Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (+589/+167)	rtcR → / ← glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	194.4 / NA	56	intergenic (+589/+167)	rtcR/glpG	DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (28/28);  total (28/28)

TTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGA  >  NC_000913/3560212‑3560689                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           tttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAt                                                                                                                                                                                                                                      >  1:155315/1‑251 (MQ=255)  ttACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcgcaggctc                                                                                                                                                                                                                                     >  1:386546/1‑243 (MQ=255)    cccATCAACGTGTTAGAAAAGTTGATATTGTTGGTGACATGGCGCAAATTGCTGTGATGGAGCAGTGCGTG‑CGCTCCCGCTCCCGGTGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CCCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCa                                                                                                                                                                                                                                    <  1:324844/249‑1 (MQ=255)           aCGTGTTAGCAACGTTGAGATTGTTGGTGACAATGAGCAAATTGCTGTGATTGAGAAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTGACCGAACTGGAAGGCAGCGCCGCACCGCCCTGATGGCGCAGGATCAGGTTTTGc                                                                                                                                                                                                                            >  2:195457/1‑251 (MQ=255)                  aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGcc                                                                                                                                                                                                                      >  1:426514/1‑250 (MQ=255)                        gTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTATGGGTGGCCTTGCGATCGTGCCACGGCGTGATAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                               >  1:88114/1‑251 (MQ=255)                         ttGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGAAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                              >  1:330185/1‑251 (MQ=255)                            agaTTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGCTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTg                                                                                                                                                                                                             >  1:171555/1‑249 (MQ=255)                                   ggggaacatggcgaaaaTTGCTGGGATTGAGCGGTG‑GTGGCGCTACCGCTTCCGGCGGGGGGCCGATATCGATAAACAGCGTCG‑GCCCATGGGGGTTTG‑CTCCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcgg                                                                                                                                                                                                    <  1:29241/237‑1 (MQ=255)                                       aaCAATGCGCAAATTGCTGTGATTGGGCAGTGCGTG‑CGCTACCGCGTCCGGGGTGGTGCCGATATCGATAAACAGCGTCGAGCCTTGGGGGATTTG‑CTCC‑GCCCCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                                                                                                                                <  1:132362/250‑1 (MQ=255)                                         cAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAAt                                                                                                                                                                                               >  1:190852/1‑250 (MQ=255)                                         aaaggcgaaaaTTGCTGTGATGGAGCAGTGCGTG‑CGCTCCCTCTTCCGGGGTGGTGCCGATATCGATAAACAGCGCCGAGCCTTTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                                                                                                                              <  1:423674/243‑1 (MQ=255)                                              cgcAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTg                                                                                                                                                                                         >  1:433570/1‑251 (MQ=255)                                                aaaattgttggtaTTGAGCAGTGCGGG‑CGCTACCGCTTCCGGGGTGGGGCCGATATCGATAAACAGCGTCGAGCCATTGGGGTTTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                                                                                                                       <  1:181595/239‑1 (MQ=255)                                                aaaaTTGCTGTGAGTGAGCGGGGCGGG‑GGCGCCCGCTTCGGGCGGGGTGCCGATATCGAAAACCGGCGGCGAGCCGTGGGGGTTTGG‑CCCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCg                                                                                                                                                                                       <  2:190852/250‑1 (MQ=255)                                                       cTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                                >  2:87704/1‑251 (MQ=255)                                                       cTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAc                                                                                                                                                                                >  1:248365/1‑251 (MQ=255)                                                                   aGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGAGGCGAATAGTCTGCGGGCTGACGGAGAAATGctc                                                                                                                                                                    >  1:349957/1‑251 (MQ=255)                                                                               tACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                         >  2:321986/1‑251 (MQ=255)                                                                               tACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGATATGCTCCGCCAGCTCATTGAGGTAGCGGCGAATAGTCTGAGGGCTGACGGAGCAATGCTCTACAAGCTCtt                                                                                                                                                         >  2:19008/1‑251 (MQ=255)                                                                                cccgctccggggggggtgCGGATATCGAAAAACAGCGTCGACCTATGGGGGTTTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGGGCCACGGCGTGTTAACCGAACTGGAGGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTc                                                                                                                                                        <  2:433570/237‑1 (MQ=255)                                                                                cccGCTTCGGGCGTGGGGCCGATAGCGATAAACGGCGCCGAGCCATTGGGGTTTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctct                                                                                                                                                          <  1:10879/248‑1 (MQ=255)                                                                                    cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑CCCACTTTGCGAGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGCAATGCTCTACCAGCTCTTCGGt                                                                                                                                                     >  1:410170/1‑250 (MQ=255)                                                                                        cGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGACCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTg                                                                                                                                                 >  1:296888/1‑250 (MQ=255)                                                                                           cGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt                                                                                                                                             >  1:116079/1‑251 (MQ=255)                                                                                                       agcGATAAACAGTGGCGAGCCATTGGGGTTTGG‑CTCC‑GCCACTTGGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCGCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                                 <  1:443235/249‑1 (MQ=255)                                                                                                         gtgaaacacggggtcgagctgtGGGGGTTTGGCTCC‑GCCTCTTGGCGGGCGATGCGCTTTTTTTCTCCGGGCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGtt                                                                                                                                 <  2:7266/229‑1 (MQ=255)                                                                                                           aTAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCAATTTGCGGGCGATGCGCTCTTTTTCTTCCGTCTGGGTGGCCCTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCCTGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGCGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                                                                                                                             >  1:434009/1‑251 (MQ=255)                                                                                                             agcgtgctcggagctatgggggTTTTG‑CTCC‑GCCATTGTGCGGGCGTGGCCCTCTTTTTCTTCGGTCTGGGTGGCGTGGCGCTCGTGCGAGGGCGTGTTAACCGACCGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGAGCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTTCGGTTTAACCa                                                                                                                           <  2:434748/233‑1 (MQ=255)                                                                                                              aaCAGCGACGAGCCCATGGGGATTTG‑CTCC‑GCCACTATCCGGGCGACGCGCTCTTTTTCTTCGGTCTCGGCGGCCTTGCCATCATGCCACGGCGTGTTAACCGAACTGGAAAGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAACTGCTCTACCAGCTCTTCGGTACTGACATAACACTGCTGTTTAAcc                                                                                                                            >  1:405619/1‑249 (MQ=255)                                                                                                                gggcgtggggctatgggggATTTG‑CTCC‑GCCACTGTGGGGGCGATGCGCTCTTTTTCTTCGGGCGGGGGGGCCTGGCGAGCGGGCCACGGCGTGTAAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                                        <  2:171555/236‑1 (MQ=255)                                                                                                                        aGCCATTGGGCATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGCCTGGGTGGCCTTGCGATCGTGCCACGCCGTGTTAACCGCACTGGAAGGCAGCGACGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCATTGAGGTCGCAGCGCATCGTCTGCGGGCTGACGGAGAAATACTCTACCGCCTCCTCGGTCCTGCCATCACCCAGTTGTTTatacagatatataat                                                                                                                 >  2:296365/1‑236 (MQ=255)                                                                                                                                 gggttttggcccctcctcTTTGGGGGCAATGCGTTTTTTTTTTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTtgt                                                                                                         <  2:175989/234‑1 (MQ=255)                                                                                                                                   aTTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCTGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACCTAACCCTGCTGTTTAACCAGTTCGATAATAACGTTGTGACg                                                                                                     >  1:86498/1‑251 (MQ=255)                                                                                                                                   aTTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGATGTGACg                                                                                                     >  2:237196/1‑251 (MQ=255)                                                                                                                                              gccacttgggggggggtGCGCTCTTTTTCTTCGGTGGGGGTGGCCTTGGGATGGTGCCGCGGGGTGTAAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGtgtttg                                                                                            <  2:386546/235‑1 (MQ=255)                                                                                                                                                    ttGCGGGCGTGGCGCTCTTTTTCTTCGGTCTGGGGGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                                       <  1:38955/250‑1 (MQ=255)                                                                                                                                                    ttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                                        <  1:23818/249‑1 (MQ=255)                                                                                                                                                    ttGCGGGCGATGCGCTCTTTTGCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCa                                                                                       <  1:223004/250‑1 (MQ=255)                                                                                                                                                        gggCGATGAGATCTTTTGCTTCGGTCTGGGTGGACTTGAGATCGTGCCACGGAGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGGTCAGGTTTTGCTCCGACAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCGGACGGAGAAATGCACTACCAGCTCTTCGGTACTGACATAAACCTGCTGTTTAACCAGTTCGATAATACCGTTGGGCAGTTGGGTTTGTTTCATTta                                                                                   >  2:338693/1‑250 (MQ=255)                                                                                                                                                          gCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                               >  2:181634/1‑124 (MQ=255)                                                                                                                                                          gCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAt                                                                                                                                                                                                               <  1:181634/124‑1 (MQ=255)                                                                                                                                                           ggttGCGCTCTTTTTCTCCGGTCTGGGTTGCCTTGCGATCGTGCCACGTGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCTCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCGCTACCAGTTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTGGATAATACCTTTGTGACGTTGTGTTTGTTTCATTTATaaa                                                                               <  1:281810/248‑1 (MQ=255)                                                                                                                                                            gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAAt                                                                              <  1:311654/251‑1 (MQ=255)                                                                                                                                                                            ggggTCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAattatt                                                                 <  2:14877/246‑1 (MQ=255)                                                                                                                                                                              ggTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgt                                                            >  1:350047/1‑251 (MQ=255)                                                                                                                                                                                   gggTGGCCTTGCGATCGTGCCCCGGCGTGTTAACCGAACGGGAAGGCAGCGCGGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAttttcgttttcg                                                       <  2:248365/251‑1 (MQ=255)                                                                                                                                                                                    ggTGGCCTTGCGACCGGGCCACGGGGTGTTACCCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAGTTTCGTTTTcgc                                                      <  2:258175/251‑1 (MQ=255)                                                                                                                                                                                     gTGGCCTTGCGATCGTGCCACGGCTTGTTAACCGAACTGTAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATTCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTcgcg                                                     <  1:92166/251‑1 (MQ=255)                                                                                                                                                                                            tgcgtgctggCCACGGCGGTTTAACCGAACTGCAAGGCAGCGCCGCACCGCCTGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGCCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTATCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGCTGTGTTGGTTGCATTTATAAATCCCTGGAATTATGTTCGTTTTCGCGCATTg                                                <  2:471309/240‑1 (MQ=255)                                                                                                                                                                                                    tGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATca                                       >  1:374220/1‑250 (MQ=255)                                                                                                                                                                                                       cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaacaa                                   <  1:320780/251‑1 (MQ=255)                                                                                                                                                                                                         gggCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa                                 <  2:235196/250‑1 (MQ=255)                                                                                                                                                                                                            cGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGAAGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                              >  1:168406/1‑251 (MQ=255)                                                                                                                                                                                                                             aagtgaGCGCCTCTCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAAGCCCTGGAATTATTTTCGTTTTCGCGCATTTAGCGAATCAACAAAAGCCATCGCTAAACCCACGGc             <  1:367217/246‑1 (MQ=255)                                                                                                                                                                                                                                      ccgcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCATGCTGTTTAAACAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc    >  1:214911/1‑251 (MQ=255)                                                                                                                                                                                                                                        gcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATAGCTAAACCCACGGCTAACCCGGCgc  >  1:362357/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                           TTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGATTGAGCAGTGCGTG‑CGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTG‑CTCC‑GCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGA  >  NC_000913/3560212‑3560689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑