Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,823,537	A→G	L78P (CTG→CCG)	recA ←	DNA recombination/repair protein RecA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,823,537	0	A	G	100.0%	202.0 / NA	64	L78P (CTG→CCG)	recA	DNA recombination/repair protein RecA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/36);  total (28/36)

GATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCT  >  NC_000913/2823290‑2823779                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   gATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATCAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTgcggcg                                                                                                                                                                                                                                                 >  1:53245/1‑251 (MQ=255) cattccGCCTTCGATTTCCGCCTTCGGCTTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTGGTCGATATCGACGCCCAGTTTCCGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTgcggcg                                                                                                                                                                                                                                                 <  2:17929/246‑1 (MQ=255)              aTTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGtttt                                                                                                                                                                                                                                    <  1:245830/251‑1 (MQ=255)                  ccGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCg                                                                                                                                                                                                                                >  1:425299/1‑251 (MQ=255)                     cTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTCCCggc                                                                                                                                                                                                                              >  2:140082/1‑249 (MQ=255)                       ttCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGa                                                                                                                                                                                                                              >  2:422598/1‑248 (MQ=255)                                   gccgccACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTAAGCGTGGTTTTACCGGAAGATTCCGGTCCGt                                                                                                                                                                                                                >  1:189685/1‑250 (MQ=255)                                       ccACGGAGTCAACGACGATCACGTCTACTGCGCCAGAACGCGCCAGGGCGTCAAAGCTTTCAAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGCCGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCAAGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGAtt                                                                                                                                                                                                           >  2:469538/1‑251 (MQ=255)                                          cGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTcg                                                                                                                                                                                                        <  1:324946/251‑1 (MQ=255)                                                cAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGG‑CGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATa                                                                                                                                                                                                  <  1:91419/250‑1 (MQ=255)                                                   cgacgaTAACGTCTACTGCGCCATAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGTTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCCCGCTGCGCTGCGGCGATCACCTGCGTCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTCGCTTTCGACGCTACgg                                                                                                                                                                                               >  2:107169/1‑251 (MQ=255)                                                       gaTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGccc                                                                                                                                                                                            >  1:252927/1‑250 (MQ=255)                                                        aTAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCAt                                                                                                                                                                                          <  1:112779/251‑1 (MQ=255)                                                          aaCGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCg                                                                                                                                                                                        >  2:470397/1‑251 (MQ=255)                                                              tCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTAAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGc                                                                                                                                                                                      >  2:256920/1‑249 (MQ=255)                                                                 aCTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGacc                                                                                                                                                                                 <  1:371049/251‑1 (MQ=255)                                                                      gcCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTg                                                                                                                                                                            <  1:453997/251‑1 (MQ=255)                                                                         aGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGccc                                                                                                                                                                         <  1:439192/251‑1 (MQ=255)                                                                           aaCGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGcccc                                                                                                                                                                        >  2:331410/1‑250 (MQ=255)                                                                           aaCGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGAGTCAGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGcccc                                                                                                                                                                        >  1:278655/1‑250 (MQ=255)                                                                              gcgcCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAgc                                                                                                                                                                    >  2:76390/1‑251 (MQ=255)                                                                              gcgcCAGGGCGTCACAGATTGCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGTCGTCAGCGTGTTTTTACCGGAAGATTCCGGTCCGTAGATCTCGACGAAACGGCCCATCGGCAGACCACCTGCCCCAAgc                                                                                                                                                                    <  1:470348/251‑1 (MQ=255)                                                                                      gCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACATCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGAGTCCGGTCCGTAGATTGCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATc                                                                                                                                                            <  2:425299/251‑1 (MQ=255)                                                                                        gTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATcc                                                                                                                                                           >  2:448285/1‑250 (MQ=255)                                                                                          cacaGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAg                                                                                                                                                         <  2:48813/250‑1 (MQ=255)                                                                                             aGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGa                                                                                                                                                      >  1:430961/1‑250 (MQ=255)                                                                                              gATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGaaa                                                                                                                                                    <  1:215978/251‑1 (MQ=255)                                                                                                  tCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGAGAAAGCg                                                                                                                                                 >  1:427615/1‑250 (MQ=255)                                                                                                    cAGTGCCTGCTCGCCGGTGTCCGTCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGGGTGCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAAc                                                                                                                                              <  2:53245/251‑1 (MQ=255)                                                                                                      gTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCTCCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCg                                                                                                                                            <  1:469584/251‑1 (MQ=255)                                                                                                            gCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTagag                                                                                                                                      <  1:331410/251‑1 (MQ=255)                                                                                                                             ggggAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCAt                                                                                                                     <  2:459569/250‑1 (MQ=255)                                                                                                                                     cagcagGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGt                                                                                                             >  1:94112/1‑251 (MQ=255)                                                                                                                                              gTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCa                                                                                                    <  1:140985/251‑1 (MQ=255)                                                                                                                                                ggATATCGACGCCCAGTTTACGTGCGTAGATTGGGGCCAGCGCGTGTTCAGCACCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTCCCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCAGCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCCCCCAgt                                                                                                  <  2:252927/250‑2 (MQ=255)                                                                                                                                                      cgccGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTCCCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCat                                                                                             <  1:324589/247‑1 (MQ=255)                                                                                                                                                             cAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTGCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAgt                                                                                     <  2:282265/251‑2 (MQ=255)                                                                                                                                                                  tACGGGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCGGCGGCGATCACCTGCGGCGTCAGCTTGGTTTTACCGGAAGATTCCGGGCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCttt                                                                                 <  2:189685/250‑1 (MQ=255)                                                                                                                                                                   aCGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTAc                                                                               <  1:15185/251‑1 (MQ=255)                                                                                                                                                                     gTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTAcc                                                                              >  1:252086/1‑250 (MQ=255)                                                                                                                                                                        acgaggctcttctgatttcggggtCAGCAGCGATAAACGCACAGGGTTTACCTTCAGGCTGCGAGGGGGCGATCACCTGCGGCGGCAGGGTGGTTTTACCGGAAGATTCCGGGCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAAt                                                                          <  2:219059/228‑1 (MQ=255)                                                                                                                                                                           aGATGGGGGCCAGCGCGTGTGCAGCATCGATCAACGCGTAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTg                                                                        <  2:182334/250‑1 (MQ=255)                                                                                                                                                                            gATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGGAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGtt                                                                      >  1:324426/1‑251 (MQ=255)                                                                                                                                                                            gATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGCTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGCAGATTCCGGTCCGTAGATTTCGCCGATACGGCCCATCGGAAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCAAGGCGCATGATGGAGCCTTTACCAAATTGtt                                                                      >  1:63259/1‑251 (MQ=255)                                                                                                                                                                             aTTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGttt                                                                     >  1:258049/1‑251 (MQ=255)                                                                                                                                                                               tGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCGCGCTGAGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTACGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGAGAAAGCGCACCGGTAGAGACGGTTTCCACCTCCATGGAACGGTCTTCCCCCCTGCGCAAGATGGAGCCATTACCAAATTGTTtct                                                                   >  2:53704/1‑251 (MQ=255)                                                                                                                                                                                gggTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTtct                                                                   <  1:94906/250‑1 (MQ=255)                                                                                                                                                                                    ccAGTGCGTGTTCAGCAGCGATAACCGCACAGGTGTTTCCGTCGCGCGGCGCTGCGGCGATCACCGGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGGCCGTAGATTTCGACGATACGGCCCATCGGCAGACCCCCTGCCCCAAGCGCGATATCCAGTGATAGCGAACCGGTAGAGATTGTTGCCGCATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTCCCAAATTGTTTCTCAAt                                                               <  2:453267/250‑1 (MQ=255)                                                                                                                                                                                    ccAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGAGCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATc                                                              <  2:114628/251‑1 (MQ=255)                                                                                                                                                                                      aGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCGTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTg                                                            <  1:422683/251‑1 (MQ=255)                                                                                                                                                                                          cgTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAAt                                                                          >  1:219059/1‑233 (MQ=255)                                                                                                                                                                                             gtTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGt                                                     >  2:158589/1‑251 (MQ=255)                                                                                                                                                                                                      cGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGAGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTgccgcc                                            >  1:35722/1‑251 (MQ=255)                                                                                                                                                                                                       gATAAACGCACAGTTTTTACCTTCACGCTGCGCTGCGGCGATCACCGGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGTCGATACGGCCCATCGGCAGACCACCTTCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTGTACCAAATTGTTTCTCAATCTGTCCCAGTGCTGCCGCCa                                           <  2:113669/251‑1 (MQ=255)                                                                                                                                                                                                                  aGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCtgt                                >  2:216999/1‑251 (MQ=255)                                                                                                                                                                                                                      tttACCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCtgtttg                             <  1:13805/250‑1 (MQ=255)                                                                                                                                                                                                                         aCCTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTgtttgttt                          <  1:350540/250‑1 (MQ=255)                                                                                                                                                                                                                          ccTTCACGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTGTACCGGAAGATTCCGGGCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACAGCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTtc                        <  2:146155/251‑1 (MQ=255)                                                                                                                                                                                                                               aCGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGa                   <  1:213164/251‑1 (MQ=255)                                                                                                                                                                                                                               aCGCTGCGCTGCGGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGa                   <  1:140082/251‑1 (MQ=255)                                                                                                                                                                                                                                    gtgcttcggctATCACCTGCGGCGTTAGCGTGGTTTTCACGGAAGATTCCGGGCCGTAGATTTCGACGATACGGCCCATCGTCAGACCCCCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGcc              <  2:351473/245‑1 (MQ=255)                                                                                                                                                                                                                                     cgctgcgGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGCTTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGGACCGGTAGAGATGGTTTCCCCATCCATGGCACGGTCTTCCCCCAGGCCCATGATGGAGCCTTTAACAAATTGTTTCTCACTCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATTGcc              >  2:369618/1‑250 (MQ=255)                                                                                                                                                                                                                                       ctgcgGCGATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGACCGGTCTTCACCCAGGCGCATGATGGAGCCTTTCCCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCAtt           >  2:207392/1‑251 (MQ=255)                                                                                                                                                                                                                                           ggcgATCACCTGCGGCGTCAGCGTGGTTTTACCGGCAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCatgatg                                                                                         >  1:236006/1‑169 (MQ=255)                                                                                                                                                                                                                                           ggcgATCACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCGGCCCCAAGCGCGATATCCAGTGAAAGCGAACCTGTAGAGATGGTTTCCACATCCATGGAGCGGTCTTCTCCCAGGCTCatgatg                                                                                         <  2:236006/169‑1 (MQ=255)                                                                                                                                                                                                                                                 cACCTGCGGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCt  <  2:252086/250‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   GATTTCGCCTTCGATTTCCGCTTTCGGCGTCAGTGCCGCCACGGAGTCAACGACGATAACGTCTACTGCGCCAGAACGCGCCAGGGCGTCACAGATTTCCAGTGCCTGCTCGCCGGTGTCCGGCTGGGAGCACAGCAGGTTGTCGATATCGACGCCCAGTTTACGTGCGTAGATTGGGTCCAGCGCGTGTTCAGCATCGATAAACGCACAGGTTTTACCTTCACGCTGCGCTGCGGCGATCACCTGCAGCGTCAGCGTGGTTTTACCGGAAGATTCCGGTCCGTAGATTTCGACGATACGGCCCATCGGCAGACCACCTGCCCCAAGCGCGATATCCAGTGAAAGCGAACCGGTAGAGATGGTTTCCACATCCATGGAACGGTCTTCACCCAGGCGCATGATGGAGCCTTTACCAAATTGTTTCTCAATCTGGCCCAGTGCTGCCGCCAACGCTTTCTGTTTGTTTTCGTCGATAGCCATTTTTACTCCT  >  NC_000913/2823290‑2823779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑