Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,510,2380TG94.9% 123.5 / ‑4.0 39intergenic (+445/+452)insO/fecEIS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base G (18/19);  total (18/21)
Fisher's exact test for biased strand distribution p-value = 4.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01

TGCTTCGCGCTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTG  >  NC_000913/4509991‑4510484
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
tGCTTCGCGCTTTCCCTCTGGCGAAAAAGGGCGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGt                                                                                                                                                                                                                                                     >  2:231395/1‑251 (MQ=255)
 gCTTCGCGCTTTACCTCTGGCGACAAAGGGAGCAGGACGGGCCCGTCTGCCTTCAATTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAACGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCGGAAACTGGATTAGCACTTCCGTTCCAGGGtt                                                                                                                                                                                                                                                    >  2:251384/1‑251 (MQ=255)
    tctcgcTTTCCGTCTGGCGAAAAATGTAGAAGTACGGTCCCCGCTGTCTTCTCTTTATGCCCGCTTCGGTAAGCTGTGTGAGACACCGACTCATTAAGATATCATTGATAACAATCCATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAATCTGCTCCTGCCTAAGTAGGAGCAAACGCACACTTTTTAGATAAATCGATGGGCAGAACCTGGATTAGCAATCCCGTTCTATGTTTg                                                                                                                                                                                                                                                   <  2:423550/246‑1 (MQ=255)
     cgcgCTTTCCCTCTGGCGAAAAAGGGAGAAGGAAGGGCCAGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACCATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCtt                                                                                                                                                                                                                                                >  1:34609/1‑251 (MQ=255)
                  tGGCGAAAAAGGGAGAAGGACGGGCACGTCTGCCTTCACTTTATGCCAGCTTCGGTACGCTGGGTGAGACACCGACTCCTGAAGATATCATTGATAACAATCGATACATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCCCACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTg                                                                                                                                                                                                                                   >  2:414289/1‑251 (MQ=255)
                   ggCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTGAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTgg                                                                                                                                                                                                                                  <  2:72910/251‑1 (MQ=255)
                    gCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGc                                                                                                                                                                                                                                 <  1:183680/251‑1 (MQ=255)
                      gAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGt                                                                                                                                                                                                                               <  1:231395/251‑1 (MQ=255)
                        aaaaGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCt                                                                                                                                                                                                                             <  2:257079/251‑1 (MQ=255)
                          aaGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAAAGTGGCTGCCGGTGATTAAGAAAGCTGCACCAACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGACACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTg                                                                                                                                                                                                                            >  2:306153/1‑250 (MQ=255)
                            gggAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCTGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATc                                                                                                                                                                                                                         <  1:242678/251‑1 (MQ=255)
                                                                    ttCGGTAAGCTGGGTTAGACACCGACTCATGACGATTTCATTGATATAAATCGATCCATCAACTGGCCTGTATAACATCGCTGCCGGTGATTCAGACAGCTGCACCTACCTAAGTCGTAGCAAACGCACACTTTTTAGAACAATCGTTGGTCAGAAACTGGATTAGCTGTTACGTTCCCGGGTTGCTTTTGATTTACGTTGGTGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGa                                                                                                                                                                                  >  2:165083/1‑250 (MQ=255)
                                                                            gCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTcc                                                                                                                                                                         <  1:222815/251‑1 (MQ=255)
                                                                               gggTGATACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAATCGGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATGCCGTTCCAGGGTTGTTTTTGATTTACGTTGGCGTCTGATCATGGAGTTATCCTCAAAAGCCCAACCTCATTGGTAATGATCCAGCTCCGTg                                                                                                                                                                      <  2:266352/251‑1 (MQ=255)
                                                                                          cGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGctc                                                                                                                                                           >  1:2726/1‑251 (MQ=255)
                                                                                                              gATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAAttttt                                                                                                                                       >  2:75193/1‑251 (MQ=255)
                                                                                                                   cAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGAtc                                                                                                                                  <  2:90109/251‑1 (MQ=255)
                                                                                                                          tCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTcccccc                                                                                                                           >  1:281304/1‑251 (MQ=255)
                                                                                                                           ccATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCt                                                                                                                          <  2:415082/251‑1 (MQ=255)
                                                                                                                               cAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTACGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGaa                                                                                                                       >  1:399591/1‑250 (MQ=255)
                                                                                                                                           ataACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTATTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGTGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTatt                                                                                                          <  2:147101/251‑3 (MQ=255)
                                                                                                                                                gTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGt                                                                                                     <  1:75792/251‑1 (MQ=255)
                                                                                                                                                       ccGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGttt                                                                                              >  1:80356/1‑251 (MQ=255)
                                                                                                                                                                  gAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCt                                                                                    >  2:372679/1‑250 (MQ=255)
                                                                                                                                                                            cctacctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAAc                                                                         <  1:137649/251‑1 (MQ=255)
                                                                                                                                                                              tacctaTGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACtt                                                                       >  1:117872/1‑251 (MQ=255)
                                                                                                                                                                                   aaGTAGGAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTcgc                                                                    <  2:246373/249‑1 (MQ=255)
                                                                                                                                                                                   aaGTAGGAGCAAACGCACACTTTTTAGAAAAAGCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATGCTCAAAAGCCCAACCGCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGAGCTCCCCCCTGAAAGCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGt                                                                  <  2:217027/251‑1 (MQ=255)
                                                                                                                                                                                        gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATACTTGATTTATCCTCAAAAGACCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCAACCTGCAATCTAAACTTCGTCATGTCAAGTTTTTGGGTTTCTAAAATTTTACCTTCGCGTTTTTc                                                             >  2:193292/1‑251 (MQ=255)
                                                                                                                                                                                        gtagCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACGGGATTAGCAATTCCGTTCCAGGGTTGCTTGTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAAACTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTc                                                             <  2:80212/251‑1 (MQ=255)
                                                                                                                                                                                             aaaCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGc                                                        >  1:297467/1‑251 (MQ=255)
                                                                                                                                                                                              aaCGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGcc                                                       <  1:75193/251‑1 (MQ=255)
                                                                                                                                                                                                              aaaaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATAc                                          >  1:149610/1‑248 (MQ=255)
                                                                                                                                                                                                              aaaaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGa                                       <  2:353964/251‑1 (MQ=255)
                                                                                                                                                                                                                aaaTCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCGAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGaaa                                     <  1:362162/251‑1 (MQ=255)
                                                                                                                                                                                                                                   ggATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAATGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTGTGATCTCCCCCCTGAAATCTAAACTTAGTCATGGCACGTTTTTGGGTTTCTAAAATTTTAACGTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATTTCCGTTAAGCAGAGt                    <  1:151612/249‑1 (MQ=255)
                                                                                                                                                                                                                                   ggATTAGCAATTCCGTTCCAGGGTTGCTTTTGAGTTACGTTGGCGTCTGATCATTGATTTTTCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGGGAATGTCCGCTCTGGCACAGGGCGAAATTTTTTGATCTCCCCCCGGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCTTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGtt                   <  1:251384/250‑1 (MQ=255)
                                                                                                                                                                                                                                           aaTTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAattg           >  2:394799/1‑250 (MQ=255)
                                                                                                                                                                                                                                                   tCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTg  >  1:422307/1‑251 (MQ=255)
                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      
TGCTTCGCGCTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTGCCGTAAGGGTTATACAGAAATGTCCGTTAAGCAGAGTTCAAAATTGATTGCCGTG  >  NC_000913/4509991‑4510484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑