Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,322,099	C→T	P78P (CCC→CCT)	atoC →	DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,322,099	0	C	T	100.0%	87.6 / NA	25	P78P (CCC→CCT)	atoC	DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/15);  total (10/15)

TCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTGCAAAGACA  >  NC_000913/2321857‑2322340                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  tCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCt                                                                                                                                                                                                                                           >  2:326619/1‑251 (MQ=255)      cTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCtgatg                                                                                                                                                                                                                                       >  1:301183/1‑250 (MQ=255)                                    tggggATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGGTCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTTTTGAGGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCGGCCATGAGACCCGGACACCTGTTATGCGGAGGACGGCCTATGCGGAAGTGGAAACCGCCGTc                                                                                                                                                                                                         <  2:403525/247‑1 (MQ=255)                                                        ttCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGCGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGt                                                                                                                                                                                                                      >  1:273508/1‑217 (MQ=255)                                                        ttCGCCGTATGCTGAGCACCGCGTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGTGGAAGt                                                                                                                                                                                                                      <  2:273508/217‑1 (MQ=255)                                                          cGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCt                                                                                                                                                                                   <  2:334448/250‑1 (MQ=255)                                                            ccGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACg                                                                                                                                                                                                                                    >  2:416665/1‑199 (MQ=255)                                                            ccGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACg                                                                                                                                                                                                                                    <  1:416665/199‑1 (MQ=255)                                                            ccGTATGCTGAGCACCGCTTTTGCACTACAAGGATACGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTCCCGATATTCACCCTGATGTGGTGTTGATGTATATCCGCATGCCAGAGATGGACGGCAGCAAGGCACTAAAGGGGATGCGCTGCCATGAGACCCGGATACCTGTTATTCGTATGGCGGCCTATGCGGAAGTTGAATCCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCg                                                                                                                                                                                <  1:67488/251‑1 (MQ=255)                                                                  gCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTTATGGATAGCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGGTATTCTGATGACGGCCTATGCGGAAGTGTAAACCGCCGTCGAAGCGCGACGCTGCGGAGCCTTCGACTAt                                                                                                                                                                           <  2:301183/250‑1 (MQ=255)                                                                          ccGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCGGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGTGGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTaaa                                                                                                                                                                  <  2:304572/251‑1 (MQ=255)                                                                            gCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAAcc                                                                                                                                                                <  1:103665/251‑1 (MQ=255)                                                                                                                      ggACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCttt                                                                                                                      >  2:119344/1‑251 (MQ=255)                                                                                                                                cATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAg                                                                                                            <  1:111179/251‑1 (MQ=255)                                                                                                                                    acacCTGTTTGCCGATATTCACCCGGATGTGGTGTTGATGGATAGCTGCATGCCAGAGATGGACGGAATCAAGGCACTAAAGGGGATGCGCAGCCATGAGACCCGGACACATGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCaa                                                                                                        <  2:250794/251‑1 (MQ=255)                                                                                                                                                   tatTCACCCTGATGTGGAGTTGCTGGATATCAGCATGCCCGAGATGGACGGCATCAAGGCACTCAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAACCCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATcc                                                                                         >  2:68841/1‑251 (MQ=255)                                                                                                                                                                gtggtgTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGtt                                                                                                                                                             >  2:292896/1‑170 (MQ=255)                                                                                                                                                                gtggtgTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGtt                                                                                                                                                             <  1:292896/170‑1 (MQ=255)                                                                                                                                                                              tatCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCAATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCAcc                                                               >  1:208962/1‑250 (MQ=255)                                                                                                                                                                                           gagaTGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATgggg                                                 <  1:81218/251‑1 (MQ=255)                                                                                                                                                                                                              cacgaaaGGAGATGCGCAGCCAGGAGACCCGGACACCTGTTATTCTGATGACGGCCTAGGCGGAAGTTGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGc                              <  2:33030/247‑1 (MQ=255)                                                                                                                                                                                                                  aaaGGAGATGCGCAGCCATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGccc                            <  1:282393/249‑1 (MQ=255)                                                                                                                                                                                                                                gCCATGAAACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATc            >  1:408965/1‑251 (MQ=255)                                                                                                                                                                                                                                  cATGAGACCCGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTg          >  2:243743/1‑251 (MQ=255)                                                                                                                                                                                                                                          ccGGACACCTGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCTCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTGCAAAGaca  <  1:143482/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  TCAGACTGTATGACTGCTATTAATCGCATCCTTATTGTGGATGATGAAGATAATGTTCGCCGTATGCTGAGCACCGCTTTTGCACTACAAGGATTCGAAACACATTGTGCGAACAACGGACGCACAGCATTACACCTGTTTGCCGATATTCACCCTGATGTGGTGTTGATGGATATCCGCATGCCAGAGATGGACGGCATCAAGGCACTAAAGGAGATGCGCAGCCATGAGACCCGGACACCCGTTATTCTGATGACGGCCTATGCGGAAGTGGAAACCGCCGTCGAAGCGCTACGCTGCGGAGCCTTCGACTATGTTATTAAACCGTTTGATCTCGATGAGTTGAATTTAATCGTTCAGCGCGCTTTACAACTCCAGTCAATGAAAAAAGAGATCCGTCATCTGCACCAGGCACTGAGCACCAGCTGGCAATGGGGGCACATTCTCACCAACAGCCCGGCGATGATGGACATCTGCAAAGACA  >  NC_000913/2321857‑2322340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑