Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,933,292	IS1 (+) +9 bp	intergenic (+108/‑51)	kup → / → rbsD	K(+):H(+) symporter Kup/D‑ribose pyranase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1979270	NA (NA)	53 (1.200)	49/482	0.1	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 3933300	0 (0.000)					intergenic (+116/‑51)	kup/rbsD	K(+):H(+) symporter Kup/D‑ribose pyranase
*	?	NC_000913	= 3584195	NA (NA)	43 (0.970)	39/484	0.2	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	3933292 =	0 (0.000)					intergenic (+108/‑59)	kup/rbsD	K(+):H(+) symporter Kup/D‑ribose pyranase

CCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCA  >  NC_000913/1979022‑1979272                                                                                                                                                                                                                                                         |   ccAACGCACTTCATGGCAATAACAATGATTTTCTGGTGCGGACAGGGTTGAGAAGCGGTGTAAGTGAAATGCAGATGCCATGTTTTACGGCAGTGAGAGCAGAGCTAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTAAGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCCGATAGAAACAGAAGCCACTGGAGCACCTCAAAAGCACCATCATTCACTAAACCTGTAAGTTGGCAGCATCAcccc  <  2:21302/251‑2 (MQ=11)                                                                                                                                                                                                                                                         |   CCAACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCCA  >  NC_000913/1979022‑1979272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.