Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	61.5 / NA	19	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/6);  total (13/6)

CGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACA  >  NC_000913/1169613‑1170063                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     cgcACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACt                                                                                                                                                                                                           <  2:444149/251‑1 (MQ=255)                   agagACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTtat                                                                                                                                                                                         >  1:239899/1‑251 (MQ=255)                                   gtgtTGATGATATTCACTTTGGTGCCTGGGGAGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGACGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGtt                                                                                                                                                                         >  1:395667/1‑251 (MQ=255)                                                                  tGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGt                                                                                                                                         >  1:296725/1‑251 (MQ=255)                                                                                 gtgtTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACgggg                                                                                                                          >  1:185899/1‑249 (MQ=255)                                                                                     tttGATATCGTCATCCCGCAGACGAATAAAGCCAGCACTTACCCGCATGCCAATGCCGACATCGGCGTTCGTACCATGAAGCAAATCACCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTc                                                                                                                       >  2:411029/1‑250 (MQ=255)                                                                                      ttGATAGCGTCATCCCGCAGACGACTACAGCCAGAACTTACCCGCATGCCAATTCCGAAATCGGCGTTCGTACCATGAAGCAAATAACCGCCGCCAGAGGCCTCCAGACGAAGCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCATCTCAATTCCCTGTGCCTGATAACTGTCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑GTGCACGTTTGTCTGTCACGGTGGTCa                                                                                                                      <  2:288691/250‑1 (MQ=255)                                                                                         atatCGTAATCCCCCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTAACATGACGCAAATACACGCCGCCCTAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCACTTCCCTGTGCTTTATCACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGACACGGTGGTCCCCAt                                                                                                                  >  2:20560/1‑251 (MQ=255)                                                                                                 aTCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGgtg                                                                                                          >  2:368262/1‑251 (MQ=255)                                                                                                    ccGCAGCCGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGACGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAAAGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCCTTGTCGGTGTc                                                                                                        >  1:403031/1‑250 (MQ=255)                                                                                                       cAGACGAATACAGCCAGAACTTACCCGCATGCCAATCCCGAAATCGCCGTTCGTACCATGAACCAAATAAACACCGCCATCGACAGCCAGACGAATCACATCATGGCGCATTGGGATATCCGGTCCAGCCGGCACTACCGCAGGCAACTCCACTCCCCGCGCTTTATAACCGGCACGGACCGCTGCCGTAGGCGTCC‑TGTTCAGTACTGCACGTTTATCTGCAACGGTGGTCAACATTTTCGgtgccgg                                                                                                      >  2:217651/1‑245 (MQ=255)                                                                                                                  aGCCAGAACTGACCCGAATGACAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGGCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCTCCACCt                                                                                         <  2:365124/251‑1 (MQ=255)                                                                                                                    ccAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGAAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTa                                                                                        >  1:306683/1‑250 (MQ=255)                                                                                                                                   aTGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCACATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTAt                                                                        >  1:216104/1‑251 (MQ=255)                                                                                                                                                        ttCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAAtt                                                   >  1:249841/1‑251 (MQ=255)                                                                                                                                                                        aaTAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCgggg                                     >  2:91196/1‑248 (MQ=255)                                                                                                                                                                                 cgccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTCCCCGGCGGGTAGTAATAgaga                          <  2:111169/251‑1 (MQ=255)                                                                                                                                                                                                  cGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGtt         <  2:101426/251‑1 (MQ=255)                                                                                                                                                                                                         cATGATGGCACATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTTAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGTCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGGCAGCGTGTCACCACCTAACTGACCAATACCTATGGGATACACGGTTACCGAAGTTTTACCCGGCGGGTAGTAATAGAGATGCAGCTCCGCAATGTTGATcaca  <  1:290429/251‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                     CGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGT‑TTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACA  >  NC_000913/1169613‑1170063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑