| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,173,363 | 0 | C | . | 92.3% | 99.2 / 2.0 | 26 | intergenic (‑490/+919) | gatD/gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base . (13/11); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01 | |||||||||||
| * | NC_000913 | 2,173,364 | 0 | C | . | 100.0% | 115.5 / NA | 26 | intergenic (‑491/+918) | gatD/gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (13/13); total (13/13) | |||||||||||
AGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGAT‑CC‑ACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCC > NC_000913/2173122‑2173598 || aGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGAT‑CC‑ACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTc > 1:532082/1‑251 (MQ=255) cggtggggtttgaaaaCAAATAGTTTGCGTCGCTAT‑CC‑CCAGGGTGATGCTCATAAGGATGACCCCCGAGATTAAGGTTCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCCCGAAGAAGCCGATGGTGGCAAGATCGCCAAATGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGTATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACg < 2:416182/238‑1 (MQ=255) gCAAACCAATAGTTTGCGTCGCGAT‑CC‑ACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAgg > 1:32912/1‑251 (MQ=255) aGTTTGCGTCGCGAT‑CC‑ACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg > 1:70473/1‑250 (MQ=255) gTTTGCGTCGCGAT‑CC‑ACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGa > 1:481399/1‑250 (MQ=255) gTTTGCGTCGC‑AT‑CCCACAGGTTGATTCTCAGAATAATGACACCCGAGATTAAGTTGCGAAAAAGATTTCCACGATGCCCGGCGACCGCCACCGCCACGAAGCAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGGCGGATCAAGGCCAATCAGGaa < 2:129960/251‑1 (MQ=255) gtccgcgAT‑CC‑ACAGGTTGATGTCCATAATGATGCCACCCGAAATTAGGGTGCGGACCAAATTTCCACACGGCGCGTCACCCGCCATCGCAACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCCCCTGATTACCCGGCACACAAACAGCAAGTAAAATGGTGGGTGGGATAAAAATCAGGCTTGCCGAT‑CCAACC‑GCCGTTTGTCCCAGCAGCAACGCCGGATCAAGGCCAACCAGGAACTCCt < 2:402248/248‑1 (MQ=255) gTCGCGATGCC‑A‑AGGGTGATGCTCATAATGCTTACACCCGGGATTAAGGTGCGGAACAGAGTTCCAAGATGCACGCCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACATGATTACCCGGCACACAAAAAGCAATTAAAATGGTGCGAGGGCTCAAACTCAGGCATGCCGATACCACC‑‑GCCGTATGTCCTAGCAGCAACCCCGGCACCAGGCCCATACGGAACTCCTg > 2:498772/1‑251 (MQ=255) cc‑ACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgccg > 1:205545/1‑250 (MQ=255) attgatgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCCCGGCGCCCCCCATCGCCACTAAGAAGCCGATGGTGCCAAGATCGCCAAACGGCAGCCCCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGTCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGAc < 1:132161/249‑1 (MQ=255) atgaCCCCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCCCGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGGGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg < 1:58167/251‑1 (MQ=255) gcggacaagtttcccACGATGCACGGCGACCGCCATCGCCACTAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTCCCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCATGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCg < 1:215439/238‑1 (MQ=255) cgggcaCCGCCTTCACCACGAAGACGCCGATGGTGCAAAGATCGCCAAACGGCAGCACCTGTTTACCCGGCACACAAACAGCAATTAAAAGGGTGAGGGGGAAAAAAACCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa < 2:532082/246‑1 (MQ=255) cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGGGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACt < 1:181100/251‑1 (MQ=255) cccAAACGGCAGCACCGGATTCCCCGGCACACAAACAGCAATTAAAAGGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCg < 1:641/250‑1 (MQ=255) aaCGGCAGCACATGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc > 1:464710/1‑249 (MQ=255) cccgggCACAAAAACAACAAATAAAAGGGTGAGTGGAATAAAAATCAGGCTGGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACCCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGa < 2:293029/247‑1 (MQ=255) cacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTa > 2:489088/1‑250 (MQ=255) acaAACAGCAATTAAAATGGTGAGTGGGATAAAAACCAGGCTTGCCGATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAAc < 1:483561/251‑1 (MQ=255) agaacaataaaagggggaggggaaaaaaaaaCAGGCTGCCCGATAC‑ACCC‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATCAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCGTTGACATCGTAACCcgcg < 2:185261/226‑1 (MQ=255) tGAGTGGGATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTACCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCATGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCCTGATGGGTTTAATCACCAGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATc > 1:351225/1‑251 (MQ=255) gggATAAAAATCATGCTGGCGAATCCCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGTATCAAGGCCAATCAGGAACTCCGGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGccc < 1:465767/251‑1 (MQ=255) ggATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGACGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTACCCCGCGAGGATGCCGATAATCCGcccc > 1:557914/1‑251 (MQ=255) gATAAAAATCAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGCTGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGCTGCCGATACTCAGcccc > 2:166619/1‑250 (MQ=255) aaaaaacccaggcttcccgacacccccGCCGTATGTCCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCAt < 2:205545/228‑1 (MQ=255) cAGGCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAc > 1:393952/1‑251 (MQ=255) gCTTGCCGATACCACC‑‑GCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCTTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAccc > 1:281409/1‑250 (MQ=255) || AGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGAT‑CC‑ACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCC > NC_000913/2173122‑2173598 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |