Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,358,859	T→C	Y110C (TAT→TGT)	puuP ←	putrescine:H(+) symporter PuuP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,358,859	0	T	C	92.3%	43.6 / NA	26	Y110C (TAT→TGT)	puuP	putrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (14/10);  minor base A (2/0);  total (16/10)
Fisher's exact test for biased strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATC  >  NC_000913/1358627‑1359101                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    gCCTTCTCCTTTATGCAGTCCCTGCACTACCAGGAAGCTAAACAACACCATGATGGAGATTTGCACCAGTACAAACAGGGAATTGCAGATAGCGACCAGGTTGACGCTCTTAAGATTCGCGGCGGTTCAAATGGCAACGAAGGGTACCACCCACACCCACGGCGGCACCTCCGCGAAGAGGGCGGAGAGATAGATTATCGCCAACAAGAAGTTAAACAAAGGCACAAAGAGACAATCCAACAGCGATggcc                                                                                                                                                                                                                                   >  1:30114/1‑248 (MQ=255)                cAGTCCCTGCACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACCAAGGTTATCACCCACAAACACGGCGGCCATTTCGGGCAGAGGGGGGAGGGATAGATTTTCGACCACACGACCTTAAGCATAGGCAAAAAAAGCAAATACAGCAACGAAGACCAACCGACCAAAAATc                                                                                                                                                                                                                    >  2:394607/1‑251 (MQ=255)                             aCCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGctgg                                                                                                                                                                                                         <  1:48190/249‑4 (MQ=255)                              ccAGGAAGCTAAACACCACCATGATGGAGCTTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGGTACAACCCACACCCACGGCGGCACTTCCGGGAAGGGGGGGGGGAGATAGATTTTCGCACACCAGGCGGTAATCATCGGCAAAAAGAAACAAACCAGCAGCGATGACCAGCCAAccctacaacccccgtggtggtt                                                                                                                                                                                                       >  2:319542/1‑230 (MQ=255)                                aGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAAAAGGGTATTGACGTTAGCGACCAGGTTGACGATCTTCAGATTCGCGGCGGTTAAAAAGGCGACCAAGGTTACCACCACCACCCACGGCCGCACATCCCGGAAGAGGGCGGGGAGATAGAATTTCGCCAACAAACCGTTAAACATCGGCAAAAAGAAAAAAACCCGCAGCGGTGACCAACCggacaaaaaaccgacctgctggttta                                                                                                                                                                                                     >  2:394913/1‑225 (MQ=255)                                                   tgatgGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCCGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAAAGGCAACGAAGGTTATCAACCACAACCACGGCGGCACTTCCGGGAAGAGGGCGGGGAGATAGAATTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCCGCCGACCATAAATCCGACGGGCTGGTTAATCCACTTTTGCGGGTAggg                                                                                                                                                                                  >  2:258083/1‑249 (MQ=255)                                                     atgGAGATATGCTCCAGTACAAACAGGGTATTGAAGTTAGCGCCCAGGTTGACGCTCTTTAGAGTCGCGGCGGTTAAAATGGCGGCGAAGGTTACGTCCCACCGCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGTCAACAAGACTTTAATCATCGGAAAAAAGAGTCAAGCCAGCAGCGATGACCAGGGGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGAGCTTAGGtata                                                                                                                                                                               <  1:42724/251‑1 (MQ=255)                                                                cACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATAGCGAAGAAGGTTAACACCCACACCCACGGCGGCCCTTCCGGGAAGAGGGCGGGGAGATAGATTTTCGCCAAAAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCCGCCGACCATAAATCCCACGTGCTGGTTAATCGGCTTTTTCGCGTAGGGTTAGGCCGCAACCg                                                                                                                                                                    >  2:144081/1‑251 (MQ=255)                                                                             aGGGTACTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGGATAAGCCG‑AACCGGCCTCCGGAAACTg                                                                                                                                                      >  1:128041/1‑251 (MQ=255)                                                                                   ttGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTAACAACCACCCCCCCGGCGGCCCTTACGGGACGAGGGCGGAGAGATAGCTTTTCGCCAAAAAGACGTTAATCATCGGCCAAAAGAGACACTACAGCAGCGATGACCAACCGACCATAAATCCGACGTGCTGGTTAATACCCTTTTTCGCGTAGGTATAGGCCG‑AACCGGCCCCCGGAAACTGGCGAAc                                                                                                                                                >  2:275586/1‑251 (MQ=255)                                                                                          tAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATAGCGACGAAGGTTACCACCCACCCCCACGGAGGCACTTCCGGGAAGAGGGAGGGGAGATAGATTTTTGCCAACAAGACGTTAATCATCGGCCACAAGAGACAACCCAGCAGCGGTGACCAGCCGCCCATAAATCCGACGTGCTGGTTAATCCACTTTTGCGCGTAGGTATTAGCCG‑CACCGGCCTCCGGAAACCGACGAACCAGtatg                                                                                                                                         >  2:82368/1‑248 (MQ=255)                                                                                              gACCAGGTTGACGCCCTTCAGATTCGCGGCGGATAAAATGGCGACGAAGGTTAACACCCACACCCCCGGCGGGACTTCCGGGAAGAGGGCGGAGAGATCGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGGTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCAAACCAGGTTggcgc                                                                                                                                     >  1:150574/1‑247 (MQ=255)                                                                                                               tcggATTCGCGGGGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTACTCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAGCTGGCGAACCAGTTTGCCGTAGCTGATAGAGGTaaa                                                                                                                     <  2:93867/247‑1 (MQ=255)                                                                                                                        cggcggTTAAAATGGCGACGAAGGGTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCCGTTTGCCGTAGCTGATAGCGGTAAACAGCACAccc                                                                                                           >  2:192322/1‑251 (MQ=255)                                                                                                                               aaaaaTGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAACCACCGGAAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCcgccagcg                                                                                                    <  1:39790/250‑1 (MQ=255)                                                                                                                                           gagggtgaccaccCACACCCGCGGAGGCACTTCGGGGAAGAGGGGGGAGAGATAGATTTTCGCAAACAAGACGTTAAGCAGCGGAAAAAAGGGACAATCCAGGAGGGAGGTCCAGCGGAGGATAAATCCGATGTGGGGGTTAAGCGACTTGTGCGCGTGGGTATAGGCCG‑AACCGGCGGCTGGAAACTGGCGACCCAGTGTGCTGTGTGTGATATGGTTAAACAGCACACCCGCCAGCGCCAGCAAATAgg                                                                                        <  2:41706/244‑1 (MQ=255)                                                                                                                                                               ccggcggCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACTTTAATCACCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGCCTTTTGCGCGTAGTTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCg                                                                    <  1:146644/250‑1 (MQ=255)                                                                                                                                                                           ccGGGAAGAGGGCGGAGATATAGATTTTCGCCAACAAGACTTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGCCCAGCGGACCATAAACCCGACGTGCTGGTTAATCGACTTTTGCGGGTAGTAAAAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCCCCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGgag                                                        <  1:416531/251‑1 (MQ=255)                                                                                                                                                                             gggAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCGGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCCCCCCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGAc                                                      <  1:448906/251‑1 (MQ=255)                                                                                                                                                                                        ggAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAg                                           <  1:192322/251‑1 (MQ=255)                                                                                                                                                                                                    tttCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACgg                               >  1:451159/1‑251 (MQ=255)                                                                                                                                                                                                      ccgcaaacaaGACTTTAATCACCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTGGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCATCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGt                              <  1:305764/245‑1 (MQ=255)                                                                                                                                                                                                          caacaaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGCCGCTAATGCCGGAGACAATACCAAAAGAATCAAATACGGTCATCg                         >  2:159940/1‑251 (MQ=255)                                                                                                                                                                                                           aacaaGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCgg                        >  1:420752/1‑251 (MQ=255)                                                                                                                                                                                                               aGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTCGGTATAGGCCG‑CACCGACCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTg                    >  1:290441/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaaGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCcatc  >  2:29037/1‑250 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    GCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCG‑AACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGCGTGAGATAGGCCAGACCCATC  >  NC_000913/1358627‑1359101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑