Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,883	G→A	intergenic (‑178/+733)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,883	0	G	A	100.0%	46.8 / NA	16	intergenic (‑178/+733)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/10);  total (6/10)

GAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAA  >  NC_000913/3707661‑3708125                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   gagaCCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACa                                                                                                                                                                                                                         <  1:423834/250‑1 (MQ=255)                          tttttCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGATTGTCGCTGACTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGAGCGGATCCTCCCTAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAACACGTCAATACAGCCAACCGGGATTTACACCAACgggg                                                                                                                                                                                               >  2:351797/1‑249 (MQ=255)                           ttttCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGt                                                                                                                                                                                                <  1:34508/249‑1 (MQ=255)                              tCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTgaga                                                                                                                                                                                            >  2:370981/1‑250 (MQ=255)                                               attCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTGTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCGACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGAtt                                                                                                                                                                           <  2:16432/250‑1 (MQ=255)                                                                      gttgttTTAGCCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTg                                                                                                                                                   <  1:142143/251‑1 (MQ=255)                                                                                cccTTTGCAGGGGGTTGTCTCTGTCTGACTTTTTTGGTTTATTGCACGGTCGGGTCAACGTTATGAGGTGGGGATTCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTAGCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTTAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGaa                                                                                                                                         <  1:187378/251‑1 (MQ=255)                                                                                                               tttttgtttattgCCCGGTCGCGTCAACGTTATGAGGTGGAGATGCCGTACTACTTAACATCAGTGTAACAGTGCGGCTCCTCCATAAAATGCCCCTCGTGACCTAAAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACAGGGATTTACACCCACGGTGAGAATCCACAAACAAAATTTAAAAAAACTTCCAACAGCTATTTGAAGCAGAGAAATTTGTGCTAATCCAACATGACCAGacaaat                                                                                                          >  2:297790/1‑246 (MQ=255)                                                                                                                            cctggTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCATCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATAttt                                                                                             <  2:355536/248‑1 (MQ=255)                                                                                                                                           gTTATGAGGTGGGGATGACGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCGCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGGGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTAGTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGa                                                                              <  2:277849/251‑1 (MQ=255)                                                                                                                                               tGAGGTGGGGATCCCGTCCTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTCCAATCTATCAACAGAATGTGACAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTa                                                                           >  2:451843/1‑250 (MQ=255)                                                                                                                                                                aCTAATTAACATCAGTGTAACAGTGCGGATCCGCCATAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAAGCCACAAACAAAGATTAAAAAAACGTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATAGTTAGCAACATTTGGTGATTAAGGTTTATGCATTTAAt                                                          <  2:429566/250‑1 (MQ=255)                                                                                                                                                                                                    tAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACa                                                                                                                                                           >  1:246539/1‑117 (MQ=255)                                                                                                                                                                                                    tAAAATGCCCCTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACa                                                                                                                                                           <  2:246539/117‑1 (MQ=255)                                                                                                                                                                                                             ccTCGTGACCTACAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCAt            <  2:5179/251‑1 (MQ=255)                                                                                                                                                                                                                        aCAATCTATCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTaaa  >  2:12078/1‑250 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   GAGACCAAGCTTCAGCATCCCTGACTTTTTCAAGGAAATACGCATTATTCTGCTCCAATTGTGATGTTTGTTGTTTTAACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAA  >  NC_000913/3707661‑3708125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑