Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	926,188	IS2 (–) +5 bp	intergenic (‑217/+33)	serW ← / ← infA	tRNA‑Ser/translation initiation factor IF‑1

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	926188 =	0 (0.000)	20 (0.500)	18/492	0.8	100%	intergenic (‑217/+37)	serW/infA	tRNA‑Ser/translation initiation factor IF‑1
	?	NC_000913	= 1469240	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 926192	0 (0.000)	27 (0.670)	27/494	0.4	100%	intergenic (‑221/+33)	serW/infA	tRNA‑Ser/translation initiation factor IF‑1
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

AACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATAC  >  NC_000913/1469013‑1469488                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         aaCTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTg                                                                                                                                                                                                                                   >  1:62902/1‑251 (MQ=38)  tctggctggCTGATACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTCTTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGCACTTACTTATGCTg                                                                                                                                                                                                                                   >  2:115308/2‑250 (MQ=255)                             gggCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAgg                                                                                                                                                                                                       <  1:187177/251‑1 (MQ=255)                                    gAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGt                                                                                                                                                                                                <  2:237125/251‑1 (MQ=255)                                                               tctctGCTGAACAATAGCGATCCTTTGCTGTGTGGAACGACGACTGCGTTTCTCTGGCCCTAAGAAATAAATCATCTGTTATCCAATGCCTAGTCTCAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCTGTCTAAAACCACGAACTTACTTATGCTGATTACGGAGCGAAAATTGTCAATAAAGGAACTTGTAGCTTCAATTGGTCAGCGCAACAGtt                                                                                                                                                                      >  2:273089/2‑250 (MQ=17)                                                                                       ttCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAACCCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGCTTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAgg                                                                                                                                             >  2:18144/1‑251 (MQ=255)                                                                                            gtgtgGTACGCCGTCTGCGGTCAGGCGGCCCTAAGACATCAATCATCTGTTCTCCAATGTCTAGTCTAGAAACTAGTATTAAGACTGTCACTTAGTTAAGGGAGGTTAGTTGTCTTGAGATGCAGGGGGCCAGTCTAAATCCCAGAACTAGCTTATGCTGATTGCGTTTCGAAAATTTTTAATAAAGATACTTGGAGATTCAATTGGTCAACGCATCACCTATGTGAAAACATGGGGTTGCGGATGttttt                                                                                                                                        <  2:222741/251‑1 (MQ=16)                                                                                                ggTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGa                                                                                                                                     >  1:57927/1‑250 (MQ=255)                                                                                                                                   caatcaTCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCctct                                                                                                 >  1:69141/1‑251 (MQ=255)                                                                                                                                               ttcCAATGACTAGTCTAAAAACTAGTATTAAGACGATCACTTATTTAAGTGATATTGGTTGTCTGGAGATGCAGGGGGCCAGTCGAAACCCCAGAACTTACGTATGCTGATTCCGTTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGGTTTTTGAATGAGACGAACAGTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACt                                                                                      <  2:290264/249‑1 (MQ=37)                                                                                                                                                 ccAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTAt                                                                                    <  1:338327/250‑1 (MQ=255)                                                                                                                                                                                  aTCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGAGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGa                                                   >  1:264013/1‑250 (MQ=255)                                                                                                                                                                                                    atTGGTTGTCTGGAGAGTCAGGGGTCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGGCAACGCAACAGATATGTTAAAACATGGGGTTACGGAGGTTTTTTTAATGAGACGAACATTTTCAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACCGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCataaa                                 <  2:361387/250‑5 (MQ=21)                                                                                                                                                                                                         ttGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGa                            >  1:111154/1‑250 (MQ=255)                                                                                                                                                                                                           gTCTGGAGATTCAGGGGGCCAGACTAAACTCCAGAACGTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATGCAATTGGTCAACGCAACAGGTATGTGAAAACATGGGGTCGGGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAACAGCCTCTGTTGTTGAACTATGGAAGAACGGAACAGGGTTCAGTGAAATAGCGAATATGCTGGGTACAAAACCCGTAAc                          <  2:253290/250‑1 (MQ=18)                                                                                                                                                                                                             cTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAAc                                                                            >  1:275153/1‑198 (MQ=255)                                                                                                                                                                                                             cTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTAGGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCGCTGTTTTTGAACTATGGAAGAAc                                                                            <  2:275153/198‑1 (MQ=255)                                                                                                                                                                                                               ggAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATc                      <  1:172846/250‑1 (MQ=255)                                                                                                                                                                                                                 agaTTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTCTGCTGCTTCCGGTGCGAAACTTGATCATAGAAGTCATTGAAGATTCAATTGGTCAACACAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGGGGAATAAGCCTCTGTTTTTGAACTCTGGAAGAACGGCACAGGATTCCGTGAATTAGCGAATATCATGGGTTCAAAGACCGGAAAGATATTc                   >  2:372152/1‑251 (MQ=14)                                                                                                                                                                                                                    ttCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACt                >  1:161014/1‑251 (MQ=255)                                                                                                                                                                                                                     tCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACt                >  1:351412/1‑250 (MQ=255)                                                                                                                                                                                                                                  cTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATAc  >  1:275958/1‑251 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                         AACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATAC  >  NC_000913/1469013‑1469488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.