Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,361,732	IS5 (–) +4 bp	coding (557‑560/765 nt)	gltF →	periplasmic protein GltF

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3361732 =	0 (0.000)	44 (1.080)	42/496	0.1	100%	coding (557/765 nt)	gltF	periplasmic protein GltF
	?	NC_000913	= 3366750	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 3361735	0 (0.000)	44 (1.080)	43/496	0.1	100%	coding (560/765 nt)	gltF	periplasmic protein GltF
	?	NC_000913	3365556 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

GTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/3365308‑3365799                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    gttgccctgggTAAAACATCACAATTAGCAGACAGAACAAGCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATAATTCCAACAACGTATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCTCAGGAACAGCATCAATTAGGGa                                                                                                                                                                                                                                                   <  1:343235/243‑1 (MQ=255)             aaaaCAACACAATTAGCAGACAACACAATCCCGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTAAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTCGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATa                                                                                                                                                                                                                                        >  2:43094/1‑250 (MQ=255)                           aGCAGACAGAAAACTCAAGCTGGTACTGCAATGCGATCAGGACGCTCATATTCATGTTCAGTTGAATACCGCTAAAATTTCCAACAACGGATTTGGACTTAGTCCGAATGGTTCATTAAATCTGATCGCCAGCGATACCTTTTCAGGTAGTAACAATCTAGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGAAGCCCAGGAACAGCATCAATTAGGGAAGGAGCGACTAGGCGGGGAAAttctt                                                                                                                                                                                                                         >  2:220131/1‑251 (MQ=255)                                          cACGCTGGTAATTCAATGCGATCAGGACGCTAATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGCTTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTGGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGTTGCGAATAAGCGGGGATATTCTTCTCGGCTGACTCAg                                                                                                                                                                                                           <  2:318519/250‑1 (MQ=255)                                          cACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAACGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAg                                                                                                                                                                                                           <  2:9234/250‑1 (MQ=255)                                             gCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcat                                                                                                                                                                                                       <  1:414933/251‑1 (MQ=255)                                                   aatgcaatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGACAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcatt                                                                                                                                                                                                 >  1:230777/1‑251 (MQ=255)                                                     tgcaatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                                                                                                                               >  1:403181/1‑251 (MQ=255)                                                       caatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                                                                                                                             >  1:447093/1‑251 (MQ=255)                                                           gcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGAGTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATg                                                                                                                                                                                         <  2:188681/251‑1 (MQ=255)                                                                cAGGACGATCATATTCGAGTTCCGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGAACAGGAACAGCATCGATTAGGGGAGGAGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGCCATTTAATTTCTTCATGTTTgg                                                                                                                                                                                    >  1:102427/1‑250 (MQ=255)                                                                   gACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc                                                                                                                                                                                 <  1:334881/251‑1 (MQ=255)                                                                    aCGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCg                                                                                                                                                                                >  1:413023/1‑251 (MQ=255)                                                                     cGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCg                                                                                                                                                                                >  2:92798/1‑250 (MQ=255)                                                                         cATATTCGTGTGCAGTTGAATACCGCTAATATGTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTTATCGCCATCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCAGCAATTAGGGAAGGTGCGAATAAGGTGGGAAAGTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtt                                                                                                                                                                             <  1:246882/249‑1 (MQ=38)                                                                                          gAATACCGCTCATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTAGACCTGGCGTTAGCCAGTGGCAACAATGACAATCCGGGCCGCACAGGAACAGCCTCAATTAGGGAAGGTGCTAATACGCGGGGAAATTCTTCTCGGCTGACTCATTCATTTCATTTCTTCCTGTTTGCGCCGATTTTTTCTCCCGTCAATGCCt                                                                                                                                                          >  2:169405/1‑251 (MQ=255)                                                                                          gAATACCGCTAATATTTCCATCTACGGATTTGGTTTTGTCCTGAATGGTTCATTTAATCTGATCGCCAGCGATGCATTTTCATGTATTAACAAACTCTACCTGGCGTTAGCCAGTGGCATAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAATGTGCGAATATGCGGGGAAATTCTTCTCTGCTGACTCAGTTATTTCATTTCTGCATTTTTGAGCCGATTTTTTCTCCCGTAAATGca                                                                                                                                                           <  2:101796/250‑2 (MQ=255)                                                                                              aCCGCTAATATTTCCAACAACGGATTTGGATTTTGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGaa                                                                                                                                                      <  2:75209/251‑1 (MQ=255)                                                                                                 gCTAAAATTTCAACCACCGGATTTGGATTTGGCCCGAATGGGTCATTAACTCTGATCGCCAGCGATGCATTTTCAGGTAGTACCAATCTCTACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGACCAGCATCAATTAGGGAAGGAGCGAATAAGCGGGCACATTCTTCTCGGCTGCCTCAGTCATTTCATTTCTTCATGTTTTCGCCGGTTTTTTCTCCCGTACATTCCTTGAATc                                                                                                                                                    >  2:299860/1‑250 (MQ=255)                                                                                                    aaTATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCCTTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGcc                                                                                                                                                >  1:425246/1‑251 (MQ=255)                                                                                                            caacaaCGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGACAGTGGCAAAAATGACAATCCGGGCAGCACAGGAAAAGCATCAATTAGGGAAGGTGAGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTAATTTCTTCATGTTTGAGCCGATTTTTTCTCACGTAAATGCCTTGAATCAGCCTAttt                                                                                                                                           >  1:383867/1‑248 (MQ=255)                                                                                                                 acGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGtt                                                                                                                                   <  1:320973/251‑1 (MQ=255)                                                                                                                   ggatttcgatttGGCCCGAATGGTTCATTACATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACACTCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGAACAGGACCAGCATCAATTCGGGAAGGAGCGAATAAGAGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGCGCCGGTTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttc                                                                                                                                 >  2:170128/1‑251 (MQ=25)                                                                                                                         ggatttggCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc                                                                                                                           >  1:199198/1‑251 (MQ=255)                                                                                                                         ggatttggCCCGAACGGTTCATTAAATCTGATCGCCAGCGATGCATTTGCAGGTAGTAACAAACTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCTGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTATCCCGTTTCTTCgac                                                                                                                           <  2:123280/251‑3 (MQ=25)                                                                                                                                                     tGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGt                                                                                               >  1:103709/1‑251 (MQ=255)                                                                                                                                                                               agtaACAATCTAGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGACATTCTTCTCGGCAGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATACCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCAtt                                                                     >  1:85790/1‑251 (MQ=255)                                                                                                                                                                                                  cGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGt                                                  >  2:9901/1‑251 (MQ=255)                                                                                                                                                                                                           gTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCCTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCg                                         >  2:241644/1‑251 (MQ=255)                                                                                                                                                                                                            tGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCgg                                        >  1:386003/1‑250 (MQ=255)                                                                                                                                                                                                                       aCAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGAGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTAGTCCGCCCGAAACAGGTTGACCAGCGTGACTAACATCg                             >  2:95273/1‑251 (MQ=255)                                                                                                                                                                                                                                  agcaCAGGAACAGCATCCATTAGGGAAGGTGCGAATAAGCGGGGCAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGCTCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGttt                  >  2:357125/1‑250 (MQ=255)                                                                                                                                                                                                                                  agcaCAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGtt                   >  1:180496/1‑250 (MQ=38)                                                                                                                                                                                                                                       tggcacaacATCAATTCGGACAGGTGCGCATAAGCGGGGAAATTATTCTCGGCTGACTAAGTCATTACATTTCTTCGTGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCATATTTAGACCGTTTCTTCGCCATTTAAGACGTTATCCCCAGTTTTTAGTGTGATCTCTACAACTGACGTATCATTTGGTCCGCCCGAACCAGGTTTGCCATCGTGAATTACATCGCCAGTTGGCTATCGtt             >  2:48598/9‑251 (MQ=11)                                                                                                                                                                                                                                        ggAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGGTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGttt            <  2:199198/251‑1 (MQ=25)                                                                                                                                                                                                                                            cAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTc         >  2:442920/1‑250 (MQ=33)                                                                                                                                                                                                                                                  cAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAc   <  1:92798/250‑1 (MQ=31)                                                                                                                                                                                                                                                  cAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAc   <  2:447093/250‑1 (MQ=31)                                                                                                                                                                                                                                                  cAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCATTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAcc  >  2:191383/1‑251 (MQ=14)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    GTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGATTTGGCCCGAATGGTTCATTAAATCTGATCGCCAGCGATGCATTTTCAGGTAGTAACAATCTCGACCTGGCGTTAGCCAGTGGCAAAAATGACAATCCGGGCAGCACAGGAACAGCATCAATTAGGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/3365308‑3365799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.