Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,388,041	T→G	T50P (ACG→CCG)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,388,041	0	T	G	100.0%	101.6 / NA	33	T50P (ACG→CCG)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (14/19);  total (14/19)

AATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCG‑ATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACC  >  NC_000913/3387796‑3388278                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                aaTGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcgg                                                                                                                                                                                                                                            >  1:337986/1‑251 (MQ=255)    gATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACcggcgg                                                                                                                                                                                                                                         >  2:235887/1‑251 (MQ=255)        aGCGCGATATAACCGGCCAGCCCCCACGCATACGTGTCTTCTATTCGTACCGGCGAGGCTACCCAGCTACAAACACCGGCCCCGATACAGCACACCAGCATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGCCCGGCACTACCGCCCATACATGTGCCGATGATGCGCAACAAGCCACGATAGCGACTAGCGCCAGAATACGGTTCACCTCCCGCAGCACAGGCCCGACCGGCGGcaac                                                                                                                                                                                                                                     >  2:179657/1‑251 (MQ=255)                                cACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACCCCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCa                                                                                                                                                                                                             <  1:62601/251‑1 (MQ=255)                                        cGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCg                                                                                                                                                                                                     <  1:108099/251‑1 (MQ=255)                                                     cGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGa                                                                                                                                                                                         <  1:20200/250‑1 (MQ=255)                                                         caaGCGCGGATATCCAGGTACAAAAACAGGCCCAGATACAGCACACCAGAATCATCCATACTGGAGCGCGGATCGTCGCAATAATGATCACCAGTCCGGCAATACAGCCAATAACTGGGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATCCGGTTAACCTCCCGCAGCAAAGGCCGGACCGGGGGCAACGATAGCCGCTGTCCGACCCGCCCAGCGGGGCGTTTcccgatggcaggg                                                                                                                                                                                     >  2:132847/3‑239 (MQ=255)                                                                      ccAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCaacaaaca                                                                                                                                                                       <  1:252294/251‑1 (MQ=255)                                                                        aGGTACAAAAAACGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGCCTCATCGCCATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGCTAGCGCATAGCGCCAGAATACGGTTCACCTCCCGCAGCAACGGCCGGACCGGCGGCAACAATCGCCGCTCTCAGTACCGCCCAGCGTTGCGTTTCCAGCTGGAAGTGAACGCCcacaaaccac                                                                                                                                                                     >  2:259085/1‑248 (MQ=255)                                                                            acaacacccGGCCCAGATACAGCATACCAGAAACATCAATAATGGTGCGCGGATCATCGCAATGATGAGCCCAATTCCGGCAAAACAGCCAAGAAAGTTGCCGCGGATGCGCAAAAAGCCACGATAGCGAATAGCGTCAGAACACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAGCAATTGCCGCTGTCACTACCGCCCAGCGTGTCTTGTCCAGCTGGAAGTGAAATCTAACAAACATCGccc                                                                                                                                                                 <  2:203012/244‑2 (MQ=255)                                                                                  aCCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAAGCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACaa                                                                                                                                                           <  1:172689/251‑1 (MQ=255)                                                                                                         gAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGATTTTCCGcc                                                                                                                                    >  2:398379/1‑250 (MQ=255)                                                                                                                caATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGaa                                                                                                                             <  1:70176/251‑1 (MQ=255)                                                                                                                      tGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCCCGATAGCGAATAGCGCCCGAATACGGTTCACCTCCCGCAGCACAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCCGCTGGAAGTGAAAGCCAACAACCAGCGCCAGTCCAATGGCTGTCTCCACtttttcccccaacccgctatttt                                                                                                                       >  2:213800/1‑232 (MQ=255)                                                                                                                       ggTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGtt                                                                                                                       <  1:105025/250‑1 (MQ=255)                                                                                                                        gTGCGCGGATCATCGCAATGATGATCACCAGTACGGTAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCAAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATGTGTTgg                                                                                                                     <  1:131167/251‑1 (MQ=255)                                                                                                                              ggATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGGATATGGTGGTTAGc                                                                                                                >  1:74372/1‑250 (MQ=255)                                                                                                                                         atgatgATCACCAGTCCGGCAATACAGCCAATAAATGGGCCGATGATGCGCAAAAAGCCACGATTGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGGGTGGCGGGTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAAGATGTTGGTTAGCAATTGAGAAAAt                                                                                                    <  2:171504/251‑1 (MQ=255)                                                                                                                                            atgatCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATAcc                                                                                                 <  1:398379/251‑1 (MQ=255)                                                                                                                                                 cACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTACCGCAAAGAGAATATGTTGGTTAGCAATGGAGAAAATACCCAt                                                                                              >  1:443148/1‑249 (MQ=255)                                                                                                                                                          ggCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATAGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTACCGCAAAGAGAATATGTTGGTTAGCAATGGGGAAAATAACCATCGGG‑ATTAAcc                                                                                  >  1:336035/1‑251 (MQ=255)                                                                                                                                                              aTACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTTCCGCAAAGCGAATATGTTGGTTAGCAATGGGGAAAATACCCCTCGTG‑ATTAACCAAAc                                                                              >  1:430777/1‑251 (MQ=255)                                                                                                                                                                         aaaTGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTATCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCg                                                                   <  1:63762/251‑1 (MQ=255)                                                                                                                                                                                               aaGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGGGAAAGCAAAAAAACACCGCCAGGACAATGGCGGTCGCCAGTTTTACCCCAAAGGGAATATGTTGGTTAGCCATGGAGAAAAAACCCATC‑TGTATTAACCAAAC‑CCCCCAAGCGGATGGGCCCTTTTACGgaaaaa                                             >  1:104027/1‑247 (MQ=255)                                                                                                                                                                                                       aTAGCGAATAGCGCCAGAATACGTTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATTGCGGTCGCCAGTTTGACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCAGCGTG‑ATTAACCAAACTCACGCAGGCG‑ATGGGCCATTTTACGGAAGAACGAATCCTg                                    <  1:229502/251‑1 (MQ=255)                                                                                                                                                                                                       aTAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGGG‑ATGGGCCATTTTACGGAAGAACGAATCCTg                                    <  1:420565/251‑1 (MQ=255)                                                                                                                                                                                                         aGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGACGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCCGTTTTACCGCAAAGCGAATATGTTGGTTAGCACTGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCG‑GTGGGCCATGTTACGGGAGAACGAATCCTGGc                                  >  1:83488/1‑251 (MQ=255)                                                                                                                                                                                                                aGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCG‑ATGGGCCATTTTACGGAAGAACGAATCCTGGCTTtcgtc                           <  2:322518/251‑1 (MQ=255)                                                                                                                                                                                                                       gAATACGGTGCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑AGTAACCAAACTCACGCAGGCG‑ATGGGCCAGTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCt                    <  1:288574/251‑1 (MQ=255)                                                                                                                                                                                                                        aaTACGGTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTGTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATCCCCATCGTG‑ATTAACCAAACTCACGCAGGCG‑ATGGGCCATTTTACGGAAGAGCGAATCCTGGCTTTCGTCGCGATCtt                   <  1:179657/251‑1 (MQ=255)                                                                                                                                                                                                                             ggTTCACCTCCCGCAGCAAAGGCCGGACCGGCGGTAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCG‑ATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTg              <  1:426882/251‑1 (MQ=255)                                                                                                                                                                                                                              gTTCCCCTCCCGCAGCAAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCG‑ATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGc             <  2:280636/251‑1 (MQ=255)                                                                                                                                                                                                                                         gcagcaAAGGCCGGACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGGTTTTCCGCAAAACGAATATGGTGGGTAGCAATGGAGAAAATACCCATCGTG‑ATTTACCAAACTCACGCAGGCG‑GTGGGCCCTTTTACGGGAAAAAGGATCCCTGCCTTCCTCCCGATCTTGTTTggcacttaacacc  >  2:328551/1‑239 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                AATGATCAGCGCGGTATAACCGGCCAGCCCCCACGCATACGAGTTTTCTATTCGTACCAGCGAGGATATCCAGGTACAAAAACCGGCCCAGATACAGCACACCAGAATCATCAATAATGGTGCGCGGATCATCGCAATGATGATCACCAGTCCGGCAATACAGCCAATAAATGTGCCGATGATGCGCAAAAAGCCACGATAGCGAATAGCGCCAGAATACGGTTCACCTCCCGCAGCAAAGGCCGTACCGGCGGCAACAATCGCCGCTGTCAGTACCGCCCAGCGTGGCGTTTCCAGCTGGAAGTGAAAGCCAACAAACAGCGCCAGTACAATGGCGGTCGCCAGTTTTACCGCAAAGCGAATATGTTGGTTAGCAATGGAGAAAATACCCATCGTG‑ATTAACCAAACTCACGCAGGCG‑ATGGGCCATTTTACGGAAGAACGAATCCTGGCTTTCGTCGCGATCTTGTTTGCCAGTGACCACC  >  NC_000913/3387796‑3388278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑