Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913579,6510CT72.4% 84.4 / 38.6 59intergenic (‑57/‑229)ybcV/ybcWDUF1398 domain‑containing protein YbcV/uncharacterized protein YbcW
Reads supporting (aligned to +/- strand):  ref base C (8/8);  new base T (24/18);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 7.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.10e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAAT  >  NC_000913/579403‑579895
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    
agttACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGGTTTCTAACTCTATGTTAATAATATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCATAATAAACCAATTCAAAGTTTAAATCTTTTCGTTCTTGATCTAATATTCCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGGGATACGAGGGCGCGTAGTTTGCATTATCATTTTTATCGCtt                                                                                                                                                                                                                                                    <  1:315654/249‑1 (MQ=255)
  ttACAACCTGTTTAAGTCCTTTTATTAACCCGGTGTTTTCGTTTTCTAACACAATGTGAATATTATCTGAGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATAGCAACCTGAGCCATTGGAAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTc                                                                                                                                                                                                                                                   >  2:222884/1‑250 (MQ=255)
      aaCCTTTTTAAGTCCTTTTATTACCACGGTGTTATCGTTTTCTAACACGCTGTGAATATTATCTGTGGCTAGATAGGAAATATAATGTGAGACGTTGTGGCGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGAACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAACACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCt                                                                                                                                                                                                                                              >  2:258627/1‑251 (MQ=255)
        ccTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTgg                                                                                                                                                                                                                                            >  2:252108/1‑251 (MQ=255)
          tttttAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTc                                                                                                                                                                                                                                          >  1:10223/1‑251 (MQ=255)
                aGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAATCCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACct                                                                                                                                                                                                                                    <  1:395470/251‑1 (MQ=255)
                aGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATGGTTTTTATGGTTTCAATCTGGTCTgcccc                                                                                                                                                                                                                                    >  2:195444/1‑247 (MQ=255)
                aGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACct                                                                                                                                                                                                                                    >  1:91662/1‑251 (MQ=255)
                         attaACACGGTGTTATCGGTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCGCAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGtt                                                                                                                                                                                                                           <  1:238475/251‑1 (MQ=255)
                          ttaACACGGTGTTATCGTTTTCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGttt                                                                                                                                                                                                                          >  1:133422/1‑251 (MQ=255)
                                 ggTGTTATCGTTTTCTAACACGATGTGAATAGTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTTGTAAATCCTTCCATGTGATACGATGGCGCGTAGTTTGCATTATCGTTTTGAGCGGTTGAATCTGGTCTGAGCTACTTGTGTTTTGTtgat                                                                                                                                                                                                                   <  2:42506/251‑1 (MQ=255)
                                            tttCTAACACGATGTTAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTTACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGTTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTTCATTATCTTTTTTTTCGTTTCAATCTGGTCTGACCGCCTTGTGTGTTTTTGAGGATTTATGTc                                                                                                                                                                                                         <  2:288361/250‑1 (MQ=255)
                                              tCTAACACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCaa                                                                                                                                                                                                       <  1:19334/250‑1 (MQ=255)
                                              tCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCaa                                                                                                                                                                                                       <  1:252108/250‑1 (MQ=255)
                                                 aaCACGATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAatat                                                                                                                                                                                                   >  2:147800/1‑251 (MQ=255)
                                                 aaCACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAatct                                                                                                                                                                                                   >  2:202391/1‑249 (MQ=255)
                                                     cgaTGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAgg                                                                                                                                                                                               <  1:298569/249‑1 (MQ=255)
                                                          tgAATATTATCTATGGCTAGATAGAAAAAATAATGTGAGACGATGTGACGTTTTAGTTCAGAATAAAACAAATCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAAACTGATCCATTGGAAAAACCTTCCATGTGATAAGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGTTTTATGTCAAATATTAGGAATGt                                                                                                                                                                                          >  2:26664/1‑251 (MQ=255)
                                                           gAATATTATCTGTGGCTAGATAGTAAATATAATGGTAGACATTGTGACGTTTTAGTTTAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCTAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAACCCTTCCATGTGATACGAGTGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGTCCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGtt                                                                                                                                                                                         <  1:378487/251‑1 (MQ=255)
                                                           gAATATTATCTGTGGCTAGATAGTAAATAGAATGTGAGACGTTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCTCACTTGATCGAATATTTCTTTAAAAATTGCATCCTGATCTATTGGTAAAGCCTTCCATGTGATACGAGGGTGCGTTGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCGTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGtt                                                                                                                                                                                         <  1:428901/251‑1 (MQ=255)
                                                                              aTAGTACATATAAAGTGAGACGTTGTCACGTTTTCGTTCCGGATAAAACAATTACCAGTCTCAATCTTTTCGCACTTGATAGAATATATCTTTACAAATGGAAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGAATTGTCGTTTTTATCGTTTCAATCTGGTCTGACATCCTTGTGTTTTGTTGTTGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGt                                                                                                                                                                      >  2:61153/1‑251 (MQ=255)
                                                                               tAGTAAATATAATGTGAGACATTGTGACGATTTAGTTCAGAATAACACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAACAATGGCAACCTGAGCCATTGGTAAAAACTTCCATGTGATACAAGGGAGCGTAGTTTGCATTATCATTTTTATCGCTTCACTCTGGTCTGACCTCTTTGTGTTTTGTTTATGATTTATGTAAAATATTAGGATAGTTTTCATTTATTAGTATTGGtt                                                                                                                                                                     >  2:411786/1‑251 (MQ=255)
                                                                                aGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCGGAGCCATTGGTAAAACCTTCCATGTGATACGTGGTCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGTTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGtt                                                                                                                                                                     <  1:69206/250‑1 (MQ=255)
                                                                                   aaaTATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCg                                                                                                                                                                  <  2:71358/250‑1 (MQ=255)
                                                                                      tataATGTGAGACGTTGTGACGGTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTAGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTATTAGTATTGGTTGCGTAAc                                                                                                                                                              >  1:17301/1‑251 (MQ=255)
                                                                                         aaagtgAGACGTTGGGACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGAAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACaa                                                                                                                                                            >  2:76200/4‑250 (MQ=255)
                                                                                          aTGTGATACATGGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAg                                                                                                                                                          <  2:109997/251‑1 (MQ=255)
                                                                                              gagacgctgtgACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCg                                                                                                                                                      >  1:117584/8‑251 (MQ=255)
                                                                                                    ttGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCctg                                                                                                                                                >  1:230155/1‑251 (MQ=255)
                                                                                                      gtgACGTTTTAGTTCAGAATAAAACAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCctgct                                                                                                                                              >  1:274739/1‑251 (MQ=255)
                                                                                                          cGTTTTCGTCCAGAATAAAACAATTCACAGTCTAACTCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAAACTGAGCCATTGGTAAACCCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGc                                                                                                                                           >  2:304009/1‑250 (MQ=255)
                                                                                                                          aaaaCCATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATAc                                                                                                                          >  1:348444/1‑251 (MQ=255)
                                                                                                                          aaaaCAATTCACAGTCTAAATCTTTGCGCACTTGATCGAAGATTTCTTTAAATATGGCAACCTGAGCCATTGGTAAATCCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTTTCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATAGTAGGAAGGTTTTCACTTAATAGTATTGGTTGCGTATCAAAGTTCGGTCGTGCTGGCATTCTGGTGGGAAATa                                                                                                                           <  2:260894/250‑1 (MQ=255)
                                                                                                                             aCAATTCACAGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAAc                                                                                                                       <  1:297143/251‑1 (MQ=255)
                                                                                                                                    acaGTCTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAc                                                                                                                   >  1:362960/1‑248 (MQ=255)
                                                                                                                                           aaaTCGTTTCGAACTTGATCGAATATTTCTTGAAAAATGGCAACCTGAGCCATTGGTATAATCTTCCATGTGATACGAGGGCGCGTAGTTTTCATTATCGTTTTTATCGTTTCAATCTGGTCTGATCTCCTTGTTTTTTGTTGTTGATTTATGTCAAATATTAGGAATTTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGTAAATTCAACCGCCAGatgta                                                                                                            <  2:244985/248‑1 (MQ=255)
                                                                                                                                                 tttCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAg                                                                                                      <  2:18358/248‑1 (MQ=255)
                                                                                                                                                 tttCGCACTTGAAAGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATAAGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGACTGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGcc                                                                                                   >  2:386831/1‑251 (MQ=255)
                                                                                                                                                  ttCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGcc                                                                                                   <  1:269120/250‑1 (MQ=255)
                                                                                                                                                      cACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGt                                                                                              <  1:158714/251‑1 (MQ=255)
                                                                                                                                                              cGAATATTTCTTTAAAAATGGCACACTGAGCCATTGGTAAAACCTTCTATGTGATAAGAGGGCGCGTAGTTTGAATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGCTGATTTATGTCACATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCaaat                                                                                      >  2:454345/1‑250 (MQ=255)
                                                                                                                                                              cGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTTATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGCTGTATGTAAGGACAACGTGCTCAAAc                                                                                      >  2:106928/1‑251 (MQ=255)
                                                                                                                                                              cGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTAGTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCaaa                                                                                       <  2:126713/250‑1 (MQ=255)
                                                                                                                                                                     ttCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCAt                                                                                >  1:6835/1‑250 (MQ=255)
                                                                                                                                                                        tttAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGAAAGATGTATGAAAGGCCAACGTGCTCAAATCTTCATAc                                                                              >  1:1781/1‑249 (MQ=255)
                                                                                                                                                                          tAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGa                                                                           <  1:144877/250‑1 (MQ=255)
                                                                                                                                                                            aaaaTGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGAGTAGTATACATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAAAATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGaa                                                                          >  1:133487/1‑249 (MQ=255)
                                                                                                                                                                              aaTGGCAACCTGAGACATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGaa                                                                          >  2:78235/1‑247 (MQ=255)
                                                                                                                                                                                     aCCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGt                                                               <  1:195083/251‑1 (MQ=255)
                                                                                                                                                                                       cTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTa                                                              >  1:246250/1‑250 (MQ=255)
                                                                                                                                                                                              aTTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTa                                                      <  2:362960/251‑1 (MQ=255)
                                                                                                                                                                                              aTTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTa                                                      <  1:63408/251‑1 (MQ=255)
                                                                                                                                                                                               ttGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTACTATTTTa                                                      >  2:232167/1‑250 (MQ=255)
                                                                                                                                                                                                                           gcgcGTAGGTTGCATTATCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGATTGTTTTCACTTAATAGTATTGGTGGCGTAACAAAGGGCGGCCCTGCTGTCATTCTAGAGGGAAATACAGCCTACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATATAGAAATATTTGAATTGATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAg                         <  1:215413/251‑1 (MQ=255)
                                                                                                                                                                                                                               gTAGTTTGCATTATCGTTTTTATCGTTTAACTCTAGTCTGACCTCCTTGTGTTTTGTTGTTAATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGTTTGCGTAACAAAGTGCGGTACTACTGGCATTCTGGAGGGAAATACAACCGACAGATGAAAGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGTTTTGAAGTAATATTTTAACCGCTAGATGCAGAGCAAGCGCATGGAGCgc                      >  2:268610/1‑249 (MQ=255)
                                                                                                                                                                                                                                     ttccttttCGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAAt                <  1:195444/243‑1 (MQ=255)
                                                                                                                                                                                                                                     tGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACAGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGCTTTGAAGTAATATTTTAACCGATAGATGAAGAGCAAGCGCATGGAGCGACAAAATg               >  1:245650/1‑251 (MQ=255)
                                                                                                                                                                                                                                             cGTTTTTATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATaa          >  1:108599/1‑248 (MQ=255)
                                                                                                                                                                                                                                                   tATCGTTTCAATCTGGTCTGACCTCCTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCACTTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAATCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAAt  <  1:67232/250‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    
ATTTACAACCTTTTTAAGTCCTTTTATTAACACGGTGTTATCGTTTTCTAACACAATGTGAATATTATCTGTGGCTAGATAGTAAATATAATGTGAGACATTGTGACGTTTTAGTTCAGAATAAAACAATTCACAGTTTAAATCTTTTCGCACTTGATCGAATATTTCTTTAAAAATGGCAACCTGAGCCATTGGTAAAACCTTCCATGTGATACGAGGGCGCGTAGTTTGCATTATCGTTTTTATCGCTTCAATCTGGTCTGACCTCTTTGTGTTTTGTTGATGATTTATGTCAAATATTAGGAATGTTTTCAATTAATAGTATTGGTTGCGTAACAAAGTGCGGTCCTGCTGGCATTCTGGAGGGAAATACAACCGACAGATGTATGTAAGGCCAACGTGCTCAAACCTTCATACAGAAAGATTTGAAGTAATATTTTAACCGCTAGATGAAGAGCAAGCGCATGGAGCGACAAAATGAATAAAGAACAAT  >  NC_000913/579403‑579895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑