Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	1,302,405	Δ4 bp	coding (1224‑1227/1632 nt)	oppA →	oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1302404	1 (0.020)	28 (0.630)	25/482	0.6	96.6%	coding (1223/1632 nt)	oppA	oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1302409 =	1 (0.020)					coding (1228/1632 nt)	oppA	oligopeptide ABC transporter periplasmic binding protein

TTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCT  >  NC_000913/1302167‑1302651                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   attgttaataaagtgaaagcgcagggcaacatgcccgcctatggttacactccaccgtatactgatggcgcaaaattgactcagccagaatggtttggctggagccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgctctgcataaaaagctggcgattgctgcctcttaattgtgAAAACCAtt                                                                                                                                                                                                                                            >  2:152380‑M2/243‑251 (MQ=255)               aaaagggcagggcaacaggcccgcctatggttacactccaccgtatactgatggcgcaaaagtgactcagccagaatggtttggctggagccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcctcattgtgAAAAACATTGGTGTAAACGTCa                                                                                                                                                                                                                               <  2:265951‑M2/22‑1 (MQ=255)                                    cgcctatggttacactccaccgtatactgatggcgcaaaattgactcagccagaatggtttggctggagccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGa                                                                                                                                                                                                          >  2:159126‑M2/208‑250 (MQ=255)                                     gcctatggatacactccaccgtatactgatggcgcaaaattgactcagccagaatggtttggctggagccaggaacaacgaaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGCGTGgac                                                                                                                                                                                                         >  2:397129‑M2/207‑249 (MQ=255)                                        tatggttacactccaccgtatactgatggcgcaaaattgactcagccagaatggtttggctggagccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACg                                                                                                                                                                                                     >  2:9368‑M2/204‑251 (MQ=255)                                                   tccaccgtatactgatggcgcaaaattgactcagccagaatggtttggctggagccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGacac                                                                                                                                                                                          >  1:313533‑M2/193‑251 (MQ=255)                                                              ctgatggcgcaaaattgactcagccagaatggtttggctggagccaggacacacgtaccgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAgg                                                                                                                                                                               >  1:234986‑M2/182‑251 (MQ=255)                                                                   ggcgcaaaattgactcagccagaatggtttggctggagccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTAc                                                                                                                                                                           <  1:424803‑M2/74‑1 (MQ=255)                                                                   ggcgcaaaattgactcagccagaatggtttggctggagcaaggaaaaacgtaacgacgaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACCTTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACt                                                                                                                                                                          >  2:274428‑M2/177‑251 (MQ=255)                                                                              ggctcagccagaatggtttgtctggagccaggaaaaacgtaacgaagaatcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTgatgttg                                                                                                                                                                <  2:253413‑M2/85‑3 (MQ=255)                                                                                 ccagccagaatggtttggctggagccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCg                                                                                                                                                            <  2:313533‑M2/89‑1 (MQ=255)                                                                                             gtttggctggagccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcctaaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGAGGAAAACGTTCCTCGCCACCCGTCACCAGGGTCCTTTTGATGAGGCCCGTGCCGGctgggg                                                                                                                                                >  2:239589‑M2/151‑249 (MQ=255)                                                                                                     ggagccgggaaaaacgtaacgaagaagcgaaaatactgctggctgaagcgggatataccgcagacaaaccgttgaccatcaacatgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACt                                                                                                                                        >  1:368005‑M2/143‑251 (MQ=255)                                                                                                       aaccaataaaaacttcactaataagcaaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgagctgcataaaaagctggcgattgctgcctcttcattttgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTtactac                                                                                                                                      <  2:229448‑M2/111‑1 (MQ=255)                                                                                                         ccaggaaaaacgtaacgaagaagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGCACTTTTGATGTGGCCAGAGCAGGCTGGTGTGCTGAATACa                                                                                                                                     >  2:244915‑M2/139‑250 (MQ=255)                                                                                                          caggaaaaacgtaacgaagaagcgaaaaaactgctggttgaagcgggttataccgcagacaaatcgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAAc                                                                                                                                   <  2:368005‑M2/114‑1 (MQ=255)                                                                                                                             aagcgaaaaaactgctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTg                                                                                                                 >  1:404223‑M2/119‑250 (MQ=255)                                                                                                                                          gctggctgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCttt                                                                                                    <  1:108828‑M2/145‑1 (MQ=255)                                                                                                                                                tgaagcgggttataccgcagacaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAAc                                                                                               >  1:196316‑M2/100‑249 (MQ=255)                                                                                                                                                    gttatagcgtacacccaCCGTTGACCATCTGCCTGTTGTATAAAAACTCCGATCTGCCTAAAAAGCCGGCGATTGCTGCCTCTTCATTG‑‑‑‑AGCAAACCCATGGTGTAAACGTCACACTGGTTAACCAGGAGTGGAACACGTTCATCGACAACCGTCACCCGGGTACTTTTGATGTGGCCCGTGCAGGCTGGCGTGCTGCCTACAACGCACAAAATTCCTTCCTGCACACCATCCTTTCGAACAGCCCGATg                                                                                      >  2:69383/17‑250 (MQ=255)                                                                                                                                                             accgcagacaaaccgttgaccagcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATAc                                                                                 <  2:382265‑M2/164‑1 (MQ=255)                                                                                                                                                                    acaaaccgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCAAACAGCTCGATGAATACCGCGCAtt                                                                         <  1:310305‑M2/172‑1 (MQ=255)                                                                                                                                                                          cgttgaccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATtata                                                                      >  2:114250‑M2/74‑248 (MQ=255)                                                                                                                                                                                ccatcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCgg                                                             <  1:170138‑M2/184‑1 (MQ=255)                                                                                                                                                                                  atcaacctgttgtataacacctccgatctgcataaaaagctggcgattgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGcc                                                           <  1:26440‑M2/186‑1 (MQ=255)                                                                                                                                                                                                           atctgcataaaaagctggcgatcgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCTGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGACCGGCCTTTGACAGCATTATGGCGGAAACGc                                  >  1:366403‑M2/41‑251 (MQ=255)                                                                                                                                                                                                                               attgctgcctcttcattgtgAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGgcg              <  2:404223‑M2/231‑1 (MQ=255)                                                                                                                                                                                                                                           tcattgtgAACAACATTGGTGTCAAAGTCAAACTGGTTAACCAGGAGTGGAAAACGATCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCACCTTCCTTCCTGAACACCATGCTTTCGCACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGAGGAAACGCTGAAGGTGACTGACGAGGCGCAGCGCACCGct  >  2:313031‑M2/9‑251 (MQ=255)                                                                                                                                                                                                                                           tcattgtgAAAAACATTGGTGTAAACGTCAAACAGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGTTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGAAAGCATTATGGCGGAAACACTGAAAGTGACGGACGAGGCGCagcgcaccact  >  1:21174‑M2/9‑247 (MQ=255)                                                                                                                                                                                                                                                  gaaAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCt                                 <  1:251784/213‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   TTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTGACTCAGCCAGAATGGTTTGGCTGGAGCCAGGAAAAACGTAACGAAGAAGCGAAAAAACTGCTGGCTGAAGCGGGTTATACCGCAGACAAACCGTTGACCATCAACCTGTTGTATAACACCTCCGATCTGCATAAAAAGCTGGCGATTGCTGCCTCTTCATTGTGGAAGAAAAACATTGGTGTAAACGTCAAACTGGTTAACCAGGAGTGGAAAACGTTCCTCGACACCCGTCACCAGGGTACTTTTGATGTGGCCCGTGCAGGCTGGTGTGCTGACTACAACGAACCAACTTCCTTCCTGAACACCATGCTTTCGAACAGCTCGATGAATACCGCGCATTATAAGAGCCCGGCCTTTGACAGCATTATGGCGGAAACGCTGAAAGTGACTGACGAGGCGCAGCGCACAGCT  >  NC_000913/1302167‑1302651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.