Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	363,261	G→T	Y1015* (TAC→TAA)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	363,261	0	G	T	98.7%	246.4 / ‑5.9	78	Y1015* (TAC→TAA)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (47/30);  total (47/31)
Fisher's exact test for biased strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

AGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTC  >  NC_000913/363019‑363503                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   agagAAATAGCGACAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAACAGTAAAAGAAAAAGAATAAACCGAAAATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGAcc                                                                                                                                                                                                                                            >  2:132918/1‑251 (MQ=255)   agaAATAGCGGCAAAACTAATACTCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTACATAGTACATAATGGATTTCCTTACACGAAATACGGGCAGACATGGCCTGCCCGGTTTTTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCgg                                                                                                                                                                                                                                          >  2:87953/1‑251 (MQ=255)    gaAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTTTTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGgc                                                                                                                                                                                                                                         >  1:303037/1‑251 (MQ=255)         aGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATGATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCa                                                                                                                                                                                                                                    <  1:431327/251‑1 (MQ=255)                  aataatACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAAtt                                                                                                                                                                                                                           >  1:327422/1‑251 (MQ=255)                            gTATCTCTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAACCGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAACCCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGTATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGTTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGCCCGGCGCTCAGCTGTAATTCCGCCGATAc                                                                                                                                                                                                                 <  1:123592/251‑1 (MQ=255)                                  cTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACgg                                                                                                                                                                                                           >  1:436299/1‑251 (MQ=255)                                      tGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTc                                                                                                                                                                                                       >  2:174084/1‑251 (MQ=255)                                             atGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAgg                                                                                                                                                                                                >  2:5625/1‑250 (MQ=255)                                               ggTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAgtc                                                                                                                                                                                               <  1:165259/250‑1 (MQ=255)                                                gTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAACGTTTGTGTTTTTTAAATAGAACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCCCCAGGAgtcgt                                                                                                                                                                                             >  2:383819/1‑251 (MQ=255)                                                   gATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGc                                                                                                                                                                                          >  1:240561/1‑251 (MQ=255)                                                                         aaaaaCGGAAAGTAGGCTCCAATGATAAAAAAGTAAAAGAAAAAGAATACACCGAACATCAAAAAGTTTGTGTTTTATAAATAGTACATAATGTATTGCCTTGCGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACTAACTGGTAATGTTAGCGACCGGCGCTCACCTGAAACTCCGCCGATACTGCCGGGCTCCAGGAGTCGGCGCCACCAATCCCCATATGTAAAcc                                                                                                                                                                    <  2:30775/251‑1 (MQ=255)                                                                            aaCGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGGCGTCGCCACCAATCCCCATATGGAAACCGTc                                                                                                                                                                 >  2:16599/1‑251 (MQ=255)                                                                               gggACGTATGCTCCCATGTTAAAAAAGTAAAAGAAAAAGAATAAACCGAACACCCAAAAGTTTGTGTGTTTTAAATAGTCCATAATGGATTTCCTTACGCGAAATACGGCCAGACAGGGCTTGCCCGGTTATCATTATTTCTGACACCAGACCACCTGGTAATTTTAGCGACCGGCGCTCAGCTGCAATTCCGCCGATACTGACGTGTTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGat                                                                                                                                                              <  2:172812/251‑1 (MQ=255)                                                                                   aGTAGGATCCCACGATCAAAAAGTAAAAGAAAAAGAATAAACCGACCATCCACAAGATTGTGCTTTTTAAATAGTAAATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCACCTGGTACTGTTAGCGACCGGCGCTCAGATGTAATTCCGCCGATCCTGACGGGCTCCAGGAGCCGTCGCCACCAATCCACATATGGAAACCGTCGATATTc                                                                                                                                                          >  2:427100/1‑251 (MQ=255)                                                                                    gTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCa                                                                                                                                                         <  1:16599/251‑1 (MQ=255)                                                                                      aGGCTCCCATTATAAAAAAGTAAAAGAAAAAGAATAACCCGAACATCCAAAAGTTGGTGTTTTTTAAATAGTACATAATGGATTTCCTTAAGCGAAATACGGGCAGACATGGTCTGCCCGGTTATTATTAGTTTTGACACCAGCCCACCTGGTAATGTTAGCTACCGGCGCTCAGCGGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGTAAACCGTCGATATTCAg                                                                                                                                                        <  2:423871/250‑1 (MQ=255)                                                                                              aTGATACAAAAGTAAAAGAAAAAGACTCACCCGAACATCCAAAAGTTTGTGTTTTTTACATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCCACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGcc                                                                                                                                               >  2:416591/1‑251 (MQ=255)                                                                                                gATAAAAAAGTAAAAGAAAAAGAAAAAACCGAACAACCAAAAGTTTGGGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATTTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCctt                                                                                                                                             <  2:394758/251‑1 (MQ=255)                                                                                                   aaaaaaGTAAAAGAAAAAGAAGAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGTATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCGCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCcttctt                                                                                                                                          <  2:7535/251‑1 (MQ=255)                                                                                                    aaaaaGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCttcttc                                                                                                                                         >  1:437026/1‑251 (MQ=255)                                                                                                     aaaaGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTAAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTcc                                                                                                                                        >  1:18020/1‑251 (MQ=255)                                                                                                      aaaGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATCATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCACCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCcg                                                                                                                                       >  2:266876/1‑251 (MQ=255)                                                                                                        aGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCcgcg                                                                                                                                     >  1:264716/1‑251 (MQ=255)                                                                                                           aaaagaaaaagcATAAACCGAAAATCCATAAGGTTGTGATTTTTAAATAGAACATAATGGATTACCTTACGCGAAATACGGGCAGACATGGCCAGCCCGGTTATTATTATTTTTGACACCAGACCAACTGTGAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGAAGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACAGTCGATATTCAGCCATGTGCCTTCTTCCGCGTgc                                                                                                                                  >  2:326285/1‑251 (MQ=255)                                                                                                           aaaagaaaaagaATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCACTCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCcgcgggc                                                                                                                                  >  2:326307/1‑248 (MQ=255)                                                                                                            aaagaaacagaaTAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTgc                                                                                                                                  >  1:111477/1‑250 (MQ=255)                                                                                                            aaaaaaaaagaaTAATCCGAACATCCAAAGGTTTGTTTTTTTTAAATAGTACATATTGGATTGCCTCTCGCGAAATACGGGCATACATGGCCTGCCCTGTTATTATTATTTTTGACACCAGACCAACTTGTAATTTTAGCGACCGGCGCTCGTCTGGAAGTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATTGAAACCGTCGATATGCAGCCATGTGCGTTCTTCCGCGTgca                                                                                                                                 <  2:281880/247‑1 (MQ=255)                                                                                                             aagaaaaagaaTAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTgacc                                                                                                                                >  2:187126/1‑248 (MQ=255)                                                                                                                  aaagaaTAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTgcagc                                                                                                                               >  1:429338/1‑247 (MQ=255)                                                                                                                    agaaTAAACCGAACATCAAAAAGTTTGTGTTTTTTAAATAGTACAAAATGGATTTCCTTACGCTAAATACGGGCAGACATGGCCTGCCTGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAgatgg                                                                                                                         <  2:248219/251‑1 (MQ=255)                                                                                                                     aaaTAAACCGAACATCCAAAAGTTTGTGTTTTTGAAATAGTACATAAAGGATTGCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAGTTTAATCGACCGGCGCTGAGCTGTAATGCCGCCGATACTGACGGGCTCCAGGAGTCGACTCCACCAATCCCCATATGGAAACCGTCGATATTCAGTCATGTGCCTTCTTCCGCGTGCAGCAgatggc                                                                                                                        <  1:252834/250‑1 (MQ=255)                                                                                                                       aTAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAgatggcg                                                                                                                       >  1:28055/1‑250 (MQ=255)                                                                                                                         aaaCCGAACATCCAAAAGTTTGTGGTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCTGCGCTCAGCTGTAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAgatggcgat                                                                                                                     <  1:281467/250‑1 (MQ=255)                                                                                                                           aCCGATCATCCAAAAGTTTGTGTTTTTGAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCTTGCCGGGTTATTATTATTTTTGACACCAGACCAACTTGGAATGTTAGCGACCGGCGCGCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTTCAGCAtttggtgatggc                                                                                                                  <  2:58750/251‑1 (MQ=255)                                                                                                                               aaCATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGt                                                                                                              <  1:349560/251‑1 (MQ=255)                                                                                                                                    ccAAAAGGTGGTGGTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACAGGGCCTGCCCGGATATTATTATTTTTGACACCAGACCAACGGGTAATGTTAGCGCCCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGGCGATATTCAGCCATGGGCCTTCTTCCGCGTGCAGCAGATGGCGAGGGCTGGTTTCCa                                                                                                         <  2:219608/251‑1 (MQ=255)                                                                                                                                    acAAAAGTTTGGGTTTTTTACATAGTACATCATGGATTTACTTAAGCGAAATACGGGCAGCCATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAAATGGTAATGTTAGCGACCTGCGCTCAGCAGGAATTACGCCGATACTGACGGGCTCCAGGAATCGTCGCCCCCAATTCCCATATGGAAACCGTCGCTATTCGGCCATTTGCCTTCTTCCGCCAGTAGCAgatgcctatgtctagattccc                                                                                                         >  2:406151/2‑234 (MQ=255)                                                                                                                                         aGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCAGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGt                                                                                                    >  1:162201/1‑251 (MQ=255)                                                                                                                                            ttGTGTTTTTTAAATAGTACATAATGGCTTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTCGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAgatgg                                                                                                                         >  2:354559/1‑227 (MQ=255)                                                                                                                                            ttGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAgatgg                                                                                                                         <  1:354559/227‑1 (MQ=255)                                                                                                                                                        aaTAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGATATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAg                                                                                     <  1:39546/251‑1 (MQ=255)                                                                                                                                                                  aaTGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGt                                                                           <  1:266876/251‑1 (MQ=255)                                                                                                                                                                   aTGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACAAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGtt                                                                          >  1:157660/1‑251 (MQ=255)                                                                                                                                                                      gATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGa                                                                        >  1:442809/1‑250 (MQ=255)                                                                                                                                                                         ttCCTTTCGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTg                                                                    >  1:130432/1‑251 (MQ=255)                                                                                                                                                                          tCCTTACGCGAAATACGGGCAAACATGGCCTGCCCGGTTATTATTTGTTTTGACACCAGCCCAACTGGTAATGTTAGCGCCAGGCGCCCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCATTTGCTGTTGACTGTAGCGGCTGATGTTGAACTg                                                                    <  2:78959/250‑1 (MQ=255)                                                                                                                                                                            cTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCCGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGa                                                                  >  1:452355/1‑250 (MQ=255)                                                                                                                                                                            cTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGa                                                                  >  1:251922/1‑250 (MQ=255)                                                                                                                                                                            cTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACAAACTGGTCATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGCGTCGTCGCCACCAATCCCCATATGGACACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTggg                                                                  >  2:252719/1‑249 (MQ=255)                                                                                                                                                                                  ggAAATACGGGTAGACATGGCCTGCTGGGTTATTATTATTTTTGACACCAGACCAACTGTTAATGTTAGCGACCTGCGTTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCAGGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGGTGACTGTAGCGGCTGATGTTGAACTGGAAGTcgcc                                                           <  2:315374/250‑1 (MQ=255)                                                                                                                                                                                    aaaTACGGGCAGACATGGCCTGCCCGGTTATTATTCTTTTTGACACCAGACCAACTGGTACTGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGCAACCGTCGATATTCCGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGCCTGTAGCGGCTGCTGTTGAACTGGAAGTcgccgc                                                         >  2:387914/1‑251 (MQ=255)                                                                                                                                                                                     aaTACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGCCCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAg                                                                                                                                                                                                    >  1:157177/1‑111 (MQ=255)                                                                                                                                                                                     aaTACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATTTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAgat                                                                                                                           <  1:358907/184‑1 (MQ=255)                                                                                                                                                                                     aaTACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAgat                                                                                                                           >  2:358907/1‑184 (MQ=255)                                                                                                                                                                                     aaTACGGGCAGACAGGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAg                                                                                                                                                                                                    <  2:157177/111‑1 (MQ=255)                                                                                                                                                                                         cGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGCTGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGGCCTGGAAGCCGCAGCGcccc                                                    >  2:242995/1‑249 (MQ=255)                                                                                                                                                                                           ggcggACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGCCCAACTGGAAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAAGCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTg                                                  <  2:418289/247‑1 (MQ=255)                                                                                                                                                                                           ggCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGCATTCCGCCGATACTGACGGGCTCCAGGAgtcgtc                                                                                                                                                                                            >  2:55153/1‑113 (MQ=255)                                                                                                                                                                                           ggCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAgtcgtc                                                                                                                                                                                            <  1:55153/113‑1 (MQ=255)                                                                                                                                                                                             cAGACATGGCCTACCCGGTTATTATTATTTTTGACACCTGACCAACTGGTAATGTTCGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCCGCAGCTGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGGTGTTGCACTGGGAGTCGCCGCGCCAATGgt                                                >  2:345038/1‑251 (MQ=255)                                                                                                                                                                                                 cATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTgg                                            >  1:344850/1‑251 (MQ=255)                                                                                                                                                                                                  atgtccTGCCCGGTTATCATTATTTTTGACACCAGAACAACTGGACATGTTAGCGACCGGCGCTCAGTTGGGATTCCGCCGATACTGGCGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCCGCCATGTGCCTTCTTCAGCGCGCAGCAGGTGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGAAGCGGCTAATGTTGCACTGGAAATCGCCGCGCCACTGGTGTggg                                           >  2:458573/5‑251 (MQ=255)                                                                                                                                                                                                  aTGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTggg                                           >  1:340701/1‑251 (MQ=255)                                                                                                                                                                                                      ccTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCAt                                       <  1:86646/251‑1 (MQ=255)                                                                                                                                                                                                       cTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGCAGTCGCCGCGCCACTGGTGTGGGCCAt                                       >  1:352539/1‑250 (MQ=255)                                                                                                                                                                                                          cccGGTTATTATTATTTTTGCCACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCCGTTGCTGTTGACTGTAGCGGCTGATGTTGATCTGGAAGTCGCCGCGCCAATGGGGTGGGCCATaatt                                   >  2:50775/1‑251 (MQ=255)                                                                                                                                                                                                                tattattatTTTTGACCCCAGACCAACTGGTAATGTTATCTACCGGCGCTCAGTGGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAATCCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCTGCAGATGGCGATGGTTGGTTTCCATCAGTTGCTGGTGACGGGAGCGGCTGATGTTTAACTGGAAGTCGCCGCGCCACTGGTGGTGGCCATaattcaatt                              <  2:452355/250‑1 (MQ=255)                                                                                                                                                                                                                        tttttGAAACCAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGACGTCGCCGCGCCACTGGTGTGTGCCATAATTCAATTCGCGCGTcc                     >  1:43029/1‑251 (MQ=255)                                                                                                                                                                                                                             gaccccAGACCAACGGGAAATGTTAGCGACCGCCGCCCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGACTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcgca                 <  2:398070/246‑1 (MQ=255)                                                                                                                                                                                                                                 ccAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcgcagcgc             >  1:348765/1‑250 (MQ=255)                                                                                                                                                                                                                                  cAGACCAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcgcagcgc             >  1:292822/1‑249 (MQ=255)                                                                                                                                                                                                                                  cAGACCAACTGGTAAGGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCcgcagcgcag           <  1:456722/251‑1 (MQ=255)                                                                                                                                                                                                                                   agcccAACTGTAAATGTTAGCGACCGGCGCCCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGa          <  2:43029/248‑1 (MQ=255)                                                                                                                                                                                                                                       cAACTGGTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGtgtg                                             >  1:280238/1‑212 (MQ=255)                                                                                                                                                                                                                                         acgggTAATGTTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGGTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGttt    <  1:21656/248‑1 (MQ=255)                                                                                                                                                                                                                                           tGGTAATGTTAGCGACCGGCGCCCAGCTGGAATTCCGCCGAAACTGACGGGCTACAGGAGTCGTCGCCACCAATACCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGCGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGCTGTTGATCTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGAGTCCCGCCGCGCAGCCCGTTTTc  >  2:427263/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   AGAGAAATAGCGGCAAAAATAATACCCGTATCACTTTTGCTGATATGGTTGATGTCATGTAGCCAAATCGGGAAAAACGGGAAGTAGGCTCCCATGATAAAAAAGTAAAAGAAAAAGAATAAACCGAACATCCAAAAGTTTGTGTTTTTTAAATAGTACATAATGGATTTCCTTACGCGAAATACGGGCAGACATGGCCTGCCCGGTTATTATTATTTTTGACACCAGACCAACTGGTAATGGTAGCGACCGGCGCTCAGCTGGAATTCCGCCGATACTGACGGGCTCCAGGAGTCGTCGCCACCAATCCCCATATGGAAACCGTCGATATTCAGCCATGTGCCTTCTTCCGCGTGCAGCAGATGGCGATGGCTGGTTTCCATCAGTTGCTGTTGACTGTAGCGGCTGATGTTGAACTGGAAGTCGCCGCGCCACTGGTGTGGGCCATAATTCAATTCGCGCGTCCCGCAGCGCAGACCGTTTTC  >  NC_000913/363019‑363503

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑