Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,671,143	IS2 (+) +5 bp	intergenic (+191/‑229)	ynfM → / → asr	putative transporter YnfM/periplasmic chaperone Asr

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1467910 =	NA (NA)	22 (0.540)	20/496	0.7	95.6%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	1671143 =	1 (0.030)					intergenic (+191/‑233)	ynfM/asr	putative transporter YnfM/periplasmic chaperone Asr
*	?	NC_000913	= 1671147	1 (0.030)	28 (0.690)	24/496	0.5	96.5%	intergenic (+195/‑229)	ynfM/asr	putative transporter YnfM/periplasmic chaperone Asr
	?	NC_000913	= 2070271	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

TGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATACAACTTTTTA  >  NC_000913/2070026‑2070516                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      tGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTgg                                                                                                                                                                                                                                                   <  1:89648/250‑1 (MQ=11)  gCTTACGCTAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTGAAGTGATATTGGTTGTCTGGAGAGTCAGGGGGCCAGTCTAGTGGCg                                                                                                                                                                                                                                                 <  2:301940/251‑1 (MQ=14)                                                                   aTCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCAGCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGAt                                                                                                                                                                                <  1:276151/251‑1 (MQ=255)                                                                          gTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATg                                                                                                                                                                         <  1:238711/251‑1 (MQ=255)                                                                            tCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGCCATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGAt                                                                                                                                                                                                                 >  2:246669/1‑209 (MQ=255)                                                                            tCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGAt                                                                                                                                                                                                                 <  1:246669/209‑1 (MQ=255)                                                                             cAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGgtg                                                                                                                                                                      >  1:86672/1‑251 (MQ=255)                                                                               aaGCTCTGCTCAACAATTGCGATCTTTTCCTGTGTGGTACGCAGCCTGCGTTTCTCCGGCCCTAAGACATAAATCATCTGTTCTCCACTGACTAGTCTAAAACCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTACTTCTCATTGATGGTCAGGAGGTAACTTATCAGAGCGCCGGAGAAAATTATGGtgtg                                                                                                                                                                    >  2:165035/1‑251 (MQ=18)                                                                                                                                gTTTCTCAGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCCGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGaa                                                                                                                     >  2:211078/1‑249 (MQ=255)                                                                                                                                        ggCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTACAAACTCGAATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGAGAAGCATCCTCCCGTGTTTTAATTCTCATTGCTGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCatat                                                                                                           >  1:264727/1‑251 (MQ=255)                                                                                                                                        ggCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTGTGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCatat                                                                                                           <  1:420304/251‑1 (MQ=255)                                                                                                                                          cccTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTc                                                                                                         <  1:89802/251‑1 (MQ=255)                                                                                                                                            cTAAGACATCAATCCTCTGTTCACCAATGACTAGACTACAAACTAGCATTAAGACTCTCACTTATTTACGTGATATTGGTTGTCTAGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCCGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCg                                                                                                       >  1:137019/1‑251 (MQ=255)                                                                                                                                              aagtcATCAAACATCTGTGCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGGCTGGAGATTCAGGGTGCCAGTCTAGTGTCGAAGCATCCTCCCGTTTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATATATGATTCTGCTCTGGAGCGTGTTGAGCTAGTTAAAGTTCCATATTCCg                                                                                                       <  2:405116/245‑1 (MQ=25)                                                                                                                                                           tcTGTTCTCCAATGAATAGTCTAAAAACTAGTCTTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGAATCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTATCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGGGTATGCGCTGGAGCGTGTTGAGGGAGGTAAAGGTTCATAGTCTGTACTGTACGGTTcac                                                                                        >  2:289728/1‑251 (MQ=37)                                                                                                                                                              gTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTcaca                                                                                       <  1:108334/249‑1 (MQ=255)                                                                                                                                                                        gACTAGTCTAAAAACTAGTATTAAGACTATCTCTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGAGGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAATGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCgg                                                                           <  1:171410/251‑1 (MQ=255)                                                                                                                                                                        gACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCgg                                                                           <  1:57101/251‑1 (MQ=255)                                                                                                                                                                                 aaaaaCTAGTATTAAGTCTATCTCGTATTTAAGTGATATTGGTTGGCTGGAGATTAAGGGGTCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTATCTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGta                                                                    <  2:112467/249‑2 (MQ=255)                                                                                                                                                                                                ggcTATCACTTATGTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGGGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGTAGCGTGTTGAGGTAGTGAAATGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCaaaaa                                                   <  1:210709/249‑1 (MQ=255)                                                                                                                                                                                                 aCTATCACTTATTTAAGTGATATTGGTTGTCTGCAGATTCAGGGGGCACGACTAGAGGCGAAGCATCCTCCCGTGTTTTAACTCCCATTGATGGTCAGGAGGTAAATTATCCGCGCGCCGGAGATCATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGATCACAGGCAATTGGCGGTATAGTTAAATtcaaaacccaca                                                    >  1:248979/1‑240 (MQ=255)                                                                                                                                                                                                       aCTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGTGCCAGTCTAGTGGCGAAGCATCCGCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAACTCGCCGTAGATAATTATGGTGTGGTACCGTTGATAGATGAGTCTGCGCTGGAGGGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTGCACAGGCAATTTGCGGTATTGTTAACGTCATCCCCAAAAAGGGTGaa                                            <  2:101503/251‑1 (MQ=255)                                                                                                                                                                                                          tATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCATCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGCTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCCTCACCAAAAAGGGTGAAtct                                         >  1:393702/1‑251 (MQ=255)                                                                                                                                                                                                            tttAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTcc                                       >  1:148996/1‑251 (MQ=255)                                                                                                                                                                                                              tAAGTGATATTGGTTGTCTTGAGATTCAGTGGGCCAGTCTAGTGGCGAAGCATCCCCCCGTGTTTTAATTCTCATTGATGTTCAGGATGTAACTTATCAGCGCGCCGGAGATAATTATGTTGTGGGACTGTTGTTAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGTCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCg                                      <  1:193252/250‑1 (MQ=255)                                                                                                                                                                                                                 gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGCGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTAAGGCGGTAACTTATCAGCGCGCCGGCGATAATTATGGTGTGGGACTGTTGATAGATGCGTCTGCGCTGGAGCGTGTTGAGGTAGTGAACGGTCCATATTACGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTACGGAcc                                  >  1:247341/1‑250 (MQ=255)                                                                                                                                                                                                                        tGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCtat                            <  1:98279/250‑1 (MQ=255)                                                                                                                                                                                                                         ggTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTCATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGCGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGGGAATCTCCGGACTCCCTatat                          >  2:375672/1‑251 (MQ=255)                                                                                                                                                                                                                             gtctggcaatccAGGGGGCAAGTCTTGTGGCAAAGCATCCTCCCGCGTTTTAATTCTCCTTACTGGCTAGGAGGAAACCTATCAGCGCGACGGAGATAATTATGGTATGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGGAGTGAAAGGTCAATATTCCGTACTGTACGGTTAACAGGCAATTGGCGGTATTGTTAAATTCATCCACAAAAAGGGGCAATCACAGGAATCCCTatattcct                      >  2:418733/12‑247 (MQ=255)                                                                                                                                                                                                                                 ggAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTaa                   <  1:165035/250‑1 (MQ=255)                                                                                                                                                                                                                                                cAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATACAACttttt   >  1:47175/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATACAACTTTTTa  <  2:95605/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      TGCTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAGTGGCGAAGCATCCTCCCGTGTTTTAATTCTCATTGATGGTCAGGAGGTAACTTATCAGCGCGCCGGAGATAATTATGGTGTGGGACTGTTGATAGATGAGTCTGCGCTGGAGCGTGTTGAGGTAGTGAAAGGTCCATATTCCGTACTGTACGGTTCACAGGCAATTGGCGGTATTGTTAACTTCATCACCAAAAAGGGTGAATCTCCGGACTCCCTATATCACTTAAATTGATACAACTTTTTA  >  NC_000913/2070026‑2070516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.