTACGTAAGGAGTGCTTCATGGATTTGATGGGCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAAGATTGATGTATGAGGGCCGTAGAAACGCAGACGGCGTACCACACAGCAAAAGATCGCAAt                                                                                          <  2:118033/249‑1 (MQ=18)
                                                                                                                                                        aTTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAAtt                                                                                         >  2:512075/1‑251 (MQ=255)
                                                                                                                                                               aaaaaaGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATTGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGGCATAGGGCCGGAGAAACTCATTCGGCGTACCACACAGGAAAAGAGCGCAATTGTTcag                                                                                   <  2:108889/250‑1 (MQ=21)
                                                                                                                                                                aaaaaGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGAGAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATTATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTcagca                                                                                 <  1:495548/251‑1 (MQ=255)
                                                                                                                                                                 caaaGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCACTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGGAACGCAGACGGCGTCCCACACAGGAAAAGATAGCAGTTGTTcagccg                                                                                >  2:452352/2‑249 (MQ=255)
                                                                                                                                                                       aaCCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCttt                                                                          >  1:264502/1‑251 (MQ=255)
                                                                                                                                                                           tCATAAAAAATCCTGTACTAAAAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCACTTGTAGACTGGCAACCTGAATCTCAAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGCACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTCCCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAAc                                                                      >  2:530014/1‑251 (MQ=18)
                                                                                                                                                                           tCATAAAAAATACTGTACTCAAAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGCTTGATGTCTTAGGGCAGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGAAATTGTTCAGCAGAGCTTTGAAc                                                                      >  2:487430/1‑251 (MQ=255)
                                                                                                                                                                            cATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAAcc                                                                     <  2:358034/251‑1 (MQ=255)
                                                                                                                                                                             aTAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCg                                                                    >  1:545553/1‑251 (MQ=255)
                                                                                                                                                                              tAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCgg                                                                   >  2:262799/1‑251 (MQ=255)
                                                                                                                                                                                  aaaTCCTGTACTAACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCACCCTGAATCTCCAGACAACCAATATCACTTCAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTATTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCggggct                                                               >  2:26656/1‑249 (MQ=255)
                                                                                                                                                                                               acaGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTc                                                  <  2:106081/251‑1 (MQ=255)
                                                                                                                                                                                                 aGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGt                                                >  2:177353/1‑251 (MQ=255)
                                                                                                                                                                                                      gATGCGGGATAACGGACGTAAGGAGTGCTGCAGGGATTTGATTGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGccc                                           <  1:304516/251‑1 (MQ=255)
                                                                                                                                                                                                                aaCTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATgg                                 >  1:238618/1‑251 (MQ=255)
                                                                                                                                                                                                                      cGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTagc                           >  2:441530/1‑251 (MQ=255)
                                                                                                                                                                                                                       gTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTagc                           >  1:545303/1‑250 (MQ=255)
                                                                                                                                                                                                                        tAAGGTGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATCCTAGTTTTTAGACTCGTCATTGGAGAACAGATGATTGATGTCTTAGGGACGGAGAAACGCAGAAGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGTGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCGGGCAAACTGGTGTagc                           >  2:259949/1‑249 (MQ=14)
                                                                                                                                                                                                                         aaGGAGTGCTGCAGGGATTTGAGGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTagcag                         <  2:80557/250‑1 (MQ=255)
                                                                                                                                                                                                                                gctgcAGGGATTTGATGATACCTTGTAGACTCGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTAAATACTAGTTTTTAGACTAGTCATTGGAGCACAGATGATTGATGTCTTAGGGCCGGAAAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCACTCGTTGCCCGGCAACATGTGGTAGCagccagcc                   >  1:202315/1‑249 (MQ=11)
                                                                                                                                                                                                                                  tgcAGGGATTTGATGGTCACTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAACAGATCGCACTTGTTCAGCAGAGCTTTGAACAGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGCGTAGCAGCCAGCCAGtt               >  2:375846/1‑251 (MQ=255)
                                                                                                                                                                                                                                    cAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTAt             >  1:494535/1‑251 (MQ=39)
                                                                                                                                                                                                                                      gggATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTGCAGCAGAGCTTTGAACCTGGGATGACGGTCTCCCTCGTTGCCCGGCAACATCGTGTAGCAGCCAGCCAGGTAttt           <  2:514365/251‑1 (MQ=255)
                                                                                                                                                                                                                                       ggATTTGATGGTCCCTTGAAGACTGGCCCCCTGAATCTCCAGACAACCACTCACACTTAAATAAGCGACAGTCTTAATACCAGCTTTACAACTAGTCATTGGAGCACAGATGATTGTAGTCTTACGGCCGGAGCAACCCAGACGGCGTACCCCACAGGAAAAGATCGCAATTGTTCAGCAGCGCTTTGAACCGCGGCTGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTtc          >  1:331604/1‑251 (MQ=11)
                                                                                                                                                                                                                                             gATGGTGCCTTGTAGACTGTCCACCTGAATCTCCAGACCACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGCAACGCAGACGGCGTACCACACAGGAAAAGATCGCACTTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGc    >  2:50261/1‑251 (MQ=255)
                                                                                                                                                                                                                                               tGGTCCCTTGTAGACTGGCCCCCTGCATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGCTGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGt  >  2:411529/1‑251 (MQ=18)
                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                
AAACAGTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGT  >  NC_000913/4497932‑4498421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.