Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,207,549	Δ1 bp	coding (179/606 nt)	ttdB →	L(+)‑tartrate dehydratase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,207,549	0	G	.	100.0%	317.5 / NA	68	coding (179/606 nt)	ttdB	L(+)‑tartrate dehydratase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (34/34);  total (34/34)

CGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGA  >  NC_000913/3207306‑3207789                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cggtgctcgcggcgggTTAATGCCGATCTCACCTTTGAAAATCTTCCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGCCACCCCCGACCAAAGCGGAAGTTCTGCAAGATATGCGGGTCGGCGAGGTGATCTGCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCg                                                                                                                                                                                                                                          <  2:409170/237‑1 (MQ=255) cgcgGCGCGCTGCTTGTTCATGCCGCCCTCACCTATGAAAACCTGCCCCACCCCCGGATCGCGTTATGAAAAAGTTCCTGACAACCCCGATCAAAGCCGAAGATCTGCAAGTGATGCCGGGCGGCTATGATTTCTACCGGACCGATACGCTGGTGACCTGCCGCGACGTT‑GTCCTCCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCg                                                                                                                                                                                                                                          <  1:138939/251‑1 (MQ=255)        cgcggctgGTCCATGCCAATCTCACCTTTGAAAATCTGTCCCCCCCCCGGAGCGCGTTATGAAAAAGACCCTGACAACCCCGATCAAAGCGGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGCCCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaa                                                                                                                                                                                                                                   <  2:155149/247‑1 (MQ=255)         gcggctgGTTCATGCCGATCTCACCTTTGAAAATCGGTCGCACGCCTGGAGCGCGTTATGAAAAAGATCCGGACAACCCCGATCAAAGCGGAAGATCTGCAAGGTATTCGCGTCGGCGATGTGTGCTACCTGGCCGGTACGCTGGTGACCTGCCGCGACGTTTGGC‑TCCGCCGTTTGAGCGAACTGAAACGGCCGATCCGTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaa                                                                                                                                                                                                                                  <  1:44699/248‑1 (MQ=255)          ctgctgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaa                                                                                                                                                                                                                                  >  1:77355/1‑250 (MQ=255)           tgctgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCaaaa                                                                                                                                                                                                                                 >  1:30238/1‑250 (MQ=255)              tgGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGCGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACgg                                                                                                                                                                                                                             >  1:27928/1‑251 (MQ=255)                          aTCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATggg                                                                                                                                                                                                                  >  1:208776/1‑250 (MQ=255)                           tctcACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCAGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGACACGTCCGATCCCTTACGATCTCAACGGAAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGAAAAATggg                                                                                                                                                                                                                  >  1:23686/1‑249 (MQ=255)                              cACCTTTGAAAACATGCCTCACACCCGGATCGCGTTATGAAAAAGACCCTGACAACCCCGATCAAAGCTGAAGAGCTGCAGGATATCCGCGTCGGCGTTGTGATCTACCTGACCGTTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGGCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATg                                                                                                                                                                                                             <  2:378/251‑1 (MQ=255)                                       aaaTCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTc                                                                                                                                                                                                    <  1:470944/251‑1 (MQ=255)                                        aaTCTGTCTCACACCCGGAGCGCGCTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCg                                                                                                                                                                                                   >  2:279349/1‑251 (MQ=255)                                             gTCTCACCCCCGGAGCGCTTTATGAAAAAGATCCTGACACCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGGTGTGATCTACCTGACCGGTACGCTGGGGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCTTCGGccc                                                                                                                                                                                               <  2:280438/250‑1 (MQ=255)                                              tctcACACCCGGAGCGCGTTATGAAAAAGATCCTGACCACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCg                                                                                                                                                                                              >  1:213541/1‑250 (MQ=255)                                                   cccccGGAGCGCGTAATAAAAAAGACCCTGACACCCCCAATCAAAGCGGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAcc                                                                                                                                                                                        <  2:106108/249‑1 (MQ=255)                                                   cacCCGGAGCGCGTTATAAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATGCGCGTCGGCGAGGTGTTCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAAcc                                                                                                                                                                                        <  1:23878/251‑1 (MQ=255)                                                     cccGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACaccc                                                                                                                                                                                        >  2:326727/1‑246 (MQ=255)                                                     cccGGAGCGCGTTATGAAAAAGACCCTGAAACCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGTTGTAATCTACCGGACCGGTACGCTGGTGCCCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGAGGGTCTCCGTCGGCCCGACAACCAg                                                                                                                                                                                      <  2:23686/251‑1 (MQ=255)                                                     cccGGAGCGCGTTAGGAAAAAGATCCTGACAACCCCGATCAAAGCGGAATATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGCCCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGGGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAg                                                                                                                                                                                      <  1:30518/251‑1 (MQ=255)                                                      ccGGAGCGCGTTATGAAAAAGATCCTGACACCCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGCTGAGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGCCGTTTGTC‑ACCGCCGTTTGCTCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAACCGGCGACAAATGGGCGATGGTCTCCGGCGGccagccaccccgt                                                                                                                                                                                     >  2:441786/1‑240 (MQ=255)                                                      acggaccacgtaatgccaaaGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGCTGTGATCTACCTGCCCGGTCCGATGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCCAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGCTGGTATCCGTCGGCCCGACAAcccgg                                                                                                                                                                                     >  2:232982/18‑248 (MQ=255)                                                      acGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAgg                                                                                                                                                                                     >  2:285446/2‑250 (MQ=255)                                                        ggAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCGGAAGATCTGAAAGATATTGGCGTCGGCGAGGTGATCTACCTGACCGGTACGCTGGTGACCGGCCGCGACGTTTGGC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTAt                                                                                                                                                                                   <  1:472529/251‑1 (MQ=255)                                                            cgcgTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGga                                                                                                                                                                                                                 >  2:274214/1‑217 (MQ=255)                                                            cgcgTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCGGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGga                                                                                                                                                                                                                 <  1:274214/217‑1 (MQ=255)                                                             ccgTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGAGCGGCAAGATATGCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACATATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTa                                                                                                                                                                              <  1:196988/250‑1 (MQ=255)                                                                ttATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAc                                                                                                                                                                                            >  2:172231/1‑234 (MQ=255)                                                                ttATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGAc                                                                                                                                                                                            <  1:172231/234‑1 (MQ=255)                                                                 tATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATgg                                                                                                                                                                           >  2:328378/1‑250 (MQ=255)                                                                     aaaaaGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTCCCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGt                                                                                                                                                                      <  1:364945/251‑1 (MQ=255)                                                                      aaaaGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGtt                                                                                                                                                                     >  1:452407/1‑251 (MQ=255)                                                                        aaGATCCTGACAACCCCGATCAAAGCGGAAGATCTGCAAGATCTTCGCGTCGGCGATGGTACCTACCGGTCCGGTTCGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGAGCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGTAGATGGGCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGtttt                                                                                                                                                                   <  2:265814/251‑1 (MQ=255)                                                                             ccTGACACCCCCGATCAAGGCTGAAGTTCTGCAAGATATCCGCGTCGGCGATGTGTTCTCCCTGCCCGGTACGCTGTTGACCTGCCGCGACGTTGGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAAc                                                                                                                                                               <  2:350867/250‑1 (MQ=255)                                                                             ccTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACg                                                                                                                                                              <  1:328378/251‑1 (MQ=255)                                                                                  cAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGAATGCGTATGGAAAGTTTTGAACGTGAAt                                                                                                                                                         >  1:290417/1‑251 (MQ=255)                                                                                  cAACCCCGAGAAAAGCTAAAGATCTGAAAGATATTCGCGCCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCTACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTGTGAACGTGAAt                                                                                                                                                         <  2:429512/251‑1 (MQ=255)                                                                                     ccccGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGTTACGCTGGTGACCGGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTa                                                                                                                                                      <  2:353203/251‑1 (MQ=255)                                                                                     ccccGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAAttt                                                                                                                                                       >  1:6601/1‑250 (MQ=255)                                                                                        cGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGACTTTATTg                                                                                                                                                   >  2:271205/1‑251 (MQ=255)                                                                                         gATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTg                                                                                                                                                   >  1:35253/1‑250 (MQ=255)                                                                                             aaaGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGCATTTATTGAGc                                                                                                                                                >  2:125232/1‑249 (MQ=255)                                                                                             aaaGCTGAAGATCTGAAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAACACGGCGACAAATGGGAGATGGTCTCCGTCGCCCCGACAACCAGTATGCGAATGGCAAGATTTGAACTTGAATTTATTGAGc                                                                                                                                                >  2:66679/1‑249 (MQ=255)                                                                                                   gAAGATCTGCAAGATATGCGGGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGCCCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGc                                                                                                                                        <  1:464559/251‑1 (MQ=255)                                                                                                   gAAGATCGGCAAGATAGTCGCGTCGGCGATGTGATCTACCGGACCGGTACGCTGGTGACCTGCCGCGACGTGTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCgg                                                                                                                                         <  1:201559/250‑1 (MQ=255)                                                                                                           gCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACt                                                                                                                                >  1:193816/1‑251 (MQ=255)                                                                                                           gCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGACCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGaa                                                                                                                                   <  1:173512/248‑1 (MQ=255)                                                                                                           ccAAGATATTCGCGTCGGCGATGTAATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACt                                                                                                                                <  1:288240/250‑1 (MQ=255)                                                                                                            cAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTAAGATGGTGACCTGCCGAGACGTTTGTC‑ACCGACGTTTGATAGAAATGACACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGAGAATTTATTGAGCAGACCGGCGTGAACCtg                                                                                                                               >  1:181453/1‑251 (MQ=255)                                                                                                                    tCGCGTCGGCGTTGTGCTCTCCCTGCCCGGTACGCGGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTg                                                                                                                        <  2:47709/250‑1 (MQ=255)                                                                                                                                             aCCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAcc                                                                                              >  1:70289/1‑251 (MQ=255)                                                                                                                                             aCCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGAcc                                                                                              >  2:229778/1‑251 (MQ=255)                                                                                                                                                ggTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCga                                                                                            >  1:388028/1‑250 (MQ=255)                                                                                                                                                           gACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGcc                                                                                 >  2:11874/1‑250 (MQ=255)                                                                                                                                                                 ccGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACAGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGGGGTATGGGGCCGCTGAc                                                                                               >  1:71224/1‑230 (MQ=255)                                                                                                                                                                                   gccgTTGGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCTTGCGCAAAAACGGCGACAAATGGGAGATGGGCTCCGGCGGCCCGACAACCATTATGCGTAGGGAAAGGGTTGAACGTGAAGTTATTGAGCAGACCGGCGTGAAACTGTTGGTTGGCAAAGGTGGTATGGTGCCGCTGACCGGAGAAGGCTGCCAGAAATCCAAGGCGCTACATGTGa                                                         <  2:264647/251‑1 (MQ=255)                                                                                                                                                                                              gAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGc                                               >  1:162100/1‑250 (MQ=255)                                                                                                                                                                                               aaCTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGc                                               >  1:39767/1‑249 (MQ=255)                                                                                                                                                                                                                      gATCTCAACGGAAAAGCGCTTTTCCACGCTG‑CCCCCTCGTGCGCAAAAACGGCGACAAATGGGCGATGGTCTCCGTCGGCCCGACAACCAGTATGAGTATGGACAGTTTTGAACGTGAATTTATTGAGCAGCCCGGCGTGAAACTGGTGGTTGGCAAAGGTGGCATGGGGCAGCTGCCCGAAGAAGGCTGCCAGAAATTCAAGGAGCTACATGTGCTTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAcc                      >  2:263804/1‑251 (MQ=255)                                                                                                                                                                                                                          tcAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAg                                                                                                                  >  2:277256/1‑155 (MQ=255)                                                                                                                                                                                                                          tcAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAg                                                                                                                  <  1:277256/155‑1 (MQ=255)                                                                                                                                                                                                                           cAACGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAAc                       <  1:229778/245‑1 (MQ=255)                                                                                                                                                                                                                            aaCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTg                >  2:243974/1‑251 (MQ=255)                                                                                                                                                                                                                            aaCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCGGCCAGAAATTCAAGGCGCTACATGTGATTGTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTg                <  2:452407/251‑1 (MQ=255)                                                                                                                                                                                                                             aCGGCAAAGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTGGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTgg               <  2:382581/251‑1 (MQ=255)                                                                                                                                                                                                                                    aGCGATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCTCGGCTGGCTGCTCGGTGCTGGCGGCAACCCAGGTGGAAGAGAt        <  1:65288/251‑1 (MQ=255)                                                                                                                                                                                                                                       gATTTTCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTga     <  2:70289/251‑1 (MQ=255)                                                                                                                                                                                                                                          tttCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTgaag   <  2:290417/250‑1 (MQ=255)                                                                                                                                                                                                                                            tCCACGCTG‑CCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCACCCCAGGTGGAAGGGCTTgaaga  >  2:444765/1‑249 (MQ=255)                                                                                                                                                                                                                                                    tgCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTg                                                                                   >  2:146085/2‑161 (MQ=255)                                                                                                                                                                                                                                                    tgCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTg                                                                                   <  1:146085/160‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CGCGGCACGCTGCTGGTTCATGCCGATCTCACCTTTGAAAATCTGTCTCACACCCGGAGCGCGTTATGAAAAAGATCCTGACAACCCCGATCAAAGCTGAAGATCTGCAAGATATTCGCGTCGGCGATGTGATCTACCTGACCGGTACGCTGGTGACCTGCCGCGACGTTTGTC‑ACCGCCGTTTGATCGAACTGAAACGTCCGATCCCTTACGATCTCAACGGCAAAGCGATTTTCCACGCTGGCCCCATCGTGCGCAAAAACGGCGACAAATGGGAGATGGTCTCCGTCGGCCCGACAACCAGTATGCGTATGGAAAGTTTTGAACGTGAATTTATTGAGCAGACCGGCGTGAAACTGGTGGTTGGCAAAGGTGGTATGGGGCCGCTGACCGAAGAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGA  >  NC_000913/3207306‑3207789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑