Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	696,470	C→T	noncoding (35/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	696,470	0	C	T	100.0%	42.2 / NA	15	noncoding (35/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/8);  total (7/8)

TGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTG  >  NC_000913/696239‑696692                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               tGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCg                                                                                                                                                                                                             >  1:327309/1‑251 (MQ=255)      cGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTgg                                                                                                                                                                                                        >  2:12896/1‑251 (MQ=255)       gTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGc                                                                                                                                                                                                       >  1:419886/1‑251 (MQ=255)        tCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCg                                                                                                                                                                                                      >  2:88138/1‑251 (MQ=255)                                      gCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTaa                                                                                                                                                                        <  2:419886/251‑1 (MQ=255)                                                                      gaGGTAGGATAAGACGCGGCAGCGTCGCAGCCGACATAGAAGGATAAGACGTGGCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGa                                                                                                                                        <  2:110621/250‑1 (MQ=255)                                                                           cGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGGGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTTCGAGGATTCg                                                                                                                                    >  2:79898/1‑250 (MQ=255)                                                                                                  agccGACAGTGAAGGATAAGACGTGGCAACAGCGCATGCGAAATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGGTTTGTGAATGTGGCTGTGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACGGGGTGCACTTACAAGGGAAGCGTCTGGAATAAATTGGCTGGGGTACGAGGAGTCGAACCTCGGAATGCCTGAATCAGaa                                                                                                            <  2:195386/249‑1 (MQ=37)                                                                                                          ttGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGAACTTACAAGGTAAGCGGCTTGAATAAATTGGCTGGGGTACGAGGATTCTAACCTCGTAATGCCGACATCAGAATCCGGTGc                                                                                                    >  2:287927/1‑251 (MQ=38)                                                                                                                 aTAAGACGGGTCAACATCGCATTCGACAGTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTGTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGACGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTGACAAGGTAAGCGTCTTTAATAAAGGGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCg                                                                                             <  2:277413/251‑1 (MQ=38)                                                                                                                                              tgaatgaaCGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGtttt                                                                 <  1:12896/250‑1 (MQ=255)                                                                                                                                                                   aaaGCTCGCCGAAGCGAGCTTTTTTAATGGGGCTGGGGTACGAGGATTCAAACCTCGGAATGCCGGAATTAGAACCCGGTGCCTGACCGCTTGGCGATACCC‑AACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTTGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATa                                          <  2:463651/251‑1 (MQ=38)                                                                                                                                                                                              ggtgGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACgg                <  2:98615/250‑1 (MQ=255)                                                                                                                                                                                               gtgGCTGGGGTACGAGGATTCTAACCTCGGAATGCCGGAATTAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTGACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAGTGGTTTTGAAGTTGCGGAACATATTCGATACATTCAGAATTTGGTGGCTACGACgg                <  1:204488/250‑1 (MQ=255)                                                                                                                                                                                                            gaggaacctaaCCTCGGAATGCCGGAATTAGAATCCGGCGCCTTACCGCTTGGCGATACCCCAACTAGGTGCACTTACAAGGTAAGCGCCTTGAATAAATTGGCTAGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGGGCCTTACCGCCTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGTTACGACGGGGTTCGAACCtgtg  >  1:321071/10‑251 (MQ=38)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                               TGAAACGTCAGGCGCTGAAATATGCGTTAGGGTTGTAGCCTTACAACATTGCCGGATGCGTGCCAACCGTAGGTCGGATAAGACGCGGCAGCGTCGCATCCGACATTGAAGGATAAGACGTGTCAACATCGCATTCGACATTGAATGAACGCAGAAAAGCAAAAAGCTCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTG  >  NC_000913/696239‑696692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑