Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,640,418	IS30 (–) +2 bp	coding (17‑18/141 nt)	yjjY →	uncharacterized protein YjjY

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4507459 =	NA (NA)	49 (0.880)	46/488	0.3	100%	noncoding (1221/1221 nt)	IS30	repeat region
	?	NC_000913	= 4640419	0 (0.000)					coding (18/141 nt)	yjjY	uncharacterized protein YjjY
*	?	NC_000913	= 4508679	NA (NA)	42 (0.750)	39/490	0.4	100%	noncoding (1/1221 nt)	IS30	repeat region
	?	NC_000913	4640418 =	0 (0.000)					coding (17/141 nt)	yjjY	uncharacterized protein YjjY

AGGTGAGCTACAGCCCGCTTACGCTCATGGGGTTTTATGCCGCCAGTATCCCTTAACATAGTGAAGATCGTTCCGGGTTTTGAACCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGG  >  NC_000913/4508442‑4508929                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                           aggggAGCTACAGCCCGCTTACGCTCATGGGGTTTTATGCCGCCAGTATCCCTTAACATAGTGAAGATCGTTCCGGGTTTTGAACCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAATTGTTCTTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCAAATAACTATTGCGTGGACCAATTGACTCTACAGTTGCttttttt                                                                                                                                                                                                                                                >  2:259901/5‑250 (MQ=11)                           aTGGGGTTTTATGCCGCCAGTATCCCTTAACATAGTGAAGATCGTTCCGGGTTTTGAACCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTc                                                                                                                                                                                                                      >  1:144303/1‑250 (MQ=255)                                gTTTTATGCCGCCAGTATCCCTTAACATAGTGAAGATCGTTCCGGGTTTTGAACCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTaa                                                                                                                                                                                                                <  2:332426/251‑1 (MQ=255)                                                aTCCCTTAACATAGTGAAGATCGTTCCGGGTTTTGAACCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGc                                                                                                                                                                                                <  1:321869/251‑1 (MQ=255)                                                            aGTGAAGATCGTTCCGGGTTTTGAACCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTGTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCGCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTGTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAGTTCAGa                                                                                                                                                                                     <  2:499843/250‑1 (MQ=255)                                                                                ttGAACCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGAGTCCAGTTCAGCCACCGTGc                                                                                                                                                                 <  2:278539/250‑1 (MQ=255)                                                                                  gAACCCAGGATATTCGCTATTTCACAGAAGCCTGTTCCGTTCTTCCGTAGTCCAACAGCAGTGGCTTTTTCCTCTGCTGCACATGTTCGTCTCAATCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGCCTCTACAGTTGCTTTTTTTACTATATCTAGCTCAAGGCGCACTTAATTTAATGCCTTACGGAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCggg                                                                                                                                                              >  2:224728/1‑251 (MQ=18)                                                                                    aCCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGaa                                                                                                                                                            <  1:372950/251‑1 (MQ=255)                                                                                     cccAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAAc                                                                                                                                                           >  2:466693/1‑251 (MQ=255)                                                                                       cAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGCATTTCAGATTCCAGTTCAGCCACCGTGCGGGAAcc                                                                                                                                                          >  2:12968/1‑250 (MQ=255)                                                                                        aGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAAcc                                                                                                                                                          >  2:158124/1‑249 (MQ=255)                                                                                        aGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAg                                                                                                                                                        <  2:319666/251‑1 (MQ=255)                                                                                            tatTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAttt                                                                                                                                                                                         >  1:15339/1‑214 (MQ=255)                                                                                            tatTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAAttt                                                                                                                                                                                         <  2:15339/214‑1 (MQ=255)                                                                                               tCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTACATGTTCGTCTCATTCAAAAAACCTCCGCAAACCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGtccc                                                                                                                                                 >  1:337558/1‑248 (MQ=255)                                                                                                        cccTGAAGCCTGTTCCGGTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTTCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAAATGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGatctcttt                                                                                                                                        <  2:299633/249‑8 (MQ=255)                                                                                                        cACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCttt                                                                                                                                        <  1:439854/251‑1 (MQ=255)                                                                                                        cACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCtt                                                                                                                                         <  2:256683/250‑1 (MQ=255)                                                                                                                ccatttCCGTTCTTCCATAGTTCAAAAACAGATTCTTTTTCCTCTTCTGTAAATGTTCGTCTCATTCAAAAAACCACCTCAACCCCATGTTTTCACATAACTGTTTCGTTGACCAATTGTATCGACAGTTGCTTTTTGTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGACTCCAGTGCAGCCTCCGTGCGCGATCCAGGAGTACCAAGCCCTTTTCcggcgg                                                                                                                                <  2:293795/247‑1 (MQ=21)                                                                                                                      ccGTGCTTCCATAGTTCAAAAACAGTGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGAAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAGCCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTaa                                                                                                                          <  2:427013/251‑1 (MQ=255)                                                                                                                             tcCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCAt                                                                                                                     <  2:168161/249‑1 (MQ=255)                                                                                                                                  aGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTGAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCt                                                                                                               <  1:330927/250‑1 (MQ=255)                                                                                                                                        aaaaCAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGc                                                                                                        <  2:276659/251‑1 (MQ=255)                                                                                                                                                       tCCTCTGCTGTAAATGTTCGTCTCATTCAAACAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGACTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGACGGTAACCCATTGTCCTAAAGTGCCTTCCGGAAGTGAt                                                                                          >  2:42150/1‑250 (MQ=255)                                                                                                                                                             gctgTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCgg                                                                                    <  1:125587/250‑1 (MQ=255)                                                                                                                                                                gTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATATTCGGGa                                                                                  >  1:507584/1‑249 (MQ=255)                                                                                                                                                                 tAAATGTTCGTATCATTCAAAACACCTCCGCCACCCCATGTTTTCCCATAACTGTTGCGTTAACCAATTGAATCTACAATTGCTTTTTTAAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGCTCAGCCACCGTGCGGGAACAAGGAGTACCGAGACCTTTTCTGGCGGCGGTATCCAATTGACCTAAAGCGCCTACAGGAAGAGATCAACGGGAAgc                                                                               >  2:367944/1‑251 (MQ=255)                                                                                                                                                                       ttCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTc                                                                         >  1:200265/1‑251 (MQ=255)                                                                                                                                                                           cctcAATCAAAAAACCTCCGCAACCCCATGTTTGCACATAACTGGTGCGTTGTCCAATTGAATCTTCAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCGTACGCAGTTGCATAATGTCAGATTCCCTTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCAGTGTCCTAGAGTGCCTTCAGGAAGAGGTAATCGGGAAGCGCCTTCACTg                                                                     <  2:274112/250‑1 (MQ=255)                                                                                                                                                                                  cAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGaa                                                               <  1:106686/250‑1 (MQ=255)                                                                                                                                                                                  cAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATAGCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAATTGCCTTCAGGAAGAGATAAGCGGGAAGCGCCTTCACTTATCGaaa                                                              <  2:510745/251‑1 (MQ=255)                                                                                                                                                                                            ccGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGAtttt                                                     <  2:538608/250‑1 (MQ=255)                                                                                                                                                                                                aaCCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCa                                                   >  1:416695/1‑248 (MQ=255)                                                                                                                                                                                                     cATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGt                                           >  1:86417/1‑251 (MQ=255)                                                                                                                                                                                                      aTGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGtt                                          >  2:457952/1‑251 (MQ=255)                                                                                                                                                                                                         ttttCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTATTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTAAGCAGTTGCAGAATTTCAGATTCCAGTTCAGCAACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGACTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTg                                       >  1:47643/1‑251 (MQ=255)                                                                                                                                                                                                          tttCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAAGCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTg                                       <  2:399623/250‑1 (MQ=255)                                                                                                                                                                                                                   aCTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCgg                             <  1:25728/251‑1 (MQ=255)                                                                                                                                                                                                                        tGCGTTGAACAATTGAATCAACCATTGCCTTTTTTAATCTATCTCGCTCAAGGAGAACTTCATTAAACGCCTTACGCAGTTGCAGTATTTAAGATTCCTGTTCAGCCACCGTGCGGGACCCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTCCCCCATTGTCCTAAAGAGACTTCAGGCAGCGATACTCGGGAAGCGCCTCCACTGTCCGCAAGTTGATTTTCAAAAACCGTTCTGACAGCTTCGGCTTTg                        >  2:354820/1‑251 (MQ=255)                                                                                                                                                                                                                              gACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATGTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAATTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTTAAGAACCGTTCTGACAGCTGCGGCTTTGAACtt                   <  1:449101/250‑1 (MQ=255)                                                                                                                                                                                                                                    ttGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCCTTTAACGCCTTACGCAGTTGCAGAGTTACCGATTCCAGTTCAGCCACCGTGAGGGAACCAGGAGTACCGCGCCATTTTCTGGCGGCGGTAACACATTGTCCTAAAATGCCTTCAGGCAGAGATAATCGGGAAGCGCCTTGACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTag              >  1:229079/1‑249 (MQ=255)                                                                                                                                                                                                                                    ttGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTag              >  1:142303/1‑249 (MQ=255)                                                                                                                                                                                                                                              aGTTGATTTATTTACTATATCTCGCTCAAGACGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGCGTACCGAGCCCTTTTATGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTggg  >  2:522044/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                           AGGTGAGCTACAGCCCGCTTACGCTCATGGGGTTTTATGCCGCCAGTATCCCTTAACATAGTGAAGATCGTTCCGGGTTTTGAACCCAGGATATTCGCTATTTCACTGAAGCCTGTTCCGTTCTTCCATAGTTCAAAAACAGAGGCTTTTTCCTCTGCTGTAAATGTTCGTCTCATTCAAAAAACCTCCGCAACCCCATGTTTTCACATAACTGTTGCGTTGACCAATTGAATCTACAGTTGCTTTTTTTAATATATCTCGCTCAAGGCGAGCTTCATTTAACGCCTTACGCAGTTGCAGAATTTCAGATTCCAGTTCAGCCACCGTGCGGGAACCAGGAGTACCGAGCCCTTTTCTGGCGGCGGTAACCCATTGTCCTAAAGTGCCTTCAGGAAGAGATAATCGGGAAGCGCCTTCACTGATCGAAAGTTGATTTTCAAGAACCGTTCTGACAGCTTCGGCTTTGAACTTTTTAGAGTAACGTTGGG  >  NC_000913/4508442‑4508929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.