Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,415	C→T	S99L (TCG→TTG)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,415	0	C	T	100.0%	198.4 / NA	53	S99L (TCG→TTG)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/21);  total (32/21)

ACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTC  >  NC_000913/3486178‑3486660                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      acaTTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAAt                                                                                                                                                                                                                                            >  2:366012/1‑249 (MQ=255) acaTTAATAAGTACCCATCCAAGAGCCCGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGGGGCAGTGCTGATAAAAGACAAAGAGGGTAAAAAAATGATCCTCTCCTTTCTGAATCAGGGTGTTTTTATTGGCGAACTGGGCCTG‑TTGGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAAt                                                                                                                                                                                                                                            <  1:366012/249‑1 (MQ=255)       aTAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCa                                                                                                                                                                                                                                     >  1:396737/1‑250 (MQ=255)            tACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGAt                                                                                                                                                                                                                               >  1:428802/1‑251 (MQ=255)                     aaGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGCAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTaa                                                                                                                                                                                                                       >  2:8421/1‑250 (MQ=255)                      agagCAAGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAAt                                                                                                                                                                                                                                            >  2:406579/1‑228 (MQ=255)                       gagCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTAGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGCAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAAcc                                                                                                                                                                                                                    >  2:166486/1‑251 (MQ=255)                          cACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                                                                                                                                                                                                                 >  1:326594/1‑251 (MQ=255)                            agCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGCACTACTTCGTTAAA‑GGCTCTGGGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGACCTGGGCCTG‑TTTGCACAGGGCCAGGAACGGAGCGCATGGGT‑ACGTGCGCAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAATTTTCGCCAATTACTTCAGGTAAAcccaga                                                                                                                                                                                                                >  2:99834/2‑247 (MQ=255)                                   ccACCAGGGTGAAAAAGCGAAAACGCTGTACTACATCGTTAAA‑GGCTGTGTGGCAGTGCTGATAAAAGACGAAGAGGGTAAAAAAATGATCCTCTCCTATCTGAATCAGGGTGATTT‑ATTGGCGAACGGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGTT‑GCGTGCAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt                                                                                                                                                                                                       <  1:117582/250‑1 (MQ=255)                                   acACCAGGGTAAAAAAGCGAAAACGTTGTACTACATCGTTAAG‑GGCTCGGTGGCGGTGCGGATCAAAGACAAGGGGGGTAAAAAAATGACCCTCTCCTATCTGACTCAGGGTGATTTTTTTGGCGAACTGGCCGTT‑TTTGAAGAGGGCCAGAAACGTAGCGCATGGGA‑ACGTGCAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg                                                                                                                                                                                                         <  2:352373/249‑1 (MQ=255)                                    cACCAGGGTGAAAAAGCGGAAACGCTGTACTACATAGTTAAC‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAAATGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt                                                                                                                                                                                                       >  1:322465/1‑251 (MQ=255)                                     aCCAGGGAGAAAAAGCGAAAACGCTGTATTACA‑GGTTAAAAGGCTCGGTGGCAGTGCGGATCAAAGACAAAGGGGGAAAAAAAATGATCCTCTCCTATCTGAATCGGGGTGATTTTATGGGCGAACGGGGCTTT‑TTGGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGGGAAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg                                                                                                                                                                                                      <  2:384208/251‑1 (MQ=255)                                          ggggAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCGGTGGCAGTGCTGATCAAAGACGAAGAGGGAAAAGAAATGTTCCTCTCCTATCTGAAGCGGGGTGATTTTATTGGCGAACGGGGCTTG‑TTTGAAGGGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt                                                                                                                                                                                                 <  2:335766/248‑1 (MQ=255)                                               aaaaGCGGAACCGCTGTACTACATCTTTAAA‑GGCGTTGTGGCAGTGCTGATCAAAGACGAAGAGGTAAAAGAAATGATCCTCTCCTATCTGAATCAGGGTTATTTTATGGGCGAACGGGGCCTG‑TTGGAAGGGGGCGGGGAACGAAGCGCAGGTGT‑ACGTGCAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTg                                                                                                                                                                                            <  1:203662/251‑1 (MQ=255)                                                        aaCGCTGTACCACATCTTTAAA‑CGCTTTGTGGCAGTGCTGAACAAAGACGAAGAGGGTAAAGAAAGCGCCCCCCCCTATCTGAACCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGAAACGTAGCGCCGGGTT‑TGGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTGTGATGCGTTTGTCTGCACAGATgg                                                                                                                                                                                   <  1:109546/251‑1 (MQ=255)                                                             tGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGcgt                                                                                                                                                                              >  2:41357/1‑251 (MQ=255)                                                                    cATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTCTTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGc                                                                                                                                                                       >  1:420655/1‑251 (MQ=255)                                                                     aTCGTTAAA‑GGCTCTGTGGCAGTGCTCATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGCCATCC‑GCCTGTGAAGTGGCTGAACTTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGAGTTTTTCTGCCCAGATGGTGCGTCGTCTGc                                                                                                                                                                       >  2:309426/1‑250 (MQ=255)                                                                         ttAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTc                                                                                                                                                                  >  1:21363/1‑251 (MQ=255)                                                                             a‑GGCTCTGGGGCGGGGCGGATCAAAGACGAAGGGGGAAAAGAAAGGATCCTCTCTTATCTGAAAAGGGGTGATTTTATTGGCGAACGGGGCCTG‑TGTGAAGAGGGCCAGGAACGTAGCGCAGGGGT‑AAGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACtt                                                                                                                                                              <  1:341010/251‑1 (MQ=255)                                                                                   ctGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCag                                                                                                                                                           >  2:348613/1‑249 (MQ=255)                                                                                     gtgGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAgaga                                                                                                                                                        >  2:284450/1‑250 (MQ=255)                                                                                          aGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTgg                                                                                                                                                  >  1:264013/1‑251 (MQ=255)                                                                                             gttGATCAAAGACGAAGAGGGAAAAGAAATGATCCCCCCCTATCTGAATCGGGGTGATTTTATGGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGAATGGGT‑ACGTGCAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCa                                                                                                                                               <  1:41357/249‑1 (MQ=255)                                                                                                 ataaaaGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACGGGGCCTG‑TTTGAGGAGGGCCGGAAACGTAGCGCATGGGT‑ACGTGGGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCt                                                                                                                                           <  1:284450/248‑1 (MQ=255)                                                                                                   cAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTgg                                                                                                                                         >  2:328944/1‑251 (MQ=255)                                                                                                          gAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCACCCTGGCGTTCCt                                                                                                                                  >  2:413196/1‑251 (MQ=255)                                                                                                           aagggggaaaagaaagGATCCTCTCCAATTGGAAGCAGGGTGATTTTATGGGGGAA‑TGGGGCTGGTTTGAAGAGGGCAAGGAACGTAGCGAATGGGT‑AGGTGCAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAGGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACGTCAGAGAAAGTGGGCAACCTGGCGTTCCTc                                                                                                                                 <  1:413196/243‑1 (MQ=255)                                                                                                              ggggTAAAAAAATGATCCTCTCCTATCTGAATGAGGGGGTTTTTATGGGCGAACTGGGCCTG‑TTTGAAGGGGGCCGGGAACGTAGCGCATGGGT‑CCGTGGAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGAc                                                                                                                              <  1:166486/250‑1 (MQ=255)                                                                                                                   aaaGAAATGCCCCTCCCTTATCTGAATCAGGTTGGTTTGATGGGAGAACGGGGCCTG‑TTGGAAGGGGGCCAGGAGGGTGGCGAAGGGGT‑ACG‑GTGAAAACCCGCCTGTGAAGTGGCTGAAATTTTTTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCGTCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGAc                                                                                                                         <  1:221689/251‑1 (MQ=255)                                                                                                                         aTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGCAGAGGGCCAGGCACGTAGCGCATGGGT‑ACGTGCGAAATCC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGgcga                                                                                                                                                                                >  1:303621/1‑189 (MQ=255)                                                                                                                                 ctcCTTTCTGAATCGGGGTAATTTTATGGGGAAACTGGGCCGG‑TTTGAAGGGGGCCAGGAACGTAGCGAAGGGGT‑ACGGGGAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGc                                                                                                             <  2:21363/249‑1 (MQ=255)                                                                                                                                 ctcCTATCTGAATCAGGGTGATTTTATTGGCGCACTGGGCCTG‑TTTGAAGAGGGACCGGCACGTAGCGCCTGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt                                                                                                                                                                         >  2:273502/1‑189 (MQ=255)                                                                                                                                 cgcctttcgaaaTCAGGGTGTTTTTATTGGCGAACGGGTCCTG‑TTTTAAGAGGGCCAGGATGGAAGCG‑AAGGGTGACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTGCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCt                                                                                                                                                                         <  1:273502/179‑1 (MQ=255)                                                                                                                                         tGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGCGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGCAATTTTGTACAACCAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGAACAGACTctg                                                                                                   >  1:442918/1‑251 (MQ=255)                                                                                                                                          aaaccgggttGATTTTATTGGCGAGCGGGGCTTG‑TTTGAAGGGGGCCAGGAACGTGGGGAAGGGGT‑AGGTGAAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTctg                                                                                                   <  1:55448/241‑1 (MQ=255)                                                                                                                                              gagggtatttttATTGGCGA‑GTGGGGCTGGTTTGAAGAGGGCCAGGAACTTGGCGAATGGGT‑AGGTGCAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGaa                                                                                              <  2:226046/243‑1 (MQ=255)                                                                                                                                              cAGGGAGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCCCAGACTCTGCTGa                                                                                               >  2:312789/1‑250 (MQ=255)                                                                                                                                                        ttATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTAAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGACTCTGGCaaa                                                                                     >  2:331477/1‑250 (MQ=255)                                                                                                                                                          aTTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAaac                                                                                   >  2:469802/1‑250 (MQ=255)                                                                                                                                                                 aaCTGGGCTTG‑TTTGAAGAGGCCCAGAAACGTAGCGCATGGGT‑ACGTGCAAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGAc                                                                           <  1:250411/251‑1 (MQ=255)                                                                                                                                                                  aatGGGCATG‑GTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGAAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACGAGACg                                                                          >  2:121884/3‑251 (MQ=255)                                                                                                                                                                                              aCGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGACAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAAACTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGAAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCaa                                                >  2:207435/1‑250 (MQ=255)                                                                                                                                                                                                               cGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTc                                >  1:114000/1‑250 (MQ=255)                                                                                                                                                                                                                    gAAAACC‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGaa                           >  2:190663/1‑250 (MQ=255)                                                                                                                                                                                                                       atcc‑GCCTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTg                       <  1:312789/249‑1 (MQ=255)                                                                                                                                                                                                                             ccTGTGAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTc                    >  1:310646/1‑249 (MQ=255)                                                                                                                                                                                                                                 tgAAGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGAAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAAACAGACGCTATGAATAACCCGGACGGTATGCAAATCAAAATTACCAGTCAGGAAATTGGTCAGATTg              >  2:307591/1‑251 (MQ=255)                                                                                                                                                                                                                                    aGTGGCTGAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCg           >  1:212402/1‑251 (MQ=255)                                                                                                                                                                                                                                        gCTGAAACTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGGGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTg       <  2:420655/251‑1 (MQ=255)                                                                                                                                                                                                                                           gAAATTTTGTACAAAAAATTTCGCCAATTGATTCAGCAACACCCGGACTTTCTGATGCGTTTGTCCGCCCACATGGCGCGTCGTCTGCACGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGACTCTGGCAAAACAACCAGACGCTATGACTCACCCGGCCGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtc    >  1:266346/1‑251 (MQ=255)                                                                                                                                                                                                                                             aaTTTTGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTtctc  <  1:331477/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      ACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAA‑GGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTG‑TTTGAAGAGGGCCAGGAACGTAGCGCATGGGT‑ACGTGCGAAAACC‑GCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCAACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAATCTGGCAAAACAACCAGACGCTATGACTCACCCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATTGGTCAGATTGTCGGCTGTTCTC  >  NC_000913/3486178‑3486660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑