Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,240,844	G→A	A301T (GCG→ACG)	sstT →	serine/threonine:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,240,844	0	G	A	100.0%	206.2 / NA	63	A301T (GCG→ACG)	sstT	serine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/35);  total (28/35)

CTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCT  >  NC_000913/3240598‑3241089                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         ctgGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATgacggg                                                                                                                                                                                                                                                      >  1:325031/1‑251 (MQ=255)   ggTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATgacgggc                                                                                                                                                                                                                                                     <  1:185939/250‑1 (MQ=255)      cGTGCTGGTTGGCTGTCTGATACTGGTGAAGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTAGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCATGAGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGCATATGGCGCTGTGTGAAAAGCTGAATCTGGATAGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCCTCAATATGACGGgcgc                                                                                                                                                                                                                                                   >  2:459625/1‑249 (MQ=255)           tGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATc                                                                                                                                                                                                                                            >  1:264392/1‑251 (MQ=255)                    gTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAGCCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCTCCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCGATTCCGCTTGGAGCCGCCATCAATATGACGGGCGCAGCAATCACTATTAc                                                                                                                                                                                                                                    <  1:422566/250‑1 (MQ=255)                    gTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTAc                                                                                                                                                                                                                                    <  2:127837/250‑1 (MQ=255)                          tACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGtt                                                                                                                                                                                                                              <  1:391042/250‑1 (MQ=255)                              ggtggCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGTATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACgc                                                                                                                                                                                                                         <  1:466427/251‑1 (MQ=255)                                 ggCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGGAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGAAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACgctgg                                                                                                                                                                                                                      >  1:341337/1‑251 (MQ=255)                                       ggtggtTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCg                                                                                                                                                                                                                 <  1:367690/250‑1 (MQ=255)                                                 ccATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACgct                                                                                                                                                                                                                        >  2:210626/1‑233 (MQ=255)                                                 ccATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACgct                                                                                                                                                                                                                        <  1:210626/233‑1 (MQ=255)                                                     ggCTGGTTTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGGATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGAGCGCGATACCTATTCCGTTTCGATTCCGCTGGGAGCCTCCATCAATATGACGTGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGt                                                                                                                                                                                                  <  1:88712/250‑1 (MQ=255)                                                       cTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTAt                                                                                                                                                                                                >  1:467230/1‑251 (MQ=255)                                                        tggtgtggtgGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTATATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTAtt                                                                                                                                                                                               >  2:325493/1‑251 (MQ=255)                                                             tggtggAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGt                                                                                                                                                                                          >  2:131380/1‑251 (MQ=255)                                                                 ggAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGa                                                                                                                                                                                       <  2:328211/250‑1 (MQ=255)                                                                             gtAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGCAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGAAGCGAACATTCCGGTGAATATGGCGCTGTGTGCAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACTCTGGCTGCGGTTAATACGCTGGGTATTTCGGTCGATCTGCCCACGgcg                                                                                                                                                                          >  2:211955/1‑251 (MQ=255)                                                                              tAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGgcgc                                                                                                                                                                         >  1:171028/1‑251 (MQ=255)                                                                               aaCCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGccc                                                                                                                                                                                <  2:37071/243‑1 (MQ=255)                                                                               aaCCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGccc                                                                                                                                                                                >  1:37071/1‑243 (MQ=255)                                                                                        ccGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAg                                                                                                                                                               <  1:318574/251‑1 (MQ=255)                                                                                             ggTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCgtg                                                                                                                                                           <  1:311690/250‑1 (MQ=255)                                                                                                gctgctGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCgtggtg                                                                                                                                                        <  1:106065/250‑1 (MQ=255)                                                                                                 gggcggtgCCTGCGCGAAAGCGTTGTGTATGCCTTCTTCACTCGCAGGTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATGACCGGGTTGACGCTGGCTGCGGTTATTACGCTGGGTATTCCGGTCGATCTGCGCACGGCTCCGCTGTTGAGCGTGGTGGc                                                                                                                                                      <  2:47896/246‑1 (MQ=255)                                                                                                        gCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCtg                                                                                                                                               <  2:352664/251‑1 (MQ=255)                                                                                                         ccTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTTGTGGCTTCTCtgt                                                                                                                                              <  2:435191/251‑1 (MQ=255)                                                                                                            gcgcGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCtgtgt                                                                                                                                            <  2:148192/250‑1 (MQ=255)                                                                                                                gAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGGGGTGGCTTCTCTGGGTGCCtg                                                                                                                                       >  2:320383/1‑251 (MQ=255)                                                                                                                        tgtgtATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCAt                                                                                                                               >  2:300759/1‑251 (MQ=255)                                                                                                                           gtATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATcc                                                                                                                             <  1:167243/250‑1 (MQ=255)                                                                                                                           gtATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATcc                                                                                                                             <  1:383617/250‑1 (MQ=255)                                                                                                                           gtATGCCTTCTTCACCAGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTGAATACGCTGGGTATTCCGGTCGATCTGCCTACGTAGCGGCTGTTGAGCGGGGTGGCTTCTCTGTGTGCCTGTGGCGCATcc                                                                                                                             <  1:199183/250‑1 (MQ=255)                                                                                                                                           cGCAGCTCTGCTGCGCACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGTGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAAGACGCTGGGTATTCCTGTCTATCTGCCCACGGCGCTGCTGTTTAGCGTGGTGGCTTCTCTGTTTGCCTGTTGCGCATCCGGCGTTGCGCGG‑GGGtc                                                                                                           <  2:166840/251‑1 (MQ=255)                                                                                                                                            gCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGACAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCAGCTGTTGAGCGTGGGGGCTTATCTGTGGGCCTGTGGCGCCTCCCGCGTGGCgggg‑ggggct                                                                                                          >  2:352553/1‑248 (MQ=255)                                                                                                                                                    gCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAATCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGT‑GGGTCTctgctgct                                                                                                  <  2:134761/251‑1 (MQ=255)                                                                                                                                                    gCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTctgctgc                                                                                                   <  1:48209/250‑1 (MQ=255)                                                                                                                                                    gCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCGATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTctgctg                                                                                                    <  2:347494/249‑1 (MQ=255)                                                                                                                                                      aGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCCTCCGGCGTGGCGGGG‑GGGTCTctggtgctgg                                                                                                >  2:286283/1‑250 (MQ=255)                                                                                                                                                      aGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTctgctgctgg                                                                                                >  1:264655/1‑250 (MQ=255)                                                                                                                                                       gCGATCATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCGTGAGCGCGATACCTATTCCGGTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTAGTTCCGTGTTGACGCTGGCTGCGGTTAAGACGCTGGGTATTCCGTTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTTTGCCTGTGGCGCAACCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAt                                                                                               <  2:242932/251‑1 (MQ=255)                                                                                                                                                          aaCATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGCTctgctgctggaccc                                                                                            >  2:249247/1‑246 (MQ=255)                                                                                                                                                          aaCATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGGGTGCCTGTGGCGCATCCGGCGTGGCGGTG‑GGGGCCCTGCTGCTGAttcc                                                                                            >  2:438839/1‑248 (MQ=255)                                                                                                                                                          aaCAGTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTTGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑ccc                                                                                           <  1:311137/251‑1 (MQ=255)                                                                                                                                                               tCCGGCGAATATGGCGCTGTGTGAAAAGCTGACTCTGGATCGCGCTACCTATTCCGTTTCTATTCCGCTGGGCGCCACCATCAATATGACGGGCGCCGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTCCTGTTGAGCGTGGGGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGTTCTTCTGCTGATTCCCACTg                                                                                       >  2:262652/1‑251 (MQ=255)                                                                                                                                                                      aaTATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGGAGCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑Taa                                                                              <  1:206506/251‑1 (MQ=255)                                                                                                                                                                       atttggtgcTGTGTCAAAATCTGAATCTGGATCGCGAGACCTACTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑Tatt                                                                             <  2:366299/244‑3 (MQ=255)                                                                                                                                                                        tatGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGAGTTGACGCTGGCTGCGGTTAATACGCTGGGGATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGCGGCGGGGCGGGAC‑CTGCTGCTGAA‑CCCACTGTCCTG‑TAat                                                                             >  1:19759/1‑250 (MQ=255)                                                                                                                                                                            gcgcTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGtt                                                                        <  2:325031/251‑1 (MQ=255)                                                                                                                                                                                tgtgtgAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAt                                                                                               <  1:141622/226‑1 (MQ=255)                                                                                                                                                                                          gCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGCTCTGCTGCTGAT‑CCCCCTGGCCTG‑TAATATTTTCgggttttttaa                                                            >  2:346768/1‑240 (MQ=255)                                                                                                                                                                                            tGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGCGCTTCTGCTTAT‑CCCACTAGCCGT‑TAATATGtttctgttttctgacaat                                                        >  2:90281/1‑235 (MQ=255)                                                                                                                                                                                                     aTCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGCTCTGCTGCTTAT‑CCCACTTGCCCG‑TAATTTGtttcggattttggaccagatcggcttg                                               >  2:280398/1‑226 (MQ=255)                                                                                                                                                                                                                    ccGTTTCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGt                                 <  2:198322/250‑1 (MQ=255)                                                                                                                                                                                                                     cGTTTCTAGTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCATTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCTTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAG‑CCCACTGGCCTG‑TAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCg                               <  2:150277/251‑1 (MQ=255)                                                                                                                                                                                                                         tCTATTCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCt                            <  1:144196/250‑1 (MQ=255)                                                                                                                                                                                                                              tCCGCTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTatc                       <  1:249247/250‑1 (MQ=255)                                                                                                                                                                                                                                  cTGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGTTCGGGATTTTGAACGGTATCGCCATGCAGGTGGTTGGCGTCGGCTTTATTATCgg                  >  1:78995/1‑251 (MQ=255)                                                                                                                                                                                                                                   tGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGCTCTGTTGCTGGT‑CCCACGGGGCTG‑TAATTAGTTCGGGAGTTCGAACGATAACCCCATGCACGGGGTTGCGGGTGGCTTTTTCATCggg                 >  2:254517/1‑250 (MQ=255)                                                                                                                                                                                                                                   tGGGAGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGCTCTGCTTGTGAT‑CACACTGGCCTG‑GAAAATTTTCGGGATTTTGAAAGATAATGCCAGGCAGGGGGGTGCCGGTGGCttttttataggg                 >  1:325874/1‑242 (MQ=255)                                                                                                                                                                                                                                       aGCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGACGGGG‑GGGGCCCCGCTGCTGGT‑CCCACTTGCCTGCTAA‑ATGTTCGGGATTTTGCACGCTATCGCCCTTCAGGGGGTTGCCGTCCGCttttttctccgcggat             >  2:38997/1‑238 (MQ=255)                                                                                                                                                                                                                                        gCCACCATCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGTTCGGTATTTCGAACGATATCGCCATg                                               <  1:436863/216‑1 (MQ=255)                                                                                                                                                                                                                                           accaTCAATATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGATGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGCTTTGCTGCTGAT‑CCCACTTGCCCG‑GATAATGTTCGGTATTTTGAAAGGTATCGCCCAGCAGGGGGGTGGCGTCGGCTTTattatccggtggttgcg         >  2:181627/1‑240 (MQ=255)                                                                                                                                                                                                                                                 aaTATGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGGCTCTGCTGCTGAT‑CCCCCTGGCCCG‑TAATATGTTCGGGATTTTGAACGATATCGCCATGCAGGGGGTTGCCGTCGGCTTTTTCATCGGCGTATTGCAGGActt   >  1:285943/1‑250 (MQ=255)                                                                                                                                                                                                                                                  atatGACGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCGCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGActct  <  2:403343/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         CTGGTCGTGCTGGTTGGCTGTATGTTACTGGTGGCGCTGGTGGTTAACCCATTGCTGGTGTGGTGGAAAATTCGTCGTAACCCGTTCCCGCTGGTGCTGCTGTGCCTGCGCGAAAGCGGTGTGTATGCCTTCTTCACCCGCAGCTCTGCAGCGAACATTCCGGTGAATATGGCGCTGTGTGAAAAGCTGAATCTGGATCGCGATACCTATTCCGTTTCTATTCCGCTGGGAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGG‑GGGTCTCTGCTGCTGAT‑CCCACTGGCCTG‑TAATATGTTCGGTATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCT  >  NC_000913/3240598‑3241089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑